Alliance of International Science Organizations, project SparkGREEN (ANSO-CR-PP 2022-08)

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Alliance of International Science Organizations, project SparkGREEN (ANSO-CR-PP 2022-08)

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Delignifikacija kukuruzne stabljike primenom zelenih rastvarača

Grbić, Jovana; Mladenović, Dragana; Lazović, Saša; Đukić-Vuković, Aleksandra

(Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi = National Society of Processing and Energy in Agriculture, 2024)

TY  - CONF
AU  - Grbić, Jovana
AU  - Mladenović, Dragana
AU  - Lazović, Saša
AU  - Đukić-Vuković, Aleksandra
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7445
AB  - Lignoceluloza čini glavnu komponentu poljoprivrednog i organskog komunalnog otpada. Zahvaljujući svom sastavu, koji uključuje visok sadržaj šećera, dostupnosti i biorazgradivosti, predstavlja kvalitetnu polaznu sirovinu u biorafinerijskim procesima. Razgradnja kompleksne lignocelulozne strukture je ključni korak u njenoj valorizaciji. Konvencionalne metode za razgradnju lignoceluloze zasnivaju se na upotrebi jakih kiselina i baza i/ili visokih temperatura i pritisaka, čineći ih energetski, ekonomski i ekološki nepovoljnim. Jedan od načina da se ovaj problem prevaziđe je upotreba tzv. „zelenih“ rastvarača, poput vode i etanola. Predmet ovog rada je upotreba zelenih rastvarača u kombinaciji sa gasnom plazmom za razgradnju lignocelulozne biomase pri atmosferskim uslovima. Kukuruzna stabljika je korišćena kao model supstrat, pripremljen sa vodom ili 96% etanolom kao rastvaračima. Za tretman gasnom plazmom korišćena je netermalna gasna plazma igla. Efikasnost primenjenih tretmana praćena je sa aspekta delignifikacije kukuruzne stabljike i naknadne enzimske hidrolize tretirane frakcije. Sadržaj lignina u uzorcima nakon tretmana određivan je na osnovu njegove rastvorljivosti u acetil bromidu (ABSL – acetyl-bromide soluble lignin, engl.). Frakcije dobijene nakon tretmana su podvrgnute enzimskoj hidrolizi sa komercijalnom smešom celulaza, hemicelulaza i ?-galaktozidaza, Cellic CTec 2 (Novozymes, Danska). Nakon 48-časovne hidrolize u dobijenim hidrolizatima je određivan sadržaj heksoza i pentoza. Sadržaj lignina smanjen je za 40% tokom tretmana plazmom u trajanju od 30 minuta u etanolu. U slučaju vode razgrađeno je 20% lignina pri istoj dužini tretmana. Međutim, produžavanjem dužine tretmana na 60 minuta u vodi postignuta je značajna delignifikacija, smanjenjem sadržaja lignina za 50% u odnosu na netretiranu biomasu. Dodatno produžavanje tretmana plazmom na 90 minuta nije dovelo do povećanja stepena delignifikacije. Prinosi šećera ostvareni nakon hidrolize su u skladu sa delignifikacijom, pri čemu je najveći prinos postignut nakon plazma tretmana u etanolu tokom 30 minuta, odnosno u vodi tokom 60 minuta. Primenom kombinovanog plazma tretmana moguće je razgraditi lignin i olakšati prodor enzima do ugljeno-hidratne komponente lignoceluloze pri atmosferskim uslovima, bez upotrebe jakih kiselina, baza ili organskih rastvarača. Upotrebom navedenih zelenih rastvarača smanjuje se potreba za dodatnim koracima prečišćavanja tretirane biomase, a i naknadne faze enzimske hidrolize i fermentacije su značajno jednostavnije. Osim toga, moguća je rekuperacija iskorišćenog etanola, što je u skladu sa principima cirkularne bioekonomije.
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi = National Society of Processing and Energy in Agriculture
C3  - Knjiga sažetaka = Book of Abstracts / XXXVI Naučno-stručni skup Procesna tehnika i energetika u poljoprivredi - PTEP 2024 = XXXVI Scientific - Professional Conference Processing and Energy in Agriculture - PTEP 2024, Tara, Hotel Omorika, 15 – 18. April 2024
T1  - Delignifikacija kukuruzne stabljike primenom zelenih rastvarača
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7445
ER  - 
@conference{
author = "Grbić, Jovana and Mladenović, Dragana and Lazović, Saša and Đukić-Vuković, Aleksandra",
year = "2024",
abstract = "Lignoceluloza čini glavnu komponentu poljoprivrednog i organskog komunalnog otpada. Zahvaljujući svom sastavu, koji uključuje visok sadržaj šećera, dostupnosti i biorazgradivosti, predstavlja kvalitetnu polaznu sirovinu u biorafinerijskim procesima. Razgradnja kompleksne lignocelulozne strukture je ključni korak u njenoj valorizaciji. Konvencionalne metode za razgradnju lignoceluloze zasnivaju se na upotrebi jakih kiselina i baza i/ili visokih temperatura i pritisaka, čineći ih energetski, ekonomski i ekološki nepovoljnim. Jedan od načina da se ovaj problem prevaziđe je upotreba tzv. „zelenih“ rastvarača, poput vode i etanola. Predmet ovog rada je upotreba zelenih rastvarača u kombinaciji sa gasnom plazmom za razgradnju lignocelulozne biomase pri atmosferskim uslovima. Kukuruzna stabljika je korišćena kao model supstrat, pripremljen sa vodom ili 96% etanolom kao rastvaračima. Za tretman gasnom plazmom korišćena je netermalna gasna plazma igla. Efikasnost primenjenih tretmana praćena je sa aspekta delignifikacije kukuruzne stabljike i naknadne enzimske hidrolize tretirane frakcije. Sadržaj lignina u uzorcima nakon tretmana određivan je na osnovu njegove rastvorljivosti u acetil bromidu (ABSL – acetyl-bromide soluble lignin, engl.). Frakcije dobijene nakon tretmana su podvrgnute enzimskoj hidrolizi sa komercijalnom smešom celulaza, hemicelulaza i ?-galaktozidaza, Cellic CTec 2 (Novozymes, Danska). Nakon 48-časovne hidrolize u dobijenim hidrolizatima je određivan sadržaj heksoza i pentoza. Sadržaj lignina smanjen je za 40% tokom tretmana plazmom u trajanju od 30 minuta u etanolu. U slučaju vode razgrađeno je 20% lignina pri istoj dužini tretmana. Međutim, produžavanjem dužine tretmana na 60 minuta u vodi postignuta je značajna delignifikacija, smanjenjem sadržaja lignina za 50% u odnosu na netretiranu biomasu. Dodatno produžavanje tretmana plazmom na 90 minuta nije dovelo do povećanja stepena delignifikacije. Prinosi šećera ostvareni nakon hidrolize su u skladu sa delignifikacijom, pri čemu je najveći prinos postignut nakon plazma tretmana u etanolu tokom 30 minuta, odnosno u vodi tokom 60 minuta. Primenom kombinovanog plazma tretmana moguće je razgraditi lignin i olakšati prodor enzima do ugljeno-hidratne komponente lignoceluloze pri atmosferskim uslovima, bez upotrebe jakih kiselina, baza ili organskih rastvarača. Upotrebom navedenih zelenih rastvarača smanjuje se potreba za dodatnim koracima prečišćavanja tretirane biomase, a i naknadne faze enzimske hidrolize i fermentacije su značajno jednostavnije. Osim toga, moguća je rekuperacija iskorišćenog etanola, što je u skladu sa principima cirkularne bioekonomije.",
publisher = "Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi = National Society of Processing and Energy in Agriculture",
journal = "Knjiga sažetaka = Book of Abstracts / XXXVI Naučno-stručni skup Procesna tehnika i energetika u poljoprivredi - PTEP 2024 = XXXVI Scientific - Professional Conference Processing and Energy in Agriculture - PTEP 2024, Tara, Hotel Omorika, 15 – 18. April 2024",
title = "Delignifikacija kukuruzne stabljike primenom zelenih rastvarača",
pages = "18",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7445"
}
Grbić, J., Mladenović, D., Lazović, S.,& Đukić-Vuković, A.. (2024). Delignifikacija kukuruzne stabljike primenom zelenih rastvarača. in Knjiga sažetaka = Book of Abstracts / XXXVI Naučno-stručni skup Procesna tehnika i energetika u poljoprivredi - PTEP 2024 = XXXVI Scientific - Professional Conference Processing and Energy in Agriculture - PTEP 2024, Tara, Hotel Omorika, 15 – 18. April 2024
Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi = National Society of Processing and Energy in Agriculture., 18.
https://hdl.handle.net/21.15107/rcub_technorep_7445
Grbić J, Mladenović D, Lazović S, Đukić-Vuković A. Delignifikacija kukuruzne stabljike primenom zelenih rastvarača. in Knjiga sažetaka = Book of Abstracts / XXXVI Naučno-stručni skup Procesna tehnika i energetika u poljoprivredi - PTEP 2024 = XXXVI Scientific - Professional Conference Processing and Energy in Agriculture - PTEP 2024, Tara, Hotel Omorika, 15 – 18. April 2024. 2024;:18.
https://hdl.handle.net/21.15107/rcub_technorep_7445 .
Grbić, Jovana, Mladenović, Dragana, Lazović, Saša, Đukić-Vuković, Aleksandra, "Delignifikacija kukuruzne stabljike primenom zelenih rastvarača" in Knjiga sažetaka = Book of Abstracts / XXXVI Naučno-stručni skup Procesna tehnika i energetika u poljoprivredi - PTEP 2024 = XXXVI Scientific - Professional Conference Processing and Energy in Agriculture - PTEP 2024, Tara, Hotel Omorika, 15 – 18. April 2024 (2024):18,
https://hdl.handle.net/21.15107/rcub_technorep_7445 .

PLAZMA AKTIVIRANI VODENI EKSTRAKATI CVETA KOPRIVE KAO SUPSTRAT ZA GAJENJE BIOMASE LIGILACTOBACILLUS SALIVARIUS

Bogdanović, Mihajlo; Mladenović, Dragana; Grbić, Jovana; Mladenović, Mihailo; Lazović, Saša; Đukić-Vuković, Aleksandra

(Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi = National Society of Processing and Energy in Agriculture, 2024)

TY  - CONF
AU  - Bogdanović, Mihajlo
AU  - Mladenović, Dragana
AU  - Grbić, Jovana
AU  - Mladenović, Mihailo
AU  - Lazović, Saša
AU  - Đukić-Vuković, Aleksandra
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7446
AB  - Kopriva (Urtica dioica) je samonikla biljka bogata flavonoidima, karotenoidima, fitosterolima,
terpenima. Ima izuzetan farmaceutski potencijal zbog svojih antiinflamatornih, antioksidativnih i
diuretskih svojstava. Koristi se u ishrani, kao caj ili u formi suvih ili tecnih ekstrakta. U cilju
pobiljšanja karakteristika, bioraspoloživosti i stabilnosti ekstrakata, koriste se ekstrakcije ekološki
prihvatljivim rastvaracima, zeleni fizicki tretmani i fermentacija probiotickim bakterijama. Zeleni
rastvaraci kao što su voda, etanol i biokompatibilni eutekticke tecnosti su pogodni za dobijanje
ekstrakata u skladu sa principima održivog razvoja. Inovativni fizicki tretmani kao netermalni
tretman plazmom mogu obezbediti bolju ekstrakciju i mikrobiološku stabilnost posebno vodenih
ekstrakata. Takode, fermentacije bakterijama, kao što je Ligilactobacillus salivarius, mogu da
metabolišu jedinjenja prisutna u ekstraktima i biotransformišu glikozide, tanine i lignane u biološki
aktivnije oblike.
U radu smo ispitivali rast i antioksidativnu aktivnost vodenih ekstrakata cveta koprive dobijenih uz
tretman netermalnom plazmom i potom fermentaciju sa L. salivarius. Ispitali smo rast L. salivarius
u medijumima sa razlicitim udelom vodenog ekstrata cveta koprive i Man Rogosa Šarp (MRS) bujona
koji je koristi kao standardni mikrobiološki medijum za kultivaciju bakterija mlecne kiseline.
Antioksidativna aktivnost dobijenih ekstrakata je ispitana DPPH metodom, pre i nakon fermentacije.
L. salivarius je pokazao najbolji rast (11 log CFU/ml) u ekstraktima obogacenim sa 25% MRS. Potom
je je ispitivan rast L. salivarius u plazma tretiranom vodenom ekstraktu koprive sa 25% MRS. Nakon
tretmana netermalnom plazmom od 3 min i naknadne fermentacije ovih ekstrakata, postignut je rast
od 9 log CFU/ml i porast antioksidativne aktivnosti od 27% DPPH metodom. Uocen je pozitivan
kombinovani efekat netermalne plazme i fermentacije na povecanje antioksidativne aktivnosti,
posebno pri kracim tretmanima netermalnom plazmom. Rezultati rada ukazuju na mogucnost
primene zelenih tretmana i fermentacije za dobijanje proizvoda ekstrakta cveta koprive obogacenih
sa L. salivarius. Plazma tretman može uticati i na mikrobiološki sastav vodenih ekstrakata, kao
važnog aspekta za primenu u prehrambenoj i farmaceutskoj industriji.
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi = National Society of Processing and Energy in Agriculture
C3  - Knjiga sažetaka = Book of Abstracts / XXXVI Naučno-stručni skup Procesna tehnika i energetika u poljoprivredi - PTEP 2024 = XXXVI Scientific - Professional Conference Processing and Energy in Agriculture - PTEP 2024, Tara, Hotel Omorika, 15 – 18. April 2024
T1  - PLAZMA AKTIVIRANI VODENI EKSTRAKATI CVETA KOPRIVE KAO SUPSTRAT ZA GAJENJE BIOMASE LIGILACTOBACILLUS SALIVARIUS
SP  - 6
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7446
ER  - 
@conference{
author = "Bogdanović, Mihajlo and Mladenović, Dragana and Grbić, Jovana and Mladenović, Mihailo and Lazović, Saša and Đukić-Vuković, Aleksandra",
year = "2024",
abstract = "Kopriva (Urtica dioica) je samonikla biljka bogata flavonoidima, karotenoidima, fitosterolima,
terpenima. Ima izuzetan farmaceutski potencijal zbog svojih antiinflamatornih, antioksidativnih i
diuretskih svojstava. Koristi se u ishrani, kao caj ili u formi suvih ili tecnih ekstrakta. U cilju
pobiljšanja karakteristika, bioraspoloživosti i stabilnosti ekstrakata, koriste se ekstrakcije ekološki
prihvatljivim rastvaracima, zeleni fizicki tretmani i fermentacija probiotickim bakterijama. Zeleni
rastvaraci kao što su voda, etanol i biokompatibilni eutekticke tecnosti su pogodni za dobijanje
ekstrakata u skladu sa principima održivog razvoja. Inovativni fizicki tretmani kao netermalni
tretman plazmom mogu obezbediti bolju ekstrakciju i mikrobiološku stabilnost posebno vodenih
ekstrakata. Takode, fermentacije bakterijama, kao što je Ligilactobacillus salivarius, mogu da
metabolišu jedinjenja prisutna u ekstraktima i biotransformišu glikozide, tanine i lignane u biološki
aktivnije oblike.
U radu smo ispitivali rast i antioksidativnu aktivnost vodenih ekstrakata cveta koprive dobijenih uz
tretman netermalnom plazmom i potom fermentaciju sa L. salivarius. Ispitali smo rast L. salivarius
u medijumima sa razlicitim udelom vodenog ekstrata cveta koprive i Man Rogosa Šarp (MRS) bujona
koji je koristi kao standardni mikrobiološki medijum za kultivaciju bakterija mlecne kiseline.
Antioksidativna aktivnost dobijenih ekstrakata je ispitana DPPH metodom, pre i nakon fermentacije.
L. salivarius je pokazao najbolji rast (11 log CFU/ml) u ekstraktima obogacenim sa 25% MRS. Potom
je je ispitivan rast L. salivarius u plazma tretiranom vodenom ekstraktu koprive sa 25% MRS. Nakon
tretmana netermalnom plazmom od 3 min i naknadne fermentacije ovih ekstrakata, postignut je rast
od 9 log CFU/ml i porast antioksidativne aktivnosti od 27% DPPH metodom. Uocen je pozitivan
kombinovani efekat netermalne plazme i fermentacije na povecanje antioksidativne aktivnosti,
posebno pri kracim tretmanima netermalnom plazmom. Rezultati rada ukazuju na mogucnost
primene zelenih tretmana i fermentacije za dobijanje proizvoda ekstrakta cveta koprive obogacenih
sa L. salivarius. Plazma tretman može uticati i na mikrobiološki sastav vodenih ekstrakata, kao
važnog aspekta za primenu u prehrambenoj i farmaceutskoj industriji.",
publisher = "Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi = National Society of Processing and Energy in Agriculture",
journal = "Knjiga sažetaka = Book of Abstracts / XXXVI Naučno-stručni skup Procesna tehnika i energetika u poljoprivredi - PTEP 2024 = XXXVI Scientific - Professional Conference Processing and Energy in Agriculture - PTEP 2024, Tara, Hotel Omorika, 15 – 18. April 2024",
title = "PLAZMA AKTIVIRANI VODENI EKSTRAKATI CVETA KOPRIVE KAO SUPSTRAT ZA GAJENJE BIOMASE LIGILACTOBACILLUS SALIVARIUS",
pages = "6",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7446"
}
Bogdanović, M., Mladenović, D., Grbić, J., Mladenović, M., Lazović, S.,& Đukić-Vuković, A.. (2024). PLAZMA AKTIVIRANI VODENI EKSTRAKATI CVETA KOPRIVE KAO SUPSTRAT ZA GAJENJE BIOMASE LIGILACTOBACILLUS SALIVARIUS. in Knjiga sažetaka = Book of Abstracts / XXXVI Naučno-stručni skup Procesna tehnika i energetika u poljoprivredi - PTEP 2024 = XXXVI Scientific - Professional Conference Processing and Energy in Agriculture - PTEP 2024, Tara, Hotel Omorika, 15 – 18. April 2024
Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi = National Society of Processing and Energy in Agriculture., 6.
https://hdl.handle.net/21.15107/rcub_technorep_7446
Bogdanović M, Mladenović D, Grbić J, Mladenović M, Lazović S, Đukić-Vuković A. PLAZMA AKTIVIRANI VODENI EKSTRAKATI CVETA KOPRIVE KAO SUPSTRAT ZA GAJENJE BIOMASE LIGILACTOBACILLUS SALIVARIUS. in Knjiga sažetaka = Book of Abstracts / XXXVI Naučno-stručni skup Procesna tehnika i energetika u poljoprivredi - PTEP 2024 = XXXVI Scientific - Professional Conference Processing and Energy in Agriculture - PTEP 2024, Tara, Hotel Omorika, 15 – 18. April 2024. 2024;:6.
https://hdl.handle.net/21.15107/rcub_technorep_7446 .
Bogdanović, Mihajlo, Mladenović, Dragana, Grbić, Jovana, Mladenović, Mihailo, Lazović, Saša, Đukić-Vuković, Aleksandra, "PLAZMA AKTIVIRANI VODENI EKSTRAKATI CVETA KOPRIVE KAO SUPSTRAT ZA GAJENJE BIOMASE LIGILACTOBACILLUS SALIVARIUS" in Knjiga sažetaka = Book of Abstracts / XXXVI Naučno-stručni skup Procesna tehnika i energetika u poljoprivredi - PTEP 2024 = XXXVI Scientific - Professional Conference Processing and Energy in Agriculture - PTEP 2024, Tara, Hotel Omorika, 15 – 18. April 2024 (2024):6,
https://hdl.handle.net/21.15107/rcub_technorep_7446 .

Intraoral administration of probiotics and postbiotics: An overview of microorganisms and formulation strategies

Bogdanović, Mihajlo; Mladenović, Dragana; Mojović, Ljiljana; Đuriš, Jelena; Đukić-Vuković, Aleksandra

(Faculty of Pharmaceutical Sciences of the University of São Paulo, 2024)

TY  - JOUR
AU  - Bogdanović, Mihajlo
AU  - Mladenović, Dragana
AU  - Mojović, Ljiljana
AU  - Đuriš, Jelena
AU  - Đukić-Vuković, Aleksandra
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7346
AB  - The last decade provided significant advances in the understanding of microbiota and its role in human health. Probiotics are live microorganisms with proven benefits for the host and were mostly studied in the context of gut health, but they can also confer significant benefits for oral health, mainly in the treatment of gingivitis. Postbiotics are cell-free extracts and metabolites of microorganisms which can provide additional preventive and therapeutic value for human health. This opens opportunities for new preventive or therapeutic formulations for oral administration. The microorganisms that colonize the oral cavity, their role in oral health and disease, as well as the probiotics and postbiotics which could have beneficial effects in this complex environment were discussed. The aim of this study was to review, analyse and discuss novel probiotic and postbiotic formulations intended for oral administration that could be of great preventive and therapeutic importance. A special attention has been put on the formulation of the pharmaceutical dosage forms that are expected to provide new benefits for the patients and technological advantages relevant for industry. An adequate dosage form could significantly enhance the efficiency of these products.
PB  - Faculty of Pharmaceutical Sciences of the University of São Paulo
T2  - Brazilian Journal of Pharmaceutical Sciences
T1  - Intraoral administration of probiotics and postbiotics: An overview of microorganisms and formulation strategies
SP  - e23272
VL  - 60
DO  - 10.1590/s2175-97902024e23272
ER  - 
@article{
author = "Bogdanović, Mihajlo and Mladenović, Dragana and Mojović, Ljiljana and Đuriš, Jelena and Đukić-Vuković, Aleksandra",
year = "2024",
abstract = "The last decade provided significant advances in the understanding of microbiota and its role in human health. Probiotics are live microorganisms with proven benefits for the host and were mostly studied in the context of gut health, but they can also confer significant benefits for oral health, mainly in the treatment of gingivitis. Postbiotics are cell-free extracts and metabolites of microorganisms which can provide additional preventive and therapeutic value for human health. This opens opportunities for new preventive or therapeutic formulations for oral administration. The microorganisms that colonize the oral cavity, their role in oral health and disease, as well as the probiotics and postbiotics which could have beneficial effects in this complex environment were discussed. The aim of this study was to review, analyse and discuss novel probiotic and postbiotic formulations intended for oral administration that could be of great preventive and therapeutic importance. A special attention has been put on the formulation of the pharmaceutical dosage forms that are expected to provide new benefits for the patients and technological advantages relevant for industry. An adequate dosage form could significantly enhance the efficiency of these products.",
publisher = "Faculty of Pharmaceutical Sciences of the University of São Paulo",
journal = "Brazilian Journal of Pharmaceutical Sciences",
title = "Intraoral administration of probiotics and postbiotics: An overview of microorganisms and formulation strategies",
pages = "e23272",
volume = "60",
doi = "10.1590/s2175-97902024e23272"
}
Bogdanović, M., Mladenović, D., Mojović, L., Đuriš, J.,& Đukić-Vuković, A.. (2024). Intraoral administration of probiotics and postbiotics: An overview of microorganisms and formulation strategies. in Brazilian Journal of Pharmaceutical Sciences
Faculty of Pharmaceutical Sciences of the University of São Paulo., 60, e23272.
https://doi.org/10.1590/s2175-97902024e23272
Bogdanović M, Mladenović D, Mojović L, Đuriš J, Đukić-Vuković A. Intraoral administration of probiotics and postbiotics: An overview of microorganisms and formulation strategies. in Brazilian Journal of Pharmaceutical Sciences. 2024;60:e23272.
doi:10.1590/s2175-97902024e23272 .
Bogdanović, Mihajlo, Mladenović, Dragana, Mojović, Ljiljana, Đuriš, Jelena, Đukić-Vuković, Aleksandra, "Intraoral administration of probiotics and postbiotics: An overview of microorganisms and formulation strategies" in Brazilian Journal of Pharmaceutical Sciences, 60 (2024):e23272,
https://doi.org/10.1590/s2175-97902024e23272 . .

Effect of combined non-thermal plasma/Fenton treatment on lignocellulose degradation in corn stalks

Grbić, Jovana; Mladenović, Dragana; Pavlović, Stefan; Veljović, Đorđe; Lazović, Saša; Đukić-Vuković, Aleksandra

(University of East Sarajevo Faculty of Technology, 2023)

TY  - CONF
AU  - Grbić, Jovana
AU  - Mladenović, Dragana
AU  - Pavlović, Stefan
AU  - Veljović, Đorđe
AU  - Lazović, Saša
AU  - Đukić-Vuković, Aleksandra
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6344
AB  - Corn is one of the world´s most commonly cultivated crops.A major part of the plant remaining after harvesting is the corn stalk.This corn residueis rich in carbohydrates and could be suitable for the fermentative production of numerous value-added products. The corn stalk’s complex structure, recalcitrance deriving from lignin, and high crystallinity and polymerization degree of cellulose prevent conventional pretreatment techniques to separate it efficiently into elementary fractions. Therefore, it is usually processed by burning directly on the ground or landfilling.It seldom can be used for composting or combusted for heat generation. In this study, different advanced oxidation processes were used for the treatment of corn stalks to enable more sustainable valorization of biomass by enzymatic hydrolysis. The effects of non-thermal plasma treatment, treatment with Fenton reagent, and combined non-thermal plasma/Fenton treatment on lignocellulose degradation and biomass digestibility were monitored. Treatment efficacy in terms of degradation was assessed by determininglignincontent. Structural and textural properties of treated biomass were analyzed using FTIR analysis and mercury intrusion porosimetry (MIP).The carbohydrate digestibility estimation was based on hexose and pentose content in hydrolyzed samples. Applied treatments showed success in breaking complex lignocellulose structures. The delignification rates for the non-thermal plasma treated sample, thesample treated only with the Fenton reagent, and the combined non-thermal plasma/Fenton treated sample were 19%, 28.7%, and 53%, respectively. Selectivity towards lignin increased with prolonging the non-thermal plasma treatmentorthe addition of the Fenton reagent.To achieve a delignification rateof 53% by using only non-thermal plasma, treatment should last at least 60 minutes. When the Fenton reagent is added, the same result is obtained with a halved duration of the plasma treatment. Under these conditions, pore size diameter in treated biomass increased, resulting in enhanced biomass digestibility with 2.25 times higher hexose yield compared to the untreated sample. This is a significant step forward in developing sustainable treatments for lignocellulosic biomass, which is especially important in biorefinery processes.
PB  - University of East Sarajevo Faculty of Technology
C3  - Book of abstracts VIII International congress Engineering, environment and materials in process industry
T1  - Effect of combined non-thermal plasma/Fenton treatment on lignocellulose degradation in corn stalks
SP  - 154
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6344
ER  - 
@conference{
author = "Grbić, Jovana and Mladenović, Dragana and Pavlović, Stefan and Veljović, Đorđe and Lazović, Saša and Đukić-Vuković, Aleksandra",
year = "2023",
abstract = "Corn is one of the world´s most commonly cultivated crops.A major part of the plant remaining after harvesting is the corn stalk.This corn residueis rich in carbohydrates and could be suitable for the fermentative production of numerous value-added products. The corn stalk’s complex structure, recalcitrance deriving from lignin, and high crystallinity and polymerization degree of cellulose prevent conventional pretreatment techniques to separate it efficiently into elementary fractions. Therefore, it is usually processed by burning directly on the ground or landfilling.It seldom can be used for composting or combusted for heat generation. In this study, different advanced oxidation processes were used for the treatment of corn stalks to enable more sustainable valorization of biomass by enzymatic hydrolysis. The effects of non-thermal plasma treatment, treatment with Fenton reagent, and combined non-thermal plasma/Fenton treatment on lignocellulose degradation and biomass digestibility were monitored. Treatment efficacy in terms of degradation was assessed by determininglignincontent. Structural and textural properties of treated biomass were analyzed using FTIR analysis and mercury intrusion porosimetry (MIP).The carbohydrate digestibility estimation was based on hexose and pentose content in hydrolyzed samples. Applied treatments showed success in breaking complex lignocellulose structures. The delignification rates for the non-thermal plasma treated sample, thesample treated only with the Fenton reagent, and the combined non-thermal plasma/Fenton treated sample were 19%, 28.7%, and 53%, respectively. Selectivity towards lignin increased with prolonging the non-thermal plasma treatmentorthe addition of the Fenton reagent.To achieve a delignification rateof 53% by using only non-thermal plasma, treatment should last at least 60 minutes. When the Fenton reagent is added, the same result is obtained with a halved duration of the plasma treatment. Under these conditions, pore size diameter in treated biomass increased, resulting in enhanced biomass digestibility with 2.25 times higher hexose yield compared to the untreated sample. This is a significant step forward in developing sustainable treatments for lignocellulosic biomass, which is especially important in biorefinery processes.",
publisher = "University of East Sarajevo Faculty of Technology",
journal = "Book of abstracts VIII International congress Engineering, environment and materials in process industry",
title = "Effect of combined non-thermal plasma/Fenton treatment on lignocellulose degradation in corn stalks",
pages = "154",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6344"
}
Grbić, J., Mladenović, D., Pavlović, S., Veljović, Đ., Lazović, S.,& Đukić-Vuković, A.. (2023). Effect of combined non-thermal plasma/Fenton treatment on lignocellulose degradation in corn stalks. in Book of abstracts VIII International congress Engineering, environment and materials in process industry
University of East Sarajevo Faculty of Technology., 154.
https://hdl.handle.net/21.15107/rcub_technorep_6344
Grbić J, Mladenović D, Pavlović S, Veljović Đ, Lazović S, Đukić-Vuković A. Effect of combined non-thermal plasma/Fenton treatment on lignocellulose degradation in corn stalks. in Book of abstracts VIII International congress Engineering, environment and materials in process industry. 2023;:154.
https://hdl.handle.net/21.15107/rcub_technorep_6344 .
Grbić, Jovana, Mladenović, Dragana, Pavlović, Stefan, Veljović, Đorđe, Lazović, Saša, Đukić-Vuković, Aleksandra, "Effect of combined non-thermal plasma/Fenton treatment on lignocellulose degradation in corn stalks" in Book of abstracts VIII International congress Engineering, environment and materials in process industry (2023):154,
https://hdl.handle.net/21.15107/rcub_technorep_6344 .

Non-thermal plasma as a pretreatment for extraction of herbal tea by-products

Grbić, Jovana; Bogdanović, Mihajlo; Mladenović, Dragana; Lazović, Saša; Mojović, Ljiljana; Đukić-Vuković, Aleksandra

(National Society of Processing and Energy in Agriculture, 2023)

TY  - JOUR
AU  - Grbić, Jovana
AU  - Bogdanović, Mihajlo
AU  - Mladenović, Dragana
AU  - Lazović, Saša
AU  - Mojović, Ljiljana
AU  - Đukić-Vuković, Aleksandra
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7139
AB  - Traditional extraction methods involve the use of polar solvents and/or high temperatures. Both are often energy or time-consuming and require additional purification steps. This compromises the quality of the extracts obtained. Non-thermal plasma pre-treatment for extraction is a promising technique that could increase the sustainability of the process while following the principles of hurdle technology. In this work, we investigated the effects of plasma treatment on the aqueous extraction of antioxidant compounds from herbal tea by-products. Stinging nettle, hoary willowherb, and St. John’s Wort extracts were treated with plasma at different time intervals. The extracts obtained were analyzed for their antioxidant capacity and total phenolic content. Their potential use as growth media for Ligilactobacillus sp. was also investigated. Hoary willowherb extracts showed the highest total phenolic content and antiox-idant activity. While plasma treatment slightly decreased the antioxidant capacity of these extracts, it improved microbial growth. In contrast to hoary willowherb, the antioxidant capacity of stinging nettle extracts’ was not affected by plasma treatment. These extracts were also better for bacterial growth. When added to 25% v/v MRS broth, stinging nettle extracts achieved a growth rate similar to that of pure MRS broth (109 CFU/ml), demonstrating their potential for microbial biomass production and food and feed supplemen-tation.
AB  - Antioksidansi imaju važnu ulogu u sprečavanju oksidacije masnih kiselina prisutnih u prehrambenim proizvodima, čime značajno produžavaju njihov rok trajanja. Zbog nepovoljnog uticaja pojedinih sintetskih antioksidanasa na zdravlje ljudi, sve je češća upotreba antioksidanasa dobijenih iz prirodnih izvora. Antioksidansi se iz biljnih izvora izoluju ekstrakcijom. Tradicionalne metode ekstrakcije podrazumevaju upotrebu polarnih rastvarača, uz primenu visokih temperatura. Ovi procesi su često energetski nepovoljni, dugo traju i zahtevaju dodatne korake prečišćavanja, kako bi se uklonili toksični rastvarači. Izolovanje biološki aktivnih jedinjenja primenom gasne netermalne plazme kao pretretmana bi moglo da poveća održivost ovog procesa. Osim toga, kombinacijom zelenih rastvarača, poput vode, sa tretmanom plazmom, dobijanje prirodnih antioksidanasa bi bilo u skladu sa tzv. „hardl“ tehnologijom i principima cirkularne ekonomije, kojima se teži u prehrambenoj industriji. Predmet ovog rada je ispitivanje mogućnosti dobijanja prirodnih anti-oksidanasa kombinovanom ekstrakcijom nusproizvoda proizvodnje biljnih čajeva. Vodeni ekstrakti koprive, svilovine i kantariona tre-tirani su plazmom u različitim vremenskim intervalima. Analizirana je antioksidativna aktivnost dobijenih ekstrakata, kao i ukupni sadržaj fenola. Takođe, ispitivana je i mogućnost upotrebe ovih ekstrakata kao fermentacionog medijuma. Najveća antioksidativna aktivnost i najviši sadržaj ukupnih fenola ostvareni su u ekstraktima svilovine. Ove vrednosti su blago opadale sa povećanjem dužine tretmana plazmom, dok je rast bakterija bio poboljšan. Ekstrakti koprive su posedovali značajno manju antioksidativnu aktivnost i niži sadržaj ukupnih fenola od ekstrakata svilovine. Međutim, ovi ekstrakti su bili pogodniji za gajenje bakterija. Dodavanjem 25% MRS bujona u ekstrakte koprive, postignut je približno isti rast Ligilactobacillus salivarius kao u čistom MRS bujonu.
PB  - National Society of Processing and Energy in Agriculture
T2  - Journal on Processing and Energy in Agriculture
T1  - Non-thermal plasma as a pretreatment for extraction of herbal tea by-products
T1  - Ekstrakcija sporednih proizvoda prerade biljnih čajeva uz pretretman gasnom plazmom
EP  - 52
IS  - 1
SP  - 47
VL  - 27
DO  - 10.5937/jpea26-44994
ER  - 
@article{
author = "Grbić, Jovana and Bogdanović, Mihajlo and Mladenović, Dragana and Lazović, Saša and Mojović, Ljiljana and Đukić-Vuković, Aleksandra",
year = "2023",
abstract = "Traditional extraction methods involve the use of polar solvents and/or high temperatures. Both are often energy or time-consuming and require additional purification steps. This compromises the quality of the extracts obtained. Non-thermal plasma pre-treatment for extraction is a promising technique that could increase the sustainability of the process while following the principles of hurdle technology. In this work, we investigated the effects of plasma treatment on the aqueous extraction of antioxidant compounds from herbal tea by-products. Stinging nettle, hoary willowherb, and St. John’s Wort extracts were treated with plasma at different time intervals. The extracts obtained were analyzed for their antioxidant capacity and total phenolic content. Their potential use as growth media for Ligilactobacillus sp. was also investigated. Hoary willowherb extracts showed the highest total phenolic content and antiox-idant activity. While plasma treatment slightly decreased the antioxidant capacity of these extracts, it improved microbial growth. In contrast to hoary willowherb, the antioxidant capacity of stinging nettle extracts’ was not affected by plasma treatment. These extracts were also better for bacterial growth. When added to 25% v/v MRS broth, stinging nettle extracts achieved a growth rate similar to that of pure MRS broth (109 CFU/ml), demonstrating their potential for microbial biomass production and food and feed supplemen-tation., Antioksidansi imaju važnu ulogu u sprečavanju oksidacije masnih kiselina prisutnih u prehrambenim proizvodima, čime značajno produžavaju njihov rok trajanja. Zbog nepovoljnog uticaja pojedinih sintetskih antioksidanasa na zdravlje ljudi, sve je češća upotreba antioksidanasa dobijenih iz prirodnih izvora. Antioksidansi se iz biljnih izvora izoluju ekstrakcijom. Tradicionalne metode ekstrakcije podrazumevaju upotrebu polarnih rastvarača, uz primenu visokih temperatura. Ovi procesi su često energetski nepovoljni, dugo traju i zahtevaju dodatne korake prečišćavanja, kako bi se uklonili toksični rastvarači. Izolovanje biološki aktivnih jedinjenja primenom gasne netermalne plazme kao pretretmana bi moglo da poveća održivost ovog procesa. Osim toga, kombinacijom zelenih rastvarača, poput vode, sa tretmanom plazmom, dobijanje prirodnih antioksidanasa bi bilo u skladu sa tzv. „hardl“ tehnologijom i principima cirkularne ekonomije, kojima se teži u prehrambenoj industriji. Predmet ovog rada je ispitivanje mogućnosti dobijanja prirodnih anti-oksidanasa kombinovanom ekstrakcijom nusproizvoda proizvodnje biljnih čajeva. Vodeni ekstrakti koprive, svilovine i kantariona tre-tirani su plazmom u različitim vremenskim intervalima. Analizirana je antioksidativna aktivnost dobijenih ekstrakata, kao i ukupni sadržaj fenola. Takođe, ispitivana je i mogućnost upotrebe ovih ekstrakata kao fermentacionog medijuma. Najveća antioksidativna aktivnost i najviši sadržaj ukupnih fenola ostvareni su u ekstraktima svilovine. Ove vrednosti su blago opadale sa povećanjem dužine tretmana plazmom, dok je rast bakterija bio poboljšan. Ekstrakti koprive su posedovali značajno manju antioksidativnu aktivnost i niži sadržaj ukupnih fenola od ekstrakata svilovine. Međutim, ovi ekstrakti su bili pogodniji za gajenje bakterija. Dodavanjem 25% MRS bujona u ekstrakte koprive, postignut je približno isti rast Ligilactobacillus salivarius kao u čistom MRS bujonu.",
publisher = "National Society of Processing and Energy in Agriculture",
journal = "Journal on Processing and Energy in Agriculture",
title = "Non-thermal plasma as a pretreatment for extraction of herbal tea by-products, Ekstrakcija sporednih proizvoda prerade biljnih čajeva uz pretretman gasnom plazmom",
pages = "52-47",
number = "1",
volume = "27",
doi = "10.5937/jpea26-44994"
}
Grbić, J., Bogdanović, M., Mladenović, D., Lazović, S., Mojović, L.,& Đukić-Vuković, A.. (2023). Non-thermal plasma as a pretreatment for extraction of herbal tea by-products. in Journal on Processing and Energy in Agriculture
National Society of Processing and Energy in Agriculture., 27(1), 47-52.
https://doi.org/10.5937/jpea26-44994
Grbić J, Bogdanović M, Mladenović D, Lazović S, Mojović L, Đukić-Vuković A. Non-thermal plasma as a pretreatment for extraction of herbal tea by-products. in Journal on Processing and Energy in Agriculture. 2023;27(1):47-52.
doi:10.5937/jpea26-44994 .
Grbić, Jovana, Bogdanović, Mihajlo, Mladenović, Dragana, Lazović, Saša, Mojović, Ljiljana, Đukić-Vuković, Aleksandra, "Non-thermal plasma as a pretreatment for extraction of herbal tea by-products" in Journal on Processing and Energy in Agriculture, 27, no. 1 (2023):47-52,
https://doi.org/10.5937/jpea26-44994 . .

Different treatments of lignocellulosic biomass for enhanced delignification and enzymatic hydrolysis

Grbić, Jovana; Mladenović, Dragana; Žilović, Jana; Lazović, Saša; Mijin, Dušan; Đukić-Vuković, Aleksandra

(Belgrade : University, Faculty of Technology and Metallurgy, 2023)

TY  - CONF
AU  - Grbić, Jovana
AU  - Mladenović, Dragana
AU  - Žilović, Jana
AU  - Lazović, Saša
AU  - Mijin, Dušan
AU  - Đukić-Vuković, Aleksandra
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6942
AB  - Lignocellulosic biomass (LCB) valorization is a fundamental step toward circular
bioeconomy. The complex structure of lignocellulose must be disrupted to conduct thorough valorization. Conventional methods often lack sustainability, by having high energy requirements, or a harmful impact on the environment. To overcome these impediments, novel tenable methods must be developed. This work compares different green solvent-based treatments of LCB, regarding the delignification rate and the enzymatic hydrolysis efficiency. Due to abundance and high availability, corn residues were used as model substrates. Deep eutectic solvent (DES), microwave-assisted alkaline treatment, combined non-thermal
plasma/Fenton, and non-thermal plasma/alkaline treatment were selected as green methods for LCB treatment, having higher selectivity favoring lignin degradation and milder reaction conditions. Treated biomass was analyzed for acetyl bromide soluble lignin content and subjected to enzymatic hydrolysis with Cellic® CTec2, followed by hexose and pentose content determination. The best results regarding lignin content were obtained with DES, when the choline chloride to lactic acid ratio was 1:10, achieving a delignification rate of 86%. Combined plasma/alkaline treatment for 10 minutes decreased lignin content by 77%, while microwave-assisted alkaline treatment decreased it by 74% for only one minute. When combined with the Fenton reagent, a 30-minute-long plasma treatment reduced lignin content by 53%. Moreover, FTIR spectroscopy confirmed partial depolymerization of lignin, making it more suitable for further application. Each treatment intensified LCB decomposition and facilitated enzyme penetration, increasing hexose yield by 2.3-5.6 times, and pentose yield by 1.7-8.0 times compared to the untreated biomass. Methods implemented in this work
contributed to feasible biomass delignification. Coupling alkaline hydrogen peroxide with microwave irradiation or non-thermal plasma or applying reusable DES could significantly promote proficient biotechnological exploitation of lignin and carbohydrate fractions in a time-saving and cost-effective way. The possibility of biotechnological production of natural antioxidants and natural aromatic compounds should be particularly underlined.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - Different treatments of lignocellulosic biomass for enhanced delignification and enzymatic hydrolysis
SP  - 62
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6942
ER  - 
@conference{
author = "Grbić, Jovana and Mladenović, Dragana and Žilović, Jana and Lazović, Saša and Mijin, Dušan and Đukić-Vuković, Aleksandra",
year = "2023",
abstract = "Lignocellulosic biomass (LCB) valorization is a fundamental step toward circular
bioeconomy. The complex structure of lignocellulose must be disrupted to conduct thorough valorization. Conventional methods often lack sustainability, by having high energy requirements, or a harmful impact on the environment. To overcome these impediments, novel tenable methods must be developed. This work compares different green solvent-based treatments of LCB, regarding the delignification rate and the enzymatic hydrolysis efficiency. Due to abundance and high availability, corn residues were used as model substrates. Deep eutectic solvent (DES), microwave-assisted alkaline treatment, combined non-thermal
plasma/Fenton, and non-thermal plasma/alkaline treatment were selected as green methods for LCB treatment, having higher selectivity favoring lignin degradation and milder reaction conditions. Treated biomass was analyzed for acetyl bromide soluble lignin content and subjected to enzymatic hydrolysis with Cellic® CTec2, followed by hexose and pentose content determination. The best results regarding lignin content were obtained with DES, when the choline chloride to lactic acid ratio was 1:10, achieving a delignification rate of 86%. Combined plasma/alkaline treatment for 10 minutes decreased lignin content by 77%, while microwave-assisted alkaline treatment decreased it by 74% for only one minute. When combined with the Fenton reagent, a 30-minute-long plasma treatment reduced lignin content by 53%. Moreover, FTIR spectroscopy confirmed partial depolymerization of lignin, making it more suitable for further application. Each treatment intensified LCB decomposition and facilitated enzyme penetration, increasing hexose yield by 2.3-5.6 times, and pentose yield by 1.7-8.0 times compared to the untreated biomass. Methods implemented in this work
contributed to feasible biomass delignification. Coupling alkaline hydrogen peroxide with microwave irradiation or non-thermal plasma or applying reusable DES could significantly promote proficient biotechnological exploitation of lignin and carbohydrate fractions in a time-saving and cost-effective way. The possibility of biotechnological production of natural antioxidants and natural aromatic compounds should be particularly underlined.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "Different treatments of lignocellulosic biomass for enhanced delignification and enzymatic hydrolysis",
pages = "62",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6942"
}
Grbić, J., Mladenović, D., Žilović, J., Lazović, S., Mijin, D.,& Đukić-Vuković, A.. (2023). Different treatments of lignocellulosic biomass for enhanced delignification and enzymatic hydrolysis. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 62.
https://hdl.handle.net/21.15107/rcub_technorep_6942
Grbić J, Mladenović D, Žilović J, Lazović S, Mijin D, Đukić-Vuković A. Different treatments of lignocellulosic biomass for enhanced delignification and enzymatic hydrolysis. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:62.
https://hdl.handle.net/21.15107/rcub_technorep_6942 .
Grbić, Jovana, Mladenović, Dragana, Žilović, Jana, Lazović, Saša, Mijin, Dušan, Đukić-Vuković, Aleksandra, "Different treatments of lignocellulosic biomass for enhanced delignification and enzymatic hydrolysis" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):62,
https://hdl.handle.net/21.15107/rcub_technorep_6942 .

By-products from the processing of herbs as sources of antioxidants

Mladenović, Mihailo; Grbić, Jovana; Moskovljević, Mia; Bogdanović, Mihajlo; Mladenović, Dragana; Lukić, Ivana; Đukić-Vuković, Aleksandra

(Belgrade : University, Faculty of Technology and Metallurgy, 2023)

TY  - CONF
AU  - Mladenović, Mihailo
AU  - Grbić, Jovana
AU  - Moskovljević, Mia
AU  - Bogdanović, Mihajlo
AU  - Mladenović, Dragana
AU  - Lukić, Ivana
AU  - Đukić-Vuković, Aleksandra
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6944
AB  - During the processing of herbs, a significant amount of chopped biomass by-products is left behind, often discarded and underutilized. Since they are important sources of different groups of bioactives like polyphenolics, essential oils or terpenes and structural compounds like lignocellulose, they could be exploited in fermentation by different microorganisms. However, fractionation and extractions could significantly increase the effectiveness and productivity of the valorization process. We examined the possibility of extracting antioxidants from by-products of hoary willowherb (Epilobium spp.), sage (Salvia spp.), and
basil (Ocimum spp.), as medicinal herbs with significant pharmaceutical and market value. Extracts of hoary willowherb, sage and basil residues were obtained by a conventional, Soxhlet extraction for polar compounds and supercritical carbon(IV)-oxide extraction as an unconventional extraction method for non-polar compounds. We measured the antioxidant content in the obtained extracts by the ABTS and DPPH spectrophotometric methods. In addition, we measured the total polyphenol content of the extracts by the Folin-Coicalteu
method. According to the ABTS method, the antioxidant activity in the sage extracts obtained from Soxhlet extraction is 2.2 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity in the basil extracts obtained from Soxhlet extraction is 9.1 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction, according to the DPPH method. The total polyphenol content in the hoary willowherb extracts obtained from Soxhlet extraction is 4.6 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity is greater in the extracts obtained by Soxhlet extraction than in the extracts obtained with supercritical carbon(IV)-oxide extraction. Because we examined residues from the tea processing industry, the content of easily volatile non-polar components is expected to be lower. The results confirm that valorization of these residues should be focused on the polar components and their microbial biotransformations.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - By-products from the processing of herbs as sources of antioxidants
SP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6944
ER  - 
@conference{
author = "Mladenović, Mihailo and Grbić, Jovana and Moskovljević, Mia and Bogdanović, Mihajlo and Mladenović, Dragana and Lukić, Ivana and Đukić-Vuković, Aleksandra",
year = "2023",
abstract = "During the processing of herbs, a significant amount of chopped biomass by-products is left behind, often discarded and underutilized. Since they are important sources of different groups of bioactives like polyphenolics, essential oils or terpenes and structural compounds like lignocellulose, they could be exploited in fermentation by different microorganisms. However, fractionation and extractions could significantly increase the effectiveness and productivity of the valorization process. We examined the possibility of extracting antioxidants from by-products of hoary willowherb (Epilobium spp.), sage (Salvia spp.), and
basil (Ocimum spp.), as medicinal herbs with significant pharmaceutical and market value. Extracts of hoary willowherb, sage and basil residues were obtained by a conventional, Soxhlet extraction for polar compounds and supercritical carbon(IV)-oxide extraction as an unconventional extraction method for non-polar compounds. We measured the antioxidant content in the obtained extracts by the ABTS and DPPH spectrophotometric methods. In addition, we measured the total polyphenol content of the extracts by the Folin-Coicalteu
method. According to the ABTS method, the antioxidant activity in the sage extracts obtained from Soxhlet extraction is 2.2 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity in the basil extracts obtained from Soxhlet extraction is 9.1 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction, according to the DPPH method. The total polyphenol content in the hoary willowherb extracts obtained from Soxhlet extraction is 4.6 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity is greater in the extracts obtained by Soxhlet extraction than in the extracts obtained with supercritical carbon(IV)-oxide extraction. Because we examined residues from the tea processing industry, the content of easily volatile non-polar components is expected to be lower. The results confirm that valorization of these residues should be focused on the polar components and their microbial biotransformations.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "By-products from the processing of herbs as sources of antioxidants",
pages = "20",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6944"
}
Mladenović, M., Grbić, J., Moskovljević, M., Bogdanović, M., Mladenović, D., Lukić, I.,& Đukić-Vuković, A.. (2023). By-products from the processing of herbs as sources of antioxidants. in Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 20.
https://hdl.handle.net/21.15107/rcub_technorep_6944
Mladenović M, Grbić J, Moskovljević M, Bogdanović M, Mladenović D, Lukić I, Đukić-Vuković A. By-products from the processing of herbs as sources of antioxidants. in Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:20.
https://hdl.handle.net/21.15107/rcub_technorep_6944 .
Mladenović, Mihailo, Grbić, Jovana, Moskovljević, Mia, Bogdanović, Mihajlo, Mladenović, Dragana, Lukić, Ivana, Đukić-Vuković, Aleksandra, "By-products from the processing of herbs as sources of antioxidants" in Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):20,
https://hdl.handle.net/21.15107/rcub_technorep_6944 .

Fractionation of corncob biomass towards sustainable valorization in biorefinery processes

Mladenović, Dragana; Grbić, Jovana; Đukić-Vuković, Aleksandra; Mojović, Ljiljana

(National Technical University of Athens, 2023)

TY  - CONF
AU  - Mladenović, Dragana
AU  - Grbić, Jovana
AU  - Đukić-Vuković, Aleksandra
AU  - Mojović, Ljiljana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6747
PB  - National Technical University of Athens
C3  - 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece
T1  - Fractionation of corncob biomass towards sustainable valorization in biorefinery processes
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6747
ER  - 
@conference{
author = "Mladenović, Dragana and Grbić, Jovana and Đukić-Vuković, Aleksandra and Mojović, Ljiljana",
year = "2023",
publisher = "National Technical University of Athens",
journal = "10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece",
title = "Fractionation of corncob biomass towards sustainable valorization in biorefinery processes",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6747"
}
Mladenović, D., Grbić, J., Đukić-Vuković, A.,& Mojović, L.. (2023). Fractionation of corncob biomass towards sustainable valorization in biorefinery processes. in 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece
National Technical University of Athens..
https://hdl.handle.net/21.15107/rcub_technorep_6747
Mladenović D, Grbić J, Đukić-Vuković A, Mojović L. Fractionation of corncob biomass towards sustainable valorization in biorefinery processes. in 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_6747 .
Mladenović, Dragana, Grbić, Jovana, Đukić-Vuković, Aleksandra, Mojović, Ljiljana, "Fractionation of corncob biomass towards sustainable valorization in biorefinery processes" in 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece (2023),
https://hdl.handle.net/21.15107/rcub_technorep_6747 .

Effect of non-thermal plasma treatment on antioxidative and prebiotic properties of aqueous herbal extracts

Grbić, Jovana; Bogdanović, Mihajlo; Mladenović, Dragana; Lazović, Saša; Mojović, Ljiljana; Đukić-Vuković, Aleksandra

(University of East Sarajevo Faculty of Technology, 2023)

TY  - CONF
AU  - Grbić, Jovana
AU  - Bogdanović, Mihajlo
AU  - Mladenović, Dragana
AU  - Lazović, Saša
AU  - Mojović, Ljiljana
AU  - Đukić-Vuković, Aleksandra
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6345
AB  - To provide food with a longer shelf life, synthetic antioxidants are frequently used in the food industry. Common synthetic antioxidants includebutylated hydroxyanisole(BHA), butylated hydroxytoluene (BHT), propyl gallate (PG), and tert-butylated hydroquinone (TBHQ). Since those compounds are suspected to cause some health problems, such as liver damage, there is a strong initiative to develop and utilizeantioxidantsfrom natural origins.Medicinal plants could be a good alternative source of these compounds. Besides antioxidative properties, natural compounds derived from medicinal plants often have various biological activities, like anti-inflammatory, antimicrobial, analgesic, etc.These compounds can contribute to food's functional and technological properties and interact with food microbiota. In this study, aqueous extracts of tea production residues of common nettle (Urtica dioica L.), St. John’s wort (Hypericum perforatum L.), and hoary willowherb(Epilobium parviflorum L.) were treated with non-thermal plasma. The effect of different durations of plasma treatment on the antioxidative capacity of these extracts was monitored. The fermentation of these extracts solely and extracts supplemented with MRS broth by Ligilactobacillus salivarius was examined. Antioxidative properties of plasma-treated extracts were analyzed using DPPH and ABTS methods. The total phenol content of these extracts was determined by the Folin-Ciocalteu reagent. Generally, hoary willowherb extracts were rich in total phenol content, two or three orders of magnitude richer than the other two plants. They also showed significantly higher antioxidative activity. This activity slightly decreased with prolonged plasma treatment. However, that didn’t affect bacterial growth in these extracts. It was shown that L. salivarius growth was enhanced in the pure plasma-activated extracts of hoary willowherbin comparison to extracts supplemented with MRS broth. Though, the observed effects are substrate-dependent and vary from plant to plant. The antioxidative capacity of common nettle extracts remained intact during the plasma treatment. With the addition of MRS broth (25% v/v), bacterial growth was completely unhindered, providing approximately 109 CFU/ml during the exponential phase. Moreover, the 10-minute-long plasma treatment was effective in the inactivation of microorganisms already present in herbal biomass, preventing the spoilage of treated extracts. This could be an important step up in extraction by hurdle technology, especially in the production of symbiotic preparations possessing both antioxidative and probiotic properties.
PB  - University of East Sarajevo Faculty of Technology
C3  - Book of abstracts VIII International congress Engineering, environment and materials in process industry
T1  - Effect of non-thermal plasma treatment on antioxidative and prebiotic properties of aqueous herbal extracts
SP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6345
ER  - 
@conference{
author = "Grbić, Jovana and Bogdanović, Mihajlo and Mladenović, Dragana and Lazović, Saša and Mojović, Ljiljana and Đukić-Vuković, Aleksandra",
year = "2023",
abstract = "To provide food with a longer shelf life, synthetic antioxidants are frequently used in the food industry. Common synthetic antioxidants includebutylated hydroxyanisole(BHA), butylated hydroxytoluene (BHT), propyl gallate (PG), and tert-butylated hydroquinone (TBHQ). Since those compounds are suspected to cause some health problems, such as liver damage, there is a strong initiative to develop and utilizeantioxidantsfrom natural origins.Medicinal plants could be a good alternative source of these compounds. Besides antioxidative properties, natural compounds derived from medicinal plants often have various biological activities, like anti-inflammatory, antimicrobial, analgesic, etc.These compounds can contribute to food's functional and technological properties and interact with food microbiota. In this study, aqueous extracts of tea production residues of common nettle (Urtica dioica L.), St. John’s wort (Hypericum perforatum L.), and hoary willowherb(Epilobium parviflorum L.) were treated with non-thermal plasma. The effect of different durations of plasma treatment on the antioxidative capacity of these extracts was monitored. The fermentation of these extracts solely and extracts supplemented with MRS broth by Ligilactobacillus salivarius was examined. Antioxidative properties of plasma-treated extracts were analyzed using DPPH and ABTS methods. The total phenol content of these extracts was determined by the Folin-Ciocalteu reagent. Generally, hoary willowherb extracts were rich in total phenol content, two or three orders of magnitude richer than the other two plants. They also showed significantly higher antioxidative activity. This activity slightly decreased with prolonged plasma treatment. However, that didn’t affect bacterial growth in these extracts. It was shown that L. salivarius growth was enhanced in the pure plasma-activated extracts of hoary willowherbin comparison to extracts supplemented with MRS broth. Though, the observed effects are substrate-dependent and vary from plant to plant. The antioxidative capacity of common nettle extracts remained intact during the plasma treatment. With the addition of MRS broth (25% v/v), bacterial growth was completely unhindered, providing approximately 109 CFU/ml during the exponential phase. Moreover, the 10-minute-long plasma treatment was effective in the inactivation of microorganisms already present in herbal biomass, preventing the spoilage of treated extracts. This could be an important step up in extraction by hurdle technology, especially in the production of symbiotic preparations possessing both antioxidative and probiotic properties.",
publisher = "University of East Sarajevo Faculty of Technology",
journal = "Book of abstracts VIII International congress Engineering, environment and materials in process industry",
title = "Effect of non-thermal plasma treatment on antioxidative and prebiotic properties of aqueous herbal extracts",
pages = "155",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6345"
}
Grbić, J., Bogdanović, M., Mladenović, D., Lazović, S., Mojović, L.,& Đukić-Vuković, A.. (2023). Effect of non-thermal plasma treatment on antioxidative and prebiotic properties of aqueous herbal extracts. in Book of abstracts VIII International congress Engineering, environment and materials in process industry
University of East Sarajevo Faculty of Technology., 155.
https://hdl.handle.net/21.15107/rcub_technorep_6345
Grbić J, Bogdanović M, Mladenović D, Lazović S, Mojović L, Đukić-Vuković A. Effect of non-thermal plasma treatment on antioxidative and prebiotic properties of aqueous herbal extracts. in Book of abstracts VIII International congress Engineering, environment and materials in process industry. 2023;:155.
https://hdl.handle.net/21.15107/rcub_technorep_6345 .
Grbić, Jovana, Bogdanović, Mihajlo, Mladenović, Dragana, Lazović, Saša, Mojović, Ljiljana, Đukić-Vuković, Aleksandra, "Effect of non-thermal plasma treatment on antioxidative and prebiotic properties of aqueous herbal extracts" in Book of abstracts VIII International congress Engineering, environment and materials in process industry (2023):155,
https://hdl.handle.net/21.15107/rcub_technorep_6345 .