@conference{
author = "Lukić, Ivana and Pajnik, Jelena and Milovanović, Stoja and Tadić, Vanja",
year = "2022",
abstract = "Bilberries (Vaccinium myrtillus) are believed to have one of the highest antioxidant levels of all
common fruits and vegetables. As a source of antioxidants, they have been used in traditional
medicine to reduce inflammation and protect against diseases associated with oxidative stress, such
as cardiovascular disease, diabetes, and age-related cognitive decline, as well as a remedy for eye
conditions. The biological activity of bilberries is associated with the high content of anthocyanins,
a large group of water-soluble flavonoids. Besides the complex anthocyanin profile, bilberries also
contain other phenolic compounds including flavonols, tannins, and phenolic acids. Supercritical fluid
extraction (SFE) isa a green and environmentally friendly process based on the use of supercritical
fluids, most commonly CO2, as an alternative to organic solvents, to obtain solvent-free and highly
valuable plant extracts. Utilization of supercritical CO2 (scCO2) extraction also enables the preservation
of thermally labile compounds working at low temperatures, and good selectivity accomplished by
the appropriate choice of operating pressure and temperature. In this study, scCO2 extraction was
investigated as a method for isolating the extract from bilberry dried fruit. Extractions were performed
at temperature of 70 °C and pressures of 15 and 30 MPa, with and without the ethanol as co-solvent,
with the aim to optimize the yield of the obtained extract based on the composition. The obtained
extraction yields ranged from 1.2 to 7% and the highest yield was obtained, as expected, at a higher
pressure of 30 MPa. The effect of ethanol as co-solvent in the scCO2 extraction was studied with the
goal to modify scCO2 selectivity and enhance the solubility of polar substances. The results showed a
positive effect on the extraction yield, while total phenolic content (TPC) analysis revealed that extracts
obtained using co-solvent have higher TPC values. Chemical analysis of the obtained extracts was
performed in order to qualitatively evaluate their composition.",
publisher = "Zagreb : University, Faculty of Food Technology and Biotechnology",
journal = "Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022",
title = "SUPERCRITICAL CO2 EXTRACTION OF BILBERRY (Vaccinium myrtillus) FRUIT",
pages = "100",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6905"
}