TY  - JOUR
AU  - Vuckovic, Nemanja
AU  - Glodovic, Nikola
AU  - Radovanović, Željko
AU  - Janaćković, Đorđe
AU  - Milasinovic, Nikola
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4943
AB  - Most chemical and physical methods employed in visualizing latent marks have shown detrimental effects on human health and, therefore, some research groups have directed their attention to the utilization of various (bio)polymers with the aforementioned purpose. Although chitosan is widely used in medicine, pharmacy, food, and drug delivery systems, there are very few studies that address this biopolymer utilization in forensic applications, such as the detection of latent fingerprints. We used chitosan crosslinked with sodium tripolyphosphate and conjugated with L-lysine to enhance the visualization of latent fingerprints, due to its ability to interact with fingerprint sweat residues. These conjugates were prepared using different (w/w) amounts of chitosan and tripolyphosphate (6/1; 4/1; 1/1; 1/4; and 1/6), and those with the most promising results (i.e., 6/1 formulation) were investigated in detail. Fourier transform infrared (FT-IR) spectroscopy confirmed interactions between components of the systems. Optical microscopy and scanning electron microscope (SEM) analysis showed that prepared powder formulations were uniform in size and confirmed that chitosan/tripolyphosphate/lysine conjugates bind easily to the sweat and lipid residues present in the latent fingerprints. The testing of prepared conjugates demonstrated the potential of these systems as bio-based powder substitution for commercially available powders.
T2  - Journal of Forensic Sciences
T1  - A novel chitosan/tripolyphosphate/L-lysine conjugates for latent fingerprints detection and enhancement
EP  - 160
IS  - 1
SP  - 149
VL  - 66
DO  - 10.1111/1556-4029.14569
ER  - 

@article{
author = "Vuckovic, Nemanja and Glodovic, Nikola and Radovanović, Željko and Janaćković, Đorđe and Milasinovic, Nikola",
year = "2021",
abstract = "Most chemical and physical methods employed in visualizing latent marks have shown detrimental effects on human health and, therefore, some research groups have directed their attention to the utilization of various (bio)polymers with the aforementioned purpose. Although chitosan is widely used in medicine, pharmacy, food, and drug delivery systems, there are very few studies that address this biopolymer utilization in forensic applications, such as the detection of latent fingerprints. We used chitosan crosslinked with sodium tripolyphosphate and conjugated with L-lysine to enhance the visualization of latent fingerprints, due to its ability to interact with fingerprint sweat residues. These conjugates were prepared using different (w/w) amounts of chitosan and tripolyphosphate (6/1; 4/1; 1/1; 1/4; and 1/6), and those with the most promising results (i.e., 6/1 formulation) were investigated in detail. Fourier transform infrared (FT-IR) spectroscopy confirmed interactions between components of the systems. Optical microscopy and scanning electron microscope (SEM) analysis showed that prepared powder formulations were uniform in size and confirmed that chitosan/tripolyphosphate/lysine conjugates bind easily to the sweat and lipid residues present in the latent fingerprints. The testing of prepared conjugates demonstrated the potential of these systems as bio-based powder substitution for commercially available powders.",
journal = "Journal of Forensic Sciences",
title = "A novel chitosan/tripolyphosphate/L-lysine conjugates for latent fingerprints detection and enhancement",
pages = "160-149",
number = "1",
volume = "66",
doi = "10.1111/1556-4029.14569"
}

Vuckovic, N., Glodovic, N., Radovanović, Ž., Janaćković, Đ.,& Milasinovic, N.. (2021). A novel chitosan/tripolyphosphate/L-lysine conjugates for latent fingerprints detection and enhancement. in Journal of Forensic Sciences, 66(1), 149-160.
https://doi.org/10.1111/1556-4029.14569

Vuckovic N, Glodovic N, Radovanović Ž, Janaćković Đ, Milasinovic N. A novel chitosan/tripolyphosphate/L-lysine conjugates for latent fingerprints detection and enhancement. in Journal of Forensic Sciences. 2021;66(1):149-160.
doi:10.1111/1556-4029.14569 .

Vuckovic, Nemanja, Glodovic, Nikola, Radovanović, Željko, Janaćković, Đorđe, Milasinovic, Nikola, "A novel chitosan/tripolyphosphate/L-lysine conjugates for latent fingerprints detection and enhancement" in Journal of Forensic Sciences, 66, no. 1 (2021):149-160,
https://doi.org/10.1111/1556-4029.14569 . .

TY  - THES
AU  - Marković, Maja
PY  - 2020
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=8124
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:23643/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=37501961
UR  - https://nardus.mpn.gov.rs/handle/123456789/18303
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5965
AB  - Hidrogelovi na bazi poli(metakrilne kiseline) - PMAA imaju veliku primenu u sistemimaza ciljano otpuštanje aktivnih supstanci zato što su pH osetljivi, netoksični i biokompatibilni.Ipak zbog izrazite hidrofilnosti ovi hidrogelovi se mogu koristiti samo za kontrolisano otpuštanjehidrofilnih supstanci. U ovoj disertaciji predstavljena je modifikacija PMAA hidrogelovaamfifilnim supstancama: proteinom - kazeinom i fosfolipidnim nanočesticama - lipozomima,kako bi se dobio nosač slabo vodorastvorne aktivne supstance (SVAS). Opisana je sintezanosača, njihova karakterizacija primenom infracrvene spektroskopije sa Furijeovomtransformacijom (FTIR) i skenirajućom elektronskom mikroskopijom (SEM) i karakterizacijalipozoma primenom dinamičkog rasipanja svetlosti (DLS) i metodom elektroforetskog rasipanjasvetlosti (ELS). FTIR spektri su pokazali da su hidrofobne interakcije uspostavljene izmeđuSVAS model leka – kofeina i kazeina omogućile inkapsulaciju kofeina i njegovo kontrolisanooslobađanje, dok su prisutni lipozomi omogućili bolju kontrolu brzine oslobađanja kofeina.Ispitan je i uticaj parametara sinteze (stepen neutralizacije metakrilne kiseline, koncentracijaumreživača i kofeina, prisustvo itakonske kiseline i lipozoma, udeo i oblik lipozomneformulacije) na morfološke karakteristike nosača, njihovo bubrenje i kinetiku oslobađanjakofeina u dve sredine različitih pH vrednosti koje su simulirale uslove prisutne ugastrointestinalnom traktu čoveka. Praćenje oslobađanja kofeina iz nosača u ovim sredinamaizvršeno je primenom ultraljubičaste spektroskopije (UV). Izvedena je detaljna analiza dobijeniheksperimentalnih podataka otpuštanja kofeina u in vitro uslovima, primenom nekolikomatematičkih modela. Najbolja kinetika otpuštanja kofeina postignuta je iz nosača sa potpunoneutralizovanom metakrilnom kiselinom, 1,6mol% umreživača i inkorporiranim lipozomima ukojima je inkapsuliran kofein. Ovaj nosač je odabran za dalje analize - ispitano je njegovoponašanje u uslovima koji su simulirali put nosača kroz gastrointestinalni trakt čoveka. Rezultatisu pokazali da kofein može da se inkapsulira u izabrani nosač u koncentracijama koje su više odnjegove maksimalne rastvorljivosti i da se postigne njihovo ciljano otpuštanje. Inkapsulacijakofeina u koncentracijama ovih vrednosti, postignuta je dodatkom nikotin-amida koji sakofeinom gradi stabilni kompleks. Dobijeni rezultati pokazuju da sintetisani nosači imaju velikipotencijal za ciljanu dostavu slabo vodorastvotnih supstanci.
AB  - Hydrogels based on poly(methacrylic acid) (PMAA) have been extensively used insystems for targeted delivery of active substances because they are pH-sensitive, non-toxic andbiocompatible. However, being highly hydrophilic, these hydrogels are able to deliver onlyhydrophilic active substances. In this dissertation, modification of PMAA hydrogels withamphiphilic substances: protein - casein and phospholipidic nanoparticles - liposomes in order todesign a hydrophilic carrier for a poorly water-soluble substance (PWSS) are presented. Thesynthesis of the carriers and their characterization using the Fourier Transform InfraredSpectroscopy (FTIR) and the Scanning Electron Microscopy (SEM) and the characterization ofthe liposomes using the Dynamic Light Scattering (DLS) and the method of the ElectrophoreticLight Scattering (ELS) are described. The FTIR spectra showed that hydrophobic interactionswere established between PWSS model drug - caffeine and casein and enabled caffeineencapsulation in the carrier and its controlled release, while the presence of the liposomesenabled better control of caffeine release rate. The morphological characteristics of the carriers,their swelling behavior and the caffeine release kinetics were investigated depending on thechanges in the synthesis parameters (neutralization degree of methacrylic acid, crosslinker andcaffeine concentrations, introduction of itaconic acid and liposomes, volume ratio and form ofliposomal formulation) in two media with different pH values that simulated the environment inhuman gastrointestinal tract. Ultraviolet (UV) Spectroscopy was used to monitor caffeine releasefrom these carriers. Data from caffeine in vitro release experiments were analyzed in detail usingseveral mathematical models. The best kinetic of caffeine release was achieved form the carrierin which methacrylic acid was complitely neutralized and which had 1,6mol% of crosslinker andincorporated liposomes with encapsulated caffeine. The behavior of this carrier was furtherinvestigated in the environment which simulated the conditions in the human gastrointestinaltract. Results showed that this carrier could be used for the encapsulation of caffeine inconcentrations which were higher than its solubility and for targeted delivery of theseconcentrations. Encapsulation of caffeine in these values of concentration was achieved byadding nicotinamide, which forms stable complex with caffeine. Presented results showed thatsynthesized carriers have great potential for targeted delivery of poorly water-soluble substances.
PB  - Универзитет у Београду, Технолошко-металуршки факултет
T2  - Универзитет у Београду
T1  - Kinetika oslobađanja slabo vodorastvornih aktivnih supstanci iz nosača na bazi poli(metakrilne kiseline), kazeina i lipozoma
UR  - https://hdl.handle.net/21.15107/rcub_nardus_18303
ER  - 

@phdthesis{
author = "Marković, Maja",
year = "2020",
abstract = "Hidrogelovi na bazi poli(metakrilne kiseline) - PMAA imaju veliku primenu u sistemimaza ciljano otpuštanje aktivnih supstanci zato što su pH osetljivi, netoksični i biokompatibilni.Ipak zbog izrazite hidrofilnosti ovi hidrogelovi se mogu koristiti samo za kontrolisano otpuštanjehidrofilnih supstanci. U ovoj disertaciji predstavljena je modifikacija PMAA hidrogelovaamfifilnim supstancama: proteinom - kazeinom i fosfolipidnim nanočesticama - lipozomima,kako bi se dobio nosač slabo vodorastvorne aktivne supstance (SVAS). Opisana je sintezanosača, njihova karakterizacija primenom infracrvene spektroskopije sa Furijeovomtransformacijom (FTIR) i skenirajućom elektronskom mikroskopijom (SEM) i karakterizacijalipozoma primenom dinamičkog rasipanja svetlosti (DLS) i metodom elektroforetskog rasipanjasvetlosti (ELS). FTIR spektri su pokazali da su hidrofobne interakcije uspostavljene izmeđuSVAS model leka – kofeina i kazeina omogućile inkapsulaciju kofeina i njegovo kontrolisanooslobađanje, dok su prisutni lipozomi omogućili bolju kontrolu brzine oslobađanja kofeina.Ispitan je i uticaj parametara sinteze (stepen neutralizacije metakrilne kiseline, koncentracijaumreživača i kofeina, prisustvo itakonske kiseline i lipozoma, udeo i oblik lipozomneformulacije) na morfološke karakteristike nosača, njihovo bubrenje i kinetiku oslobađanjakofeina u dve sredine različitih pH vrednosti koje su simulirale uslove prisutne ugastrointestinalnom traktu čoveka. Praćenje oslobađanja kofeina iz nosača u ovim sredinamaizvršeno je primenom ultraljubičaste spektroskopije (UV). Izvedena je detaljna analiza dobijeniheksperimentalnih podataka otpuštanja kofeina u in vitro uslovima, primenom nekolikomatematičkih modela. Najbolja kinetika otpuštanja kofeina postignuta je iz nosača sa potpunoneutralizovanom metakrilnom kiselinom, 1,6mol% umreživača i inkorporiranim lipozomima ukojima je inkapsuliran kofein. Ovaj nosač je odabran za dalje analize - ispitano je njegovoponašanje u uslovima koji su simulirali put nosača kroz gastrointestinalni trakt čoveka. Rezultatisu pokazali da kofein može da se inkapsulira u izabrani nosač u koncentracijama koje su više odnjegove maksimalne rastvorljivosti i da se postigne njihovo ciljano otpuštanje. Inkapsulacijakofeina u koncentracijama ovih vrednosti, postignuta je dodatkom nikotin-amida koji sakofeinom gradi stabilni kompleks. Dobijeni rezultati pokazuju da sintetisani nosači imaju velikipotencijal za ciljanu dostavu slabo vodorastvotnih supstanci., Hydrogels based on poly(methacrylic acid) (PMAA) have been extensively used insystems for targeted delivery of active substances because they are pH-sensitive, non-toxic andbiocompatible. However, being highly hydrophilic, these hydrogels are able to deliver onlyhydrophilic active substances. In this dissertation, modification of PMAA hydrogels withamphiphilic substances: protein - casein and phospholipidic nanoparticles - liposomes in order todesign a hydrophilic carrier for a poorly water-soluble substance (PWSS) are presented. Thesynthesis of the carriers and their characterization using the Fourier Transform InfraredSpectroscopy (FTIR) and the Scanning Electron Microscopy (SEM) and the characterization ofthe liposomes using the Dynamic Light Scattering (DLS) and the method of the ElectrophoreticLight Scattering (ELS) are described. The FTIR spectra showed that hydrophobic interactionswere established between PWSS model drug - caffeine and casein and enabled caffeineencapsulation in the carrier and its controlled release, while the presence of the liposomesenabled better control of caffeine release rate. The morphological characteristics of the carriers,their swelling behavior and the caffeine release kinetics were investigated depending on thechanges in the synthesis parameters (neutralization degree of methacrylic acid, crosslinker andcaffeine concentrations, introduction of itaconic acid and liposomes, volume ratio and form ofliposomal formulation) in two media with different pH values that simulated the environment inhuman gastrointestinal tract. Ultraviolet (UV) Spectroscopy was used to monitor caffeine releasefrom these carriers. Data from caffeine in vitro release experiments were analyzed in detail usingseveral mathematical models. The best kinetic of caffeine release was achieved form the carrierin which methacrylic acid was complitely neutralized and which had 1,6mol% of crosslinker andincorporated liposomes with encapsulated caffeine. The behavior of this carrier was furtherinvestigated in the environment which simulated the conditions in the human gastrointestinaltract. Results showed that this carrier could be used for the encapsulation of caffeine inconcentrations which were higher than its solubility and for targeted delivery of theseconcentrations. Encapsulation of caffeine in these values of concentration was achieved byadding nicotinamide, which forms stable complex with caffeine. Presented results showed thatsynthesized carriers have great potential for targeted delivery of poorly water-soluble substances.",
publisher = "Универзитет у Београду, Технолошко-металуршки факултет",
journal = "Универзитет у Београду",
title = "Kinetika oslobađanja slabo vodorastvornih aktivnih supstanci iz nosača na bazi poli(metakrilne kiseline), kazeina i lipozoma",
url = "https://hdl.handle.net/21.15107/rcub_nardus_18303"
}

Marković, M.. (2020). Kinetika oslobađanja slabo vodorastvornih aktivnih supstanci iz nosača na bazi poli(metakrilne kiseline), kazeina i lipozoma. in Универзитет у Београду
Универзитет у Београду, Технолошко-металуршки факултет..
https://hdl.handle.net/21.15107/rcub_nardus_18303

Marković M. Kinetika oslobađanja slabo vodorastvornih aktivnih supstanci iz nosača na bazi poli(metakrilne kiseline), kazeina i lipozoma. in Универзитет у Београду. 2020;.
https://hdl.handle.net/21.15107/rcub_nardus_18303 .

Marković, Maja, "Kinetika oslobađanja slabo vodorastvornih aktivnih supstanci iz nosača na bazi poli(metakrilne kiseline), kazeina i lipozoma" in Универзитет у Београду (2020),
https://hdl.handle.net/21.15107/rcub_nardus_18303 .

TY  - JOUR
AU  - Obradović, Nataša
AU  - Pajić-Lijaković, Ivana
AU  - Krunić, Tanja
AU  - Belović, Miona
AU  - Rakin, Marica
AU  - Bugarski, Branko
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4463
AB  - The suitability of natural hydrogel carriers with probiotic starter culture as whey beverages supplements was examined by assessing their rheological and structural changes during the fermentation and gastrointestinal conditions. Effect of encapsulated cells on the carrier structure is of great importance for the selection of proper material for the preparation of functional supplements. The structural changes of the chitosan-coated alginate/whey carriers were considered based on (1) cell viability and the carrier average volume vs. time (2) the storage and loss modulus vs. time obtained under low oscillator strain conditions, (3) FTIR analysis and (4) SEM cross-sectional observation of the hydrogel carriers. The presence of chitosan coating and fermentation conditions increased cell viability up to 9.01 +/- 0.18 (log CFU/g). According to our results, the encapsulated cells induce weakening of carriers under the gastric conditions but improve their mechanical stability under the intestinal condition. The mechanical behaviour of carriers was also considered in order to formulate the rheological constitutive model equation for describing the irreversible structural changes under the gastric and intestinal conditions. The cell leakage under the gastric condition after the 2 h was less than 5%. Carriers are rapidly degraded under the intestinal condition which ensures the release of cells and provides their beneficial effects on the host health. Our results indicate that this type of coated carrier is suitable to be used for encapsulation of probiotic starter culture in the production of fermented whey-based products.
PB  - Springer, New York
T2  - Food Biophysics
T1  - Effect of Encapsulated Probiotic Starter Culture on Rheological and Structural Properties of Natural Hydrogel Carriers Affected by Fermentation and Gastrointestinal Conditions
EP  - 31
IS  - 1
SP  - 18
VL  - 15
DO  - 10.1007/s11483-019-09598-8
ER  - 

@article{
author = "Obradović, Nataša and Pajić-Lijaković, Ivana and Krunić, Tanja and Belović, Miona and Rakin, Marica and Bugarski, Branko",
year = "2020",
abstract = "The suitability of natural hydrogel carriers with probiotic starter culture as whey beverages supplements was examined by assessing their rheological and structural changes during the fermentation and gastrointestinal conditions. Effect of encapsulated cells on the carrier structure is of great importance for the selection of proper material for the preparation of functional supplements. The structural changes of the chitosan-coated alginate/whey carriers were considered based on (1) cell viability and the carrier average volume vs. time (2) the storage and loss modulus vs. time obtained under low oscillator strain conditions, (3) FTIR analysis and (4) SEM cross-sectional observation of the hydrogel carriers. The presence of chitosan coating and fermentation conditions increased cell viability up to 9.01 +/- 0.18 (log CFU/g). According to our results, the encapsulated cells induce weakening of carriers under the gastric conditions but improve their mechanical stability under the intestinal condition. The mechanical behaviour of carriers was also considered in order to formulate the rheological constitutive model equation for describing the irreversible structural changes under the gastric and intestinal conditions. The cell leakage under the gastric condition after the 2 h was less than 5%. Carriers are rapidly degraded under the intestinal condition which ensures the release of cells and provides their beneficial effects on the host health. Our results indicate that this type of coated carrier is suitable to be used for encapsulation of probiotic starter culture in the production of fermented whey-based products.",
publisher = "Springer, New York",
journal = "Food Biophysics",
title = "Effect of Encapsulated Probiotic Starter Culture on Rheological and Structural Properties of Natural Hydrogel Carriers Affected by Fermentation and Gastrointestinal Conditions",
pages = "31-18",
number = "1",
volume = "15",
doi = "10.1007/s11483-019-09598-8"
}

Obradović, N., Pajić-Lijaković, I., Krunić, T., Belović, M., Rakin, M.,& Bugarski, B.. (2020). Effect of Encapsulated Probiotic Starter Culture on Rheological and Structural Properties of Natural Hydrogel Carriers Affected by Fermentation and Gastrointestinal Conditions. in Food Biophysics
Springer, New York., 15(1), 18-31.
https://doi.org/10.1007/s11483-019-09598-8

Obradović N, Pajić-Lijaković I, Krunić T, Belović M, Rakin M, Bugarski B. Effect of Encapsulated Probiotic Starter Culture on Rheological and Structural Properties of Natural Hydrogel Carriers Affected by Fermentation and Gastrointestinal Conditions. in Food Biophysics. 2020;15(1):18-31.
doi:10.1007/s11483-019-09598-8 .

Obradović, Nataša, Pajić-Lijaković, Ivana, Krunić, Tanja, Belović, Miona, Rakin, Marica, Bugarski, Branko, "Effect of Encapsulated Probiotic Starter Culture on Rheological and Structural Properties of Natural Hydrogel Carriers Affected by Fermentation and Gastrointestinal Conditions" in Food Biophysics, 15, no. 1 (2020):18-31,
https://doi.org/10.1007/s11483-019-09598-8 . .

TY  - JOUR
AU  - Marković, Maja
AU  - Panić, Vesna
AU  - Šešlija, Sanja
AU  - Spasojević, Pavle
AU  - Ugrinović, Vukašin
AU  - Bošković-Vragolović, Nevenka
AU  - Pjanović, Rada
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4382
AB  - pH sensitive, nontoxic, and biocompatible poly(methacrylic) acid (PMAA) based soft networks have been extensively used in the design of systems for targeted drug delivery. Still, their highly hydrophilic nature limits their potential to be used as a carrier of poorly water-soluble substances. With the aim to overcome this limitation, the present study details a new approach for modification of PMAA based carriers using two amphiphilic components: casein and liposomes. The FTIR analysis revealed structural features of each component as well as the synergetic effect that originated from the formation of specific interactions. Namely, hydrophobic interactions between the poorly water-soluble model drug (caffeine) and casein enabled caffeine encapsulation and controlled release, while addition of liposomes ensured better control of the release rate. The morphological properties of the carriers, swelling behavior, and release kinetics of caffeine were investigated depending on the variable synthesis parameters (neutralization degree of methacrylic acid, concentration of caffeine, presence/absence of liposomes) in two different media simulating the pH environment of human intestines and stomach. The data obtained from in vitro caffeine release were correlated and analyzed in detail using several mathematical models, indicating significant potential of investigated carriers for targeted delivery and controlled release of poorly water-soluble substances.
PB  - Wiley, Hoboken
T2  - Polymer Engineering and Science
T1  - Modification of hydrophilic polymer network to design a carrier for a poorly water-soluble substance
EP  - 2510
IS  - 10
SP  - 2496
VL  - 60
DO  - 10.1002/pen.25487
ER  - 

@article{
author = "Marković, Maja and Panić, Vesna and Šešlija, Sanja and Spasojević, Pavle and Ugrinović, Vukašin and Bošković-Vragolović, Nevenka and Pjanović, Rada",
year = "2020",
abstract = "pH sensitive, nontoxic, and biocompatible poly(methacrylic) acid (PMAA) based soft networks have been extensively used in the design of systems for targeted drug delivery. Still, their highly hydrophilic nature limits their potential to be used as a carrier of poorly water-soluble substances. With the aim to overcome this limitation, the present study details a new approach for modification of PMAA based carriers using two amphiphilic components: casein and liposomes. The FTIR analysis revealed structural features of each component as well as the synergetic effect that originated from the formation of specific interactions. Namely, hydrophobic interactions between the poorly water-soluble model drug (caffeine) and casein enabled caffeine encapsulation and controlled release, while addition of liposomes ensured better control of the release rate. The morphological properties of the carriers, swelling behavior, and release kinetics of caffeine were investigated depending on the variable synthesis parameters (neutralization degree of methacrylic acid, concentration of caffeine, presence/absence of liposomes) in two different media simulating the pH environment of human intestines and stomach. The data obtained from in vitro caffeine release were correlated and analyzed in detail using several mathematical models, indicating significant potential of investigated carriers for targeted delivery and controlled release of poorly water-soluble substances.",
publisher = "Wiley, Hoboken",
journal = "Polymer Engineering and Science",
title = "Modification of hydrophilic polymer network to design a carrier for a poorly water-soluble substance",
pages = "2510-2496",
number = "10",
volume = "60",
doi = "10.1002/pen.25487"
}

Marković, M., Panić, V., Šešlija, S., Spasojević, P., Ugrinović, V., Bošković-Vragolović, N.,& Pjanović, R.. (2020). Modification of hydrophilic polymer network to design a carrier for a poorly water-soluble substance. in Polymer Engineering and Science
Wiley, Hoboken., 60(10), 2496-2510.
https://doi.org/10.1002/pen.25487

Marković M, Panić V, Šešlija S, Spasojević P, Ugrinović V, Bošković-Vragolović N, Pjanović R. Modification of hydrophilic polymer network to design a carrier for a poorly water-soluble substance. in Polymer Engineering and Science. 2020;60(10):2496-2510.
doi:10.1002/pen.25487 .

Marković, Maja, Panić, Vesna, Šešlija, Sanja, Spasojević, Pavle, Ugrinović, Vukašin, Bošković-Vragolović, Nevenka, Pjanović, Rada, "Modification of hydrophilic polymer network to design a carrier for a poorly water-soluble substance" in Polymer Engineering and Science, 60, no. 10 (2020):2496-2510,
https://doi.org/10.1002/pen.25487 . .

TY  - JOUR
AU  - Marković, Maja
AU  - Panić, Vesna
AU  - Šešlija, Sanja
AU  - Milivojević, Ana
AU  - Spasojević, Pavle
AU  - Bošković-Vragolović, Nevenka
AU  - Pjanović, Rada
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4541
AB  - Carriers for targeted delivery and controlled release of poorly water-soluble active substances (PWSAS) are facing three challenges: (a) the encapsulation issues, (b) limitations of PWSAS water solubility, and
PB  - Wiley, Hoboken
T2  - Polymer Engineering and Science
T1  - Novel strategy for encapsulation and targeted delivery of poorly water-soluble active substances
EP  - 2022
IS  - 8
SP  - 2008
VL  - 60
DO  - 10.1002/pen.25448
ER  - 

@article{
author = "Marković, Maja and Panić, Vesna and Šešlija, Sanja and Milivojević, Ana and Spasojević, Pavle and Bošković-Vragolović, Nevenka and Pjanović, Rada",
year = "2020",
abstract = "Carriers for targeted delivery and controlled release of poorly water-soluble active substances (PWSAS) are facing three challenges: (a) the encapsulation issues, (b) limitations of PWSAS water solubility, and",
publisher = "Wiley, Hoboken",
journal = "Polymer Engineering and Science",
title = "Novel strategy for encapsulation and targeted delivery of poorly water-soluble active substances",
pages = "2022-2008",
number = "8",
volume = "60",
doi = "10.1002/pen.25448"
}

Marković, M., Panić, V., Šešlija, S., Milivojević, A., Spasojević, P., Bošković-Vragolović, N.,& Pjanović, R.. (2020). Novel strategy for encapsulation and targeted delivery of poorly water-soluble active substances. in Polymer Engineering and Science
Wiley, Hoboken., 60(8), 2008-2022.
https://doi.org/10.1002/pen.25448

Marković M, Panić V, Šešlija S, Milivojević A, Spasojević P, Bošković-Vragolović N, Pjanović R. Novel strategy for encapsulation and targeted delivery of poorly water-soluble active substances. in Polymer Engineering and Science. 2020;60(8):2008-2022.
doi:10.1002/pen.25448 .

Marković, Maja, Panić, Vesna, Šešlija, Sanja, Milivojević, Ana, Spasojević, Pavle, Bošković-Vragolović, Nevenka, Pjanović, Rada, "Novel strategy for encapsulation and targeted delivery of poorly water-soluble active substances" in Polymer Engineering and Science, 60, no. 8 (2020):2008-2022,
https://doi.org/10.1002/pen.25448 . .

TY  - JOUR
AU  - Šekuljica, Nataša
AU  - Jovanović, Jelena
AU  - Jakovetić Tanasković, Sonja
AU  - Ognjanović, Nevena
AU  - Gazikalović, Ivana
AU  - Knežević-Jugović, Zorica
AU  - Mijin, Dušan
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4457
AB  - Horseradish peroxidase (HRP) is a highly specific enzyme with great potential for use in the decolorization of synthetic dyes. A comprehensive study of HRP immobilization using various techniques such as adsorption and covalent immobilization on the novel carrier Purolite (R) A109 with a special focus on enzymatic decolorization and toxicity of artificially colored wastewater. The immobilized preparations with an activity of 156.21 +/- 1.41 U g(-1) and 85.71 +/- 1.62 U g(-1) after the HRP adsorption and covalent immobilization, respectively, were obtained. Stability and reusability of the immobilized preparations were also evaluated. A noteworthy decolorization level (similar to 90%) with immobilized HRP was achieved. Phytotoxicity testing using Mung bean seeds and acute toxicity assay with Artemia salina has confirmed the applicability of the obtained immobilized preparation in industrial wastewater plants for the treatment of colored wastewater.
PB  - Wiley, Hoboken
T2  - Biotechnology Progress
T1  - Immobilization of horseradish peroxidase onto Purolite (R) A109 and its anthraquinone dye biodegradation and detoxification potential
IS  - 4
VL  - 36
DO  - 10.1002/btpr.2991
ER  - 

@article{
author = "Šekuljica, Nataša and Jovanović, Jelena and Jakovetić Tanasković, Sonja and Ognjanović, Nevena and Gazikalović, Ivana and Knežević-Jugović, Zorica and Mijin, Dušan",
year = "2020",
abstract = "Horseradish peroxidase (HRP) is a highly specific enzyme with great potential for use in the decolorization of synthetic dyes. A comprehensive study of HRP immobilization using various techniques such as adsorption and covalent immobilization on the novel carrier Purolite (R) A109 with a special focus on enzymatic decolorization and toxicity of artificially colored wastewater. The immobilized preparations with an activity of 156.21 +/- 1.41 U g(-1) and 85.71 +/- 1.62 U g(-1) after the HRP adsorption and covalent immobilization, respectively, were obtained. Stability and reusability of the immobilized preparations were also evaluated. A noteworthy decolorization level (similar to 90%) with immobilized HRP was achieved. Phytotoxicity testing using Mung bean seeds and acute toxicity assay with Artemia salina has confirmed the applicability of the obtained immobilized preparation in industrial wastewater plants for the treatment of colored wastewater.",
publisher = "Wiley, Hoboken",
journal = "Biotechnology Progress",
title = "Immobilization of horseradish peroxidase onto Purolite (R) A109 and its anthraquinone dye biodegradation and detoxification potential",
number = "4",
volume = "36",
doi = "10.1002/btpr.2991"
}

Šekuljica, N., Jovanović, J., Jakovetić Tanasković, S., Ognjanović, N., Gazikalović, I., Knežević-Jugović, Z.,& Mijin, D.. (2020). Immobilization of horseradish peroxidase onto Purolite (R) A109 and its anthraquinone dye biodegradation and detoxification potential. in Biotechnology Progress
Wiley, Hoboken., 36(4).
https://doi.org/10.1002/btpr.2991

Šekuljica N, Jovanović J, Jakovetić Tanasković S, Ognjanović N, Gazikalović I, Knežević-Jugović Z, Mijin D. Immobilization of horseradish peroxidase onto Purolite (R) A109 and its anthraquinone dye biodegradation and detoxification potential. in Biotechnology Progress. 2020;36(4).
doi:10.1002/btpr.2991 .

Šekuljica, Nataša, Jovanović, Jelena, Jakovetić Tanasković, Sonja, Ognjanović, Nevena, Gazikalović, Ivana, Knežević-Jugović, Zorica, Mijin, Dušan, "Immobilization of horseradish peroxidase onto Purolite (R) A109 and its anthraquinone dye biodegradation and detoxification potential" in Biotechnology Progress, 36, no. 4 (2020),
https://doi.org/10.1002/btpr.2991 . .

TY  - JOUR
AU  - Bebić, Jelena
AU  - Banjanac, Katarina
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Simović, Milica
AU  - Marinković, Aleksandar
AU  - Bezbradica, Dejan
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4384
AB  - This work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oiyzae (Novozym 51003 (R) laccase) on amino modified fumed nano-silica (AFNS) and the possible use in bioremediation. Hereby, for the first time, factors affecting the immobilization of Novozym 51003 (R) laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase immobilization. The highest specific activity (13.1 IU.mg(-1) proteins) was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield, reaching 68.3% after the equilibrium time, at optimum pH 5.0, was obtained. Laccase immobilization occurs by adsorption as monolayer enzyme binding in 40 min, following pseudo-first-order kinetics. The possible use of obtained immobilized preparation was investigated in degradation of pesticide lindane. Within 24 h, lindane concentration was reduced to 56.8% of initial concentration and after seven repeated reuses it retained 70% of the original activity.
PB  - Chemical Industry Press Co Ltd, Beijing
T2  - Chinese Journal of Chemical Engineering
T1  - Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation
EP  - 1144
IS  - 4
SP  - 1136
VL  - 28
DO  - 10.1016/j.cjche.2019.12.025
ER  - 

@article{
author = "Bebić, Jelena and Banjanac, Katarina and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Marinković, Aleksandar and Bezbradica, Dejan",
year = "2020",
abstract = "This work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oiyzae (Novozym 51003 (R) laccase) on amino modified fumed nano-silica (AFNS) and the possible use in bioremediation. Hereby, for the first time, factors affecting the immobilization of Novozym 51003 (R) laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase immobilization. The highest specific activity (13.1 IU.mg(-1) proteins) was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield, reaching 68.3% after the equilibrium time, at optimum pH 5.0, was obtained. Laccase immobilization occurs by adsorption as monolayer enzyme binding in 40 min, following pseudo-first-order kinetics. The possible use of obtained immobilized preparation was investigated in degradation of pesticide lindane. Within 24 h, lindane concentration was reduced to 56.8% of initial concentration and after seven repeated reuses it retained 70% of the original activity.",
publisher = "Chemical Industry Press Co Ltd, Beijing",
journal = "Chinese Journal of Chemical Engineering",
title = "Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation",
pages = "1144-1136",
number = "4",
volume = "28",
doi = "10.1016/j.cjche.2019.12.025"
}

Bebić, J., Banjanac, K., Ćorović, M., Milivojević, A., Simović, M., Marinković, A.,& Bezbradica, D.. (2020). Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation. in Chinese Journal of Chemical Engineering
Chemical Industry Press Co Ltd, Beijing., 28(4), 1136-1144.
https://doi.org/10.1016/j.cjche.2019.12.025

Bebić J, Banjanac K, Ćorović M, Milivojević A, Simović M, Marinković A, Bezbradica D. Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation. in Chinese Journal of Chemical Engineering. 2020;28(4):1136-1144.
doi:10.1016/j.cjche.2019.12.025 .

Bebić, Jelena, Banjanac, Katarina, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Marinković, Aleksandar, Bezbradica, Dejan, "Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation" in Chinese Journal of Chemical Engineering, 28, no. 4 (2020):1136-1144,
https://doi.org/10.1016/j.cjche.2019.12.025 . .

TY  - JOUR
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Pjanović, Rada
AU  - Bezbradica, Dejan
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4494
AB  - Lipase-catalyzed transesterification of vitamin C by natural triglycerides is promising approach for cost-efficient synthesis of liposoluble food and cosmetic antioxidants. Nevertheless, application of these alternative acyl donors is insufficiently explored, despite of their low price, wide abundance and possibility for obtaining versatile products. Within current study, fatty acid ascorbyl esters were synthesized from vitamin C and natural triglycerides (lard, sunflower, coconut and linseed oil) in a process catalyzed by immobilized lipase Novozym (R) 435 and their controlled release was examined. High conversions were achieved at optimized conditions, even at high substrate concentrations, thus high concentrations of acsorbyl-ester were synthesized using these natural lipids. All ester mixtures exhibited very high capacity for scavenging of DPPH radicals, among which linseed oil derived esters, with ascorbyl linolenate as prevailing compound, were the most efficient (IC50 0.663 mu M), while coconut oil derived ester mixture (IC50 0.739 mu M), composed predominantly of molecules with medium side chains, was second best. Coconut oil derived esters were successfully incorporated in typical cosmetic formulations for controlled release of bioactive compounds - O/W emulsion and gel-emulsion. Franz cell diffusion study demonstrated that esters release from mixture and two carrier systems was formulation dependent and revealed decrease of effective diffusivities with ester side acyl chain length increase. According to calculated effective diffusivity coefficients, faster trans-membrane delivery of the same molecule was achieved from gel-emulsion comparing to O/W emulsion. Applied approach enabled highly efficient cost-saving production of fatty acid ascorbyl esters, which could find direct application in various lipophilic products.
PB  - Elsevier B.V.
T2  - Sustainable Chemistry and Pharmacy
T1  - Enzymatically derived oil-based L-ascorbyl esters: Synthesis, antioxidant properties and controlled release from cosmetic formulations
SP  - 100231
VL  - 15
DO  - 10.1016/j.scp.2020.100231
ER  - 

@article{
author = "Ćorović, Marija and Milivojević, Ana and Simović, Milica and Banjanac, Katarina and Pjanović, Rada and Bezbradica, Dejan",
year = "2020",
abstract = "Lipase-catalyzed transesterification of vitamin C by natural triglycerides is promising approach for cost-efficient synthesis of liposoluble food and cosmetic antioxidants. Nevertheless, application of these alternative acyl donors is insufficiently explored, despite of their low price, wide abundance and possibility for obtaining versatile products. Within current study, fatty acid ascorbyl esters were synthesized from vitamin C and natural triglycerides (lard, sunflower, coconut and linseed oil) in a process catalyzed by immobilized lipase Novozym (R) 435 and their controlled release was examined. High conversions were achieved at optimized conditions, even at high substrate concentrations, thus high concentrations of acsorbyl-ester were synthesized using these natural lipids. All ester mixtures exhibited very high capacity for scavenging of DPPH radicals, among which linseed oil derived esters, with ascorbyl linolenate as prevailing compound, were the most efficient (IC50 0.663 mu M), while coconut oil derived ester mixture (IC50 0.739 mu M), composed predominantly of molecules with medium side chains, was second best. Coconut oil derived esters were successfully incorporated in typical cosmetic formulations for controlled release of bioactive compounds - O/W emulsion and gel-emulsion. Franz cell diffusion study demonstrated that esters release from mixture and two carrier systems was formulation dependent and revealed decrease of effective diffusivities with ester side acyl chain length increase. According to calculated effective diffusivity coefficients, faster trans-membrane delivery of the same molecule was achieved from gel-emulsion comparing to O/W emulsion. Applied approach enabled highly efficient cost-saving production of fatty acid ascorbyl esters, which could find direct application in various lipophilic products.",
publisher = "Elsevier B.V.",
journal = "Sustainable Chemistry and Pharmacy",
title = "Enzymatically derived oil-based L-ascorbyl esters: Synthesis, antioxidant properties and controlled release from cosmetic formulations",
pages = "100231",
volume = "15",
doi = "10.1016/j.scp.2020.100231"
}

Ćorović, M., Milivojević, A., Simović, M., Banjanac, K., Pjanović, R.,& Bezbradica, D.. (2020). Enzymatically derived oil-based L-ascorbyl esters: Synthesis, antioxidant properties and controlled release from cosmetic formulations. in Sustainable Chemistry and Pharmacy
Elsevier B.V.., 15, 100231.
https://doi.org/10.1016/j.scp.2020.100231

Ćorović M, Milivojević A, Simović M, Banjanac K, Pjanović R, Bezbradica D. Enzymatically derived oil-based L-ascorbyl esters: Synthesis, antioxidant properties and controlled release from cosmetic formulations. in Sustainable Chemistry and Pharmacy. 2020;15:100231.
doi:10.1016/j.scp.2020.100231 .

Ćorović, Marija, Milivojević, Ana, Simović, Milica, Banjanac, Katarina, Pjanović, Rada, Bezbradica, Dejan, "Enzymatically derived oil-based L-ascorbyl esters: Synthesis, antioxidant properties and controlled release from cosmetic formulations" in Sustainable Chemistry and Pharmacy, 15 (2020):100231,
https://doi.org/10.1016/j.scp.2020.100231 . .

TY  - JOUR
AU  - Losada-Garcia, Noelia
AU  - Rodriguez-Oliva, Ivan
AU  - Simović, Milica
AU  - Bezbradica, Dejan
AU  - Palomo, Jose M.
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4486
AB  - Glycoderivatives are an important class of molecules with enormous relevance in numerous biological phenomena; therefore, they have a key role in the learning, understanding, and assessment of different diseases. Nanotechnology, and in particular the design of new nanomaterials, is one of the areas of greatest interest today. In this case, graphene nanomaterials represent very interesting platforms for studying glycosystems, glyconanomaterials that combine the biomolecular recognition and the characteristics of nanoscale objects in the development of early diagnosis systems, and efficient specific therapeutic modalities. In this mini-review, we discuss some results recently described in the literature on the conjugation of graphene materials and carbohydrates through the selective interaction of glycoenzymes in graphene to create new materials with biosensing applications, the development and application of sugar-graphene composites, and finally biosystems combining the properties of graphene with metallic nanoparticles and sugars for the creation of excellent glyconanomaterials as novel systems for the therapy or diagnosis of important diseases such as cancer or diabetes.
PB  - Amer Chemical Soc, Washington
T2  - ACS Omega
T1  - New Advances in Fabrication of Graphene Glyconanomaterials for Application in Therapy and Diagnosis
EP  - 4369
IS  - 9
SP  - 4362
VL  - 5
DO  - 10.1021/acsomega.9b04332
ER  - 

@article{
author = "Losada-Garcia, Noelia and Rodriguez-Oliva, Ivan and Simović, Milica and Bezbradica, Dejan and Palomo, Jose M.",
year = "2020",
abstract = "Glycoderivatives are an important class of molecules with enormous relevance in numerous biological phenomena; therefore, they have a key role in the learning, understanding, and assessment of different diseases. Nanotechnology, and in particular the design of new nanomaterials, is one of the areas of greatest interest today. In this case, graphene nanomaterials represent very interesting platforms for studying glycosystems, glyconanomaterials that combine the biomolecular recognition and the characteristics of nanoscale objects in the development of early diagnosis systems, and efficient specific therapeutic modalities. In this mini-review, we discuss some results recently described in the literature on the conjugation of graphene materials and carbohydrates through the selective interaction of glycoenzymes in graphene to create new materials with biosensing applications, the development and application of sugar-graphene composites, and finally biosystems combining the properties of graphene with metallic nanoparticles and sugars for the creation of excellent glyconanomaterials as novel systems for the therapy or diagnosis of important diseases such as cancer or diabetes.",
publisher = "Amer Chemical Soc, Washington",
journal = "ACS Omega",
title = "New Advances in Fabrication of Graphene Glyconanomaterials for Application in Therapy and Diagnosis",
pages = "4369-4362",
number = "9",
volume = "5",
doi = "10.1021/acsomega.9b04332"
}

Losada-Garcia, N., Rodriguez-Oliva, I., Simović, M., Bezbradica, D.,& Palomo, J. M.. (2020). New Advances in Fabrication of Graphene Glyconanomaterials for Application in Therapy and Diagnosis. in ACS Omega
Amer Chemical Soc, Washington., 5(9), 4362-4369.
https://doi.org/10.1021/acsomega.9b04332

Losada-Garcia N, Rodriguez-Oliva I, Simović M, Bezbradica D, Palomo JM. New Advances in Fabrication of Graphene Glyconanomaterials for Application in Therapy and Diagnosis. in ACS Omega. 2020;5(9):4362-4369.
doi:10.1021/acsomega.9b04332 .

Losada-Garcia, Noelia, Rodriguez-Oliva, Ivan, Simović, Milica, Bezbradica, Dejan, Palomo, Jose M., "New Advances in Fabrication of Graphene Glyconanomaterials for Application in Therapy and Diagnosis" in ACS Omega, 5, no. 9 (2020):4362-4369,
https://doi.org/10.1021/acsomega.9b04332 . .

TY  - JOUR
AU  - Radosavljević, Miloš
AU  - Lević, Steva
AU  - Belović, Miona
AU  - Pejin, Jelena
AU  - Đukić-Vuković, Aleksandra
AU  - Mojović, Ljiljana
AU  - Nedović, Viktor
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4344
AB  - Immobilization of Lactobacillus rhamnosus ATCC7469 in poly(vinyl alcohol)/calcium alginate (PVA/Ca-alginate) matrix using "freezing-thawing" technique for application in lactic acid (LA) fermentation was studied in this paper. PVA/Ca-alginate beads were made from sterile and non-sterile PVA and sodium alginate solutions. According to mechanical properties, the PVA/Ca-alginate beads expressed a strong elastic character. Obtained PVA/Ca-alginate beads were further applied in batch and repeated batch LA fermentations. Regarding cell viability, L. rhamnosus cells survived well rather sharp immobilization procedure and significant cell proliferation was observed in further fermentation studies achieving high cell viability (up to 10.7 log CFU g(-1)) in sterile beads. In batch LA fermentation, the immobilized biocatalyst was superior to free cell fermentation system (by 37.1%), while the highest LA yield and volumetric productivity of 97.6% and 0.8 g L-1 h(-1), respectively, were attained in repeated batch fermentation. During seven consecutive batch fermentations, the biocatalyst showed high mechanical and operational stability reaching an overall productivity of 0.78 g L-1 h(-1). This study suggested that the "freezing-thawing" technique can be successfully used for immobilization of L. rhamnosus in PVA/Ca-alginate matrix without loss of either viability or LA fermentation capability.
PB  - Springer, New York
T2  - Bioprocess and Biosystems Engineering
T1  - Immobilization of Lactobacillus rhamnosus in polyvinyl alcohol/calcium alginate matrix for production of lactic acid
EP  - 322
IS  - 2
SP  - 315
VL  - 43
DO  - 10.1007/s00449-019-02228-0
ER  - 

@article{
author = "Radosavljević, Miloš and Lević, Steva and Belović, Miona and Pejin, Jelena and Đukić-Vuković, Aleksandra and Mojović, Ljiljana and Nedović, Viktor",
year = "2020",
abstract = "Immobilization of Lactobacillus rhamnosus ATCC7469 in poly(vinyl alcohol)/calcium alginate (PVA/Ca-alginate) matrix using "freezing-thawing" technique for application in lactic acid (LA) fermentation was studied in this paper. PVA/Ca-alginate beads were made from sterile and non-sterile PVA and sodium alginate solutions. According to mechanical properties, the PVA/Ca-alginate beads expressed a strong elastic character. Obtained PVA/Ca-alginate beads were further applied in batch and repeated batch LA fermentations. Regarding cell viability, L. rhamnosus cells survived well rather sharp immobilization procedure and significant cell proliferation was observed in further fermentation studies achieving high cell viability (up to 10.7 log CFU g(-1)) in sterile beads. In batch LA fermentation, the immobilized biocatalyst was superior to free cell fermentation system (by 37.1%), while the highest LA yield and volumetric productivity of 97.6% and 0.8 g L-1 h(-1), respectively, were attained in repeated batch fermentation. During seven consecutive batch fermentations, the biocatalyst showed high mechanical and operational stability reaching an overall productivity of 0.78 g L-1 h(-1). This study suggested that the "freezing-thawing" technique can be successfully used for immobilization of L. rhamnosus in PVA/Ca-alginate matrix without loss of either viability or LA fermentation capability.",
publisher = "Springer, New York",
journal = "Bioprocess and Biosystems Engineering",
title = "Immobilization of Lactobacillus rhamnosus in polyvinyl alcohol/calcium alginate matrix for production of lactic acid",
pages = "322-315",
number = "2",
volume = "43",
doi = "10.1007/s00449-019-02228-0"
}

Radosavljević, M., Lević, S., Belović, M., Pejin, J., Đukić-Vuković, A., Mojović, L.,& Nedović, V.. (2020). Immobilization of Lactobacillus rhamnosus in polyvinyl alcohol/calcium alginate matrix for production of lactic acid. in Bioprocess and Biosystems Engineering
Springer, New York., 43(2), 315-322.
https://doi.org/10.1007/s00449-019-02228-0

Radosavljević M, Lević S, Belović M, Pejin J, Đukić-Vuković A, Mojović L, Nedović V. Immobilization of Lactobacillus rhamnosus in polyvinyl alcohol/calcium alginate matrix for production of lactic acid. in Bioprocess and Biosystems Engineering. 2020;43(2):315-322.
doi:10.1007/s00449-019-02228-0 .

Radosavljević, Miloš, Lević, Steva, Belović, Miona, Pejin, Jelena, Đukić-Vuković, Aleksandra, Mojović, Ljiljana, Nedović, Viktor, "Immobilization of Lactobacillus rhamnosus in polyvinyl alcohol/calcium alginate matrix for production of lactic acid" in Bioprocess and Biosystems Engineering, 43, no. 2 (2020):315-322,
https://doi.org/10.1007/s00449-019-02228-0 . .

TY  - JOUR
AU  - Spasić, Aleksandar
AU  - Manojlović, Vaso
AU  - Jovanović, Mica
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4422
AB  - Applications of solvent extraction operations and processes play one of the most important roles in Hydrometallurgy. Therefore, in this brief review, some general concepts for selected representative applications are discussed. Also, one particular entrainment problem solution is discussed in some more details. At first, the selected general concepts for metal production of copper and uranium from their ores are presented. Then after, the leaching-solvent extraction-electro winning process for copper is shown. Finally, the extraction of uranium from wet phosphoric acid is discussed.
PB  - Association of Metallurgical Engineers of Serbia
T2  - Metallurgical & Materials Engineering
T1  - Solvent extraction and entrainment problem
EP  - 175
IS  - 2
SP  - 163
VL  - 26
DO  - 10.30544/480
ER  - 

@article{
author = "Spasić, Aleksandar and Manojlović, Vaso and Jovanović, Mica",
year = "2020",
abstract = "Applications of solvent extraction operations and processes play one of the most important roles in Hydrometallurgy. Therefore, in this brief review, some general concepts for selected representative applications are discussed. Also, one particular entrainment problem solution is discussed in some more details. At first, the selected general concepts for metal production of copper and uranium from their ores are presented. Then after, the leaching-solvent extraction-electro winning process for copper is shown. Finally, the extraction of uranium from wet phosphoric acid is discussed.",
publisher = "Association of Metallurgical Engineers of Serbia",
journal = "Metallurgical & Materials Engineering",
title = "Solvent extraction and entrainment problem",
pages = "175-163",
number = "2",
volume = "26",
doi = "10.30544/480"
}

Spasić, A., Manojlović, V.,& Jovanović, M.. (2020). Solvent extraction and entrainment problem. in Metallurgical & Materials Engineering
Association of Metallurgical Engineers of Serbia., 26(2), 163-175.
https://doi.org/10.30544/480

Spasić A, Manojlović V, Jovanović M. Solvent extraction and entrainment problem. in Metallurgical & Materials Engineering. 2020;26(2):163-175.
doi:10.30544/480 .

Spasić, Aleksandar, Manojlović, Vaso, Jovanović, Mica, "Solvent extraction and entrainment problem" in Metallurgical & Materials Engineering, 26, no. 2 (2020):163-175,
https://doi.org/10.30544/480 . .

TY  - JOUR
AU  - Vučković, N.
AU  - Glođović, N.
AU  - Radovanović, Željko
AU  - Janaćković, Đorđe
AU  - Milašinović, Nikola
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4569
AB  - Most chemical and physical methods employed in visualizing latent marks have shown detrimental effects on human health and, therefore, some research groups have directed their attention to the utilization of various (bio)polymers with the aforementioned purpose. Although chitosan is widely used in medicine, pharmacy, food, and drug delivery systems, there are very few studies that address this biopolymer utilization in forensic applications, such as the detection of latent fingerprints. We used chitosan crosslinked with sodium tripolyphosphate and conjugated with L-lysine to enhance the visualization of latent fingerprints, due to its ability to interact with fingerprint sweat residues. These conjugates were prepared using different (w/w) amounts of chitosan and tripolyphosphate (6/1; 4/1; 1/1; 1/4; and 1/6), and those with the most promising results (i.e., 6/1 formulation) were investigated in detail. Fourier transform infrared (FT-IR) spectroscopy confirmed interactions between components of the systems. Optical microscopy and scanning electron microscope (SEM) analysis showed that prepared powder formulations were uniform in size and confirmed that chitosan/tripolyphosphate/lysine conjugates bind easily to the sweat and lipid residues present in the latent fingerprints. The testing of prepared conjugates demonstrated the potential of these systems as bio-based powder substitution for commercially available powders.
T2  - Journal of Forensic Sciences
T1  - A novel chitosan/tripolyphosphate/L-lysine conjugates for latent fingerprints detection and enhancement
DO  - 10.1111/1556-4029.14569
ER  - 

@article{
author = "Vučković, N. and Glođović, N. and Radovanović, Željko and Janaćković, Đorđe and Milašinović, Nikola",
year = "2020",
abstract = "Most chemical and physical methods employed in visualizing latent marks have shown detrimental effects on human health and, therefore, some research groups have directed their attention to the utilization of various (bio)polymers with the aforementioned purpose. Although chitosan is widely used in medicine, pharmacy, food, and drug delivery systems, there are very few studies that address this biopolymer utilization in forensic applications, such as the detection of latent fingerprints. We used chitosan crosslinked with sodium tripolyphosphate and conjugated with L-lysine to enhance the visualization of latent fingerprints, due to its ability to interact with fingerprint sweat residues. These conjugates were prepared using different (w/w) amounts of chitosan and tripolyphosphate (6/1; 4/1; 1/1; 1/4; and 1/6), and those with the most promising results (i.e., 6/1 formulation) were investigated in detail. Fourier transform infrared (FT-IR) spectroscopy confirmed interactions between components of the systems. Optical microscopy and scanning electron microscope (SEM) analysis showed that prepared powder formulations were uniform in size and confirmed that chitosan/tripolyphosphate/lysine conjugates bind easily to the sweat and lipid residues present in the latent fingerprints. The testing of prepared conjugates demonstrated the potential of these systems as bio-based powder substitution for commercially available powders.",
journal = "Journal of Forensic Sciences",
title = "A novel chitosan/tripolyphosphate/L-lysine conjugates for latent fingerprints detection and enhancement",
doi = "10.1111/1556-4029.14569"
}

Vučković, N., Glođović, N., Radovanović, Ž., Janaćković, Đ.,& Milašinović, N.. (2020). A novel chitosan/tripolyphosphate/L-lysine conjugates for latent fingerprints detection and enhancement. in Journal of Forensic Sciences.
https://doi.org/10.1111/1556-4029.14569

Vučković N, Glođović N, Radovanović Ž, Janaćković Đ, Milašinović N. A novel chitosan/tripolyphosphate/L-lysine conjugates for latent fingerprints detection and enhancement. in Journal of Forensic Sciences. 2020;.
doi:10.1111/1556-4029.14569 .

Vučković, N., Glođović, N., Radovanović, Željko, Janaćković, Đorđe, Milašinović, Nikola, "A novel chitosan/tripolyphosphate/L-lysine conjugates for latent fingerprints detection and enhancement" in Journal of Forensic Sciences (2020),
https://doi.org/10.1111/1556-4029.14569 . .

TY  - JOUR
AU  - Karaca, Sara
AU  - Trifković, Kata T.
AU  - Martinić, Arijana
AU  - Đorđević, Verica
AU  - Seremet, Danijela
AU  - Vojvodić-Cebin, Aleksandra
AU  - Bugarski, Branko
AU  - Komes, Drazenka
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4343
AB  - The aim of this study was to develop functional edible films containing cocoa (Theobroma cacao L.) powder extract using alginate, pectin and chitosan in combination with proteins (whey protein isolate, soya and hemp protein). The films were examined for their physico-chemical (dry matter content, colour, thickness), mechanical (elongation at break (EAB)), bioactive (the content of total polyphenols (TPC), flavan-3-ols (F3olC), antioxidant capacity (AC)) and sensory properties. The plain alginate film exhibited the highest EAB (29.1%). The highest TPC and F3olC were determined in plain alginate (29 mg GAE/g and 2.75 mg (+)-catechin/g) and pectin (29 mg GAE/g and 2.25 mg (+)-catechin/g) films. The addition of proteins resulted in prolonged release of polyphenols and enhanced functional properties; however, the formation of protein-polyphenol complexes caused slight alterations in the bioactive composition of the films. The obtained results indicate a high potential of the developed films as functional, biodegradable form of active food packaging.
PB  - Wiley, Hoboken
T2  - International Journal of Food Science and Technology
T1  - Development and characterisation of functional cocoa (Theobroma cacao L.)-based edible films
EP  - 1335
IS  - 3
SP  - 1326
VL  - 55
DO  - 10.1111/ijfs.14407
ER  - 

@article{
author = "Karaca, Sara and Trifković, Kata T. and Martinić, Arijana and Đorđević, Verica and Seremet, Danijela and Vojvodić-Cebin, Aleksandra and Bugarski, Branko and Komes, Drazenka",
year = "2020",
abstract = "The aim of this study was to develop functional edible films containing cocoa (Theobroma cacao L.) powder extract using alginate, pectin and chitosan in combination with proteins (whey protein isolate, soya and hemp protein). The films were examined for their physico-chemical (dry matter content, colour, thickness), mechanical (elongation at break (EAB)), bioactive (the content of total polyphenols (TPC), flavan-3-ols (F3olC), antioxidant capacity (AC)) and sensory properties. The plain alginate film exhibited the highest EAB (29.1%). The highest TPC and F3olC were determined in plain alginate (29 mg GAE/g and 2.75 mg (+)-catechin/g) and pectin (29 mg GAE/g and 2.25 mg (+)-catechin/g) films. The addition of proteins resulted in prolonged release of polyphenols and enhanced functional properties; however, the formation of protein-polyphenol complexes caused slight alterations in the bioactive composition of the films. The obtained results indicate a high potential of the developed films as functional, biodegradable form of active food packaging.",
publisher = "Wiley, Hoboken",
journal = "International Journal of Food Science and Technology",
title = "Development and characterisation of functional cocoa (Theobroma cacao L.)-based edible films",
pages = "1335-1326",
number = "3",
volume = "55",
doi = "10.1111/ijfs.14407"
}

Karaca, S., Trifković, K. T., Martinić, A., Đorđević, V., Seremet, D., Vojvodić-Cebin, A., Bugarski, B.,& Komes, D.. (2020). Development and characterisation of functional cocoa (Theobroma cacao L.)-based edible films. in International Journal of Food Science and Technology
Wiley, Hoboken., 55(3), 1326-1335.
https://doi.org/10.1111/ijfs.14407

Karaca S, Trifković KT, Martinić A, Đorđević V, Seremet D, Vojvodić-Cebin A, Bugarski B, Komes D. Development and characterisation of functional cocoa (Theobroma cacao L.)-based edible films. in International Journal of Food Science and Technology. 2020;55(3):1326-1335.
doi:10.1111/ijfs.14407 .

Karaca, Sara, Trifković, Kata T., Martinić, Arijana, Đorđević, Verica, Seremet, Danijela, Vojvodić-Cebin, Aleksandra, Bugarski, Branko, Komes, Drazenka, "Development and characterisation of functional cocoa (Theobroma cacao L.)-based edible films" in International Journal of Food Science and Technology, 55, no. 3 (2020):1326-1335,
https://doi.org/10.1111/ijfs.14407 . .

TY  - CONF
AU  - Bošković, Stefan A.
AU  - Bugarski, Branko
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7365
AB  - The electrospray process is a process that uses an electric field to influence the liquid dripping out of a capillary tube in order to obtain a reduction in the mean droplet diameter. The theory regarding this process is called electrohydrodynamics. Based on the previous experimental results and on the published theories, important variables are listed. Some of those variables have a significant influence on the droplet diameter, while others should be controlled in order to check the applicability of the mentioned theories. Since theoretical equations have a simple power-type form, that form was used for obtaining a correlation of particle diameters when alginate solutions are used. Previous experimental data and a Generalized Reduced Gradient (GRG) nonlinear solver were used for calculating the coefficients present in the correlation. Interesting results were obtained because certain variables were eliminated during the calculation. Based on the presented analysis, future experiments should monitor more variables and computational fluid dynamics (CFD) should also be used.
PB  - Zvornik : Faculty of Technology
C3  - Proceedings / VI International Congress "Engineering, Ecology and Materials in the Processing Industry", Jahorina, March 11-13, 2019
T1  - Correlation of particle diameters and determination of important variables for particles obtained by using the electrospray process and alginate solutions
EP  - 259
SP  - 255
DO  - 10.7251/EEMEN1901255B
ER  - 

@conference{
author = "Bošković, Stefan A. and Bugarski, Branko",
year = "2019",
abstract = "The electrospray process is a process that uses an electric field to influence the liquid dripping out of a capillary tube in order to obtain a reduction in the mean droplet diameter. The theory regarding this process is called electrohydrodynamics. Based on the previous experimental results and on the published theories, important variables are listed. Some of those variables have a significant influence on the droplet diameter, while others should be controlled in order to check the applicability of the mentioned theories. Since theoretical equations have a simple power-type form, that form was used for obtaining a correlation of particle diameters when alginate solutions are used. Previous experimental data and a Generalized Reduced Gradient (GRG) nonlinear solver were used for calculating the coefficients present in the correlation. Interesting results were obtained because certain variables were eliminated during the calculation. Based on the presented analysis, future experiments should monitor more variables and computational fluid dynamics (CFD) should also be used.",
publisher = "Zvornik : Faculty of Technology",
journal = "Proceedings / VI International Congress "Engineering, Ecology and Materials in the Processing Industry", Jahorina, March 11-13, 2019",
title = "Correlation of particle diameters and determination of important variables for particles obtained by using the electrospray process and alginate solutions",
pages = "259-255",
doi = "10.7251/EEMEN1901255B"
}

Bošković, S. A.,& Bugarski, B.. (2019). Correlation of particle diameters and determination of important variables for particles obtained by using the electrospray process and alginate solutions. in Proceedings / VI International Congress "Engineering, Ecology and Materials in the Processing Industry", Jahorina, March 11-13, 2019
Zvornik : Faculty of Technology., 255-259.
https://doi.org/10.7251/EEMEN1901255B

Bošković SA, Bugarski B. Correlation of particle diameters and determination of important variables for particles obtained by using the electrospray process and alginate solutions. in Proceedings / VI International Congress "Engineering, Ecology and Materials in the Processing Industry", Jahorina, March 11-13, 2019. 2019;:255-259.
doi:10.7251/EEMEN1901255B .

Bošković, Stefan A., Bugarski, Branko, "Correlation of particle diameters and determination of important variables for particles obtained by using the electrospray process and alginate solutions" in Proceedings / VI International Congress "Engineering, Ecology and Materials in the Processing Industry", Jahorina, March 11-13, 2019 (2019):255-259,
https://doi.org/10.7251/EEMEN1901255B . .

TY  - CHAP
AU  - Milivojević, Milan
AU  - Pajić-Lijaković, Ivana
AU  - Bugarski, Branko
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7428
AB  - Alginates as natural, anionic polymers have many properties that make
them very attractive for applications in many different industries. Most
advantageous characteristics that make alginates so widely used are their
low cost, biocompatibility, low toxicity, and especially, easy gel forming in
mild conditions. In addition, an abundance of carboxyl and hydroxyl groups
enables easy chemical or biochemical modification which may give the
further improvement of their properties to tailor some specific demands, or
to eliminate some of their disadvantages. Biomedical applications of alginates mainly include encapsulations for drug delivery and controlled release,
tablet formulation, wound healing, as a scaffold for tissue engineering,
dental impression materials, water absorbents, chelators, and so on. Their
use in this field is attributed to their nonimmunogenicity and hydrophilicity, the simple formulation in any form (such as capsule, film, bead, or fiber),
and easy blending with other polymers. However, application of alginates
for modern biomedical treatments demand a comprehensive understanding
of their properties, broad knowledge about the relationship between their
structure and their functions, as well as, clear insight in possibilities for their
chemical or biochemical methods of modifications. This chapter is focused
on recent advances related to the applications, research, and development
of the biomedical use of alginate and survey of possible chemical and
biochemical means for their modifications.
PB  - New York : Apple Academic Press
PB  - CRC Press, Taylor & Francis Group
T2  - Alginates: Versatile Polymers in Biomedical Applications and Therapeutics
T1  - Recent Advances in Alginates as Material for Biomedical Applications
EP  - 87
SP  - 25
DO  - 10.1201/9780429023439-2
ER  - 

@inbook{
author = "Milivojević, Milan and Pajić-Lijaković, Ivana and Bugarski, Branko",
year = "2019",
abstract = "Alginates as natural, anionic polymers have many properties that make
them very attractive for applications in many different industries. Most
advantageous characteristics that make alginates so widely used are their
low cost, biocompatibility, low toxicity, and especially, easy gel forming in
mild conditions. In addition, an abundance of carboxyl and hydroxyl groups
enables easy chemical or biochemical modification which may give the
further improvement of their properties to tailor some specific demands, or
to eliminate some of their disadvantages. Biomedical applications of alginates mainly include encapsulations for drug delivery and controlled release,
tablet formulation, wound healing, as a scaffold for tissue engineering,
dental impression materials, water absorbents, chelators, and so on. Their
use in this field is attributed to their nonimmunogenicity and hydrophilicity, the simple formulation in any form (such as capsule, film, bead, or fiber),
and easy blending with other polymers. However, application of alginates
for modern biomedical treatments demand a comprehensive understanding
of their properties, broad knowledge about the relationship between their
structure and their functions, as well as, clear insight in possibilities for their
chemical or biochemical methods of modifications. This chapter is focused
on recent advances related to the applications, research, and development
of the biomedical use of alginate and survey of possible chemical and
biochemical means for their modifications.",
publisher = "New York : Apple Academic Press, CRC Press, Taylor & Francis Group",
journal = "Alginates: Versatile Polymers in Biomedical Applications and Therapeutics",
booktitle = "Recent Advances in Alginates as Material for Biomedical Applications",
pages = "87-25",
doi = "10.1201/9780429023439-2"
}

Milivojević, M., Pajić-Lijaković, I.,& Bugarski, B.. (2019). Recent Advances in Alginates as Material for Biomedical Applications. in Alginates: Versatile Polymers in Biomedical Applications and Therapeutics
New York : Apple Academic Press., 25-87.
https://doi.org/10.1201/9780429023439-2

Milivojević M, Pajić-Lijaković I, Bugarski B. Recent Advances in Alginates as Material for Biomedical Applications. in Alginates: Versatile Polymers in Biomedical Applications and Therapeutics. 2019;:25-87.
doi:10.1201/9780429023439-2 .

Milivojević, Milan, Pajić-Lijaković, Ivana, Bugarski, Branko, "Recent Advances in Alginates as Material for Biomedical Applications" in Alginates: Versatile Polymers in Biomedical Applications and Therapeutics (2019):25-87,
https://doi.org/10.1201/9780429023439-2 . .

TY  - CONF
AU  - Marković, Maja D.
AU  - Seslija, Sanja I.
AU  - Panić, Vesna V.
AU  - Pjanović, Rada V.
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5074
AB  - Three dimensional polymer networks, hydrogels, have shown a great potential in
controlled release and targeted delivery of active substances. pH sensitive hydrogels
based on poly(methacrylic acid) have attracted significant attention due to their
biocompatibility, non-toxicity and the possession of the huge number of ionogenic
carboxylic groups. On the other hand, the usage of these hydrogels for controlled release
and targeted delivery of poorly water-soluble drugs is limited by their highly hydrophilic
nature. We overcame this limitation by modifying PMAA with amphiphilic substance -
casein. The addition of this natural protein enabled the encapsulation, targeted delivery
and controlled release of poorly water-soluble model drug - caffeine. Three carriers with
different neutralization degree of methacrylic acid were synthesized via free radical
polymerization. The FTIR spectra revealed that two types of interactions were
established between casein and caffeine: hydrophobic interactions and hydrogen bonds.
The SEM micrographs showed that the structure of the carriers depended on the change
of neutralization degree of the methacrylic acid. The swelling of synthesized carriers and
the caffeine in vitro release were monitored in two environments at 37°C: 0.1M HCl
pH=1.2 (simulation of environment in human stomach) and phosphate buffer pH=6.8
(simulation of environment in human intestines). The synthesized carriers had higher
swelling degree and the higher release rate of caffeine in the phosphate buffer pH=6.8
than in 0.1M HCl. The increase in neutralization degree of methacrylic acid caused the
increase in swelling degree of the carriers and the increase in release rate of caffeine.
Presented results showed that synthesized carriers are promising candidates for targeted
delivery of poorly water-soluble drugs.
PB  - Serbian Chemical Society and Serbian Young Chemists Club Karnegijeva 4/III, 11000 Belgrade, Serbia
C3  - Conference of the Young Chemists of Serbia
T1  - Three dimensional polymeric networks based on poly(methacrylic acid) and protein for targeted delivery of poorly water-soluble drugs
SP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5074
ER  - 

@conference{
author = "Marković, Maja D. and Seslija, Sanja I. and Panić, Vesna V. and Pjanović, Rada V.",
year = "2019",
abstract = "Three dimensional polymer networks, hydrogels, have shown a great potential in
controlled release and targeted delivery of active substances. pH sensitive hydrogels
based on poly(methacrylic acid) have attracted significant attention due to their
biocompatibility, non-toxicity and the possession of the huge number of ionogenic
carboxylic groups. On the other hand, the usage of these hydrogels for controlled release
and targeted delivery of poorly water-soluble drugs is limited by their highly hydrophilic
nature. We overcame this limitation by modifying PMAA with amphiphilic substance -
casein. The addition of this natural protein enabled the encapsulation, targeted delivery
and controlled release of poorly water-soluble model drug - caffeine. Three carriers with
different neutralization degree of methacrylic acid were synthesized via free radical
polymerization. The FTIR spectra revealed that two types of interactions were
established between casein and caffeine: hydrophobic interactions and hydrogen bonds.
The SEM micrographs showed that the structure of the carriers depended on the change
of neutralization degree of the methacrylic acid. The swelling of synthesized carriers and
the caffeine in vitro release were monitored in two environments at 37°C: 0.1M HCl
pH=1.2 (simulation of environment in human stomach) and phosphate buffer pH=6.8
(simulation of environment in human intestines). The synthesized carriers had higher
swelling degree and the higher release rate of caffeine in the phosphate buffer pH=6.8
than in 0.1M HCl. The increase in neutralization degree of methacrylic acid caused the
increase in swelling degree of the carriers and the increase in release rate of caffeine.
Presented results showed that synthesized carriers are promising candidates for targeted
delivery of poorly water-soluble drugs.",
publisher = "Serbian Chemical Society and Serbian Young Chemists Club Karnegijeva 4/III, 11000 Belgrade, Serbia",
journal = "Conference of the Young Chemists of Serbia",
title = "Three dimensional polymeric networks based on poly(methacrylic acid) and protein for targeted delivery of poorly water-soluble drugs",
pages = "134",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5074"
}

Marković, M. D., Seslija, S. I., Panić, V. V.,& Pjanović, R. V.. (2019). Three dimensional polymeric networks based on poly(methacrylic acid) and protein for targeted delivery of poorly water-soluble drugs. in Conference of the Young Chemists of Serbia
Serbian Chemical Society and Serbian Young Chemists Club Karnegijeva 4/III, 11000 Belgrade, Serbia., 134.
https://hdl.handle.net/21.15107/rcub_technorep_5074

Marković MD, Seslija SI, Panić VV, Pjanović RV. Three dimensional polymeric networks based on poly(methacrylic acid) and protein for targeted delivery of poorly water-soluble drugs. in Conference of the Young Chemists of Serbia. 2019;:134.
https://hdl.handle.net/21.15107/rcub_technorep_5074 .

Marković, Maja D., Seslija, Sanja I., Panić, Vesna V., Pjanović, Rada V., "Three dimensional polymeric networks based on poly(methacrylic acid) and protein for targeted delivery of poorly water-soluble drugs" in Conference of the Young Chemists of Serbia (2019):134,
https://hdl.handle.net/21.15107/rcub_technorep_5074 .

TY  - JOUR
AU  - Petrović, Predrag
AU  - Ivanović, Katarina
AU  - Octrue, Charly
AU  - Tumara, Mica
AU  - Jovanović, Aleksandra
AU  - Vunduk, Jovana
AU  - Nikšić, Miomir
AU  - Pjanović, Rada
AU  - Bugarski, Branko
AU  - Klaus, Anita
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4105
AB  - Chaga (Inonotus obliquus) is a parasitic fungus, which has been used in traditional medicine in Russia and other northern European countries in the treatment of cancer, gastrointestinal andliver diseases. It has been a subject of intensive researchrecently, confirming many of its health-beneficial effects. In order to obtain a product that would allow modified and prolonged release of the Chaga's active metabolites, hot water Chaga extract was immobilized using calcium - alginate. The extract, which was predominantly composed of carbohydrates (57 %), also contained a relatively high amount of antioxidants/phenolic compounds (130 mg gallic acid equivalents per g of dry extract) and exhibited pronounced radical scavenging activity. It showed significant antibacterial activity as well, inhibiting growth of tested bacterial strains at concentrations of 1.25-20 mg/mL. Entrapment efficiency was about 80 %, and the extract-alginate system showed pH-dependant extract release; there was negligible release at pH 1.75 (gastric pH), and the release gradually increased with the increase in pH, reaching similar to 43 % of immobilized extract at pH 8.5 after 90 min. Such a product could be used as a dietary supplement, adjuvant in therapy of gastrointestinal diseases or as a food additive.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Immobilization of Chaga extract in alginate beads for modified release: simplicity meets efficiency
EP  - 335
IS  - 5
SP  - 325
VL  - 73
DO  - 10.2298/HEMIND190819028P
ER  - 

@article{
author = "Petrović, Predrag and Ivanović, Katarina and Octrue, Charly and Tumara, Mica and Jovanović, Aleksandra and Vunduk, Jovana and Nikšić, Miomir and Pjanović, Rada and Bugarski, Branko and Klaus, Anita",
year = "2019",
abstract = "Chaga (Inonotus obliquus) is a parasitic fungus, which has been used in traditional medicine in Russia and other northern European countries in the treatment of cancer, gastrointestinal andliver diseases. It has been a subject of intensive researchrecently, confirming many of its health-beneficial effects. In order to obtain a product that would allow modified and prolonged release of the Chaga's active metabolites, hot water Chaga extract was immobilized using calcium - alginate. The extract, which was predominantly composed of carbohydrates (57 %), also contained a relatively high amount of antioxidants/phenolic compounds (130 mg gallic acid equivalents per g of dry extract) and exhibited pronounced radical scavenging activity. It showed significant antibacterial activity as well, inhibiting growth of tested bacterial strains at concentrations of 1.25-20 mg/mL. Entrapment efficiency was about 80 %, and the extract-alginate system showed pH-dependant extract release; there was negligible release at pH 1.75 (gastric pH), and the release gradually increased with the increase in pH, reaching similar to 43 % of immobilized extract at pH 8.5 after 90 min. Such a product could be used as a dietary supplement, adjuvant in therapy of gastrointestinal diseases or as a food additive.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Immobilization of Chaga extract in alginate beads for modified release: simplicity meets efficiency",
pages = "335-325",
number = "5",
volume = "73",
doi = "10.2298/HEMIND190819028P"
}

Petrović, P., Ivanović, K., Octrue, C., Tumara, M., Jovanović, A., Vunduk, J., Nikšić, M., Pjanović, R., Bugarski, B.,& Klaus, A.. (2019). Immobilization of Chaga extract in alginate beads for modified release: simplicity meets efficiency. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 73(5), 325-335.
https://doi.org/10.2298/HEMIND190819028P

Petrović P, Ivanović K, Octrue C, Tumara M, Jovanović A, Vunduk J, Nikšić M, Pjanović R, Bugarski B, Klaus A. Immobilization of Chaga extract in alginate beads for modified release: simplicity meets efficiency. in Hemijska industrija. 2019;73(5):325-335.
doi:10.2298/HEMIND190819028P .

Petrović, Predrag, Ivanović, Katarina, Octrue, Charly, Tumara, Mica, Jovanović, Aleksandra, Vunduk, Jovana, Nikšić, Miomir, Pjanović, Rada, Bugarski, Branko, Klaus, Anita, "Immobilization of Chaga extract in alginate beads for modified release: simplicity meets efficiency" in Hemijska industrija, 73, no. 5 (2019):325-335,
https://doi.org/10.2298/HEMIND190819028P . .

TY  - JOUR
AU  - Petrović, Predrag
AU  - Ivanović, Katarina
AU  - Jovanović, Aleksandra
AU  - Simović, Milica
AU  - Milutinović, Violeta
AU  - Kozarski, Maja
AU  - Petković, Miloš
AU  - Cvetković, Anka
AU  - Klaus, Anita
AU  - Bugarski, Branko
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4122
AB  - Puffballs are fungi that produce globose fruiting bodies that undergo autolysis, transforming their insides into a spore bearing, powdery mass. Mature fruiting bodies are traditionally used to treat open skin wounds. In this study, methanol extracts of two puffball species, Handkea excipuliformis and Vascellum pratense, were examined and compared in order to provide insight into the changes these mushrooms undergo during maturation, with respect to their potential use in skin care and wound treatment. Some compounds involved in skin care and regeneration were quantified, and it was found that maturation increases the concentrations of almost all of these compounds. Antioxidant activity was also more pronounced in mature fruiting body extracts, which was in correlation with the higher content of antioxidants. Tyrosinase inhibition was vastly improved with autolysis, correlating with the higher phenolic content in mature fruiting body extracts. Antimicrobial activity was negatively affected by autolysis in the case of H. excipuliformis, whereas autolysis had little effect on the antimicrobial activity of V. pratense. Autolysis generally improved the biological activity and increased the concentrations of compounds involved in skin care, which justifies the traditional use of puffballs and makes them good candidates for various potential cosmetic and medicinal skin-care products.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products
EP  - 733
IS  - 4
SP  - 721
VL  - 71
DO  - 10.2298/ABS190725055P
ER  - 

@article{
author = "Petrović, Predrag and Ivanović, Katarina and Jovanović, Aleksandra and Simović, Milica and Milutinović, Violeta and Kozarski, Maja and Petković, Miloš and Cvetković, Anka and Klaus, Anita and Bugarski, Branko",
year = "2019",
abstract = "Puffballs are fungi that produce globose fruiting bodies that undergo autolysis, transforming their insides into a spore bearing, powdery mass. Mature fruiting bodies are traditionally used to treat open skin wounds. In this study, methanol extracts of two puffball species, Handkea excipuliformis and Vascellum pratense, were examined and compared in order to provide insight into the changes these mushrooms undergo during maturation, with respect to their potential use in skin care and wound treatment. Some compounds involved in skin care and regeneration were quantified, and it was found that maturation increases the concentrations of almost all of these compounds. Antioxidant activity was also more pronounced in mature fruiting body extracts, which was in correlation with the higher content of antioxidants. Tyrosinase inhibition was vastly improved with autolysis, correlating with the higher phenolic content in mature fruiting body extracts. Antimicrobial activity was negatively affected by autolysis in the case of H. excipuliformis, whereas autolysis had little effect on the antimicrobial activity of V. pratense. Autolysis generally improved the biological activity and increased the concentrations of compounds involved in skin care, which justifies the traditional use of puffballs and makes them good candidates for various potential cosmetic and medicinal skin-care products.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products",
pages = "733-721",
number = "4",
volume = "71",
doi = "10.2298/ABS190725055P"
}

Petrović, P., Ivanović, K., Jovanović, A., Simović, M., Milutinović, V., Kozarski, M., Petković, M., Cvetković, A., Klaus, A.,& Bugarski, B.. (2019). The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 71(4), 721-733.
https://doi.org/10.2298/ABS190725055P

Petrović P, Ivanović K, Jovanović A, Simović M, Milutinović V, Kozarski M, Petković M, Cvetković A, Klaus A, Bugarski B. The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products. in Archives of Biological Sciences. 2019;71(4):721-733.
doi:10.2298/ABS190725055P .

Petrović, Predrag, Ivanović, Katarina, Jovanović, Aleksandra, Simović, Milica, Milutinović, Violeta, Kozarski, Maja, Petković, Miloš, Cvetković, Anka, Klaus, Anita, Bugarski, Branko, "The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products" in Archives of Biological Sciences, 71, no. 4 (2019):721-733,
https://doi.org/10.2298/ABS190725055P . .

TY  - JOUR
AU  - Petrović, Predrag
AU  - Vunduk, Jovana
AU  - Klaus, Anita
AU  - Carević, Momir
AU  - Petković, Miloš
AU  - Vuković, N.
AU  - Cvetković, A.
AU  - Žižak, Željko
AU  - Bugarski, Branko
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4218
AB  - Methanol extracts of mosaic puffball (Handkea utriformis, Bovistella utriformis, Lycoperdon utriforme, Calvatia utriformis - current name is a subject of debate), from three different stages - mycelium (HUMIC), immature (HUI) and mature fruiting bodies (HUM) were characterized and tested for antioxidant, antimicrobial and inhibitory activity on tyrosinase, acetyholinesterase (AChE), butyrylcholinesterase (BuChE) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA-R). Immature, edible, fruiting bodies were shown to be a good source of antioxidants (11.5 mg/g of extract) and cholesterol-lowering agent, lovastatin (234 mu g/g of extract), and exhibited significant antimicrobial activity. In addition, HUI showed good and selective AChE (4.48 mg/mL) and non-lovastatin related HMG-CoA-R inhibition (1.16 mg/mL), which all together suggests that regular consumption of it may have health benefits. Mature fruiting bodies, inedible due to powdery consistence, have been used in traditional medicine for wound treatment; their extract was relatively rich in free ergosterol (31.65 mg/g of extract), N-acetylglucosamine (24 mg/g of extract), alpha-tocopherol (4 mg/g of extract) and had best overall antioxidant activity, which was in correlation with its highest phenolic content (19.4 mg GAE/mL). It also exhibited significant tyrosinase inhibitory activity (0.22 mg/mL) and thus could be used in medicinal and cosmetic products for wound healing and bleaching. Mycelium, which can be easily grown in laboratory conditions did not have the same properties as, neither immature or mature fruiting bodies, although it showed prominent antimicrobial activity, notably against Pseudomonas aeruginosa (MIC = 0.0625 mg/mL) and could be a source of antimicrobial compounds.
PB  - Elsevier Science Bv, Amsterdam
T2  - South African Journal of Botany
T1  - From mycelium to spores: A whole circle of biological potency of mosaic puffball
EP  - 160
SP  - 152
VL  - 123
DO  - 10.1016/j.sajb.2019.03.016
ER  - 

@article{
author = "Petrović, Predrag and Vunduk, Jovana and Klaus, Anita and Carević, Momir and Petković, Miloš and Vuković, N. and Cvetković, A. and Žižak, Željko and Bugarski, Branko",
year = "2019",
abstract = "Methanol extracts of mosaic puffball (Handkea utriformis, Bovistella utriformis, Lycoperdon utriforme, Calvatia utriformis - current name is a subject of debate), from three different stages - mycelium (HUMIC), immature (HUI) and mature fruiting bodies (HUM) were characterized and tested for antioxidant, antimicrobial and inhibitory activity on tyrosinase, acetyholinesterase (AChE), butyrylcholinesterase (BuChE) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA-R). Immature, edible, fruiting bodies were shown to be a good source of antioxidants (11.5 mg/g of extract) and cholesterol-lowering agent, lovastatin (234 mu g/g of extract), and exhibited significant antimicrobial activity. In addition, HUI showed good and selective AChE (4.48 mg/mL) and non-lovastatin related HMG-CoA-R inhibition (1.16 mg/mL), which all together suggests that regular consumption of it may have health benefits. Mature fruiting bodies, inedible due to powdery consistence, have been used in traditional medicine for wound treatment; their extract was relatively rich in free ergosterol (31.65 mg/g of extract), N-acetylglucosamine (24 mg/g of extract), alpha-tocopherol (4 mg/g of extract) and had best overall antioxidant activity, which was in correlation with its highest phenolic content (19.4 mg GAE/mL). It also exhibited significant tyrosinase inhibitory activity (0.22 mg/mL) and thus could be used in medicinal and cosmetic products for wound healing and bleaching. Mycelium, which can be easily grown in laboratory conditions did not have the same properties as, neither immature or mature fruiting bodies, although it showed prominent antimicrobial activity, notably against Pseudomonas aeruginosa (MIC = 0.0625 mg/mL) and could be a source of antimicrobial compounds.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "South African Journal of Botany",
title = "From mycelium to spores: A whole circle of biological potency of mosaic puffball",
pages = "160-152",
volume = "123",
doi = "10.1016/j.sajb.2019.03.016"
}

Petrović, P., Vunduk, J., Klaus, A., Carević, M., Petković, M., Vuković, N., Cvetković, A., Žižak, Ž.,& Bugarski, B.. (2019). From mycelium to spores: A whole circle of biological potency of mosaic puffball. in South African Journal of Botany
Elsevier Science Bv, Amsterdam., 123, 152-160.
https://doi.org/10.1016/j.sajb.2019.03.016

Petrović P, Vunduk J, Klaus A, Carević M, Petković M, Vuković N, Cvetković A, Žižak Ž, Bugarski B. From mycelium to spores: A whole circle of biological potency of mosaic puffball. in South African Journal of Botany. 2019;123:152-160.
doi:10.1016/j.sajb.2019.03.016 .

Petrović, Predrag, Vunduk, Jovana, Klaus, Anita, Carević, Momir, Petković, Miloš, Vuković, N., Cvetković, A., Žižak, Željko, Bugarski, Branko, "From mycelium to spores: A whole circle of biological potency of mosaic puffball" in South African Journal of Botany, 123 (2019):152-160,
https://doi.org/10.1016/j.sajb.2019.03.016 . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Skrt, Mihaela
AU  - Petrović, Predrag
AU  - Janković-Častvan, Ivona
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4288
AB  - The aim of the present study was the optimization of the extraction conditions (particle size, solid-to-solvent ratio, extraction time and procedure) via total polyphenol content and radical scavenging activity, using wild thyme (Thymus serpyllum L.) as a natural source of polyphenols. According to the total polyphenols yield, particle size, solvent-to-solid ratio, and extraction technique have shown statistically significant influence, whereas the exposure time has not been a relevant factor. In all three extraction procedures (maceration, heat-and ultrasound-assisted extraction), the highest polyphenols yield was reached using the smallest plant particles and the highest solvent-to-solid ratio. Moreover, polyphenols content was significantly higher after ultrasound-assisted extraction, in comparison to the extraction at room and high temperature. According to the antioxidant recovery, the influence of different extraction parameters varied depending on the applied antioxidant tests and extraction procedures. 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)-ABTS radical scavenging capacity of extracts followed the next trend: maceration and ultrasound-assisted extraction  gt  heat-assisted extraction , while in 2,2-diphenyl-1-picrylhydrazyl-DPPH neutralization: ultrasound-assisted extraction  gt  heat-assisted extraction  gt  gt  maceration. The study represents an important step in the formulation of antioxidant-rich extracts with potential use in food, pharmaceutical, and cosmetic industry.
PB  - Institute for Medicinal Plants Research "Dr. Josif Pancic"
T2  - Lekovite sirovine
T1  - Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity
EP  - 29
IS  - 39
SP  - 23
DO  - 10.5937/leksir1939023J
ER  - 

@article{
author = "Jovanović, Aleksandra and Skrt, Mihaela and Petrović, Predrag and Janković-Častvan, Ivona and Zdunić, Gordana and Šavikin, Katarina and Bugarski, Branko",
year = "2019",
abstract = "The aim of the present study was the optimization of the extraction conditions (particle size, solid-to-solvent ratio, extraction time and procedure) via total polyphenol content and radical scavenging activity, using wild thyme (Thymus serpyllum L.) as a natural source of polyphenols. According to the total polyphenols yield, particle size, solvent-to-solid ratio, and extraction technique have shown statistically significant influence, whereas the exposure time has not been a relevant factor. In all three extraction procedures (maceration, heat-and ultrasound-assisted extraction), the highest polyphenols yield was reached using the smallest plant particles and the highest solvent-to-solid ratio. Moreover, polyphenols content was significantly higher after ultrasound-assisted extraction, in comparison to the extraction at room and high temperature. According to the antioxidant recovery, the influence of different extraction parameters varied depending on the applied antioxidant tests and extraction procedures. 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)-ABTS radical scavenging capacity of extracts followed the next trend: maceration and ultrasound-assisted extraction  gt  heat-assisted extraction , while in 2,2-diphenyl-1-picrylhydrazyl-DPPH neutralization: ultrasound-assisted extraction  gt  heat-assisted extraction  gt  gt  maceration. The study represents an important step in the formulation of antioxidant-rich extracts with potential use in food, pharmaceutical, and cosmetic industry.",
publisher = "Institute for Medicinal Plants Research "Dr. Josif Pancic"",
journal = "Lekovite sirovine",
title = "Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity",
pages = "29-23",
number = "39",
doi = "10.5937/leksir1939023J"
}

Jovanović, A., Skrt, M., Petrović, P., Janković-Častvan, I., Zdunić, G., Šavikin, K.,& Bugarski, B.. (2019). Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity. in Lekovite sirovine
Institute for Medicinal Plants Research "Dr. Josif Pancic".(39), 23-29.
https://doi.org/10.5937/leksir1939023J

Jovanović A, Skrt M, Petrović P, Janković-Častvan I, Zdunić G, Šavikin K, Bugarski B. Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity. in Lekovite sirovine. 2019;(39):23-29.
doi:10.5937/leksir1939023J .

Jovanović, Aleksandra, Skrt, Mihaela, Petrović, Predrag, Janković-Častvan, Ivona, Zdunić, Gordana, Šavikin, Katarina, Bugarski, Branko, "Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity" in Lekovite sirovine, no. 39 (2019):23-29,
https://doi.org/10.5937/leksir1939023J . .

TY  - THES
AU  - Petrović, Predrag
PY  - 2019
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7308
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:21162/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51893775
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4684
AB  - Fungi are source of various pharmacologiacally active compounds and they have been a subject of extensive study in a past few decades. Medicinal mushrooms were used in traditional medicine in many parts of the world, especially in the far east, and such use goes back to few thousand years. Modern analytical technologies allowed the characterisation of the medicinal proteperties of mushrooms. Still, many fungi species remain unsufficiently studied...
AB  - Gljive su izvor raznih farmakološki aktvinih komponenti zahvaljujući čemu su postale predmet ospežnih istraživanja u poslednjih nekoliko decenija. Lekovite gljive korsitili su mnogi narodi širom sveta, a naročito dalekog istoka, hiljadama godina, ali je tek s razvojem analitike bilo moguće okarakterisati njihovo dejstvo. Ipak, mnoge gljive i dalje su ostale relativno nepoznate u pogledu svog farmakološkog potencijala.
PB  - Univerzitet u Beogradu, Tehnološko-metalurški fakultet
T1  - Characterisation and incapsulation of biologically active components from Fungi handkea utriformis (bull.) kreisel, Handkea excipuliformis (bull.) Kreisel and Vascellum pratense (pers.) Kreisel
T1  - Karakterizacija i inkapsulacija biološki aktivnih komponenti iz gljiva Handkea utriformis (bull.) Kreisel, Handkea excipuliformis (bull.) Kreisel i Vascellum pratense (pers.) Kreisel
UR  - https://hdl.handle.net/21.15107/rcub_technorep_4684
ER  - 

@phdthesis{
author = "Petrović, Predrag",
year = "2019",
abstract = "Fungi are source of various pharmacologiacally active compounds and they have been a subject of extensive study in a past few decades. Medicinal mushrooms were used in traditional medicine in many parts of the world, especially in the far east, and such use goes back to few thousand years. Modern analytical technologies allowed the characterisation of the medicinal proteperties of mushrooms. Still, many fungi species remain unsufficiently studied..., Gljive su izvor raznih farmakološki aktvinih komponenti zahvaljujući čemu su postale predmet ospežnih istraživanja u poslednjih nekoliko decenija. Lekovite gljive korsitili su mnogi narodi širom sveta, a naročito dalekog istoka, hiljadama godina, ali je tek s razvojem analitike bilo moguće okarakterisati njihovo dejstvo. Ipak, mnoge gljive i dalje su ostale relativno nepoznate u pogledu svog farmakološkog potencijala.",
publisher = "Univerzitet u Beogradu, Tehnološko-metalurški fakultet",
title = "Characterisation and incapsulation of biologically active components from Fungi handkea utriformis (bull.) kreisel, Handkea excipuliformis (bull.) Kreisel and Vascellum pratense (pers.) Kreisel, Karakterizacija i inkapsulacija biološki aktivnih komponenti iz gljiva Handkea utriformis (bull.) Kreisel, Handkea excipuliformis (bull.) Kreisel i Vascellum pratense (pers.) Kreisel",
url = "https://hdl.handle.net/21.15107/rcub_technorep_4684"
}

Petrović, P.. (2019). Characterisation and incapsulation of biologically active components from Fungi handkea utriformis (bull.) kreisel, Handkea excipuliformis (bull.) Kreisel and Vascellum pratense (pers.) Kreisel. 
Univerzitet u Beogradu, Tehnološko-metalurški fakultet..
https://hdl.handle.net/21.15107/rcub_technorep_4684

Petrović P. Characterisation and incapsulation of biologically active components from Fungi handkea utriformis (bull.) kreisel, Handkea excipuliformis (bull.) Kreisel and Vascellum pratense (pers.) Kreisel. 2019;.
https://hdl.handle.net/21.15107/rcub_technorep_4684 .

Petrović, Predrag, "Characterisation and incapsulation of biologically active components from Fungi handkea utriformis (bull.) kreisel, Handkea excipuliformis (bull.) Kreisel and Vascellum pratense (pers.) Kreisel" (2019),
https://hdl.handle.net/21.15107/rcub_technorep_4684 .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Petrović, Predrag M.
AU  - Đorđević, Verica B.
AU  - Častvan, Ivona M.
AU  - Lević, Steva M.
AU  - Zdunić, Gordana M.
AU  - Bugarski, Branko M.
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6457
AB  - In the present study, spray-dried Thymus serpyllum extracts obtained by using four extraction
techniques (maceration, heat-assisted extraction, extraction in ultrasound bath and extraction by
ultrasound probe) were characterized through determination of total polyphenols and total
flavonoids contents, antioxidant capacity, zeta potential, bulk density and solubility. Polyphenols
yield was statistically higher in the extract obtained by ultrasound probe, in comparison to the
extracts prepared using maceration, heat-assisted extraction and extraction in ultrasound bath.
On the other hand, extraction technique did not show statistically significant influence on the
flavonoids concentration. Furthermore, extraction procedures did not show significant effect on
antioxidant neutralization of DPPH radicals, while flavonoids yield had correlation with the
DPPH radical-scavenging capacity assay. However, the extracts obtained using maceration and
ultrasound waves (ultrasound bath) have shown significantly better antioxidant capacity in the
preservation of β-carotene, compared to the extracts prepared by using high temperature and
ultrasound probe. The extract obtained in maceration has shown statistically better stability
(higher absolute value of zeta potential), compared to the extracts prepared using high
temperature and ultrasound waves. According to the results of Pharmacopoeia test, bulk density
of spray-dried T. serpyllum extracts was ranked by significance in the following order: heatassisted extraction>ultrasound bath and ultrasound probe>maceration. Relatively high
solubility of spray-dried extracts has been found in the following order: maceration>ultrasound
bath and ultrasound probe>heat-assisted extraction. Spray-dried T. serpyllum extract prepared
using maceration represents a powder with satisfying physicochemical properties, as well as a
good source of substances with significant antioxidant activity, particularly in emulsions, which
enable its use in functional food and pharmaceutical products.
PB  - Faculty of Technology, Zvornik
C3  - VI International Congress: „Engineering, Environment and Materials in Processing Industry“
T1  - Spray-dried extracts of Thymus serpyllum
EP  - 200
SP  - 193
DO  - 10.7251/EEMEN1901193J
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Petrović, Predrag M. and Đorđević, Verica B. and Častvan, Ivona M. and Lević, Steva M. and Zdunić, Gordana M. and Bugarski, Branko M.",
year = "2019",
abstract = "In the present study, spray-dried Thymus serpyllum extracts obtained by using four extraction
techniques (maceration, heat-assisted extraction, extraction in ultrasound bath and extraction by
ultrasound probe) were characterized through determination of total polyphenols and total
flavonoids contents, antioxidant capacity, zeta potential, bulk density and solubility. Polyphenols
yield was statistically higher in the extract obtained by ultrasound probe, in comparison to the
extracts prepared using maceration, heat-assisted extraction and extraction in ultrasound bath.
On the other hand, extraction technique did not show statistically significant influence on the
flavonoids concentration. Furthermore, extraction procedures did not show significant effect on
antioxidant neutralization of DPPH radicals, while flavonoids yield had correlation with the
DPPH radical-scavenging capacity assay. However, the extracts obtained using maceration and
ultrasound waves (ultrasound bath) have shown significantly better antioxidant capacity in the
preservation of β-carotene, compared to the extracts prepared by using high temperature and
ultrasound probe. The extract obtained in maceration has shown statistically better stability
(higher absolute value of zeta potential), compared to the extracts prepared using high
temperature and ultrasound waves. According to the results of Pharmacopoeia test, bulk density
of spray-dried T. serpyllum extracts was ranked by significance in the following order: heatassisted extraction>ultrasound bath and ultrasound probe>maceration. Relatively high
solubility of spray-dried extracts has been found in the following order: maceration>ultrasound
bath and ultrasound probe>heat-assisted extraction. Spray-dried T. serpyllum extract prepared
using maceration represents a powder with satisfying physicochemical properties, as well as a
good source of substances with significant antioxidant activity, particularly in emulsions, which
enable its use in functional food and pharmaceutical products.",
publisher = "Faculty of Technology, Zvornik",
journal = "VI International Congress: „Engineering, Environment and Materials in Processing Industry“",
title = "Spray-dried extracts of Thymus serpyllum",
pages = "200-193",
doi = "10.7251/EEMEN1901193J"
}

Jovanović, A. A., Petrović, P. M., Đorđević, V. B., Častvan, I. M., Lević, S. M., Zdunić, G. M.,& Bugarski, B. M.. (2019). Spray-dried extracts of Thymus serpyllum. in VI International Congress: „Engineering, Environment and Materials in Processing Industry“
Faculty of Technology, Zvornik., 193-200.
https://doi.org/10.7251/EEMEN1901193J

Jovanović AA, Petrović PM, Đorđević VB, Častvan IM, Lević SM, Zdunić GM, Bugarski BM. Spray-dried extracts of Thymus serpyllum. in VI International Congress: „Engineering, Environment and Materials in Processing Industry“. 2019;:193-200.
doi:10.7251/EEMEN1901193J .

Jovanović, Aleksandra A., Petrović, Predrag M., Đorđević, Verica B., Častvan, Ivona M., Lević, Steva M., Zdunić, Gordana M., Bugarski, Branko M., "Spray-dried extracts of Thymus serpyllum" in VI International Congress: „Engineering, Environment and Materials in Processing Industry“ (2019):193-200,
https://doi.org/10.7251/EEMEN1901193J . .

TY  - JOUR
AU  - Krunić, Tanja
AU  - Obradović, Nataša
AU  - Rakin, Marica
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4220
AB  - The main object of study is application of whey protein concentrate (WPC) and whey protein hydrolysate (WPH) for probiotic encapsulation. The controlled enzymatic hydrolysis was applied to change technological properties of WPC what leads to obtained different carriers. Probiotic carriers (beads) were made by electrostatic extrusion. Bead properties (mechanical properties, FTIR fingerprint, cell release) and parameters of fermentation were examined. According to cell release, it can be concluded that WPH build less porous matrix with alginate than WPC. Beads with WPH contained more living cells and suffered more changes during fermentation than beads with WPC. Probiotic viability in simulated gastrointestinal conditions (SGIC) is the most critical parameter for probiotic encapsulation. Probiotic encapsulated in protein-alginate beads survived SGIC with more than 96% viable cells, compared to 37.43% for free culture. According to all examined parameters, it can be concluded that WPH builds more suitable carrier for probiotic culture than WPC.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Application of whey protein and whey protein hydrolysate as protein based carrier for probiotic starter culture
EP  - 82
SP  - 74
VL  - 293
DO  - 10.1016/j.foodchem.2019.04.062
ER  - 

@article{
author = "Krunić, Tanja and Obradović, Nataša and Rakin, Marica",
year = "2019",
abstract = "The main object of study is application of whey protein concentrate (WPC) and whey protein hydrolysate (WPH) for probiotic encapsulation. The controlled enzymatic hydrolysis was applied to change technological properties of WPC what leads to obtained different carriers. Probiotic carriers (beads) were made by electrostatic extrusion. Bead properties (mechanical properties, FTIR fingerprint, cell release) and parameters of fermentation were examined. According to cell release, it can be concluded that WPH build less porous matrix with alginate than WPC. Beads with WPH contained more living cells and suffered more changes during fermentation than beads with WPC. Probiotic viability in simulated gastrointestinal conditions (SGIC) is the most critical parameter for probiotic encapsulation. Probiotic encapsulated in protein-alginate beads survived SGIC with more than 96% viable cells, compared to 37.43% for free culture. According to all examined parameters, it can be concluded that WPH builds more suitable carrier for probiotic culture than WPC.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Application of whey protein and whey protein hydrolysate as protein based carrier for probiotic starter culture",
pages = "82-74",
volume = "293",
doi = "10.1016/j.foodchem.2019.04.062"
}

Krunić, T., Obradović, N.,& Rakin, M.. (2019). Application of whey protein and whey protein hydrolysate as protein based carrier for probiotic starter culture. in Food Chemistry
Elsevier Sci Ltd, Oxford., 293, 74-82.
https://doi.org/10.1016/j.foodchem.2019.04.062

Krunić T, Obradović N, Rakin M. Application of whey protein and whey protein hydrolysate as protein based carrier for probiotic starter culture. in Food Chemistry. 2019;293:74-82.
doi:10.1016/j.foodchem.2019.04.062 .

Krunić, Tanja, Obradović, Nataša, Rakin, Marica, "Application of whey protein and whey protein hydrolysate as protein based carrier for probiotic starter culture" in Food Chemistry, 293 (2019):74-82,
https://doi.org/10.1016/j.foodchem.2019.04.062 . .

TY  - THES
AU  - Obradović, Nataša
PY  - 2019
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7465
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:22298/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=16629769
UR  - http://nardus.mpn.gov.rs/handle/123456789/17184
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4687
AB  - Probiotic cultures are becoming more popular in human nutrition primarily because modern consumers tend to use food products that have a more positive impact on their health. The therapeutic effect of food with added live microorganisms (probiotics), especially milk products, is promoted by health professionals. In order to achieve the desired therapeutic effect of food product, probiotic bacteria have to enter the body in appropriate number and maintain viability during production and storage conditions. After consuming the product, it is important that an adequate number of cells survive conditions in the gastrointestinal tract. Preparation of a natural hydrogel carrier, which will enable the protection of the probiotic starter culture during production, storage and the gastrointestinal conditions was goal of this doctoral dissertation. Aim was full potential impact of functional food product on human health. Thesis analyzes two types of results: characterization of obtained carriers with probiotic starter culture using different encapsulation techniques and application of the carriers in preparation of functional food product. Encapsulation tehniques used: (i) extrusion (ii) by spray-drying and (iii) freeze drying (lyophilization). Production of fermented whey-based beverage was used for implementation of prepared carriers with probiotic cells...
AB  - Probiotske kulture su sve više zastupljene u ljudskoj ishrani pre svega zbog težnje savremenih potrošača da konzumiraju kvalitetne prehrambene proizvodе koji imaju i pozitivan uticaj na ljudsko zdravlje. Terapeutski efekat hrane sa dodatim živim mikroorganizmima (probioticima) posebno na primerima mlečnih proizvoda se sve više promoviše od strane zdravstvenih radnika. Probiotske bakterije, da bi ostvarile željeni terapeutski efekat, neophodno je da se u organizam unesu u odgovarajućem broju i da se održi njihova vijabilnost tokom proizvodnog procesa i skladištenja proizvoda. Isto tako, posle konzumiranja proizvoda, potrebno je da adekvatan broj ćelija preživi uslove u gastro-intestinalnom traktu. Stoga je cilj ove doktorske disertacije bio priprema nosača na bazi prirodnih hidrogelova, koji će omogućiti zaštitu probiotske starter kulture tokom proizvodnje, skladištenja proizvoda i gastrointestinalnih uslova. Osim karakterizacije dobijenih nosača sa kulturom tehnikama inkapsulacije: (i) ekstruzijom (ii) sprej-sušenjem i (iii) sušenjem smrzavanjem (liofilizacijom), analizirana je i primena nosača u proizvodnji fermentisanih napitaka na bazi surutke...
PB  - Univerzitet u Beogradu, Tehnološko-metalurški fakultet
T1  - Characterization and application of natural hydrogels for encapsulation of probiotic starter culture
T1  - Karakterizacija i primena prirodnih hidrogelova za inkapsulaciju probiotske starter kulture
UR  - https://hdl.handle.net/21.15107/rcub_nardus_17184
ER  - 

@phdthesis{
author = "Obradović, Nataša",
year = "2019",
abstract = "Probiotic cultures are becoming more popular in human nutrition primarily because modern consumers tend to use food products that have a more positive impact on their health. The therapeutic effect of food with added live microorganisms (probiotics), especially milk products, is promoted by health professionals. In order to achieve the desired therapeutic effect of food product, probiotic bacteria have to enter the body in appropriate number and maintain viability during production and storage conditions. After consuming the product, it is important that an adequate number of cells survive conditions in the gastrointestinal tract. Preparation of a natural hydrogel carrier, which will enable the protection of the probiotic starter culture during production, storage and the gastrointestinal conditions was goal of this doctoral dissertation. Aim was full potential impact of functional food product on human health. Thesis analyzes two types of results: characterization of obtained carriers with probiotic starter culture using different encapsulation techniques and application of the carriers in preparation of functional food product. Encapsulation tehniques used: (i) extrusion (ii) by spray-drying and (iii) freeze drying (lyophilization). Production of fermented whey-based beverage was used for implementation of prepared carriers with probiotic cells..., Probiotske kulture su sve više zastupljene u ljudskoj ishrani pre svega zbog težnje savremenih potrošača da konzumiraju kvalitetne prehrambene proizvodе koji imaju i pozitivan uticaj na ljudsko zdravlje. Terapeutski efekat hrane sa dodatim živim mikroorganizmima (probioticima) posebno na primerima mlečnih proizvoda se sve više promoviše od strane zdravstvenih radnika. Probiotske bakterije, da bi ostvarile željeni terapeutski efekat, neophodno je da se u organizam unesu u odgovarajućem broju i da se održi njihova vijabilnost tokom proizvodnog procesa i skladištenja proizvoda. Isto tako, posle konzumiranja proizvoda, potrebno je da adekvatan broj ćelija preživi uslove u gastro-intestinalnom traktu. Stoga je cilj ove doktorske disertacije bio priprema nosača na bazi prirodnih hidrogelova, koji će omogućiti zaštitu probiotske starter kulture tokom proizvodnje, skladištenja proizvoda i gastrointestinalnih uslova. Osim karakterizacije dobijenih nosača sa kulturom tehnikama inkapsulacije: (i) ekstruzijom (ii) sprej-sušenjem i (iii) sušenjem smrzavanjem (liofilizacijom), analizirana je i primena nosača u proizvodnji fermentisanih napitaka na bazi surutke...",
publisher = "Univerzitet u Beogradu, Tehnološko-metalurški fakultet",
title = "Characterization and application of natural hydrogels for encapsulation of probiotic starter culture, Karakterizacija i primena prirodnih hidrogelova za inkapsulaciju probiotske starter kulture",
url = "https://hdl.handle.net/21.15107/rcub_nardus_17184"
}

Obradović, N.. (2019). Characterization and application of natural hydrogels for encapsulation of probiotic starter culture. 
Univerzitet u Beogradu, Tehnološko-metalurški fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_17184

Obradović N. Characterization and application of natural hydrogels for encapsulation of probiotic starter culture. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_17184 .

Obradović, Nataša, "Characterization and application of natural hydrogels for encapsulation of probiotic starter culture" (2019),
https://hdl.handle.net/21.15107/rcub_nardus_17184 .

TY  - JOUR
AU  - Jovanović, Aleksandra A.
AU  - Balanč, Bojana D.
AU  - Ota, Ajda
AU  - Đorđević, Verica B.
AU  - Šavikin, Katarina P.
AU  - Nedović, Viktor A.
AU  - Poklar Ulrih, Nataša
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5942
AB  - The aim of the present study was the examination of the impact of β-sitosterol and gentisic acid on the characteristics of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine(DPPC) liposomal particles: (a) bilayer permeability (fluorescence spectroscopy),(b) particle size, polydispersity index (PDI) and zeta potential (photon correlation spectroscopy) and (c) thermal properties (differential scanning calorimetry).β-sitosterol induced the increase of liposomal bilayer rigidity, due to rearranging of the phospholipid chains, while gentisic acid enhanced the membrane fluidity, due to the reduced orderliness and the increase of phospholipid dynamics. The inclusion of β-sitosterol in liposomes caused a significant increase in particle diameter and PDI, while the encapsulation of gentisic acid did not have influence on particle size distribution. Apart from that, the presence ofβ-sitosterol resulted in the significant zeta potential increase, and thus a better stability of liposomal spheres(in the absence and in the presence of gentisic acid). β-sitosterol decreased main transition temperature (Tm) and phase transition enthalpy (∆H), and caused the disappearance of the pre-transition peak as well, whereas the presence of gentisic acid produced a slight decrease in Tm and increase of ∆H. Therefore, gentisic acid had more favourable, stabilizing interactions with phospholipids thanβ-sitosterol. Thus, it can be concluded that β-sitosterol is located in the bilayer interior between phospholipids acyl chains, and gentisic acid is incorporated near the outer leaflet of the phospholipid membrane, next to the polar head groups.β-sitosterol and gentisic acid loaded DPPC liposomal particles have a potential to be used in food and pharmaceutical products, due to the important individual and possible synergistic beneficial health properties ofβ-sitosterol and gentisic acid.
PB  - University of East Sarajevo, Faculty of Technology
T2  - Journal of Engineering & Processing Management
T1  - β‐sitosterol and gentisic acid loaded1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphocholine liposomal particles
EP  - 88
IS  - 2
SP  - 81
VL  - 11
DO  - 10.7251/JEPM1902081A
ER  - 

@article{
author = "Jovanović, Aleksandra A. and Balanč, Bojana D. and Ota, Ajda and Đorđević, Verica B. and Šavikin, Katarina P. and Nedović, Viktor A. and Poklar Ulrih, Nataša",
year = "2019",
abstract = "The aim of the present study was the examination of the impact of β-sitosterol and gentisic acid on the characteristics of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine(DPPC) liposomal particles: (a) bilayer permeability (fluorescence spectroscopy),(b) particle size, polydispersity index (PDI) and zeta potential (photon correlation spectroscopy) and (c) thermal properties (differential scanning calorimetry).β-sitosterol induced the increase of liposomal bilayer rigidity, due to rearranging of the phospholipid chains, while gentisic acid enhanced the membrane fluidity, due to the reduced orderliness and the increase of phospholipid dynamics. The inclusion of β-sitosterol in liposomes caused a significant increase in particle diameter and PDI, while the encapsulation of gentisic acid did not have influence on particle size distribution. Apart from that, the presence ofβ-sitosterol resulted in the significant zeta potential increase, and thus a better stability of liposomal spheres(in the absence and in the presence of gentisic acid). β-sitosterol decreased main transition temperature (Tm) and phase transition enthalpy (∆H), and caused the disappearance of the pre-transition peak as well, whereas the presence of gentisic acid produced a slight decrease in Tm and increase of ∆H. Therefore, gentisic acid had more favourable, stabilizing interactions with phospholipids thanβ-sitosterol. Thus, it can be concluded that β-sitosterol is located in the bilayer interior between phospholipids acyl chains, and gentisic acid is incorporated near the outer leaflet of the phospholipid membrane, next to the polar head groups.β-sitosterol and gentisic acid loaded DPPC liposomal particles have a potential to be used in food and pharmaceutical products, due to the important individual and possible synergistic beneficial health properties ofβ-sitosterol and gentisic acid.",
publisher = "University of East Sarajevo, Faculty of Technology",
journal = "Journal of Engineering & Processing Management",
title = "β‐sitosterol and gentisic acid loaded1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphocholine liposomal particles",
pages = "88-81",
number = "2",
volume = "11",
doi = "10.7251/JEPM1902081A"
}

Jovanović, A. A., Balanč, B. D., Ota, A., Đorđević, V. B., Šavikin, K. P., Nedović, V. A.,& Poklar Ulrih, N.. (2019). β‐sitosterol and gentisic acid loaded1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphocholine liposomal particles. in Journal of Engineering & Processing Management
University of East Sarajevo, Faculty of Technology., 11(2), 81-88.
https://doi.org/10.7251/JEPM1902081A

Jovanović AA, Balanč BD, Ota A, Đorđević VB, Šavikin KP, Nedović VA, Poklar Ulrih N. β‐sitosterol and gentisic acid loaded1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphocholine liposomal particles. in Journal of Engineering & Processing Management. 2019;11(2):81-88.
doi:10.7251/JEPM1902081A .

Jovanović, Aleksandra A., Balanč, Bojana D., Ota, Ajda, Đorđević, Verica B., Šavikin, Katarina P., Nedović, Viktor A., Poklar Ulrih, Nataša, "β‐sitosterol and gentisic acid loaded1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphocholine liposomal particles" in Journal of Engineering & Processing Management, 11, no. 2 (2019):81-88,
https://doi.org/10.7251/JEPM1902081A . .