TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Obradović, Hristina
AU  - Bugarski, Branko
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Diana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4483
AB  - We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.
PB  - Elsevier, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro
EP  - 920
SP  - 909
VL  - 144
DO  - 10.1016/j.ijbiomac.2019.09.167
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Obradović, Hristina and Bugarski, Branko and Ilić, Vesna Lj. and Bugarski, Diana",
year = "2020",
abstract = "We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro",
pages = "920-909",
volume = "144",
doi = "10.1016/j.ijbiomac.2019.09.167"
}

Stančić, A., Drvenica, I., Obradović, H., Bugarski, B., Ilić, V. Lj.,& Bugarski, D.. (2020). Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules
Elsevier, Amsterdam., 144, 909-920.
https://doi.org/10.1016/j.ijbiomac.2019.09.167

Stančić A, Drvenica I, Obradović H, Bugarski B, Ilić VL, Bugarski D. Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules. 2020;144:909-920.
doi:10.1016/j.ijbiomac.2019.09.167 .

Stančić, Ana, Drvenica, Ivana, Obradović, Hristina, Bugarski, Branko, Ilić, Vesna Lj., Bugarski, Diana, "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro" in International Journal of Biological Macromolecules, 144 (2020):909-920,
https://doi.org/10.1016/j.ijbiomac.2019.09.167 . .

TY  - CONF
AU  - Marković, Maja
AU  - Panić, Vesna
AU  - Šešlija, Sanja
AU  - Spasojević, Pavle
AU  - Ugrinović, Vukašin
AU  - Bošković-Vragolović, Nevenka
AU  - Pjanović, Rada
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5075
AB  - Soft polymeric networks based on poly(methacrylic
acid) (PMAA) are attractive candidates for targeted and
controlled drug release due to their non-toxicity,
biocompatibility and pH-sensitivity. The highly hydrophilic
nature of PMAA networks enables transport of hydrophilic
drugs only. This limitation has been overcome in present work
by a PMAA modification with casein and liposomes. Casein is a
natural amphiphilic protein which enabled the encapsulation
and targeted and controlled release of the model drug- caffeine.
The FTIR spectra showed that the hydrophobic interactions and
hydrogen bonds were established between the casein and
caffeine. The caffeine in vitro release was monitored in two media
at 37C: phosphate buffer pH=6.8, which simulated the pH
environment in the human intestines and 0.1M HCl pH=1.2,
which simulated the pH environment in the human stomach. The
presence of liposomes with the encapsulated caffeine in the
carriers caused the decrease in the speed of caffeine release.
Introduction of itaconic acid (IA) as a hydrophilic and pHsensitive substance with two carboxylic groups resulted in a nonregular structure of the carriers with large voids which caused
an increase in swelling rate of the carriers and an increase in
speed of caffeine release. All obtained results showed that the
targeted and controlled release of a poorly water-soluble
substance was achieved.
PB  - ETRAN Society, Belgrade, Academic Mind, Belgrade
C3  - 6th International Conference on Electrical, Electronic and Computing Engineering, IcETRAN 2019
T1  - Soft polymeric networks based on poly(methacrylic acid), itaconic acid, casein and liposomes for targeted delivery and controlled release of poorly water soluble active substance
EP  - 670
SP  - 665
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5075
ER  - 

@conference{
author = "Marković, Maja and Panić, Vesna and Šešlija, Sanja and Spasojević, Pavle and Ugrinović, Vukašin and Bošković-Vragolović, Nevenka and Pjanović, Rada",
year = "2019",
abstract = "Soft polymeric networks based on poly(methacrylic
acid) (PMAA) are attractive candidates for targeted and
controlled drug release due to their non-toxicity,
biocompatibility and pH-sensitivity. The highly hydrophilic
nature of PMAA networks enables transport of hydrophilic
drugs only. This limitation has been overcome in present work
by a PMAA modification with casein and liposomes. Casein is a
natural amphiphilic protein which enabled the encapsulation
and targeted and controlled release of the model drug- caffeine.
The FTIR spectra showed that the hydrophobic interactions and
hydrogen bonds were established between the casein and
caffeine. The caffeine in vitro release was monitored in two media
at 37C: phosphate buffer pH=6.8, which simulated the pH
environment in the human intestines and 0.1M HCl pH=1.2,
which simulated the pH environment in the human stomach. The
presence of liposomes with the encapsulated caffeine in the
carriers caused the decrease in the speed of caffeine release.
Introduction of itaconic acid (IA) as a hydrophilic and pHsensitive substance with two carboxylic groups resulted in a nonregular structure of the carriers with large voids which caused
an increase in swelling rate of the carriers and an increase in
speed of caffeine release. All obtained results showed that the
targeted and controlled release of a poorly water-soluble
substance was achieved.",
publisher = "ETRAN Society, Belgrade, Academic Mind, Belgrade",
journal = "6th International Conference on Electrical, Electronic and Computing Engineering, IcETRAN 2019",
title = "Soft polymeric networks based on poly(methacrylic acid), itaconic acid, casein and liposomes for targeted delivery and controlled release of poorly water soluble active substance",
pages = "670-665",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5075"
}

Marković, M., Panić, V., Šešlija, S., Spasojević, P., Ugrinović, V., Bošković-Vragolović, N.,& Pjanović, R.. (2019). Soft polymeric networks based on poly(methacrylic acid), itaconic acid, casein and liposomes for targeted delivery and controlled release of poorly water soluble active substance. in 6th International Conference on Electrical, Electronic and Computing Engineering, IcETRAN 2019
ETRAN Society, Belgrade, Academic Mind, Belgrade., 665-670.
https://hdl.handle.net/21.15107/rcub_technorep_5075

Marković M, Panić V, Šešlija S, Spasojević P, Ugrinović V, Bošković-Vragolović N, Pjanović R. Soft polymeric networks based on poly(methacrylic acid), itaconic acid, casein and liposomes for targeted delivery and controlled release of poorly water soluble active substance. in 6th International Conference on Electrical, Electronic and Computing Engineering, IcETRAN 2019. 2019;:665-670.
https://hdl.handle.net/21.15107/rcub_technorep_5075 .

Marković, Maja, Panić, Vesna, Šešlija, Sanja, Spasojević, Pavle, Ugrinović, Vukašin, Bošković-Vragolović, Nevenka, Pjanović, Rada, "Soft polymeric networks based on poly(methacrylic acid), itaconic acid, casein and liposomes for targeted delivery and controlled release of poorly water soluble active substance" in 6th International Conference on Electrical, Electronic and Computing Engineering, IcETRAN 2019 (2019):665-670,
https://hdl.handle.net/21.15107/rcub_technorep_5075 .