TY  - JOUR
AU  - Janković, Vukašin
AU  - Marković, Darka
AU  - Nikodinović-Runić, Jasmina
AU  - Radetić, Maja
AU  - Ilić-Tomić, Tatjana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5276
AB  - Given the environmental burden of textile industry, especially of dyeing processes and the volume of synthetic dyes and surfactants, the intensive development of the greener approaches is under way. Herein, an environmentaly-friendly dyeing of polyamide (PA) and PA/Elastane (PA/EA) knits using live bacterial approach in water environment, completely eliminating usage of textile auxiliaries is described. A total of 12 pigment-producing Streptomyces strains were isolated and purified from soil and rizoshere or bark of smoke tree Cotinus coggygria samples. The antibacterial, antifungal and cytotoxic effects of crude bacterial extracts were tested. Antimicrobial effect was obtained by the majority of extracts but only two streptomycetes extracts, 11–5 and BPS51, showed moderate cytotoxicity against HaCaT human cell line. This was the reason to select 11–5 and BPS51 strains for the dyeing of the textile materials. Excellent properties of dyeing wool, silk and PA are achieved initially using live cultures, and the bioprocess is optimized on commercial PA and PA/EA knits used for stockings production. Satisfactory coloration of both knits is achieved with dynamic conditions (culture shaking at 180 rpm over 5–14 days at 30 ºC) giving the best coloration results, except in the case of the PA sample dyed with a bacterial strain 11–5. The prolongation of dyeing time leads to higher color yields independently of fabric and bacteria strain. Although the color differences between the samples before and after washing are observed, washing fastness after three washing cycles can be considered as satisfactory.
PB  - Springer Science and Business Media
T2  - World Journal of Microbiology and Biotechnology
T1  - Eco‑friendly dyeing of polyamide and polyamide‑elastane knits with living bacterial cultures of two Streptomyces sp. strains
IS  - 1
SP  - 32
VL  - 39
DO  - 10.1007/s11274-022-03473-4
ER  - 

@article{
author = "Janković, Vukašin and Marković, Darka and Nikodinović-Runić, Jasmina and Radetić, Maja and Ilić-Tomić, Tatjana",
year = "2023",
abstract = "Given the environmental burden of textile industry, especially of dyeing processes and the volume of synthetic dyes and surfactants, the intensive development of the greener approaches is under way. Herein, an environmentaly-friendly dyeing of polyamide (PA) and PA/Elastane (PA/EA) knits using live bacterial approach in water environment, completely eliminating usage of textile auxiliaries is described. A total of 12 pigment-producing Streptomyces strains were isolated and purified from soil and rizoshere or bark of smoke tree Cotinus coggygria samples. The antibacterial, antifungal and cytotoxic effects of crude bacterial extracts were tested. Antimicrobial effect was obtained by the majority of extracts but only two streptomycetes extracts, 11–5 and BPS51, showed moderate cytotoxicity against HaCaT human cell line. This was the reason to select 11–5 and BPS51 strains for the dyeing of the textile materials. Excellent properties of dyeing wool, silk and PA are achieved initially using live cultures, and the bioprocess is optimized on commercial PA and PA/EA knits used for stockings production. Satisfactory coloration of both knits is achieved with dynamic conditions (culture shaking at 180 rpm over 5–14 days at 30 ºC) giving the best coloration results, except in the case of the PA sample dyed with a bacterial strain 11–5. The prolongation of dyeing time leads to higher color yields independently of fabric and bacteria strain. Although the color differences between the samples before and after washing are observed, washing fastness after three washing cycles can be considered as satisfactory.",
publisher = "Springer Science and Business Media",
journal = "World Journal of Microbiology and Biotechnology",
title = "Eco‑friendly dyeing of polyamide and polyamide‑elastane knits with living bacterial cultures of two Streptomyces sp. strains",
number = "1",
pages = "32",
volume = "39",
doi = "10.1007/s11274-022-03473-4"
}

Janković, V., Marković, D., Nikodinović-Runić, J., Radetić, M.,& Ilić-Tomić, T.. (2023). Eco‑friendly dyeing of polyamide and polyamide‑elastane knits with living bacterial cultures of two Streptomyces sp. strains. in World Journal of Microbiology and Biotechnology
Springer Science and Business Media., 39(1), 32.
https://doi.org/10.1007/s11274-022-03473-4

Janković V, Marković D, Nikodinović-Runić J, Radetić M, Ilić-Tomić T. Eco‑friendly dyeing of polyamide and polyamide‑elastane knits with living bacterial cultures of two Streptomyces sp. strains. in World Journal of Microbiology and Biotechnology. 2023;39(1):32.
doi:10.1007/s11274-022-03473-4 .

Janković, Vukašin, Marković, Darka, Nikodinović-Runić, Jasmina, Radetić, Maja, Ilić-Tomić, Tatjana, "Eco‑friendly dyeing of polyamide and polyamide‑elastane knits with living bacterial cultures of two Streptomyces sp. strains" in World Journal of Microbiology and Biotechnology, 39, no. 1 (2023):32,
https://doi.org/10.1007/s11274-022-03473-4 . .

TY  - JOUR
AU  - Krkobabić, Ana
AU  - Radetić, Maja
AU  - Tseng, Hsiang-Han
AU  - Nunney, Tim S.
AU  - Tadić, Vanja
AU  - Ilić-Tomić, Tatjana
AU  - Marković, Darka
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5286
AB  - The demand for medical textiles in various forms with strong antimicrobial activity drastically increased during the COVID19 pandemic. In an attempt to tackle this issue and to develop antimicrobial textiles in more environmentally benign manner, a viscose fabric after coating with biopolymer chitosan has been impregnated with Ag- and Cu-based nanoparticles. Chitosan was applied in the presence and absence of cross-linker 1,2,3,4-butanetetracarboxylic acid (BTCA). In situ green synthesis of nanoparticles was performed using a Punica granatum (pomegranate) peel extract as a reducing and stabilizing agent. Formation of nanoparticles on the fiber surface was confirmed by FESEM. Elemental analysis by XPS showed the synthesized nanoparticles exist as AgCl and a mixture of Cu/CuO/Cu2O in the modified samples. Moreover, these nanoparticles appeared to be present not only on the sample surface but also buried within the fibers, as indicated by XPS mapping and depth profiling measurements. All impregnated fabrics exhibited excellent antifungal activity providing the maximum reduction of yeast Candida albicans colonies. Antibacterial activity was stronger against Gram-negative bacteria Escherichia coli than Gram-positive bacteria Staphylococcus aureus, and it was highly influenced by metal content. The fabrics impregnated with AgCl nanoparticles showed lower cytotoxicity towards human keratinocyte cells.
PB  - Elsevier B.V.
T2  - Applied Surface Science
T1  - Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract
SP  - 155612
VL  - 611
DO  - 10.1016/j.apsusc.2022.155612
ER  - 

@article{
author = "Krkobabić, Ana and Radetić, Maja and Tseng, Hsiang-Han and Nunney, Tim S. and Tadić, Vanja and Ilić-Tomić, Tatjana and Marković, Darka",
year = "2023",
abstract = "The demand for medical textiles in various forms with strong antimicrobial activity drastically increased during the COVID19 pandemic. In an attempt to tackle this issue and to develop antimicrobial textiles in more environmentally benign manner, a viscose fabric after coating with biopolymer chitosan has been impregnated with Ag- and Cu-based nanoparticles. Chitosan was applied in the presence and absence of cross-linker 1,2,3,4-butanetetracarboxylic acid (BTCA). In situ green synthesis of nanoparticles was performed using a Punica granatum (pomegranate) peel extract as a reducing and stabilizing agent. Formation of nanoparticles on the fiber surface was confirmed by FESEM. Elemental analysis by XPS showed the synthesized nanoparticles exist as AgCl and a mixture of Cu/CuO/Cu2O in the modified samples. Moreover, these nanoparticles appeared to be present not only on the sample surface but also buried within the fibers, as indicated by XPS mapping and depth profiling measurements. All impregnated fabrics exhibited excellent antifungal activity providing the maximum reduction of yeast Candida albicans colonies. Antibacterial activity was stronger against Gram-negative bacteria Escherichia coli than Gram-positive bacteria Staphylococcus aureus, and it was highly influenced by metal content. The fabrics impregnated with AgCl nanoparticles showed lower cytotoxicity towards human keratinocyte cells.",
publisher = "Elsevier B.V.",
journal = "Applied Surface Science",
title = "Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract",
pages = "155612",
volume = "611",
doi = "10.1016/j.apsusc.2022.155612"
}

Krkobabić, A., Radetić, M., Tseng, H., Nunney, T. S., Tadić, V., Ilić-Tomić, T.,& Marković, D.. (2023). Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract. in Applied Surface Science
Elsevier B.V.., 611, 155612.
https://doi.org/10.1016/j.apsusc.2022.155612

Krkobabić A, Radetić M, Tseng H, Nunney TS, Tadić V, Ilić-Tomić T, Marković D. Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract. in Applied Surface Science. 2023;611:155612.
doi:10.1016/j.apsusc.2022.155612 .

Krkobabić, Ana, Radetić, Maja, Tseng, Hsiang-Han, Nunney, Tim S., Tadić, Vanja, Ilić-Tomić, Tatjana, Marković, Darka, "Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract" in Applied Surface Science, 611 (2023):155612,
https://doi.org/10.1016/j.apsusc.2022.155612 . .

TY  - JOUR
AU  - Tomić, Simonida Lj.
AU  - Vuković, Jovana S.
AU  - Babić Radić, Marija M.
AU  - Filipović, Vuk V.
AU  - Živanović, Dubravka P.
AU  - Nikolić, Miloš M.
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5869
AB  - Scaffolding biomaterials are gaining great importance due to their beneficial properties for medical purposes. Targeted biomaterial engineering strategies through the synergy of different material types can be applied to design hybrid scaffolding biomaterials with advantageous properties for biomedical applications. In our research, a novel combination of the bioactive agent Manuka honey (MHo) with 2-hydroxyethyl methacrylate/gelatin (HG) hydrogel scaffolds was created as an efficient bioactive platform for biomedical applications. The effects of Manuka honey content on structural characteristics, porosity, swelling performance, in vitro degradation, and in vitro biocompatibility (fibroblast and keratinocyte cell lines) of hybrid hydrogel scaffolds were studied using Fourier transform infrared spectroscopy, the gravimetric method, and in vitro MTT biocompatibility assays. The engineered hybrid hydrogel scaffolds show advantageous properties, including porosity in the range of 71.25% to 90.09%, specific pH- and temperature-dependent swelling performance, and convenient absorption capacity. In vitro degradation studies showed scaffold degradability ranging from 6.27% to 27.18% for four weeks. In vitro biocompatibility assays on healthy human fibroblast (MRC5 cells) and keratinocyte (HaCaT cells) cell lines by MTT tests showed that cell viability depends on the Manuka honey content loaded in the HG hydrogel scaffolds. A sample containing the highest Manuka honey content (30%) exhibited the best biocompatible properties. The obtained results reveal that the synergy of the bioactive agent, Manuka honey, with 2-hydroxyethyl methacrylate/gelatin as hybrid hydrogel scaffolds has potential for biomedical purposes. By tuning the Manuka honey content in HG hydrogel scaffolds advantageous properties of hybrid scaffolds can be achieved for biomedical applications.
PB  - MDPI
T2  - Polymers
T1  - Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration
IS  - 3
SP  - 589
VL  - 15
DO  - 10.3390/polym15030589
ER  - 

@article{
author = "Tomić, Simonida Lj. and Vuković, Jovana S. and Babić Radić, Marija M. and Filipović, Vuk V. and Živanović, Dubravka P. and Nikolić, Miloš M. and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Scaffolding biomaterials are gaining great importance due to their beneficial properties for medical purposes. Targeted biomaterial engineering strategies through the synergy of different material types can be applied to design hybrid scaffolding biomaterials with advantageous properties for biomedical applications. In our research, a novel combination of the bioactive agent Manuka honey (MHo) with 2-hydroxyethyl methacrylate/gelatin (HG) hydrogel scaffolds was created as an efficient bioactive platform for biomedical applications. The effects of Manuka honey content on structural characteristics, porosity, swelling performance, in vitro degradation, and in vitro biocompatibility (fibroblast and keratinocyte cell lines) of hybrid hydrogel scaffolds were studied using Fourier transform infrared spectroscopy, the gravimetric method, and in vitro MTT biocompatibility assays. The engineered hybrid hydrogel scaffolds show advantageous properties, including porosity in the range of 71.25% to 90.09%, specific pH- and temperature-dependent swelling performance, and convenient absorption capacity. In vitro degradation studies showed scaffold degradability ranging from 6.27% to 27.18% for four weeks. In vitro biocompatibility assays on healthy human fibroblast (MRC5 cells) and keratinocyte (HaCaT cells) cell lines by MTT tests showed that cell viability depends on the Manuka honey content loaded in the HG hydrogel scaffolds. A sample containing the highest Manuka honey content (30%) exhibited the best biocompatible properties. The obtained results reveal that the synergy of the bioactive agent, Manuka honey, with 2-hydroxyethyl methacrylate/gelatin as hybrid hydrogel scaffolds has potential for biomedical purposes. By tuning the Manuka honey content in HG hydrogel scaffolds advantageous properties of hybrid scaffolds can be achieved for biomedical applications.",
publisher = "MDPI",
journal = "Polymers",
title = "Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration",
number = "3",
pages = "589",
volume = "15",
doi = "10.3390/polym15030589"
}

Tomić, S. Lj., Vuković, J. S., Babić Radić, M. M., Filipović, V. V., Živanović, D. P., Nikolić, M. M.,& Nikodinović-Runić, J.. (2023). Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration. in Polymers
MDPI., 15(3), 589.
https://doi.org/10.3390/polym15030589

Tomić SL, Vuković JS, Babić Radić MM, Filipović VV, Živanović DP, Nikolić MM, Nikodinović-Runić J. Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration. in Polymers. 2023;15(3):589.
doi:10.3390/polym15030589 .

Tomić, Simonida Lj., Vuković, Jovana S., Babić Radić, Marija M., Filipović, Vuk V., Živanović, Dubravka P., Nikolić, Miloš M., Nikodinović-Runić, Jasmina, "Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration" in Polymers, 15, no. 3 (2023):589,
https://doi.org/10.3390/polym15030589 . .

TY  - JOUR
AU  - Ponjavić, Marijana
AU  - Malagurski, Ivana
AU  - Lazić, Jelena
AU  - Jeremić, Sanja
AU  - Pavlović, Vladimir
AU  - Prlainović, Nevena
AU  - Maksimović, Vesna
AU  - Ćosović, Vladan
AU  - Atanase, Leonard Ionut
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5866
AB  - The quest for sustainable biomaterials with excellent biocompatibility and tailorableproperties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high productioncosts and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with stronganticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. Thesamples were produced in the form of films 115.6–118.8  m in thickness using the solvent castingmethod. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinityand thermal stability) and functionality of the obtained biomaterials were investigated. PGhas acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability andmorphology of the films. All samples with PG had a more organized internal structure and highermelting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymerwas 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively.Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (coloncancer) cells, thus advancing PHBV biomedical application potential.
PB  - Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - International Journal of Molecular Sciences
T1  - Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin
SP  - 1906
VL  - 24
DO  - 10.3390/ijms24031906
ER  - 

@article{
author = "Ponjavić, Marijana and Malagurski, Ivana and Lazić, Jelena and Jeremić, Sanja and Pavlović, Vladimir and Prlainović, Nevena and Maksimović, Vesna and Ćosović, Vladan and Atanase, Leonard Ionut",
year = "2023",
abstract = "The quest for sustainable biomaterials with excellent biocompatibility and tailorableproperties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high productioncosts and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with stronganticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. Thesamples were produced in the form of films 115.6–118.8  m in thickness using the solvent castingmethod. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinityand thermal stability) and functionality of the obtained biomaterials were investigated. PGhas acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability andmorphology of the films. All samples with PG had a more organized internal structure and highermelting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymerwas 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively.Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (coloncancer) cells, thus advancing PHBV biomedical application potential.",
publisher = "Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "International Journal of Molecular Sciences",
title = "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin",
pages = "1906",
volume = "24",
doi = "10.3390/ijms24031906"
}

Ponjavić, M., Malagurski, I., Lazić, J., Jeremić, S., Pavlović, V., Prlainović, N., Maksimović, V., Ćosović, V.,& Atanase, L. I.. (2023). Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences
Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)., 24, 1906.
https://doi.org/10.3390/ijms24031906

Ponjavić M, Malagurski I, Lazić J, Jeremić S, Pavlović V, Prlainović N, Maksimović V, Ćosović V, Atanase LI. Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences. 2023;24:1906.
doi:10.3390/ijms24031906 .

Ponjavić, Marijana, Malagurski, Ivana, Lazić, Jelena, Jeremić, Sanja, Pavlović, Vladimir, Prlainović, Nevena, Maksimović, Vesna, Ćosović, Vladan, Atanase, Leonard Ionut, "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin" in International Journal of Molecular Sciences, 24 (2023):1906,
https://doi.org/10.3390/ijms24031906 . .

TY  - JOUR
AU  - Radonjić, Mia
AU  - Petrović, Jelena
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5261
AB  - A multidisciplinary approach based on experiments and mathematical modeling was used in biomimetic system development for three-dimensional (3D) cultures of cancer cells. Specifically, two cancer cell lines, human embryonic teratocarcinoma NT2/D1 and rat glioma C6, were immobilized in alginate microbeads and microfibers, respectively, and cultured under static and flow conditions in perfusion bioreactors. At the same time, chemical engineering methods were applied to explain the obtained results. The superficial medium velocity of 80 μm s-1 induced lower viability of NT2/D1 cells in superficial microbead zones, implying adverse effects of fluid shear stresses estimated as ∼67 mPa. On the contrary, similar velocity (100 μm s-1) enhanced the proliferation of C6 glioma cells within microfibers compared to static controls. An additional study of silver release from nanocomposite Ag/honey/alginate microfibers under perfusion indicated that the medium partially flows through the hydrogel (interstitial velocity of ∼10 nm s-1). Thus, a diffusion-advection-reaction model described the mass transport to immobilized cells within microfibers. Substances with diffusion coefficients of ∼10-9-10-11 m2 s-1 are sufficiently supplied by diffusion only, while those with significantly lower diffusivities (∼10-19 m2 s-1) require additional convective transport. The present study demonstrates the selection and contribution of chemical engineering methods in tumor model system development.
PB  - National Library of Serbia
T2  - Chemical Industry and Chemical Engineering Quarterly
T2  - Chemical Industry and Chemical Engineering Quarterly
T1  - Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations
EP  - 223
IS  - 3
SP  - 211
VL  - 28
DO  - 10.2298/CICEQ210607033R
ER  - 

@article{
author = "Radonjić, Mia and Petrović, Jelena and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2022",
abstract = "A multidisciplinary approach based on experiments and mathematical modeling was used in biomimetic system development for three-dimensional (3D) cultures of cancer cells. Specifically, two cancer cell lines, human embryonic teratocarcinoma NT2/D1 and rat glioma C6, were immobilized in alginate microbeads and microfibers, respectively, and cultured under static and flow conditions in perfusion bioreactors. At the same time, chemical engineering methods were applied to explain the obtained results. The superficial medium velocity of 80 μm s-1 induced lower viability of NT2/D1 cells in superficial microbead zones, implying adverse effects of fluid shear stresses estimated as ∼67 mPa. On the contrary, similar velocity (100 μm s-1) enhanced the proliferation of C6 glioma cells within microfibers compared to static controls. An additional study of silver release from nanocomposite Ag/honey/alginate microfibers under perfusion indicated that the medium partially flows through the hydrogel (interstitial velocity of ∼10 nm s-1). Thus, a diffusion-advection-reaction model described the mass transport to immobilized cells within microfibers. Substances with diffusion coefficients of ∼10-9-10-11 m2 s-1 are sufficiently supplied by diffusion only, while those with significantly lower diffusivities (∼10-19 m2 s-1) require additional convective transport. The present study demonstrates the selection and contribution of chemical engineering methods in tumor model system development.",
publisher = "National Library of Serbia",
journal = "Chemical Industry and Chemical Engineering Quarterly, Chemical Industry and Chemical Engineering Quarterly",
title = "Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations",
pages = "223-211",
number = "3",
volume = "28",
doi = "10.2298/CICEQ210607033R"
}

Radonjić, M., Petrović, J., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2022). Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations. in Chemical Industry and Chemical Engineering Quarterly
National Library of Serbia., 28(3), 211-223.
https://doi.org/10.2298/CICEQ210607033R

Radonjić M, Petrović J, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations. in Chemical Industry and Chemical Engineering Quarterly. 2022;28(3):211-223.
doi:10.2298/CICEQ210607033R .

Radonjić, Mia, Petrović, Jelena, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations" in Chemical Industry and Chemical Engineering Quarterly, 28, no. 3 (2022):211-223,
https://doi.org/10.2298/CICEQ210607033R . .

TY  - JOUR
AU  - Pavić, Aleksandar
AU  - Stojanović, Zoran
AU  - Pekmezović, Marina
AU  - Veljović, Đorđe
AU  - O’connor, Kevin
AU  - Malagurski, Ivana
AU  - Nikodinović-Runić, Jasmina
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5104
AB  - Immobilizing antifungal polyenes such as nystatin (Nys) and amphotericin B (AmB) into biodegradable formulations is advantageous compared to free drug administration providing sustained release, reduced dosing due to localized targeting and overall reduced systemic drug toxicity. In this study, we encapsulated Nys and AmB in medium chain length polyhydroxyalkanoates (mcl-PHA) microspheres (7–8 µm in diameter). The obtained formulations have been validated for antifungal activity in vitro against a panel of pathogenic fungi including species of Candida, Aspergillus, Microsporum and Trichophyton genera and toxicity and efficacy in vivo using the zebrafish model of disseminated candidiasis. While free polyenes, especially AmB, were highly toxic to zebrafish embryos at the effective (MIC) doses, after their loading into mcl-PHA microspheres, inner organ toxicity and teratogenicity associated with both drugs were not observed, even at 100 × MIC doses. The obtained mcl-PHA/polyene formulations have successfully eradicated C. albicans infection and showed an improved therapeutic profile in zebrafish by enhancing infected embryos survival. This approach is contributing to the antifungal arsenal as polyenes, although the first broad-spectrum antifungals on the market are still the gold standard for treatment of fungal infections.
PB  - MDPI
T2  - Pharmaceutics
T1  - Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model
IS  - 4
SP  - 696
VL  - 14
DO  - 10.3390/pharmaceutics14040696
ER  - 

@article{
author = "Pavić, Aleksandar and Stojanović, Zoran and Pekmezović, Marina and Veljović, Đorđe and O’connor, Kevin and Malagurski, Ivana and Nikodinović-Runić, Jasmina",
year = "2022",
abstract = "Immobilizing antifungal polyenes such as nystatin (Nys) and amphotericin B (AmB) into biodegradable formulations is advantageous compared to free drug administration providing sustained release, reduced dosing due to localized targeting and overall reduced systemic drug toxicity. In this study, we encapsulated Nys and AmB in medium chain length polyhydroxyalkanoates (mcl-PHA) microspheres (7–8 µm in diameter). The obtained formulations have been validated for antifungal activity in vitro against a panel of pathogenic fungi including species of Candida, Aspergillus, Microsporum and Trichophyton genera and toxicity and efficacy in vivo using the zebrafish model of disseminated candidiasis. While free polyenes, especially AmB, were highly toxic to zebrafish embryos at the effective (MIC) doses, after their loading into mcl-PHA microspheres, inner organ toxicity and teratogenicity associated with both drugs were not observed, even at 100 × MIC doses. The obtained mcl-PHA/polyene formulations have successfully eradicated C. albicans infection and showed an improved therapeutic profile in zebrafish by enhancing infected embryos survival. This approach is contributing to the antifungal arsenal as polyenes, although the first broad-spectrum antifungals on the market are still the gold standard for treatment of fungal infections.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model",
number = "4",
pages = "696",
volume = "14",
doi = "10.3390/pharmaceutics14040696"
}

Pavić, A., Stojanović, Z., Pekmezović, M., Veljović, Đ., O’connor, K., Malagurski, I.,& Nikodinović-Runić, J.. (2022). Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model. in Pharmaceutics
MDPI., 14(4), 696.
https://doi.org/10.3390/pharmaceutics14040696

Pavić A, Stojanović Z, Pekmezović M, Veljović Đ, O’connor K, Malagurski I, Nikodinović-Runić J. Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model. in Pharmaceutics. 2022;14(4):696.
doi:10.3390/pharmaceutics14040696 .

Pavić, Aleksandar, Stojanović, Zoran, Pekmezović, Marina, Veljović, Đorđe, O’connor, Kevin, Malagurski, Ivana, Nikodinović-Runić, Jasmina, "Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model" in Pharmaceutics, 14, no. 4 (2022):696,
https://doi.org/10.3390/pharmaceutics14040696 . .

TY  - JOUR
AU  - Assaleh, Mohamed H.
AU  - Jeremić, Sanja
AU  - Cvijetić, Ilija
AU  - Marinković, Aleksandar
AU  - Prlainović, Nevena
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5111
AB  - Antimicrobial-resistance (AMR) has become the greatest concern and highly challenging issue when treating nosocomial infections. The exigency to develop new potent compounds continues to increase worldwide, whereby derivatives of natural products are becoming more attractive. In the present paper, the microbiological assessment of a series of 12 cinnamide hydrazides, four of them completely novel, against clinically relevant pathogens has discovered several derivatives with promising in vitro activities against Acinetobacter baumannii, one of the most dreaded opportunistic pathogens in hospitals. The compounds were synthesized by combining one of three different natural acids (cinnamic, 4-chloro or 4-methoxy) with four monothiocarbohydrazones (MTCHs) - an important class of synthetic organic molecules. Their structure was confirmed by elemental microanalysis, as well as ATR-FTIR, 1H and 13C NMR spectra, with the addition of 2D NMR spectra for novel compounds. The hybrids of cinnamic acids and pyridine derivatives are particularly active compounds with the lowest MIC50 value of 10.4 µM for p-chloro cinnamic acid and acetyl pyridine derivatives. An alignment-independent 3D QSAR model identified pharmacophoric hotspots and suggested several structural modifications that might improve the potency of this class of compounds against A. baumannii. The compounds are strong iron-chelating agents forming complexes with a stability constant between 107 and 109. The synthesized derivatives represent a promising class of antibacterial compounds with activities comparable to the commonly used antibiotics.
PB  - Elsevier B.V.
T2  - Journal of Molecular Structure
T1  - In vitro activity of novel cinnamic acids hydrazides against clinically important pathogens
SP  - 133016
VL  - 1262
DO  - 10.1016/j.molstruc.2022.133016
ER  - 

@article{
author = "Assaleh, Mohamed H. and Jeremić, Sanja and Cvijetić, Ilija and Marinković, Aleksandar and Prlainović, Nevena",
year = "2022",
abstract = "Antimicrobial-resistance (AMR) has become the greatest concern and highly challenging issue when treating nosocomial infections. The exigency to develop new potent compounds continues to increase worldwide, whereby derivatives of natural products are becoming more attractive. In the present paper, the microbiological assessment of a series of 12 cinnamide hydrazides, four of them completely novel, against clinically relevant pathogens has discovered several derivatives with promising in vitro activities against Acinetobacter baumannii, one of the most dreaded opportunistic pathogens in hospitals. The compounds were synthesized by combining one of three different natural acids (cinnamic, 4-chloro or 4-methoxy) with four monothiocarbohydrazones (MTCHs) - an important class of synthetic organic molecules. Their structure was confirmed by elemental microanalysis, as well as ATR-FTIR, 1H and 13C NMR spectra, with the addition of 2D NMR spectra for novel compounds. The hybrids of cinnamic acids and pyridine derivatives are particularly active compounds with the lowest MIC50 value of 10.4 µM for p-chloro cinnamic acid and acetyl pyridine derivatives. An alignment-independent 3D QSAR model identified pharmacophoric hotspots and suggested several structural modifications that might improve the potency of this class of compounds against A. baumannii. The compounds are strong iron-chelating agents forming complexes with a stability constant between 107 and 109. The synthesized derivatives represent a promising class of antibacterial compounds with activities comparable to the commonly used antibiotics.",
publisher = "Elsevier B.V.",
journal = "Journal of Molecular Structure",
title = "In vitro activity of novel cinnamic acids hydrazides against clinically important pathogens",
pages = "133016",
volume = "1262",
doi = "10.1016/j.molstruc.2022.133016"
}

Assaleh, M. H., Jeremić, S., Cvijetić, I., Marinković, A.,& Prlainović, N.. (2022). In vitro activity of novel cinnamic acids hydrazides against clinically important pathogens. in Journal of Molecular Structure
Elsevier B.V.., 1262, 133016.
https://doi.org/10.1016/j.molstruc.2022.133016

Assaleh MH, Jeremić S, Cvijetić I, Marinković A, Prlainović N. In vitro activity of novel cinnamic acids hydrazides against clinically important pathogens. in Journal of Molecular Structure. 2022;1262:133016.
doi:10.1016/j.molstruc.2022.133016 .

Assaleh, Mohamed H., Jeremić, Sanja, Cvijetić, Ilija, Marinković, Aleksandar, Prlainović, Nevena, "In vitro activity of novel cinnamic acids hydrazides against clinically important pathogens" in Journal of Molecular Structure, 1262 (2022):133016,
https://doi.org/10.1016/j.molstruc.2022.133016 . .

TY  - JOUR
AU  - Vuković, Jovana S.
AU  - Filipović, Vuk V.
AU  - Babić Radić, Marija M.
AU  - Vukomanović, Marija
AU  - Milivojević, Dušan
AU  - Ilić-Tomić, Tatjana
AU  - Nikodinović-Runić, Jasmina
AU  - Tomić, Simonida Lj.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5242
AB  - Scaffold hydrogel biomaterials designed to have advantageous biofunctional properties, which can be applied for controlled bioactive agent release, represent an important concept in biomedical tissue engineering. Our goal was to create scaffolding materials that mimic living tissue for biomedical utilization. In this study, two novel series of interpenetrating hydrogel networks (IPNs) based on 2-hydroxyethyl methacrylate/gelatin and 2-hydroxyethyl methacrylate/alginate were crosslinked using N-ethyl-N′-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). Characterization included examining the effects of crosslinker type and concentration on structure, morphological and mechanical properties, in vitro swelling, hydrophilicity as well as on the in vitro cell viability (fibroblast cells) and in vivo (Caenorhabditis elegans) interactions of novel biomaterials. The engineered IPN hydrogel scaffolds show an interconnected pore morphology and porosity range of 62.36 to 85.20%, favorable in vitro swelling capacity, full hydrophilicity, and Young’s modulus values in the range of 1.40 to 7.50 MPa. In vitro assay on healthy human fibroblast (MRC5 cells) by MTT test and in vivo (Caenorhabditis elegans) survival assays show the advantageous biocompatible properties of novel IPN hydrogel scaffolds. Furthermore, in vitro controlled release study of the therapeutic agent resveratrol showed that these novel scaffolding systems are suitable controlled release platforms. The results revealed that the use of EDC and the combination of EDC/NHS crosslinkers can be applied to prepare and tune the properties of the IPN 2-hydroxyethyl methacrylate/alginate and 2-hydroxyethyl methacrylate/gelatin hydrogel scaffolds series, which have shown great potential for biomedical engineering applications.
PB  - MDPI
T2  - Polymers
T1  - In Vitro and In Vivo Biocompatible and Controlled Resveratrol Release Performances of HEMA/Alginate and HEMA/Gelatin IPN Hydrogel Scaffolds
IS  - 20
SP  - 4459
VL  - 14
DO  - 10.3390/polym14204459
ER  - 

@article{
author = "Vuković, Jovana S. and Filipović, Vuk V. and Babić Radić, Marija M. and Vukomanović, Marija and Milivojević, Dušan and Ilić-Tomić, Tatjana and Nikodinović-Runić, Jasmina and Tomić, Simonida Lj.",
year = "2022",
abstract = "Scaffold hydrogel biomaterials designed to have advantageous biofunctional properties, which can be applied for controlled bioactive agent release, represent an important concept in biomedical tissue engineering. Our goal was to create scaffolding materials that mimic living tissue for biomedical utilization. In this study, two novel series of interpenetrating hydrogel networks (IPNs) based on 2-hydroxyethyl methacrylate/gelatin and 2-hydroxyethyl methacrylate/alginate were crosslinked using N-ethyl-N′-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). Characterization included examining the effects of crosslinker type and concentration on structure, morphological and mechanical properties, in vitro swelling, hydrophilicity as well as on the in vitro cell viability (fibroblast cells) and in vivo (Caenorhabditis elegans) interactions of novel biomaterials. The engineered IPN hydrogel scaffolds show an interconnected pore morphology and porosity range of 62.36 to 85.20%, favorable in vitro swelling capacity, full hydrophilicity, and Young’s modulus values in the range of 1.40 to 7.50 MPa. In vitro assay on healthy human fibroblast (MRC5 cells) by MTT test and in vivo (Caenorhabditis elegans) survival assays show the advantageous biocompatible properties of novel IPN hydrogel scaffolds. Furthermore, in vitro controlled release study of the therapeutic agent resveratrol showed that these novel scaffolding systems are suitable controlled release platforms. The results revealed that the use of EDC and the combination of EDC/NHS crosslinkers can be applied to prepare and tune the properties of the IPN 2-hydroxyethyl methacrylate/alginate and 2-hydroxyethyl methacrylate/gelatin hydrogel scaffolds series, which have shown great potential for biomedical engineering applications.",
publisher = "MDPI",
journal = "Polymers",
title = "In Vitro and In Vivo Biocompatible and Controlled Resveratrol Release Performances of HEMA/Alginate and HEMA/Gelatin IPN Hydrogel Scaffolds",
number = "20",
pages = "4459",
volume = "14",
doi = "10.3390/polym14204459"
}

Vuković, J. S., Filipović, V. V., Babić Radić, M. M., Vukomanović, M., Milivojević, D., Ilić-Tomić, T., Nikodinović-Runić, J.,& Tomić, S. Lj.. (2022). In Vitro and In Vivo Biocompatible and Controlled Resveratrol Release Performances of HEMA/Alginate and HEMA/Gelatin IPN Hydrogel Scaffolds. in Polymers
MDPI., 14(20), 4459.
https://doi.org/10.3390/polym14204459

Vuković JS, Filipović VV, Babić Radić MM, Vukomanović M, Milivojević D, Ilić-Tomić T, Nikodinović-Runić J, Tomić SL. In Vitro and In Vivo Biocompatible and Controlled Resveratrol Release Performances of HEMA/Alginate and HEMA/Gelatin IPN Hydrogel Scaffolds. in Polymers. 2022;14(20):4459.
doi:10.3390/polym14204459 .

Vuković, Jovana S., Filipović, Vuk V., Babić Radić, Marija M., Vukomanović, Marija, Milivojević, Dušan, Ilić-Tomić, Tatjana, Nikodinović-Runić, Jasmina, Tomić, Simonida Lj., "In Vitro and In Vivo Biocompatible and Controlled Resveratrol Release Performances of HEMA/Alginate and HEMA/Gelatin IPN Hydrogel Scaffolds" in Polymers, 14, no. 20 (2022):4459,
https://doi.org/10.3390/polym14204459 . .

TY  - JOUR
AU  - Pekmezović, Marina
AU  - Kalagasidis Krušić, Melina
AU  - Malagurski, Ivana
AU  - Milovanović, Jelena
AU  - Stepien, Karolina
AU  - Guzik, Maciej
AU  - Charifou, Romina
AU  - Babu, Ramesh
AU  - O'Connor, Kevin
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4826
AB  - Novel biodegradable and biocompatible formulations of "old" but "gold" drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (similar to 50 mu m). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.
T2  - Antibiotics
T1  - Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency
IS  - 6
SP  - 737
VL  - 10
DO  - 10.3390/antibiotics10060737
ER  - 

@article{
author = "Pekmezović, Marina and Kalagasidis Krušić, Melina and Malagurski, Ivana and Milovanović, Jelena and Stepien, Karolina and Guzik, Maciej and Charifou, Romina and Babu, Ramesh and O'Connor, Kevin and Nikodinović-Runić, Jasmina",
year = "2021",
abstract = "Novel biodegradable and biocompatible formulations of "old" but "gold" drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (similar to 50 mu m). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.",
journal = "Antibiotics",
title = "Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency",
number = "6",
pages = "737",
volume = "10",
doi = "10.3390/antibiotics10060737"
}

Pekmezović, M., Kalagasidis Krušić, M., Malagurski, I., Milovanović, J., Stepien, K., Guzik, M., Charifou, R., Babu, R., O'Connor, K.,& Nikodinović-Runić, J.. (2021). Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency. in Antibiotics, 10(6), 737.
https://doi.org/10.3390/antibiotics10060737

Pekmezović M, Kalagasidis Krušić M, Malagurski I, Milovanović J, Stepien K, Guzik M, Charifou R, Babu R, O'Connor K, Nikodinović-Runić J. Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency. in Antibiotics. 2021;10(6):737.
doi:10.3390/antibiotics10060737 .

Pekmezović, Marina, Kalagasidis Krušić, Melina, Malagurski, Ivana, Milovanović, Jelena, Stepien, Karolina, Guzik, Maciej, Charifou, Romina, Babu, Ramesh, O'Connor, Kevin, Nikodinović-Runić, Jasmina, "Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency" in Antibiotics, 10, no. 6 (2021):737,
https://doi.org/10.3390/antibiotics10060737 . .

TY  - CONF
AU  - Kramar, Ana
AU  - Ilić-Tomić, Tatjana
AU  - Lađarević, Jelena
AU  - Nikodinović-Runić, Jasmina
AU  - Kostić, Mirjana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6755
AB  - In this work, cellulose (viscose) was functionalized with chitosan and dyed with an extract of Streptomyces sp. NP4 bacterial culture. Cellulose was first converted to dialdehyde cellulose; afterward chitosan was deposited, making fabric susceptible to dyeing. The dyed fabric exhibited pH responsiveness in a broad range of pH, 4-7-10, where colour shade of the fabric, exposed to buffer solutions, was pink, red, and blue, respectively. Response to the pH change was measured in seconds, whereby faster colour shift is from blue to red/pink. The dyed fabrics can withstand 10 cycles of colour change. Due to the broad range of pH activity, the presented cellulose product has the potential to be used as a textile pH sensor, and, more importantly, it is completely made from natural and sustainable resources.
PB  - Sciencentris
C3  - Book of Abstracts / 5th International International Conference on Natural Fibers - Materials of the Future, ICNF2021, 17–19. maj 2021., Portugal
T1  - Functional pH-sensitive cellulose fabric dyed with bacterial extract from Streptomyces sp.
EP  - 29
SP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6755
ER  - 

@conference{
author = "Kramar, Ana and Ilić-Tomić, Tatjana and Lađarević, Jelena and Nikodinović-Runić, Jasmina and Kostić, Mirjana",
year = "2021",
abstract = "In this work, cellulose (viscose) was functionalized with chitosan and dyed with an extract of Streptomyces sp. NP4 bacterial culture. Cellulose was first converted to dialdehyde cellulose; afterward chitosan was deposited, making fabric susceptible to dyeing. The dyed fabric exhibited pH responsiveness in a broad range of pH, 4-7-10, where colour shade of the fabric, exposed to buffer solutions, was pink, red, and blue, respectively. Response to the pH change was measured in seconds, whereby faster colour shift is from blue to red/pink. The dyed fabrics can withstand 10 cycles of colour change. Due to the broad range of pH activity, the presented cellulose product has the potential to be used as a textile pH sensor, and, more importantly, it is completely made from natural and sustainable resources.",
publisher = "Sciencentris",
journal = "Book of Abstracts / 5th International International Conference on Natural Fibers - Materials of the Future, ICNF2021, 17–19. maj 2021., Portugal",
title = "Functional pH-sensitive cellulose fabric dyed with bacterial extract from Streptomyces sp.",
pages = "29-28",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6755"
}

Kramar, A., Ilić-Tomić, T., Lađarević, J., Nikodinović-Runić, J.,& Kostić, M.. (2021). Functional pH-sensitive cellulose fabric dyed with bacterial extract from Streptomyces sp.. in Book of Abstracts / 5th International International Conference on Natural Fibers - Materials of the Future, ICNF2021, 17–19. maj 2021., Portugal
Sciencentris., 28-29.
https://hdl.handle.net/21.15107/rcub_technorep_6755

Kramar A, Ilić-Tomić T, Lađarević J, Nikodinović-Runić J, Kostić M. Functional pH-sensitive cellulose fabric dyed with bacterial extract from Streptomyces sp.. in Book of Abstracts / 5th International International Conference on Natural Fibers - Materials of the Future, ICNF2021, 17–19. maj 2021., Portugal. 2021;:28-29.
https://hdl.handle.net/21.15107/rcub_technorep_6755 .

Kramar, Ana, Ilić-Tomić, Tatjana, Lađarević, Jelena, Nikodinović-Runić, Jasmina, Kostić, Mirjana, "Functional pH-sensitive cellulose fabric dyed with bacterial extract from Streptomyces sp." in Book of Abstracts / 5th International International Conference on Natural Fibers - Materials of the Future, ICNF2021, 17–19. maj 2021., Portugal (2021):28-29,
https://hdl.handle.net/21.15107/rcub_technorep_6755 .

TY  - JOUR
AU  - Kramar, Ana
AU  - Ilic-Tomic, Tatjana R.
AU  - Lađarević, Jelena
AU  - Nikodinović-Runić, Jasmina
AU  - Kostić, Mirjana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4867
AB  - Halochromic (pH-responsive) material was obtained by dyeing functionalized viscose fabric with a crude extract from Streptomyces sp. strain NP4. The functionalization of the fabric before dyeing was performed to make cellulose susceptible to coloration with NP4 extract. Two combined pre-treatment steps were used, oxidation to obtain dialdehyde cellulose and chitosan deposition after oxidation. Chitosan was deposited onto untreated fabric as well, while only oxidized viscose was also investigated for dyeing. Functionalization by both protocols made viscose susceptible to dyeing with the notion that the deposition of chitosan onto oxidized viscose produced the darkest shade on the material. Dyed fabrics showed visual pH responsiveness in the range pH 4-10, with a color change from pink to red (pH 4-pH 7) and a major color change from red to blue (pH 7-pH 10) whereby fabric was tested and could withstand 10 color-changing cycles. Cytotoxicity assay confirmed the non-toxic nature of dyed material, which indicates its possible use as wound dressing's indicators.
T2  - Cellulose
T1  - Halochromic cellulose textile obtained via dyeing with biocolorant isolated from Streptomyces sp. strain NP4
EP  - 8784
IS  - 13
SP  - 8771
VL  - 28
DO  - 10.1007/s10570-021-04071-7
ER  - 

@article{
author = "Kramar, Ana and Ilic-Tomic, Tatjana R. and Lađarević, Jelena and Nikodinović-Runić, Jasmina and Kostić, Mirjana",
year = "2021",
abstract = "Halochromic (pH-responsive) material was obtained by dyeing functionalized viscose fabric with a crude extract from Streptomyces sp. strain NP4. The functionalization of the fabric before dyeing was performed to make cellulose susceptible to coloration with NP4 extract. Two combined pre-treatment steps were used, oxidation to obtain dialdehyde cellulose and chitosan deposition after oxidation. Chitosan was deposited onto untreated fabric as well, while only oxidized viscose was also investigated for dyeing. Functionalization by both protocols made viscose susceptible to dyeing with the notion that the deposition of chitosan onto oxidized viscose produced the darkest shade on the material. Dyed fabrics showed visual pH responsiveness in the range pH 4-10, with a color change from pink to red (pH 4-pH 7) and a major color change from red to blue (pH 7-pH 10) whereby fabric was tested and could withstand 10 color-changing cycles. Cytotoxicity assay confirmed the non-toxic nature of dyed material, which indicates its possible use as wound dressing's indicators.",
journal = "Cellulose",
title = "Halochromic cellulose textile obtained via dyeing with biocolorant isolated from Streptomyces sp. strain NP4",
pages = "8784-8771",
number = "13",
volume = "28",
doi = "10.1007/s10570-021-04071-7"
}

Kramar, A., Ilic-Tomic, T. R., Lađarević, J., Nikodinović-Runić, J.,& Kostić, M.. (2021). Halochromic cellulose textile obtained via dyeing with biocolorant isolated from Streptomyces sp. strain NP4. in Cellulose, 28(13), 8771-8784.
https://doi.org/10.1007/s10570-021-04071-7

Kramar A, Ilic-Tomic TR, Lađarević J, Nikodinović-Runić J, Kostić M. Halochromic cellulose textile obtained via dyeing with biocolorant isolated from Streptomyces sp. strain NP4. in Cellulose. 2021;28(13):8771-8784.
doi:10.1007/s10570-021-04071-7 .

Kramar, Ana, Ilic-Tomic, Tatjana R., Lađarević, Jelena, Nikodinović-Runić, Jasmina, Kostić, Mirjana, "Halochromic cellulose textile obtained via dyeing with biocolorant isolated from Streptomyces sp. strain NP4" in Cellulose, 28, no. 13 (2021):8771-8784,
https://doi.org/10.1007/s10570-021-04071-7 . .

TY  - JOUR
AU  - Stojkovska, Jasmina
AU  - Zvicer, Jovana
AU  - Milivojević, Milena
AU  - Petrović, Isidora
AU  - Stevanović, Milena
AU  - Obradović, Bojana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4373
AB  - Development of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100-250 mu m s(-1)) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in short term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Validation of a novel perfusion bioreactor system in cancer research
EP  - 196
IS  - 3
SP  - 187
VL  - 74
DO  - 10.2298/HEMIND200329015S
ER  - 

@article{
author = "Stojkovska, Jasmina and Zvicer, Jovana and Milivojević, Milena and Petrović, Isidora and Stevanović, Milena and Obradović, Bojana",
year = "2020",
abstract = "Development of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100-250 mu m s(-1)) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in short term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Validation of a novel perfusion bioreactor system in cancer research",
pages = "196-187",
number = "3",
volume = "74",
doi = "10.2298/HEMIND200329015S"
}

Stojkovska, J., Zvicer, J., Milivojević, M., Petrović, I., Stevanović, M.,& Obradović, B.. (2020). Validation of a novel perfusion bioreactor system in cancer research. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 74(3), 187-196.
https://doi.org/10.2298/HEMIND200329015S

Stojkovska J, Zvicer J, Milivojević M, Petrović I, Stevanović M, Obradović B. Validation of a novel perfusion bioreactor system in cancer research. in Hemijska industrija. 2020;74(3):187-196.
doi:10.2298/HEMIND200329015S .

Stojkovska, Jasmina, Zvicer, Jovana, Milivojević, Milena, Petrović, Isidora, Stevanović, Milena, Obradović, Bojana, "Validation of a novel perfusion bioreactor system in cancer research" in Hemijska industrija, 74, no. 3 (2020):187-196,
https://doi.org/10.2298/HEMIND200329015S . .

TY  - JOUR
AU  - Ponjavić, Marijana
AU  - Nikolić, Marija
AU  - Stevanović, Sanja
AU  - Nikodinović-Runić, Jasmina
AU  - Jeremić, Sanja
AU  - Pavić, Aleksandar
AU  - Đonlagić, Jasna
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4394
AB  - Star-shaped polymers of biodegradable aliphatic polyester, poly(epsilon-caprolactone), PCL, with different number of arms (three, four, and six) were synthesized by ring-opening polymerization initiated by multifunctional alcohols used as cores. As potential biomaterials, synthesized star-shaped poly(epsilon-caprolactone)s,sPCL, were thoroughly characterized in terms of their degradation under different pH conditions and in respect to their cytotoxicity. The in vitro degradation was performed in phosphate buffer (pH 7.4) and hydrochloric acid solution (pH 1.0) over 5 weeks. Degradation ofsPCL films was followed by the weight loss measurements, GPC, FTIR, and AFM analysis. While the most of the samples were stable against the abiotic hydrolysis at pH 7.4 after 5 weeks of degradation, degradation was significantly accelerated in the acidic medium. Degradation rate of polymer films was affected by the polymer architecture and molecular weight. The molecular weight profiles during the degradation revealed random chain scission of the ester bonds indicating bulk degradation mechanism of hydrolysis at pH 7.4, while acidic hydrolysis proceeded through the bulk degradation associated with surface erosion, confirmed by AFM. The in vitro toxicity tests, cytotoxicity applying normal human fibroblasts (MRC5) and embryotoxicity assessment (using zebra fish model,Danio rerio), suggested those polymeric materials as suitable for biomedical application.
PB  - Sage Publications Ltd, London
T2  - Journal of Bioactive and Compatible Polymers
T1  - Hydrolytic degradation of star-shaped poly(epsilon-caprolactone)s with different number of arms and their cytotoxic effects
EP  - 537
IS  - 6
SP  - 517
VL  - 35
DO  - 10.1177/0883911520951826
ER  - 

@article{
author = "Ponjavić, Marijana and Nikolić, Marija and Stevanović, Sanja and Nikodinović-Runić, Jasmina and Jeremić, Sanja and Pavić, Aleksandar and Đonlagić, Jasna",
year = "2020",
abstract = "Star-shaped polymers of biodegradable aliphatic polyester, poly(epsilon-caprolactone), PCL, with different number of arms (three, four, and six) were synthesized by ring-opening polymerization initiated by multifunctional alcohols used as cores. As potential biomaterials, synthesized star-shaped poly(epsilon-caprolactone)s,sPCL, were thoroughly characterized in terms of their degradation under different pH conditions and in respect to their cytotoxicity. The in vitro degradation was performed in phosphate buffer (pH 7.4) and hydrochloric acid solution (pH 1.0) over 5 weeks. Degradation ofsPCL films was followed by the weight loss measurements, GPC, FTIR, and AFM analysis. While the most of the samples were stable against the abiotic hydrolysis at pH 7.4 after 5 weeks of degradation, degradation was significantly accelerated in the acidic medium. Degradation rate of polymer films was affected by the polymer architecture and molecular weight. The molecular weight profiles during the degradation revealed random chain scission of the ester bonds indicating bulk degradation mechanism of hydrolysis at pH 7.4, while acidic hydrolysis proceeded through the bulk degradation associated with surface erosion, confirmed by AFM. The in vitro toxicity tests, cytotoxicity applying normal human fibroblasts (MRC5) and embryotoxicity assessment (using zebra fish model,Danio rerio), suggested those polymeric materials as suitable for biomedical application.",
publisher = "Sage Publications Ltd, London",
journal = "Journal of Bioactive and Compatible Polymers",
title = "Hydrolytic degradation of star-shaped poly(epsilon-caprolactone)s with different number of arms and their cytotoxic effects",
pages = "537-517",
number = "6",
volume = "35",
doi = "10.1177/0883911520951826"
}

Ponjavić, M., Nikolić, M., Stevanović, S., Nikodinović-Runić, J., Jeremić, S., Pavić, A.,& Đonlagić, J.. (2020). Hydrolytic degradation of star-shaped poly(epsilon-caprolactone)s with different number of arms and their cytotoxic effects. in Journal of Bioactive and Compatible Polymers
Sage Publications Ltd, London., 35(6), 517-537.
https://doi.org/10.1177/0883911520951826

Ponjavić M, Nikolić M, Stevanović S, Nikodinović-Runić J, Jeremić S, Pavić A, Đonlagić J. Hydrolytic degradation of star-shaped poly(epsilon-caprolactone)s with different number of arms and their cytotoxic effects. in Journal of Bioactive and Compatible Polymers. 2020;35(6):517-537.
doi:10.1177/0883911520951826 .

Ponjavić, Marijana, Nikolić, Marija, Stevanović, Sanja, Nikodinović-Runić, Jasmina, Jeremić, Sanja, Pavić, Aleksandar, Đonlagić, Jasna, "Hydrolytic degradation of star-shaped poly(epsilon-caprolactone)s with different number of arms and their cytotoxic effects" in Journal of Bioactive and Compatible Polymers, 35, no. 6 (2020):517-537,
https://doi.org/10.1177/0883911520951826 . .