@article{
author = "Ilić, Nevena and Davidović, Slađana and Milić, Marija and Lađarević, Jelena and Onjia, Antonije and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina",
year = "2024",
abstract = "This study presents an innovative approach for the reuse and recycling of waste material, brewer’s spent grain (BSG) for
creating a novel green biocatalyst. The same BSG was utilized in several consecutive steps: initially, it served as a substrate
for the cultivation and production of laccase by a novel isolated fungal strain, Coriolopsis trogii 2SMKN, then, it was reused
as a carrier for laccase immobilization, aiding in the process of azo dye decolorization and fnally, reused as recycled BSG
for the second successful laccase immobilization for six guaiacol oxidation, contributing to a zero-waste strategy. The novel
fungal strain produced laccase with a maximum activity of 171.4 U/g after 6 days of solid-state fermentation using BSG
as a substrate. The obtained laccase exhibited excellent performance in the decolorization of azo dyes, both as a free and
immobilized, at high temperatures, without addition of harmful mediators, achieving maximum decolorization efciencies
of 99.0%, 71.2%, and 61.0% for Orange G (OG), Congo Red, and Eriochrome Black T (EBT), respectively. The immobilized
laccase on BSG was successfully reused across fve cycles of azo dye decolorization process. Notably, new green biocatalyst
outperformed commercial laccase from Aspergillus spp. in the decolorization of OG and EBT. GC-MS and LC-MS revealed
azo-dye degradation products and decomposition pathway. This analysis was complemented by antimicrobial and phytotoxicity tests, which confrmed the non-toxic nature of the degradation products, indicating the potential for safe environmental
disposal.",
publisher = "Springer",
journal = "Environmental Science and Pollution Research",
title = "Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain",
doi = "10.1007/s11356-024-33367-x"
}