TY  - JOUR
AU  - Stojkovska, Jasmina
AU  - Bugarski, Branko
AU  - Obradović, Bojana
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1725
AB  - Alginate hydrogels in forms of discs and packed beds of microbeads (similar to 800 mu m) were tested in a novel bioreactor at 10% strain using two regimes: at a loading rate of 337.5 mu m/s and at sequential increments of 50 mu m displacement every 30 min. Compressive strength increased with the increase in alginate concentration (1.5 vs. 2% w/w) and the content of guluronic residues (38.5 vs. 67%). Packed beds of microbeads exhibited significantly higher (similar to 1.5-3.4 fold) compression moduli than the respective discs indicating the effects of gel form and entrapped water. Short-term cultivation of microbeads with immobilized bovine calf chondrocytes (1.5% w/w, 33 x 10(6) cells/ml) under biomimetic conditions (dynamic compression: 1 h on/1 h off, 0.42 Hz, 10% strain) resulted in cell proliferation and bed compaction, so that the compression modulus slightly increased. Thus, the novel bioreactor demonstrated advantages in evaluation of biomaterial properties and cell-biomaterial interactions under in vivo-like settings.
PB  - Springer, Dordrecht
T2  - Journal of Materials Science-Materials in Medicine
T1  - Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering
EP  - 2879
IS  - 10
SP  - 2869
VL  - 21
DO  - 10.1007/s10856-010-4135-0
ER  - 

@article{
author = "Stojkovska, Jasmina and Bugarski, Branko and Obradović, Bojana",
year = "2010",
abstract = "Alginate hydrogels in forms of discs and packed beds of microbeads (similar to 800 mu m) were tested in a novel bioreactor at 10% strain using two regimes: at a loading rate of 337.5 mu m/s and at sequential increments of 50 mu m displacement every 30 min. Compressive strength increased with the increase in alginate concentration (1.5 vs. 2% w/w) and the content of guluronic residues (38.5 vs. 67%). Packed beds of microbeads exhibited significantly higher (similar to 1.5-3.4 fold) compression moduli than the respective discs indicating the effects of gel form and entrapped water. Short-term cultivation of microbeads with immobilized bovine calf chondrocytes (1.5% w/w, 33 x 10(6) cells/ml) under biomimetic conditions (dynamic compression: 1 h on/1 h off, 0.42 Hz, 10% strain) resulted in cell proliferation and bed compaction, so that the compression modulus slightly increased. Thus, the novel bioreactor demonstrated advantages in evaluation of biomaterial properties and cell-biomaterial interactions under in vivo-like settings.",
publisher = "Springer, Dordrecht",
journal = "Journal of Materials Science-Materials in Medicine",
title = "Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering",
pages = "2879-2869",
number = "10",
volume = "21",
doi = "10.1007/s10856-010-4135-0"
}

Stojkovska, J., Bugarski, B.,& Obradović, B.. (2010). Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering. in Journal of Materials Science-Materials in Medicine
Springer, Dordrecht., 21(10), 2869-2879.
https://doi.org/10.1007/s10856-010-4135-0

Stojkovska J, Bugarski B, Obradović B. Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering. in Journal of Materials Science-Materials in Medicine. 2010;21(10):2869-2879.
doi:10.1007/s10856-010-4135-0 .

Stojkovska, Jasmina, Bugarski, Branko, Obradović, Bojana, "Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering" in Journal of Materials Science-Materials in Medicine, 21, no. 10 (2010):2869-2879,
https://doi.org/10.1007/s10856-010-4135-0 . .