TY  - CONF
AU  - Volić, Mina
AU  - Obradović, Nataša
AU  - Jovanović, Aleksandra
AU  - Bugarski, Branko
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6451
AB  - Cherry fruits are known for their wide range of nutritive and bioactive compounds, including
fibers, vitamins, and phytonutrients. Sweet cherry consumption has been associated with
several health benefits against human problems such as cardiovascular diseases, cancer,
diabetes, and Alzheimer’s disease. Particularly, cherries contain abundant phenolic compounds
with antioxidant, anti-inflammatory, and anticancer properties as they exert a protective role
against oxidative stress and free radical damages. However, there is not a specific method to
fully extract all phenolics and other compounds from sweet cherries. Thus, this study aimed to
optimize the extraction of cherries through different extraction procedures (maceration and
ultrasound-assisted extraction) using different solvents (50% EtOH with and without HCl, and
Tris buffer). The resulting cherry fruit extracts were investigated regarding their i) total phenol
content - according to the Folin-Ciocalteu colorimetric method, ii) total protein content -
evaluated using the Bradford protein assay, and iii) antioxidant activity – characterized by
ABTS, DPPH, and FRAP assays. The ultrasound-assisted extraction using 50% EtOH and HCl
(pH=2.3) demonstrated the best results for total phenol content (7.51 mg/L), DPPH inhibition
(23.7%), and FRAP reducing power (24.5 mmol/g). On the contrary, the highest ABTS
scavenging activity was noticed for maceration obtained extract with 50% EtOH and HCl (7.23
mg/mL). Finally, the best protein yield was obtained for cherries when extraction was
performed in 0.1M Tris buffer at pH=11.2 (0.23 mg/mL). The optimization methodology in this
work is used as a screening test for producing bioactive compounds-rich extracts for the food
industry
PB  - University of Novi Sad, Faculty of Technology Novi Sad
C3  - Book of Abstracts of the 2nd International Conference on Advanced Production and Processing
T1  - Optimization of Sweet Cherry Extraction: Evaluation of Total Phenol Content, Protein Content, and Antioxidant Activity of Extracts
SP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6451
ER  - 

@conference{
author = "Volić, Mina and Obradović, Nataša and Jovanović, Aleksandra and Bugarski, Branko",
year = "2022",
abstract = "Cherry fruits are known for their wide range of nutritive and bioactive compounds, including
fibers, vitamins, and phytonutrients. Sweet cherry consumption has been associated with
several health benefits against human problems such as cardiovascular diseases, cancer,
diabetes, and Alzheimer’s disease. Particularly, cherries contain abundant phenolic compounds
with antioxidant, anti-inflammatory, and anticancer properties as they exert a protective role
against oxidative stress and free radical damages. However, there is not a specific method to
fully extract all phenolics and other compounds from sweet cherries. Thus, this study aimed to
optimize the extraction of cherries through different extraction procedures (maceration and
ultrasound-assisted extraction) using different solvents (50% EtOH with and without HCl, and
Tris buffer). The resulting cherry fruit extracts were investigated regarding their i) total phenol
content - according to the Folin-Ciocalteu colorimetric method, ii) total protein content -
evaluated using the Bradford protein assay, and iii) antioxidant activity – characterized by
ABTS, DPPH, and FRAP assays. The ultrasound-assisted extraction using 50% EtOH and HCl
(pH=2.3) demonstrated the best results for total phenol content (7.51 mg/L), DPPH inhibition
(23.7%), and FRAP reducing power (24.5 mmol/g). On the contrary, the highest ABTS
scavenging activity was noticed for maceration obtained extract with 50% EtOH and HCl (7.23
mg/mL). Finally, the best protein yield was obtained for cherries when extraction was
performed in 0.1M Tris buffer at pH=11.2 (0.23 mg/mL). The optimization methodology in this
work is used as a screening test for producing bioactive compounds-rich extracts for the food
industry",
publisher = "University of Novi Sad, Faculty of Technology Novi Sad",
journal = "Book of Abstracts of the 2nd International Conference on Advanced Production and Processing",
title = "Optimization of Sweet Cherry Extraction: Evaluation of Total Phenol Content, Protein Content, and Antioxidant Activity of Extracts",
pages = "180",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6451"
}

Volić, M., Obradović, N., Jovanović, A.,& Bugarski, B.. (2022). Optimization of Sweet Cherry Extraction: Evaluation of Total Phenol Content, Protein Content, and Antioxidant Activity of Extracts. in Book of Abstracts of the 2nd International Conference on Advanced Production and Processing
University of Novi Sad, Faculty of Technology Novi Sad., 180.
https://hdl.handle.net/21.15107/rcub_technorep_6451

Volić M, Obradović N, Jovanović A, Bugarski B. Optimization of Sweet Cherry Extraction: Evaluation of Total Phenol Content, Protein Content, and Antioxidant Activity of Extracts. in Book of Abstracts of the 2nd International Conference on Advanced Production and Processing. 2022;:180.
https://hdl.handle.net/21.15107/rcub_technorep_6451 .

Volić, Mina, Obradović, Nataša, Jovanović, Aleksandra, Bugarski, Branko, "Optimization of Sweet Cherry Extraction: Evaluation of Total Phenol Content, Protein Content, and Antioxidant Activity of Extracts" in Book of Abstracts of the 2nd International Conference on Advanced Production and Processing (2022):180,
https://hdl.handle.net/21.15107/rcub_technorep_6451 .