TY  - CONF
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Petrov Ivanković, Anja
AU  - Ćorović, Marija
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6962
AB  - Fructo-oligosaccharides (FOS) are linear bioactive molecules consisting of one
terminal glucose and other fructose units and belong to the group of prebiotics with
scientifically proven benefits for human health. Physiological active compounds like
trisaccharides (FOS3), tetrasaccharides (FOS4) and pentasaccharides (FOS5) can be
produced from sucrose using enzyme-fructosyltransferase. The reaction mixture obtained in
this way contains, in addition to the desired prebiotics, glucose, fructose and an unreacted
amount of sucrose. Direct incorporation in products of food and pharmaceutical origin is
limited due to the presence of unwanted carbohydrates (mono- and disaccharide), and to
increase the functional value of the mixture, the attention was focused on their removal. In
this regard, membrane separation techniques present a very suitable solution for obtaining a
product with a higher FOS proportion. This study aimed to determine the optimal conditions
for purification process of produced FOS mixture using nanofiltration membrane modules.
Therefore, the influence of membranes with different MWCO (300-500Da and 600-800Da),
temperature (20-45°C) and carbohydrate concentration in feed solution (1-5%) at a constant
flow rate of 22 mL/min was investigated. From the initial mixture containing 60.2% FOS,
29.7% monosaccharides and 10.1% sucrose, after purification process under determined
optimal conditions (membrane 300-500Da, temperature 35°C and carbohydrate concentration
3%), the product with 88.8% FOS, 10.0% sucrose and 1.2% monosaccharides in total
carbohydrates was obtained. Purification yield was greater than 95% and the rejection
coefficients for glucose, fructose, sucrose, FOS3, FOS4 and FOS5 were 0.17, 0.20, 0.95,
0.99, 1.00 and 1.00, respectively. Based on the calculated purification factor, it can be
concluded that the initial mixture is refined 1.47 times. This result suggests a significant
increase in the purity of the final mixture, which can be used as a sugar substitute or addition
to numerous products that would be suitable for all consumers, including those suffering from
diabetes.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES
SP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6962
ER  - 

@conference{
author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Petrov Ivanković, Anja and Ćorović, Marija and Bezbradica, Dejan",
year = "2023",
abstract = "Fructo-oligosaccharides (FOS) are linear bioactive molecules consisting of one
terminal glucose and other fructose units and belong to the group of prebiotics with
scientifically proven benefits for human health. Physiological active compounds like
trisaccharides (FOS3), tetrasaccharides (FOS4) and pentasaccharides (FOS5) can be
produced from sucrose using enzyme-fructosyltransferase. The reaction mixture obtained in
this way contains, in addition to the desired prebiotics, glucose, fructose and an unreacted
amount of sucrose. Direct incorporation in products of food and pharmaceutical origin is
limited due to the presence of unwanted carbohydrates (mono- and disaccharide), and to
increase the functional value of the mixture, the attention was focused on their removal. In
this regard, membrane separation techniques present a very suitable solution for obtaining a
product with a higher FOS proportion. This study aimed to determine the optimal conditions
for purification process of produced FOS mixture using nanofiltration membrane modules.
Therefore, the influence of membranes with different MWCO (300-500Da and 600-800Da),
temperature (20-45°C) and carbohydrate concentration in feed solution (1-5%) at a constant
flow rate of 22 mL/min was investigated. From the initial mixture containing 60.2% FOS,
29.7% monosaccharides and 10.1% sucrose, after purification process under determined
optimal conditions (membrane 300-500Da, temperature 35°C and carbohydrate concentration
3%), the product with 88.8% FOS, 10.0% sucrose and 1.2% monosaccharides in total
carbohydrates was obtained. Purification yield was greater than 95% and the rejection
coefficients for glucose, fructose, sucrose, FOS3, FOS4 and FOS5 were 0.17, 0.20, 0.95,
0.99, 1.00 and 1.00, respectively. Based on the calculated purification factor, it can be
concluded that the initial mixture is refined 1.47 times. This result suggests a significant
increase in the purity of the final mixture, which can be used as a sugar substitute or addition
to numerous products that would be suitable for all consumers, including those suffering from
diabetes.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES",
pages = "86",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6962"
}

Veljković, M., Simović, M., Banjanac, K., Petrov Ivanković, A., Ćorović, M.,& Bezbradica, D.. (2023). NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 86.
https://hdl.handle.net/21.15107/rcub_technorep_6962

Veljković M, Simović M, Banjanac K, Petrov Ivanković A, Ćorović M, Bezbradica D. NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:86.
https://hdl.handle.net/21.15107/rcub_technorep_6962 .

Veljković, Milica, Simović, Milica, Banjanac, Katarina, Petrov Ivanković, Anja, Ćorović, Marija, Bezbradica, Dejan, "NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):86,
https://hdl.handle.net/21.15107/rcub_technorep_6962 .

TY  - CONF
AU  - Ortega-Nieto, Clara
AU  - Losada-Gracia, Noelia
AU  - Vukoičić, Ana
AU  - Milivojević, Ana
AU  - Bezbradica, Dejan
AU  - Palomo, Jose M.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6959
AB  - In recent years, the synthesis of novel artificial metalloenzymes by the combination of
metal or complex organometallic systems and enzymes is rapidly growing field of research in
catalytic applications. In this study, new artificial metalloenzymes have been designed and
developed based on the combination of an enzyme and different metal salts. For this purpose,
novel synthesis method has been used, where the enzyme, Lipase B from Candida antarctica
(CALB), acts as an inducer of metal nanoparticles of copper and silver. Obtained biohybrids
show improved stability properties compared to natural enzymes. Moreover, the synergetic
effect between copper and silver has been evaluated, looking for an improvement in the
catalytic efficiency of an only copper-metalloenzyme previously developed.
The copper-silver-CALB metalloenzymes have been characterized by X-Ray
diffraction analysis, mass spectrometry, and SEM and TEM as microscopies techniques. After
that, their catalytic efficiency was tested in different reactions, in particular in the phloridzin
oligomerization as laccase-like activity.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6959
ER  - 

@conference{
author = "Ortega-Nieto, Clara and Losada-Gracia, Noelia and Vukoičić, Ana and Milivojević, Ana and Bezbradica, Dejan and Palomo, Jose M.",
year = "2023",
abstract = "In recent years, the synthesis of novel artificial metalloenzymes by the combination of
metal or complex organometallic systems and enzymes is rapidly growing field of research in
catalytic applications. In this study, new artificial metalloenzymes have been designed and
developed based on the combination of an enzyme and different metal salts. For this purpose,
novel synthesis method has been used, where the enzyme, Lipase B from Candida antarctica
(CALB), acts as an inducer of metal nanoparticles of copper and silver. Obtained biohybrids
show improved stability properties compared to natural enzymes. Moreover, the synergetic
effect between copper and silver has been evaluated, looking for an improvement in the
catalytic efficiency of an only copper-metalloenzyme previously developed.
The copper-silver-CALB metalloenzymes have been characterized by X-Ray
diffraction analysis, mass spectrometry, and SEM and TEM as microscopies techniques. After
that, their catalytic efficiency was tested in different reactions, in particular in the phloridzin
oligomerization as laccase-like activity.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6959"
}

Ortega-Nieto, C., Losada-Gracia, N., Vukoičić, A., Milivojević, A., Bezbradica, D.,& Palomo, J. M.. (2023). NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 39.
https://hdl.handle.net/21.15107/rcub_technorep_6959

Ortega-Nieto C, Losada-Gracia N, Vukoičić A, Milivojević A, Bezbradica D, Palomo JM. NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:39.
https://hdl.handle.net/21.15107/rcub_technorep_6959 .

Ortega-Nieto, Clara, Losada-Gracia, Noelia, Vukoičić, Ana, Milivojević, Ana, Bezbradica, Dejan, Palomo, Jose M., "NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):39,
https://hdl.handle.net/21.15107/rcub_technorep_6959 .