TY  - JOUR
AU  - Jovanović, Aleksandra A.
AU  - Vajić, Una-Jovana V.
AU  - Mijin, Dušan Ž.
AU  - Zdunić, Gordana M.
AU  - Šavikin, Katarina P.
AU  - Branković, Suzana
AU  - Kitić, Dušanka
AU  - Bugarski, Branko M.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5194
AB  - In the present study, polyphenol extraction from Thymus serpyllum by-product (herbal dust) in microwave reactor was optimized through varying extraction time, ethanol concentration and solid-to-solvent ratiovia determination of total polyphenol and flavonoid contents, and antioxidant activity, using response surface methodology and a central composite design. Total polyphenol and flavonoids contents, as well as antioxidant activity of the extracts were statistically significantly affected by ethanol concentration and solid-to-solvent ratio. However, extraction time had statistically significant influence only on polyphenol yield. The extraction conditions that maximized polyphenol recovery were 48% ethanol and 0.0402 g/mL solid-to-solvent ratio during 86 s of microwave-assisted extraction, and under these conditions total polyphenol content was estimated to be 57.2 mg of gallic acid equivalents (GAE)/L, while measured value was 58.1 ± 3.2 mg GAE/L. Polyphenol content (HPLC analysis), antioxidant, antimicrobial and antispasmodic potential of the extract prepared under these optimal extraction conditions were evaluated. Rosmarinic acids, luteolin 7-O-glucuronide, caffeic acid, apigenin glucuronide, 6-hydroxyluteolin 7-O-glucoside, 6,8-di-C-glucosylapigenin, and traces of chlorogenic acid were quantified in the extract (descending order of content). The selected extract inhibited 95% ± 0.9 of lipid peroxidation in β-Carotene and linoleic acid emulsion. It was more effective against tested Gram-positive bacteria than against Gram-negative and showed dose-dependent antispasmodic activity on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions. Microwave-assisted extraction could be selected as a successful technique for polyphenol extraction from wild thyme by-product. Biological activities shown in the study open the possibility of potential application of the extract in pharmaceutical, food, functional food and cosmetic products
PB  - Elsevier GmbH
T2  - Journal of Applied Research on Medicinal and Aromatic Plants
T1  - Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract
SP  - 100417
VL  - 31
DO  - 10.1016/j.jarmap.2022.100417
ER  - 

@article{
author = "Jovanović, Aleksandra A. and Vajić, Una-Jovana V. and Mijin, Dušan Ž. and Zdunić, Gordana M. and Šavikin, Katarina P. and Branković, Suzana and Kitić, Dušanka and Bugarski, Branko M.",
year = "2022",
abstract = "In the present study, polyphenol extraction from Thymus serpyllum by-product (herbal dust) in microwave reactor was optimized through varying extraction time, ethanol concentration and solid-to-solvent ratiovia determination of total polyphenol and flavonoid contents, and antioxidant activity, using response surface methodology and a central composite design. Total polyphenol and flavonoids contents, as well as antioxidant activity of the extracts were statistically significantly affected by ethanol concentration and solid-to-solvent ratio. However, extraction time had statistically significant influence only on polyphenol yield. The extraction conditions that maximized polyphenol recovery were 48% ethanol and 0.0402 g/mL solid-to-solvent ratio during 86 s of microwave-assisted extraction, and under these conditions total polyphenol content was estimated to be 57.2 mg of gallic acid equivalents (GAE)/L, while measured value was 58.1 ± 3.2 mg GAE/L. Polyphenol content (HPLC analysis), antioxidant, antimicrobial and antispasmodic potential of the extract prepared under these optimal extraction conditions were evaluated. Rosmarinic acids, luteolin 7-O-glucuronide, caffeic acid, apigenin glucuronide, 6-hydroxyluteolin 7-O-glucoside, 6,8-di-C-glucosylapigenin, and traces of chlorogenic acid were quantified in the extract (descending order of content). The selected extract inhibited 95% ± 0.9 of lipid peroxidation in β-Carotene and linoleic acid emulsion. It was more effective against tested Gram-positive bacteria than against Gram-negative and showed dose-dependent antispasmodic activity on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions. Microwave-assisted extraction could be selected as a successful technique for polyphenol extraction from wild thyme by-product. Biological activities shown in the study open the possibility of potential application of the extract in pharmaceutical, food, functional food and cosmetic products",
publisher = "Elsevier GmbH",
journal = "Journal of Applied Research on Medicinal and Aromatic Plants",
title = "Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract",
pages = "100417",
volume = "31",
doi = "10.1016/j.jarmap.2022.100417"
}

Jovanović, A. A., Vajić, U. V., Mijin, D. Ž., Zdunić, G. M., Šavikin, K. P., Branković, S., Kitić, D.,& Bugarski, B. M.. (2022). Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract. in Journal of Applied Research on Medicinal and Aromatic Plants
Elsevier GmbH., 31, 100417.
https://doi.org/10.1016/j.jarmap.2022.100417

Jovanović AA, Vajić UV, Mijin DŽ, Zdunić GM, Šavikin KP, Branković S, Kitić D, Bugarski BM. Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract. in Journal of Applied Research on Medicinal and Aromatic Plants. 2022;31:100417.
doi:10.1016/j.jarmap.2022.100417 .

Jovanović, Aleksandra A., Vajić, Una-Jovana V., Mijin, Dušan Ž., Zdunić, Gordana M., Šavikin, Katarina P., Branković, Suzana, Kitić, Dušanka, Bugarski, Branko M., "Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract" in Journal of Applied Research on Medicinal and Aromatic Plants, 31 (2022):100417,
https://doi.org/10.1016/j.jarmap.2022.100417 . .

TY  - JOUR
AU  - Tadic, Vanja M.
AU  - Zugic, Ana
AU  - Martinovic, Milica
AU  - Stankovic, Milica
AU  - Maksimović, Svetolik
AU  - Frank, Almut
AU  - Nesic, Ivana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4821
AB  - Immortelle, as rich source of chlorogenic acid and the phloroglucinol alpha-pyrone compound arzanol, possesses anti-inflammatory and antioxidant properties, affects cell regeneration, and has positive effect on many skin conditions. Hemp oil, characterized by a favorable omega-6 to omega-3 ratio, as well as an abundance of essential fatty acids and vitamin E, participates in immunoregulation and also act as an anti-inflammatory. In the present study, we examined the effect on the skin of creams and emulgels with immortelle extract and hemp oil, by comparing them to placebo samples and a non-treated control. A long-term in vivo study of biophysical skin characteristics, which lasted for 30 days, was conducted on 25 healthy human volunteers. Measured parameters were electrical capacitance of the stratum corneum, trans-epidermal water loss (TEWL), and skin pH and erythema index. Further, a sensory study was carried out in which the panelists had to choose descriptive terms for sensory attributes in questionnaire. The results showed that application of all preparations led to increase of skin hydration and TEWL reduction, while the skin was not irritated, and its normal pH was not disrupted. This study also showed importance of the carrier. Not only were emulgels described by panelists as preparations with better sensory properties, there was a significant difference between the skin hydration effect of emulgel with immortelle extract and hemp oil compared to the placebo emulgel, which was not the case with creams. Such findings indicated enhanced delivery of herbal active substances from emulgel compared to the cream.
T2  - Pharmaceutics
T1  - Enhanced Skin Performance of Emulgel vs. Cream as Systems for Topical Delivery of Herbal Actives (Immortelle Extract and Hemp Oil)
IS  - 11
VL  - 13
DO  - 10.3390/pharmaceutics13111919
ER  - 

@article{
author = "Tadic, Vanja M. and Zugic, Ana and Martinovic, Milica and Stankovic, Milica and Maksimović, Svetolik and Frank, Almut and Nesic, Ivana",
year = "2021",
abstract = "Immortelle, as rich source of chlorogenic acid and the phloroglucinol alpha-pyrone compound arzanol, possesses anti-inflammatory and antioxidant properties, affects cell regeneration, and has positive effect on many skin conditions. Hemp oil, characterized by a favorable omega-6 to omega-3 ratio, as well as an abundance of essential fatty acids and vitamin E, participates in immunoregulation and also act as an anti-inflammatory. In the present study, we examined the effect on the skin of creams and emulgels with immortelle extract and hemp oil, by comparing them to placebo samples and a non-treated control. A long-term in vivo study of biophysical skin characteristics, which lasted for 30 days, was conducted on 25 healthy human volunteers. Measured parameters were electrical capacitance of the stratum corneum, trans-epidermal water loss (TEWL), and skin pH and erythema index. Further, a sensory study was carried out in which the panelists had to choose descriptive terms for sensory attributes in questionnaire. The results showed that application of all preparations led to increase of skin hydration and TEWL reduction, while the skin was not irritated, and its normal pH was not disrupted. This study also showed importance of the carrier. Not only were emulgels described by panelists as preparations with better sensory properties, there was a significant difference between the skin hydration effect of emulgel with immortelle extract and hemp oil compared to the placebo emulgel, which was not the case with creams. Such findings indicated enhanced delivery of herbal active substances from emulgel compared to the cream.",
journal = "Pharmaceutics",
title = "Enhanced Skin Performance of Emulgel vs. Cream as Systems for Topical Delivery of Herbal Actives (Immortelle Extract and Hemp Oil)",
number = "11",
volume = "13",
doi = "10.3390/pharmaceutics13111919"
}

Tadic, V. M., Zugic, A., Martinovic, M., Stankovic, M., Maksimović, S., Frank, A.,& Nesic, I.. (2021). Enhanced Skin Performance of Emulgel vs. Cream as Systems for Topical Delivery of Herbal Actives (Immortelle Extract and Hemp Oil). in Pharmaceutics, 13(11).
https://doi.org/10.3390/pharmaceutics13111919

Tadic VM, Zugic A, Martinovic M, Stankovic M, Maksimović S, Frank A, Nesic I. Enhanced Skin Performance of Emulgel vs. Cream as Systems for Topical Delivery of Herbal Actives (Immortelle Extract and Hemp Oil). in Pharmaceutics. 2021;13(11).
doi:10.3390/pharmaceutics13111919 .

Tadic, Vanja M., Zugic, Ana, Martinovic, Milica, Stankovic, Milica, Maksimović, Svetolik, Frank, Almut, Nesic, Ivana, "Enhanced Skin Performance of Emulgel vs. Cream as Systems for Topical Delivery of Herbal Actives (Immortelle Extract and Hemp Oil)" in Pharmaceutics, 13, no. 11 (2021),
https://doi.org/10.3390/pharmaceutics13111919 . .