Basic Science Research Program of the Ministry of Education, Science and Technology of Korea [2018R1A2B5A02023190]

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Basic Science Research Program of the Ministry of Education, Science and Technology of Korea [2018R1A2B5A02023190]

Authors

Publications

Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering

Stevanović, Milena; Đošić, Marija; Janković, Ana; Kojić, Vesna; Vukašinović-Sekulić, Maja; Stojanović, Jovica; Odović, Jadranka; Crevar-Sakač, Milkica; Kyong Yop, Rhee; Mišković-Stanković, Vesna

(Wiley, Hoboken, 2020)

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakač, Milkica
AU  - Kyong Yop, Rhee
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4501
AB  - Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect againstStaphylococcus aureus(bactericidal, viable cells number reduction  gt 3 logarithmic units) compared toEscherichia coli(bacteriostatic, lt 3 logarithmic units).MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.
PB  - Wiley, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering
EP  - 2189
IS  - 11
SP  - 2175
VL  - 108
DO  - 10.1002/jbm.a.36974
ER  - 
@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakač, Milkica and Kyong Yop, Rhee and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect againstStaphylococcus aureus(bactericidal, viable cells number reduction  gt 3 logarithmic units) compared toEscherichia coli(bacteriostatic, lt 3 logarithmic units).MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.",
publisher = "Wiley, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering",
pages = "2189-2175",
number = "11",
volume = "108",
doi = "10.1002/jbm.a.36974"
}
Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukašinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakač, M., Kyong Yop, R.,& Mišković-Stanković, V.. (2020). Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research Part A
Wiley, Hoboken., 108(11), 2175-2189.
https://doi.org/10.1002/jbm.a.36974
Stevanović M, Đošić M, Janković A, Kojić V, Vukašinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakač M, Kyong Yop R, Mišković-Stanković V. Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research Part A. 2020;108(11):2175-2189.
doi:10.1002/jbm.a.36974 .
Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakač, Milkica, Kyong Yop, Rhee, Mišković-Stanković, Vesna, "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering" in Journal of Biomedical Materials Research Part A, 108, no. 11 (2020):2175-2189,
https://doi.org/10.1002/jbm.a.36974 . .
43
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41

Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering

Stevanović, Milena; Đošić, Marija; Janković, Ana; Kojić, Vesna; Vukašinović-Sekulić, Maja; Stojanović, Jovica; Odović, Jadranka; Crevar-Sakač, Milkica; Kyong Yop, Rhee; Mišković-Stanković, Vesna

(Wiley, Hoboken, 2020)

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakač, Milkica
AU  - Kyong Yop, Rhee
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4725
AB  - Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect againstStaphylococcus aureus(bactericidal, viable cells number reduction  gt 3 logarithmic units) compared toEscherichia coli(bacteriostatic, lt 3 logarithmic units).MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.
PB  - Wiley, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering
EP  - 2189
IS  - 11
SP  - 2175
VL  - 108
DO  - 10.1002/jbm.a.36974
ER  - 
@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakač, Milkica and Kyong Yop, Rhee and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect againstStaphylococcus aureus(bactericidal, viable cells number reduction  gt 3 logarithmic units) compared toEscherichia coli(bacteriostatic, lt 3 logarithmic units).MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.",
publisher = "Wiley, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering",
pages = "2189-2175",
number = "11",
volume = "108",
doi = "10.1002/jbm.a.36974"
}
Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukašinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakač, M., Kyong Yop, R.,& Mišković-Stanković, V.. (2020). Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research Part A
Wiley, Hoboken., 108(11), 2175-2189.
https://doi.org/10.1002/jbm.a.36974
Stevanović M, Đošić M, Janković A, Kojić V, Vukašinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakač M, Kyong Yop R, Mišković-Stanković V. Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research Part A. 2020;108(11):2175-2189.
doi:10.1002/jbm.a.36974 .
Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakač, Milkica, Kyong Yop, Rhee, Mišković-Stanković, Vesna, "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering" in Journal of Biomedical Materials Research Part A, 108, no. 11 (2020):2175-2189,
https://doi.org/10.1002/jbm.a.36974 . .
43
14
44

Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate

Stevanović, Milena; Đošić, Marija; Janković, Ana; Nešović, Katarina; Kojić, Vesna; Stojanović, Jovica; Grujić, Svetlana; Matić-Bujagić, Ivana; Rhee, Kyong Yop; Mišković-Stanković, Vesna

(Amer Chemical Soc, Washington, 2020)

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Nešović, Katarina
AU  - Kojić, Vesna
AU  - Stojanović, Jovica
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4498
AB  - The electrophoretic deposition process (EPD) was utilized to produce bioactive hydroxyapatite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) coatings on titanium. The bioactivity of newly synthesized composite coatings was investigated in the simulated body fluid (SBF) and examined by X-ray diffraction, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy. The obtained results revealed carbonate-substituted hydroxyapatite after immersion in SBF, emphasizing the similarity of the biomimetically grown HAP with the naturally occurring apatite in the bone. The formation of biomimetic HAP was confirmed by electrochemical impedance spectroscopy and polarization measurements, through the decrease in corrosion current density and coating capacitance values after 28-day immersion in SBF. The osseointegration ability was further validated by measuring the alkaline phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings (significant increase in ALP levels for both HAP/CS (3.206 U mL(-1)) and HAP/CS/Gent (4.039 U mL(-1)) coatings, compared to the control (0.900 U mL(-1))). Drug-release kinetics was investigated in deionized water at 37 degrees C by high-performance liquid chromatography coupled with mass spectrometry. Release profiles revealed the beneficial "burst-release effect" (similar to 21% of gentamicin released in the first 48 h) as a potentially promising solution against the biofilm formation in the initial period. When tested against human and mice fibroblast cells (MRC-5 and L929), both composite coatings showed a noncytotoxic effect (viability  gt 85%), providing a promising basis for further medical application trials.
PB  - Amer Chemical Soc, Washington
T2  - ACS Omega
T1  - Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate
EP  - 15445
IS  - 25
SP  - 15433
VL  - 5
DO  - 10.1021/acsomega.0c01583
ER  - 
@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Nešović, Katarina and Kojić, Vesna and Stojanović, Jovica and Grujić, Svetlana and Matić-Bujagić, Ivana and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2020",
abstract = "The electrophoretic deposition process (EPD) was utilized to produce bioactive hydroxyapatite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) coatings on titanium. The bioactivity of newly synthesized composite coatings was investigated in the simulated body fluid (SBF) and examined by X-ray diffraction, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy. The obtained results revealed carbonate-substituted hydroxyapatite after immersion in SBF, emphasizing the similarity of the biomimetically grown HAP with the naturally occurring apatite in the bone. The formation of biomimetic HAP was confirmed by electrochemical impedance spectroscopy and polarization measurements, through the decrease in corrosion current density and coating capacitance values after 28-day immersion in SBF. The osseointegration ability was further validated by measuring the alkaline phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings (significant increase in ALP levels for both HAP/CS (3.206 U mL(-1)) and HAP/CS/Gent (4.039 U mL(-1)) coatings, compared to the control (0.900 U mL(-1))). Drug-release kinetics was investigated in deionized water at 37 degrees C by high-performance liquid chromatography coupled with mass spectrometry. Release profiles revealed the beneficial "burst-release effect" (similar to 21% of gentamicin released in the first 48 h) as a potentially promising solution against the biofilm formation in the initial period. When tested against human and mice fibroblast cells (MRC-5 and L929), both composite coatings showed a noncytotoxic effect (viability  gt 85%), providing a promising basis for further medical application trials.",
publisher = "Amer Chemical Soc, Washington",
journal = "ACS Omega",
title = "Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate",
pages = "15445-15433",
number = "25",
volume = "5",
doi = "10.1021/acsomega.0c01583"
}
Stevanović, M., Đošić, M., Janković, A., Nešović, K., Kojić, V., Stojanović, J., Grujić, S., Matić-Bujagić, I., Rhee, K. Y.,& Mišković-Stanković, V.. (2020). Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate. in ACS Omega
Amer Chemical Soc, Washington., 5(25), 15433-15445.
https://doi.org/10.1021/acsomega.0c01583
Stevanović M, Đošić M, Janković A, Nešović K, Kojić V, Stojanović J, Grujić S, Matić-Bujagić I, Rhee KY, Mišković-Stanković V. Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate. in ACS Omega. 2020;5(25):15433-15445.
doi:10.1021/acsomega.0c01583 .
Stevanović, Milena, Đošić, Marija, Janković, Ana, Nešović, Katarina, Kojić, Vesna, Stojanović, Jovica, Grujić, Svetlana, Matić-Bujagić, Ivana, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate" in ACS Omega, 5, no. 25 (2020):15433-15445,
https://doi.org/10.1021/acsomega.0c01583 . .
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29

Kinetic models of swelling and thermal stability of silver/poly(vinyl Check far alcohol)/chitosan/graphene hydrogels

Nešović, Katarina; Janković, Ana; Perić-Grujić, Aleksandra; Vukašinović-Sekulić, Maja; Radetić, Tamara; Živković, Ljiljana; Park, Soo Jin; Rhee, Kyong Yop; Mišković-Stanković, Vesna

(Elsevier Science Inc, New York, 2019)

TY  - JOUR
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Perić-Grujić, Aleksandra
AU  - Vukašinović-Sekulić, Maja
AU  - Radetić, Tamara
AU  - Živković, Ljiljana
AU  - Park, Soo Jin
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4239
AB  - Silver nanoparticles (AgNPs) were synthesized by in situ electrochemical reduction of Ag+ ions in poly (vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) hydrogel matrices with different concentrations of chitosan. The physicochemical properties of nanocomposite hydrogels were investigated by UV-vis spectroscopy (UV-vis), dynamic light scattering (DLS), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FT-IR), thermal characteristics were determined by differential scanning calorimetry (DSC) and mechanical properties were measured by tensile test. The swelling studies were carried out in phosphate buffer to simulate natural physiological environment and data were fitted by several kinetic models to determine the diffusion mechanism and diffusion coefficients of the swelling medium through the hydrogel matrices. It was shown that the presence of silver nanoparticles increased the uptake capability and equilibrium swelling degree of the composite hydrogels. The antibacterial activity was confirmed against Escherichia coli and Staphylococcus aureus, while the hydrogels without AgNPs exhibited antibacterial properties due to the presence of chitosan. With the addition of AgNPs, the samples showed stronger activity and fast reduction in the number of colonies, confirming the synergistic effect of chitosan and AgNPs on the antibacterial activity.
PB  - Elsevier Science Inc, New York
T2  - Journal of Industrial and Engineering Chemistry
T1  - Kinetic models of swelling and thermal stability of silver/poly(vinyl Check far alcohol)/chitosan/graphene hydrogels
EP  - 96
SP  - 83
VL  - 77
DO  - 10.1016/j.jiec.2019.04.022
ER  - 
@article{
author = "Nešović, Katarina and Janković, Ana and Perić-Grujić, Aleksandra and Vukašinović-Sekulić, Maja and Radetić, Tamara and Živković, Ljiljana and Park, Soo Jin and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2019",
abstract = "Silver nanoparticles (AgNPs) were synthesized by in situ electrochemical reduction of Ag+ ions in poly (vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) hydrogel matrices with different concentrations of chitosan. The physicochemical properties of nanocomposite hydrogels were investigated by UV-vis spectroscopy (UV-vis), dynamic light scattering (DLS), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FT-IR), thermal characteristics were determined by differential scanning calorimetry (DSC) and mechanical properties were measured by tensile test. The swelling studies were carried out in phosphate buffer to simulate natural physiological environment and data were fitted by several kinetic models to determine the diffusion mechanism and diffusion coefficients of the swelling medium through the hydrogel matrices. It was shown that the presence of silver nanoparticles increased the uptake capability and equilibrium swelling degree of the composite hydrogels. The antibacterial activity was confirmed against Escherichia coli and Staphylococcus aureus, while the hydrogels without AgNPs exhibited antibacterial properties due to the presence of chitosan. With the addition of AgNPs, the samples showed stronger activity and fast reduction in the number of colonies, confirming the synergistic effect of chitosan and AgNPs on the antibacterial activity.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Industrial and Engineering Chemistry",
title = "Kinetic models of swelling and thermal stability of silver/poly(vinyl Check far alcohol)/chitosan/graphene hydrogels",
pages = "96-83",
volume = "77",
doi = "10.1016/j.jiec.2019.04.022"
}
Nešović, K., Janković, A., Perić-Grujić, A., Vukašinović-Sekulić, M., Radetić, T., Živković, L., Park, S. J., Rhee, K. Y.,& Mišković-Stanković, V.. (2019). Kinetic models of swelling and thermal stability of silver/poly(vinyl Check far alcohol)/chitosan/graphene hydrogels. in Journal of Industrial and Engineering Chemistry
Elsevier Science Inc, New York., 77, 83-96.
https://doi.org/10.1016/j.jiec.2019.04.022
Nešović K, Janković A, Perić-Grujić A, Vukašinović-Sekulić M, Radetić T, Živković L, Park SJ, Rhee KY, Mišković-Stanković V. Kinetic models of swelling and thermal stability of silver/poly(vinyl Check far alcohol)/chitosan/graphene hydrogels. in Journal of Industrial and Engineering Chemistry. 2019;77:83-96.
doi:10.1016/j.jiec.2019.04.022 .
Nešović, Katarina, Janković, Ana, Perić-Grujić, Aleksandra, Vukašinović-Sekulić, Maja, Radetić, Tamara, Živković, Ljiljana, Park, Soo Jin, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Kinetic models of swelling and thermal stability of silver/poly(vinyl Check far alcohol)/chitosan/graphene hydrogels" in Journal of Industrial and Engineering Chemistry, 77 (2019):83-96,
https://doi.org/10.1016/j.jiec.2019.04.022 . .
25
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27

The effect of ceria and zirconia nanoparticles on the corrosion behaviour of cataphoretic epoxy coatings on AA6060 alloy

Živković, Ljiljana; Jegdić, Bore; Andrić, Velibor; Rhee, Kyong Yop; Bajat, Jelena; Mišković-Stanković, Vesna

(Elsevier Science Sa, Lausanne, 2019)

TY  - JOUR
AU  - Živković, Ljiljana
AU  - Jegdić, Bore
AU  - Andrić, Velibor
AU  - Rhee, Kyong Yop
AU  - Bajat, Jelena
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4046
AB  - In this work an attempt was made to produce epoxy coating doped with ceria (CeO2) and/or zirconia (ZrO2) nanoparticles, in single step electrophoretric deposition process. Deposition was carried out on AA6060 cataforetically from epoxy resin suspension containing synthesized stable aqueous colloidal dispersions (sols) of CeO2 and ZrO2. The influence of ceria and zirconia nanoparticles on the physicochemical characteristics of epoxy coatings was evaluated by energy dispersive X-ray fluorescence spectroscopy, X-ray photoelectron spectroscopy, field-emission scanning electron microscopy, Fourier-transform infrared spectroscopy, thermogravimetric analysis and adhesion measurements, while their corrosion stability during immersion in 0.5 M NaCl solution was monitored by electrochemical impedance spectroscopy. All ceria and/or zirconia containing coatings showed better corrosion stability and adhesion than pure epoxy coating, while between doped epoxy coatings one with CeO2/ZrO2 exhhibited the greatest values of charge-transfer resistance due to synergetic effect of both types of added nanoparticles.
PB  - Elsevier Science Sa, Lausanne
T2  - Progress in Organic Coatings
T1  - The effect of ceria and zirconia nanoparticles on the corrosion behaviour of cataphoretic epoxy coatings on AA6060 alloy
VL  - 136
DO  - 10.1016/j.porgcoat.2019.105219
ER  - 
@article{
author = "Živković, Ljiljana and Jegdić, Bore and Andrić, Velibor and Rhee, Kyong Yop and Bajat, Jelena and Mišković-Stanković, Vesna",
year = "2019",
abstract = "In this work an attempt was made to produce epoxy coating doped with ceria (CeO2) and/or zirconia (ZrO2) nanoparticles, in single step electrophoretric deposition process. Deposition was carried out on AA6060 cataforetically from epoxy resin suspension containing synthesized stable aqueous colloidal dispersions (sols) of CeO2 and ZrO2. The influence of ceria and zirconia nanoparticles on the physicochemical characteristics of epoxy coatings was evaluated by energy dispersive X-ray fluorescence spectroscopy, X-ray photoelectron spectroscopy, field-emission scanning electron microscopy, Fourier-transform infrared spectroscopy, thermogravimetric analysis and adhesion measurements, while their corrosion stability during immersion in 0.5 M NaCl solution was monitored by electrochemical impedance spectroscopy. All ceria and/or zirconia containing coatings showed better corrosion stability and adhesion than pure epoxy coating, while between doped epoxy coatings one with CeO2/ZrO2 exhhibited the greatest values of charge-transfer resistance due to synergetic effect of both types of added nanoparticles.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Progress in Organic Coatings",
title = "The effect of ceria and zirconia nanoparticles on the corrosion behaviour of cataphoretic epoxy coatings on AA6060 alloy",
volume = "136",
doi = "10.1016/j.porgcoat.2019.105219"
}
Živković, L., Jegdić, B., Andrić, V., Rhee, K. Y., Bajat, J.,& Mišković-Stanković, V.. (2019). The effect of ceria and zirconia nanoparticles on the corrosion behaviour of cataphoretic epoxy coatings on AA6060 alloy. in Progress in Organic Coatings
Elsevier Science Sa, Lausanne., 136.
https://doi.org/10.1016/j.porgcoat.2019.105219
Živković L, Jegdić B, Andrić V, Rhee KY, Bajat J, Mišković-Stanković V. The effect of ceria and zirconia nanoparticles on the corrosion behaviour of cataphoretic epoxy coatings on AA6060 alloy. in Progress in Organic Coatings. 2019;136.
doi:10.1016/j.porgcoat.2019.105219 .
Živković, Ljiljana, Jegdić, Bore, Andrić, Velibor, Rhee, Kyong Yop, Bajat, Jelena, Mišković-Stanković, Vesna, "The effect of ceria and zirconia nanoparticles on the corrosion behaviour of cataphoretic epoxy coatings on AA6060 alloy" in Progress in Organic Coatings, 136 (2019),
https://doi.org/10.1016/j.porgcoat.2019.105219 . .
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Investigation of corrosion behaviour of carbon nanotubes coated basalt fabric as a reinforcement material

Mittal, Garima; Nešović, Katarina; Rhee, Kyong Yop; Mišković-Stanković, Vesna

(Elsevier Sci Ltd, Oxford, 2019)

TY  - JOUR
AU  - Mittal, Garima
AU  - Nešović, Katarina
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4134
AB  - In this study, the corrosion behaviour of carbon nanotubes (CNTs) coated basalt fibre (BF) (BFCNTs) obtained by chemical vapour deposition (CVD), was studied. During CVD, the CNTs were grown on BF, (a) at different growth temperatures and (b) for different growth times. The physicochemical measurements were performed using X-ray diffraction, Raman spectroscopy, and field-emission scanning electron microscopy. The corrosion performances were investigated using electrochemical impedance spectroscopy (EIS), potentiodynamic sweep (PDS), linear polarization resistance (LPR) and weight loss measurements. The BFCNT prepared at 650 degrees C for 120 min exhibited the best corrosion stability during the prolonged exposure to 0.1 M NaCl electrolyte. The outcomes of this study would be useful in designing the carbon nanotubes coated basalt fibre with the best corrosion resistance.
PB  - Elsevier Sci Ltd, Oxford
T2  - Composites Part B-Engineering
T1  - Investigation of corrosion behaviour of carbon nanotubes coated basalt fabric as a reinforcement material
VL  - 178
DO  - 10.1016/j.compositesb.2019.107493
ER  - 
@article{
author = "Mittal, Garima and Nešović, Katarina and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2019",
abstract = "In this study, the corrosion behaviour of carbon nanotubes (CNTs) coated basalt fibre (BF) (BFCNTs) obtained by chemical vapour deposition (CVD), was studied. During CVD, the CNTs were grown on BF, (a) at different growth temperatures and (b) for different growth times. The physicochemical measurements were performed using X-ray diffraction, Raman spectroscopy, and field-emission scanning electron microscopy. The corrosion performances were investigated using electrochemical impedance spectroscopy (EIS), potentiodynamic sweep (PDS), linear polarization resistance (LPR) and weight loss measurements. The BFCNT prepared at 650 degrees C for 120 min exhibited the best corrosion stability during the prolonged exposure to 0.1 M NaCl electrolyte. The outcomes of this study would be useful in designing the carbon nanotubes coated basalt fibre with the best corrosion resistance.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Composites Part B-Engineering",
title = "Investigation of corrosion behaviour of carbon nanotubes coated basalt fabric as a reinforcement material",
volume = "178",
doi = "10.1016/j.compositesb.2019.107493"
}
Mittal, G., Nešović, K., Rhee, K. Y.,& Mišković-Stanković, V.. (2019). Investigation of corrosion behaviour of carbon nanotubes coated basalt fabric as a reinforcement material. in Composites Part B-Engineering
Elsevier Sci Ltd, Oxford., 178.
https://doi.org/10.1016/j.compositesb.2019.107493
Mittal G, Nešović K, Rhee KY, Mišković-Stanković V. Investigation of corrosion behaviour of carbon nanotubes coated basalt fabric as a reinforcement material. in Composites Part B-Engineering. 2019;178.
doi:10.1016/j.compositesb.2019.107493 .
Mittal, Garima, Nešović, Katarina, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Investigation of corrosion behaviour of carbon nanotubes coated basalt fabric as a reinforcement material" in Composites Part B-Engineering, 178 (2019),
https://doi.org/10.1016/j.compositesb.2019.107493 . .
24
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23

Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium

Stevanović, Milena; Đošić, Marija; Janković, Ana; Kojić, Vesna; Vukašinović-Sekulić, Maja; Stojanović, Jovica; Odović, Jadranka; Crevar-Sakač, Milkica; Rhee, Kyong Yop; Mišković-Stanković, Vesna

(Amer Chemical Soc, Washington, 2018)

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakač, Milkica
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3899
AB  - Composite coating of antibiotic gentamicin (Gent), natural polymer chitosan (CS), and hydroxyapatite (HAP) was successfully assessed by applying the electrophoretic deposition (EPD) technique. EPD was performed under optimized deposition conditions (5 V, 12 min) on pure titanium plates, to obtain HAP/CS and HAP/CS/Gent composite coatings in a single step from three-component aqueous suspension, with favorable antibacterial properties. Composite coatings were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray photoelectron analysis, confirming the formation of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface, which is due to intermolecular hydrogen bonds. Employing the XRD technique, HAP was detected by obtaining the characteristic diffraction maximums. Good antibacterial activity of the composite coating loaded with antibiotic (HAP/CS/Gent) was confirmed against Staphylococcus aureus and Escherichia coli, pointing to the high potential for bioapplication. Introduction of gentamicin in HAP/CS/Gent coating caused very mild cytotoxicity in the tested cell lines MRC-5 and L929. MTT testing was used to evaluate cell viability, and HAP/CS was classified as noncytotoxic.
PB  - Amer Chemical Soc, Washington
T2  - ACS Biomaterials Science & Engineering
T1  - Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium
EP  - 4007
IS  - 12
SP  - 3994
VL  - 4
DO  - 10.1021/acsbiomaterials.8b00859
ER  - 
@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakač, Milkica and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2018",
abstract = "Composite coating of antibiotic gentamicin (Gent), natural polymer chitosan (CS), and hydroxyapatite (HAP) was successfully assessed by applying the electrophoretic deposition (EPD) technique. EPD was performed under optimized deposition conditions (5 V, 12 min) on pure titanium plates, to obtain HAP/CS and HAP/CS/Gent composite coatings in a single step from three-component aqueous suspension, with favorable antibacterial properties. Composite coatings were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray photoelectron analysis, confirming the formation of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface, which is due to intermolecular hydrogen bonds. Employing the XRD technique, HAP was detected by obtaining the characteristic diffraction maximums. Good antibacterial activity of the composite coating loaded with antibiotic (HAP/CS/Gent) was confirmed against Staphylococcus aureus and Escherichia coli, pointing to the high potential for bioapplication. Introduction of gentamicin in HAP/CS/Gent coating caused very mild cytotoxicity in the tested cell lines MRC-5 and L929. MTT testing was used to evaluate cell viability, and HAP/CS was classified as noncytotoxic.",
publisher = "Amer Chemical Soc, Washington",
journal = "ACS Biomaterials Science & Engineering",
title = "Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium",
pages = "4007-3994",
number = "12",
volume = "4",
doi = "10.1021/acsbiomaterials.8b00859"
}
Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukašinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakač, M., Rhee, K. Y.,& Mišković-Stanković, V.. (2018). Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium. in ACS Biomaterials Science & Engineering
Amer Chemical Soc, Washington., 4(12), 3994-4007.
https://doi.org/10.1021/acsbiomaterials.8b00859
Stevanović M, Đošić M, Janković A, Kojić V, Vukašinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakač M, Rhee KY, Mišković-Stanković V. Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium. in ACS Biomaterials Science & Engineering. 2018;4(12):3994-4007.
doi:10.1021/acsbiomaterials.8b00859 .
Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakač, Milkica, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium" in ACS Biomaterials Science & Engineering, 4, no. 12 (2018):3994-4007,
https://doi.org/10.1021/acsbiomaterials.8b00859 . .
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Silver/poly(vinyl alcohol)/chitosan/graphene hydrogels - Synthesis, biological and physicochemical properties and silver release kinetics

Nešović, Katarina; Janković, Ana; Kojić, Vesna; Vukašinović-Sekulić, Maja; Perić-Grujić, Aleksandra; Rhee, Kyong Yop; Mišković-Stanković, Vesna

(Elsevier Sci Ltd, Oxford, 2018)

TY  - JOUR
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Perić-Grujić, Aleksandra
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3942
AB  - This study presents the synthesis of novel silver/poly(vinyl alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) nanocomposite hydrogels by in situ electrochemical reduction of silver ions in the hydrogel matrix and their thorough characterization by UV-visible and Raman spectroscopies, field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), and silver release measurements. The influence of chitosan content on the incorporation and stabilization of silver nanoparticles (AgNPs) was investigated, and we found that hydrogels with higher chitosan content contain higher amounts of AgNPs. In addition, the cytotoxicity and antibacterial activity of Ag/PVA/CHI/Gr nanocomposite hydrogels were evaluated. Based on in vitro investigations, the obtained materials exhibit diffusion-controlled release profiles over 28 days, strong antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains, and no cytotoxicity toward human and mice fibroblast cell lines.
PB  - Elsevier Sci Ltd, Oxford
T2  - Composites Part B-Engineering
T1  - Silver/poly(vinyl alcohol)/chitosan/graphene hydrogels - Synthesis, biological and physicochemical properties and silver release kinetics
EP  - 185
SP  - 175
VL  - 154
DO  - 10.1016/j.compositesb.2018.08.005
ER  - 
@article{
author = "Nešović, Katarina and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Perić-Grujić, Aleksandra and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2018",
abstract = "This study presents the synthesis of novel silver/poly(vinyl alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) nanocomposite hydrogels by in situ electrochemical reduction of silver ions in the hydrogel matrix and their thorough characterization by UV-visible and Raman spectroscopies, field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), and silver release measurements. The influence of chitosan content on the incorporation and stabilization of silver nanoparticles (AgNPs) was investigated, and we found that hydrogels with higher chitosan content contain higher amounts of AgNPs. In addition, the cytotoxicity and antibacterial activity of Ag/PVA/CHI/Gr nanocomposite hydrogels were evaluated. Based on in vitro investigations, the obtained materials exhibit diffusion-controlled release profiles over 28 days, strong antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains, and no cytotoxicity toward human and mice fibroblast cell lines.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Composites Part B-Engineering",
title = "Silver/poly(vinyl alcohol)/chitosan/graphene hydrogels - Synthesis, biological and physicochemical properties and silver release kinetics",
pages = "185-175",
volume = "154",
doi = "10.1016/j.compositesb.2018.08.005"
}
Nešović, K., Janković, A., Kojić, V., Vukašinović-Sekulić, M., Perić-Grujić, A., Rhee, K. Y.,& Mišković-Stanković, V.. (2018). Silver/poly(vinyl alcohol)/chitosan/graphene hydrogels - Synthesis, biological and physicochemical properties and silver release kinetics. in Composites Part B-Engineering
Elsevier Sci Ltd, Oxford., 154, 175-185.
https://doi.org/10.1016/j.compositesb.2018.08.005
Nešović K, Janković A, Kojić V, Vukašinović-Sekulić M, Perić-Grujić A, Rhee KY, Mišković-Stanković V. Silver/poly(vinyl alcohol)/chitosan/graphene hydrogels - Synthesis, biological and physicochemical properties and silver release kinetics. in Composites Part B-Engineering. 2018;154:175-185.
doi:10.1016/j.compositesb.2018.08.005 .
Nešović, Katarina, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Perić-Grujić, Aleksandra, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Silver/poly(vinyl alcohol)/chitosan/graphene hydrogels - Synthesis, biological and physicochemical properties and silver release kinetics" in Composites Part B-Engineering, 154 (2018):175-185,
https://doi.org/10.1016/j.compositesb.2018.08.005 . .
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