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Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery

Kostić, Ivana T.; Ilić, Vesna Lj.; Bukara, Katarina; Mojsilović, Slavko B.; Đurić, Zorka Ž.; Draškovič, Petra; Bugarski, Branko

(Association of Chemical Engineers of Serbia, 2015)

TY  - JOUR
AU  - Kostić, Ivana T.
AU  - Ilić, Vesna Lj.
AU  - Bukara, Katarina
AU  - Mojsilović, Slavko B.
AU  - Đurić, Zorka Ž.
AU  - Draškovič, Petra
AU  - Bugarski, Branko
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2900
AB  - Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bovine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected.
AB  - Uprkos činjenici da su metode koje se baziraju na osmotskim osobinama ćelija najčešće korišćene metode za inkapsulaciju lekova u humane i životinjske eritrocite, podaci o osmotskim osobinama eritrocita životinjskog porekla su vrlo oskudni. Cilj ovog rada bio je ispitivanje mogućnosti korišćenja metode protočne citometrije za određivanje osmotskih osobina svinjskih i goveđih eritrocita, čime bi se olakšao inženjering pomenutih životinjskih eritrocita za otpuštanje lekova. Metodom protočne citometrije uspešno su dobijene informacije o osmotskoj fragilnosti svinjskih i goveđih eritrocita i načinjeni su početni koraci u proceni oblika velikog broja eritrocita. Iako ova metoda nije uspela da potvrdi bubrenje svinjskih eritrocita, ukazala je na razliku u uzorcima svinjskih eritrocita koji su imali osnovne hematološke parametre izvan i unutar referentnih vrednosti. U cilju primene svinjskih i goveđih eritrocita kao nosača lekova, metoda protočne citometrije je, potvrdivši prisustvo osmotski različitih frakcija eritrocita, ukazala na to da se različite količine inkapsuliranog leka u pojedinačnim, kako svinjskim, tako i goveđim eritrocitima mogu očekivati.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery
T1  - Određivanje osmotskih osobina životinjskih eritrocita protočnom citometrijom u cilju njihovog inženjeringa kao nosača lekova
EP  - 76
IS  - 1
SP  - 67
VL  - 69
DO  - 10.2298/HEMIND140124021K
ER  - 
@article{
author = "Kostić, Ivana T. and Ilić, Vesna Lj. and Bukara, Katarina and Mojsilović, Slavko B. and Đurić, Zorka Ž. and Draškovič, Petra and Bugarski, Branko",
year = "2015",
abstract = "Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bovine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected., Uprkos činjenici da su metode koje se baziraju na osmotskim osobinama ćelija najčešće korišćene metode za inkapsulaciju lekova u humane i životinjske eritrocite, podaci o osmotskim osobinama eritrocita životinjskog porekla su vrlo oskudni. Cilj ovog rada bio je ispitivanje mogućnosti korišćenja metode protočne citometrije za određivanje osmotskih osobina svinjskih i goveđih eritrocita, čime bi se olakšao inženjering pomenutih životinjskih eritrocita za otpuštanje lekova. Metodom protočne citometrije uspešno su dobijene informacije o osmotskoj fragilnosti svinjskih i goveđih eritrocita i načinjeni su početni koraci u proceni oblika velikog broja eritrocita. Iako ova metoda nije uspela da potvrdi bubrenje svinjskih eritrocita, ukazala je na razliku u uzorcima svinjskih eritrocita koji su imali osnovne hematološke parametre izvan i unutar referentnih vrednosti. U cilju primene svinjskih i goveđih eritrocita kao nosača lekova, metoda protočne citometrije je, potvrdivši prisustvo osmotski različitih frakcija eritrocita, ukazala na to da se različite količine inkapsuliranog leka u pojedinačnim, kako svinjskim, tako i goveđim eritrocitima mogu očekivati.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery, Određivanje osmotskih osobina životinjskih eritrocita protočnom citometrijom u cilju njihovog inženjeringa kao nosača lekova",
pages = "76-67",
number = "1",
volume = "69",
doi = "10.2298/HEMIND140124021K"
}
Kostić, I. T., Ilić, V. Lj., Bukara, K., Mojsilović, S. B., Đurić, Z. Ž., Draškovič, P.,& Bugarski, B.. (2015). Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery. in Hemijska industrija
Association of Chemical Engineers of Serbia., 69(1), 67-76.
https://doi.org/10.2298/HEMIND140124021K
Kostić IT, Ilić VL, Bukara K, Mojsilović SB, Đurić ZŽ, Draškovič P, Bugarski B. Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery. in Hemijska industrija. 2015;69(1):67-76.
doi:10.2298/HEMIND140124021K .
Kostić, Ivana T., Ilić, Vesna Lj., Bukara, Katarina, Mojsilović, Slavko B., Đurić, Zorka Ž., Draškovič, Petra, Bugarski, Branko, "Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery" in Hemijska industrija, 69, no. 1 (2015):67-76,
https://doi.org/10.2298/HEMIND140124021K . .
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