TY  - JOUR
AU  - Knežević, Aleksandar
AU  - Stajić, Mirjana
AU  - Sofrenić, Ivana V.
AU  - Stašić, Jelena M.
AU  - Milovanović, Ivan
AU  - Tešević, Vele
AU  - Vukojević, Jelena
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3849
AB  - In a last few decades mushrooms are increasingly attracting attention as functional food and sources of biologically active compounds. Several Trametes species have been used for centuries in traditional medicine of East Asia cultures, but only T. versicolorwas studied sufficiently while there are less substantial data about medicinal properties of other species. Trametes versicolor, T. hirsuta and T. gibbosa were the species tested for biological activities. Antifungal potentials of extracts were assessed for clinical strains of selected Candida and Aspergillus species. ABTS and FRAP assays were used to evaluate antioxidant capacities of studied extracts. Cytotoxic activity was determined against human cervix and lung adenocarcinoma and colon carcinoma cell lines. Antineurodegenerative activity was assessed by determining the rate of acetylcholinesterase and tyrosinase activity. The presence of metabolites in extracts of mycelia and basidiocarps of studied Trametes species was analyzed by H-1 NMR spectroscopy. Studied extracts showed low antifungal potential in comparison with ketoconazole. Basidiocarp extracts were more effective ABTS(+) scavengers and Fe2+ reducers than mycelium ones but less effective in comparison with L-ascorbic acid. Results showed that mycelium extracts had stronger cytotoxic effects against three cancer cell lines than basidiocarp ones, and that cervix adenocarcinoma cells were the most sensitive to the extracts and commercial cytostatics. T. versicolor mycelium extract was the most effective inhibitor of acetylcholinesterase activity but double weaker than galantamine, and T. gibbosa mycelium extract was significantly better inhibitor of tyrosinase activity than kojic acid for 40.9%. Chemical analysis indicated strong synergistic action of triterpenes, sugars and polyphenols in applied assays. The results suggest that tested Trametes species have significant medicinal potentials which could be attributed to antioxidative and cytotoxic activity. Additionally both, basidiocarps and mycelia extracts can strongly inhibit activity of acetylcholinesterase and tyrosinase.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia
IS  - 8
VL  - 13
DO  - 10.1371/journal.pone.0203064
ER  - 

@article{
author = "Knežević, Aleksandar and Stajić, Mirjana and Sofrenić, Ivana V. and Stašić, Jelena M. and Milovanović, Ivan and Tešević, Vele and Vukojević, Jelena",
year = "2018",
abstract = "In a last few decades mushrooms are increasingly attracting attention as functional food and sources of biologically active compounds. Several Trametes species have been used for centuries in traditional medicine of East Asia cultures, but only T. versicolorwas studied sufficiently while there are less substantial data about medicinal properties of other species. Trametes versicolor, T. hirsuta and T. gibbosa were the species tested for biological activities. Antifungal potentials of extracts were assessed for clinical strains of selected Candida and Aspergillus species. ABTS and FRAP assays were used to evaluate antioxidant capacities of studied extracts. Cytotoxic activity was determined against human cervix and lung adenocarcinoma and colon carcinoma cell lines. Antineurodegenerative activity was assessed by determining the rate of acetylcholinesterase and tyrosinase activity. The presence of metabolites in extracts of mycelia and basidiocarps of studied Trametes species was analyzed by H-1 NMR spectroscopy. Studied extracts showed low antifungal potential in comparison with ketoconazole. Basidiocarp extracts were more effective ABTS(+) scavengers and Fe2+ reducers than mycelium ones but less effective in comparison with L-ascorbic acid. Results showed that mycelium extracts had stronger cytotoxic effects against three cancer cell lines than basidiocarp ones, and that cervix adenocarcinoma cells were the most sensitive to the extracts and commercial cytostatics. T. versicolor mycelium extract was the most effective inhibitor of acetylcholinesterase activity but double weaker than galantamine, and T. gibbosa mycelium extract was significantly better inhibitor of tyrosinase activity than kojic acid for 40.9%. Chemical analysis indicated strong synergistic action of triterpenes, sugars and polyphenols in applied assays. The results suggest that tested Trametes species have significant medicinal potentials which could be attributed to antioxidative and cytotoxic activity. Additionally both, basidiocarps and mycelia extracts can strongly inhibit activity of acetylcholinesterase and tyrosinase.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia",
number = "8",
volume = "13",
doi = "10.1371/journal.pone.0203064"
}

Knežević, A., Stajić, M., Sofrenić, I. V., Stašić, J. M., Milovanović, I., Tešević, V.,& Vukojević, J.. (2018). Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia. in PLoS One
Public Library Science, San Francisco., 13(8).
https://doi.org/10.1371/journal.pone.0203064

Knežević A, Stajić M, Sofrenić IV, Stašić JM, Milovanović I, Tešević V, Vukojević J. Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia. in PLoS One. 2018;13(8).
doi:10.1371/journal.pone.0203064 .

Knežević, Aleksandar, Stajić, Mirjana, Sofrenić, Ivana V., Stašić, Jelena M., Milovanović, Ivan, Tešević, Vele, Vukojević, Jelena, "Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia" in PLoS One, 13, no. 8 (2018),
https://doi.org/10.1371/journal.pone.0203064 . .

TY  - JOUR
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Vujisić, Ljubodrag V.
AU  - Veličković, Dušan
AU  - Bezbradica, Dejan
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3593
AB  - Solubility and stability of flavonoid glycosides, valuable natural constituents of cosmetics and pharmaceuticals, could be improved by lipase-catalyzed acylation. Focus of this study was on development of eco-friendly process for the production of flavonoid acetates. By using phloridzin as model compound and triacetin as acetyl donor and solvent, 100% conversion and high productivity (23.32 gl(-1) day(-1)) were accomplished. Complete conversions of two other glycosylated flavonoids, naringin and esculin, in solvent-free system were achieved, as well. Comprehensive kinetic mechanism based on two con-secutive mono-substrate reactions was established where first one represents formation of flavonoid monoacetate and within second reaction diacetate is being produced from monoacetate. Both steps were regarded as reversible Michaelis-Menten reactions without inhibition. Apparent kinetic parameters for two consecutive reactions (V-m constants for substrates and products and Km constants for forward and reverse reactions) were estimated for three examined acetyl acceptors and excellent fitting of experimental data (R-2 gt 0.97) was achieved. Obtained results showed that derived kinetic model could be applicable for solvent-free esterifications of different flavonoid glycosides. It was valid for entire transesterification course (72 h of reaction) which, combined with complete conversions and green character of synthesis, represents firm basis for further process development.
PB  - Elsevier Science Bv, Amsterdam
T2  - Biochemical Engineering Journal
T1  - Highly efficient enzymatic acetylation of flavonoids: Development of solvent-free process and kinetic evaluation
EP  - 115
SP  - 106
VL  - 128
DO  - 10.1016/j.bej.2017.09.018
ER  - 

@article{
author = "Milivojević, Ana and Ćorović, Marija and Simović, Milica and Banjanac, Katarina and Vujisić, Ljubodrag V. and Veličković, Dušan and Bezbradica, Dejan",
year = "2017",
abstract = "Solubility and stability of flavonoid glycosides, valuable natural constituents of cosmetics and pharmaceuticals, could be improved by lipase-catalyzed acylation. Focus of this study was on development of eco-friendly process for the production of flavonoid acetates. By using phloridzin as model compound and triacetin as acetyl donor and solvent, 100% conversion and high productivity (23.32 gl(-1) day(-1)) were accomplished. Complete conversions of two other glycosylated flavonoids, naringin and esculin, in solvent-free system were achieved, as well. Comprehensive kinetic mechanism based on two con-secutive mono-substrate reactions was established where first one represents formation of flavonoid monoacetate and within second reaction diacetate is being produced from monoacetate. Both steps were regarded as reversible Michaelis-Menten reactions without inhibition. Apparent kinetic parameters for two consecutive reactions (V-m constants for substrates and products and Km constants for forward and reverse reactions) were estimated for three examined acetyl acceptors and excellent fitting of experimental data (R-2 gt 0.97) was achieved. Obtained results showed that derived kinetic model could be applicable for solvent-free esterifications of different flavonoid glycosides. It was valid for entire transesterification course (72 h of reaction) which, combined with complete conversions and green character of synthesis, represents firm basis for further process development.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Biochemical Engineering Journal",
title = "Highly efficient enzymatic acetylation of flavonoids: Development of solvent-free process and kinetic evaluation",
pages = "115-106",
volume = "128",
doi = "10.1016/j.bej.2017.09.018"
}

Milivojević, A., Ćorović, M., Simović, M., Banjanac, K., Vujisić, L. V., Veličković, D.,& Bezbradica, D.. (2017). Highly efficient enzymatic acetylation of flavonoids: Development of solvent-free process and kinetic evaluation. in Biochemical Engineering Journal
Elsevier Science Bv, Amsterdam., 128, 106-115.
https://doi.org/10.1016/j.bej.2017.09.018

Milivojević A, Ćorović M, Simović M, Banjanac K, Vujisić LV, Veličković D, Bezbradica D. Highly efficient enzymatic acetylation of flavonoids: Development of solvent-free process and kinetic evaluation. in Biochemical Engineering Journal. 2017;128:106-115.
doi:10.1016/j.bej.2017.09.018 .

Milivojević, Ana, Ćorović, Marija, Simović, Milica, Banjanac, Katarina, Vujisić, Ljubodrag V., Veličković, Dušan, Bezbradica, Dejan, "Highly efficient enzymatic acetylation of flavonoids: Development of solvent-free process and kinetic evaluation" in Biochemical Engineering Journal, 128 (2017):106-115,
https://doi.org/10.1016/j.bej.2017.09.018 . .

TY  - JOUR
AU  - Aljančić, Ivana
AU  - Pešić, Milica
AU  - Milosavljević, Slobodan M.
AU  - Todorović, Nina
AU  - Jadranin, Milka
AU  - Miosavljević, Goran
AU  - Povrenović, Dragan
AU  - Banković, Jasna
AU  - Tanić, Nikola
AU  - Marković, Ivanka
AU  - Ruzdijić, Sabera
AU  - Vajs, Vlatka
AU  - Tešević, Vele
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1943
AB  - From the Montenegrin spurge Euphorbia dendroides, seven new diterpenoids [jatrophanes (1-6) and a tigliane (7)] were isolated and their structures elucidated by spectroscopic techniques. The biological activity of the new compounds was studied against four human cancer cell lines. The most effective jatrophane-type compound (2) and its structurally closely related derivative (1) were evaluated for their interactions with paclitaxel and doxorubicin using a multidrug-resistant cancer cell line. Both compounds exerted a strong reversal potential resulting from inhibition of P-glycoprotein transport.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Natural Products
T1  - Isolation and Biological Evaluation of Jatrophane Diterpenoids from Euphorbia dendroides
EP  - 1620
IS  - 7
SP  - 1613
VL  - 74
DO  - 10.1021/np200241c
ER  - 

@article{
author = "Aljančić, Ivana and Pešić, Milica and Milosavljević, Slobodan M. and Todorović, Nina and Jadranin, Milka and Miosavljević, Goran and Povrenović, Dragan and Banković, Jasna and Tanić, Nikola and Marković, Ivanka and Ruzdijić, Sabera and Vajs, Vlatka and Tešević, Vele",
year = "2011",
abstract = "From the Montenegrin spurge Euphorbia dendroides, seven new diterpenoids [jatrophanes (1-6) and a tigliane (7)] were isolated and their structures elucidated by spectroscopic techniques. The biological activity of the new compounds was studied against four human cancer cell lines. The most effective jatrophane-type compound (2) and its structurally closely related derivative (1) were evaluated for their interactions with paclitaxel and doxorubicin using a multidrug-resistant cancer cell line. Both compounds exerted a strong reversal potential resulting from inhibition of P-glycoprotein transport.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Natural Products",
title = "Isolation and Biological Evaluation of Jatrophane Diterpenoids from Euphorbia dendroides",
pages = "1620-1613",
number = "7",
volume = "74",
doi = "10.1021/np200241c"
}

Aljančić, I., Pešić, M., Milosavljević, S. M., Todorović, N., Jadranin, M., Miosavljević, G., Povrenović, D., Banković, J., Tanić, N., Marković, I., Ruzdijić, S., Vajs, V.,& Tešević, V.. (2011). Isolation and Biological Evaluation of Jatrophane Diterpenoids from Euphorbia dendroides. in Journal of Natural Products
Amer Chemical Soc, Washington., 74(7), 1613-1620.
https://doi.org/10.1021/np200241c

Aljančić I, Pešić M, Milosavljević SM, Todorović N, Jadranin M, Miosavljević G, Povrenović D, Banković J, Tanić N, Marković I, Ruzdijić S, Vajs V, Tešević V. Isolation and Biological Evaluation of Jatrophane Diterpenoids from Euphorbia dendroides. in Journal of Natural Products. 2011;74(7):1613-1620.
doi:10.1021/np200241c .

Aljančić, Ivana, Pešić, Milica, Milosavljević, Slobodan M., Todorović, Nina, Jadranin, Milka, Miosavljević, Goran, Povrenović, Dragan, Banković, Jasna, Tanić, Nikola, Marković, Ivanka, Ruzdijić, Sabera, Vajs, Vlatka, Tešević, Vele, "Isolation and Biological Evaluation of Jatrophane Diterpenoids from Euphorbia dendroides" in Journal of Natural Products, 74, no. 7 (2011):1613-1620,
https://doi.org/10.1021/np200241c . .