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Use of Candida rugosa lipase immobilized on sepabeads for the amyl caprylate synthesis: Batch and fluidized bed reactor study
dc.creator | Šaponjić, Svetlana V. | |
dc.creator | Knežević-Jugović, Zorica | |
dc.creator | Bezbradica, Dejan | |
dc.creator | Žuža, Milena | |
dc.creator | Saied, Omar Ali | |
dc.creator | Bošković-Vragolović, Nevenka | |
dc.creator | Mijin, Dušan | |
dc.date.accessioned | 2021-03-10T11:22:29Z | |
dc.date.available | 2021-03-10T11:22:29Z | |
dc.date.issued | 2010 | |
dc.identifier.issn | 0717-3458 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1691 | |
dc.description.abstract | Lipase from Candida rugosa was covalently immobilized on Sepabeads EC-EP for application for amyl caprylate synthesis in an organic solvent system. Several solvents were tested in terms of biocatalyst stability and the best result was obtained with isooctane. The lipase-catalyzed esterification in the selected system was performed in batch and fluidized bed reactor systems. The influence of several important reaction parameters including temperature, initial water content, enzyme loading, acid/alcohol molar ratio, and time of addition of molecular sieves is carefully analyzed by means of an experimental design. Almost complete conversion ( gt 99%) of the substrate to ester could be performed in a batch reactor system, using lipase loading as low as 37 mg g(-1) dry support and in a relatively short time (24 hrs) at 37 degrees C, when high initial substrate molar ratio of 2.2 is used. Kinetics in a fluidized bed reactor system seems to still have a slightly better profile than in the batch system (90.2% yields after 14 hrs). The fluidized bed reactor operated for up 70 hrs almost with no loss in productivity, implying that the proposed process and the immobilized system could provide a promising approach for the amyl caprylate synthesis at the industrial scale. | en |
dc.publisher | University of Catolica De Valparaiso, Valparaiso | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/20064/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Electronic Journal of Biotechnology | |
dc.subject | bioreactors | en |
dc.subject | covalent immobilization | en |
dc.subject | ester | en |
dc.subject | non-aqueous system | en |
dc.subject | optimization technique | en |
dc.title | Use of Candida rugosa lipase immobilized on sepabeads for the amyl caprylate synthesis: Batch and fluidized bed reactor study | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dc.citation.issue | 6 | |
dc.citation.other | 13(6): - | |
dc.citation.rank | M23 | |
dc.citation.volume | 13 | |
dc.identifier.doi | 10.2225/vol13-issue6-fulltext-8 | |
dc.identifier.fulltext | http://TechnoRep.tmf.bg.ac.rs/bitstream/id/296/1688.pdf | |
dc.identifier.scopus | 2-s2.0-79960870106 | |
dc.identifier.wos | 000288177100008 | |
dc.type.version | publishedVersion |