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A highly efficient diastereoselective synthesis of alpha-isosalicin by maltase from Saccharomyces cerevisiae
dc.creator | Veličković, Dušan | |
dc.creator | Dimitrijević, Aleksandra | |
dc.creator | Bihelović, Filip | |
dc.creator | Bezbradica, Dejan | |
dc.creator | Jankov, Ratko | |
dc.creator | Milosavić, Nenad | |
dc.date.accessioned | 2021-03-10T11:35:22Z | |
dc.date.available | 2021-03-10T11:35:22Z | |
dc.date.issued | 2011 | |
dc.identifier.issn | 1359-5113 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1891 | |
dc.description.abstract | In this report, alpha-isosalicin, a potent anticoagulant and skin whitening agent, was synthesized by a highly efficient chemoselective and diastereoselective reaction, catalyzed by maltase from bakers' yeast (Saccharomyces cerevisiae). The highest yield of this one-step transglucosylation reaction was achieved with 50 mM of salicyl alcohol as a glucose acceptor. The key reaction factors were optimized using response surface methodology (RSM) with an enzyme concentration of 10 U/mL. The optimum temperature of the reaction was determined as 36.5 degrees C, the optimal maltose concentration was 40% (w/v), the optimal pH was 6.5, and the optimal reaction time was 16 h. Under these conditions 75% of alpha-isosalicin was obtained, with a yield of 10 g/L, and no by product formation was observed. | en |
dc.publisher | Elsevier Sci Ltd, Oxford | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS// | |
dc.rights | restrictedAccess | |
dc.source | Process Biochemistry | |
dc.subject | alpha-Isosalicin | en |
dc.subject | Glucosidase | en |
dc.subject | Salicyl alcohol | en |
dc.subject | Transglucosylation | en |
dc.subject | Bakers' yeast | en |
dc.title | A highly efficient diastereoselective synthesis of alpha-isosalicin by maltase from Saccharomyces cerevisiae | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 1702 | |
dc.citation.issue | 8 | |
dc.citation.other | 46(8): 1698-1702 | |
dc.citation.rank | M21 | |
dc.citation.spage | 1698 | |
dc.citation.volume | 46 | |
dc.identifier.doi | 10.1016/j.procbio.2011.05.007 | |
dc.identifier.scopus | 2-s2.0-79959922337 | |
dc.identifier.wos | 000293438300025 | |
dc.type.version | publishedVersion |