@article{
author = "de Barros, Dragana P. C. and Fonseca, Luis P. and Fernandes, P. and Cabral, Joaquim M. S. and Mojović, Ljiljana",
year = "2009",
abstract = "The main objective of this work was to study the enzymatic synthesis of short chain ethyl esters, a group of relevant aroma molecules, by Fusarium solani pisi cutinase in an organic solvent media (iso-octane), and to assess the influence of different parameters on the reaction yield. Cutinase displayed high initial esterification rates in iso-octane. which amounted to 1.15 mu mol min(-1) mg(-1) for ethyl butyrate (C-4 acid chain) and 1.06 mu mol min(-1) mg(-1) for ethyl valerate (C-S acid chain). High product yields, 84% for ethyl butyrate and 96% for ethyl valerate, were observed after 6 h of reaction, for an initial equimolar concentration of substrates (0.1 M). The highest product yield (97%) was observed for ethyl caproate (C-6) synthesis, a compound which is a part of natural apple and pineapple flavour, for an alcohol:acid molar ratio of 2 (0.2 M ethanol concentration). Cutinase affinity for short chain length carboxylic acids (C-4-C-6) in ester synthesis in iso-octane confirmed previous observations in reversed micellar system.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Molecular Catalysis B-Enzymatic",
title = "Biosynthesis of ethyl caproate and other short ethyl esters catalyzed by cutinase in organic solvent",
pages = "185-178",
number = "3-4",
volume = "60",
doi = "10.1016/j.molcatb.2009.05.004"
}