@article{
author = "Stojkovska, Jasmina and Bugarski, Branko and Obradović, Bojana",
year = "2010",
abstract = "Alginate hydrogels in forms of discs and packed beds of microbeads (similar to 800 mu m) were tested in a novel bioreactor at 10% strain using two regimes: at a loading rate of 337.5 mu m/s and at sequential increments of 50 mu m displacement every 30 min. Compressive strength increased with the increase in alginate concentration (1.5 vs. 2% w/w) and the content of guluronic residues (38.5 vs. 67%). Packed beds of microbeads exhibited significantly higher (similar to 1.5-3.4 fold) compression moduli than the respective discs indicating the effects of gel form and entrapped water. Short-term cultivation of microbeads with immobilized bovine calf chondrocytes (1.5% w/w, 33 x 10(6) cells/ml) under biomimetic conditions (dynamic compression: 1 h on/1 h off, 0.42 Hz, 10% strain) resulted in cell proliferation and bed compaction, so that the compression modulus slightly increased. Thus, the novel bioreactor demonstrated advantages in evaluation of biomaterial properties and cell-biomaterial interactions under in vivo-like settings.",
publisher = "Springer, Dordrecht",
journal = "Journal of Materials Science-Materials in Medicine",
title = "Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering",
pages = "2879-2869",
number = "10",
volume = "21",
doi = "10.1007/s10856-010-4135-0"
}