TY  - JOUR
AU  - Žuža, Milena
AU  - Milašinović, Nikola
AU  - Jonović, Marko M.
AU  - Jovanović, Jelena
AU  - Kalagasidis Krušić, Melina
AU  - Bugarski, Branko
AU  - Knežević-Jugović, Zorica
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3544
AB  - In this study, alcalase (protease from Bacillus licheniformis) immobilization by adsorption, enzyme crosslinking and covalent enzyme binding to activated chitosan microbeads were examined. The biocatalysts highest activity was obtained by covalent immobilization of alcalase onto a solid support. The alcalase covalent immobilization onto different types of chitosan beads obtained by inverse emulsion technique and electrostatic extrusion was studied. Parameters examined under different conditions were beads diameter, enzyme loading, enzyme capacity yield, and biocatalyst activity. The highest activity and enzyme loading of 23.6 IU/mg protein and 340.2 mg/g, respectively, were achieved by the enzyme immobilized onto chitosan microbeads obtained by the electrostatic extrusion technique. FT-IR analysis was used to confirm formation of alcalase-chitosan conjugates. The activity of optimally produced alcalase-chitosan microbeads was then verified in the industrially feasible reaction systems of egg white and soy protein hydrolysis. The high degree of hydrolysis of 29.85 +/- 0.967% after 180 min and five successive reuses obtained under real conditions (50 A degrees C, pH 8) verified the covalently bound alcalase to chitosan beads a promising candidate for use in industrial egg white protein hydrolysis process.
PB  - Springer, New York
T2  - Bioprocess and Biosystems Engineering
T1  - Design and characterization of alcalase-chitosan conjugates as potential biocatalysts
EP  - 1723
IS  - 11
SP  - 1713
VL  - 40
DO  - 10.1007/s00449-017-1826-7
ER  - 

@article{
author = "Žuža, Milena and Milašinović, Nikola and Jonović, Marko M. and Jovanović, Jelena and Kalagasidis Krušić, Melina and Bugarski, Branko and Knežević-Jugović, Zorica",
year = "2017",
abstract = "In this study, alcalase (protease from Bacillus licheniformis) immobilization by adsorption, enzyme crosslinking and covalent enzyme binding to activated chitosan microbeads were examined. The biocatalysts highest activity was obtained by covalent immobilization of alcalase onto a solid support. The alcalase covalent immobilization onto different types of chitosan beads obtained by inverse emulsion technique and electrostatic extrusion was studied. Parameters examined under different conditions were beads diameter, enzyme loading, enzyme capacity yield, and biocatalyst activity. The highest activity and enzyme loading of 23.6 IU/mg protein and 340.2 mg/g, respectively, were achieved by the enzyme immobilized onto chitosan microbeads obtained by the electrostatic extrusion technique. FT-IR analysis was used to confirm formation of alcalase-chitosan conjugates. The activity of optimally produced alcalase-chitosan microbeads was then verified in the industrially feasible reaction systems of egg white and soy protein hydrolysis. The high degree of hydrolysis of 29.85 +/- 0.967% after 180 min and five successive reuses obtained under real conditions (50 A degrees C, pH 8) verified the covalently bound alcalase to chitosan beads a promising candidate for use in industrial egg white protein hydrolysis process.",
publisher = "Springer, New York",
journal = "Bioprocess and Biosystems Engineering",
title = "Design and characterization of alcalase-chitosan conjugates as potential biocatalysts",
pages = "1723-1713",
number = "11",
volume = "40",
doi = "10.1007/s00449-017-1826-7"
}

Žuža, M., Milašinović, N., Jonović, M. M., Jovanović, J., Kalagasidis Krušić, M., Bugarski, B.,& Knežević-Jugović, Z.. (2017). Design and characterization of alcalase-chitosan conjugates as potential biocatalysts. in Bioprocess and Biosystems Engineering
Springer, New York., 40(11), 1713-1723.
https://doi.org/10.1007/s00449-017-1826-7

Žuža M, Milašinović N, Jonović MM, Jovanović J, Kalagasidis Krušić M, Bugarski B, Knežević-Jugović Z. Design and characterization of alcalase-chitosan conjugates as potential biocatalysts. in Bioprocess and Biosystems Engineering. 2017;40(11):1713-1723.
doi:10.1007/s00449-017-1826-7 .

Žuža, Milena, Milašinović, Nikola, Jonović, Marko M., Jovanović, Jelena, Kalagasidis Krušić, Melina, Bugarski, Branko, Knežević-Jugović, Zorica, "Design and characterization of alcalase-chitosan conjugates as potential biocatalysts" in Bioprocess and Biosystems Engineering, 40, no. 11 (2017):1713-1723,
https://doi.org/10.1007/s00449-017-1826-7 . .