Impact of ultrasound on egg white proteins as a pretreatment for functional hydrolysates production
Само за регистроване кориснике
2014
Аутори
Stefanović, AndreaJovanović, Jelena
Grbavčić, Sanja
Šekuljica, Nataša
Manojlović, Verica
Bugarski, Branko
Knežević-Jugović, Zorica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The objective of this study was to investigate the effects of different ultrasound pretreatment on enzymatic hydrolysis of egg white proteins (EWPs) by Alcalase as well as evaluating some functional and antioxidant properties of hydrolysates obtained by various proteases treatment and ultrasound technology. The effects of chosen ultrasound pretreatment parameters including frequency of ultrasonic waves (35 and 40 kHz), temperature (25 and 55 A degrees C), time of pretreatment (15-60 min) and pH of egg white solution (7.00-10.00) were examined. It appeared that controlled ultrasound treatment can improved the hydrolysis process compared with untreated samples, but optimization of the power and length of sonication was important. The optimal ultrasound pretreatment at calorimetric power of 21.3 W and frequency of 40 kHz for 15 min at 25 A degrees C and with naturally basic egg white (pH 9.25) resulted in increased initial rate and equilibrium degree of Alcalase hydrolysis by about 139.8 ...and 13.86 % compared with the control, respectively. EWP hydrolysates with a parts per thousand 27.0 % degree of hydrolysis obtained with heat pretreatment and ultrasound pretreatments under optimal conditions were further separated by sequential ultrafiltration into 4 hydrolysate fractions ( lt 1, 1-10, 10-30 and gt 30 kDa) which were investigated for protein content, peptide yield and antioxidant activity. The hydrolysis after heat pretreatment generated more peptides lt 1 kDa (19.04 +/- A 1.02 %) than ultrasound pretreatment did (11.90 +/- A 0.53 %), whereas the proportion of peptides lt 10 kDa were higher in the second case (28.80 +/- A 0.07 vs. 20.46 +/- A 0.39 %). The fraction obtained by the ultrasound pretreatment containing peptides with a molecular weight between 1 and 10 kDa demonstrated the strongest ABTS radical scavenging efficacy among the fractions (97.54 +/- A 0.30) with IC50 value of 4.31 mg/mL. Compared with single-enzyme processes, the two-stage enzymatic processes did not significantly improve both antioxidant and functional hydrolysates' properties.
Кључне речи:
Ultrasound pretreatment / Proteases / Egg white hydrolysates / Antioxidant activity / Functional propertiesИзвор:
European Food Research and Technology, 2014, 239, 6, 979-993Издавач:
- Springer, New York
Финансирање / пројекти:
- EUREKA E!6750
- Ministry of Education, Science and Technological Development of the Republic of Serbia
DOI: 10.1007/s00217-014-2295-8
ISSN: 1438-2377
WoS: 000345071400009
Scopus: 2-s2.0-84911986451
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Stefanović, Andrea AU - Jovanović, Jelena AU - Grbavčić, Sanja AU - Šekuljica, Nataša AU - Manojlović, Verica AU - Bugarski, Branko AU - Knežević-Jugović, Zorica PY - 2014 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2701 AB - The objective of this study was to investigate the effects of different ultrasound pretreatment on enzymatic hydrolysis of egg white proteins (EWPs) by Alcalase as well as evaluating some functional and antioxidant properties of hydrolysates obtained by various proteases treatment and ultrasound technology. The effects of chosen ultrasound pretreatment parameters including frequency of ultrasonic waves (35 and 40 kHz), temperature (25 and 55 A degrees C), time of pretreatment (15-60 min) and pH of egg white solution (7.00-10.00) were examined. It appeared that controlled ultrasound treatment can improved the hydrolysis process compared with untreated samples, but optimization of the power and length of sonication was important. The optimal ultrasound pretreatment at calorimetric power of 21.3 W and frequency of 40 kHz for 15 min at 25 A degrees C and with naturally basic egg white (pH 9.25) resulted in increased initial rate and equilibrium degree of Alcalase hydrolysis by about 139.8 and 13.86 % compared with the control, respectively. EWP hydrolysates with a parts per thousand 27.0 % degree of hydrolysis obtained with heat pretreatment and ultrasound pretreatments under optimal conditions were further separated by sequential ultrafiltration into 4 hydrolysate fractions ( lt 1, 1-10, 10-30 and gt 30 kDa) which were investigated for protein content, peptide yield and antioxidant activity. The hydrolysis after heat pretreatment generated more peptides lt 1 kDa (19.04 +/- A 1.02 %) than ultrasound pretreatment did (11.90 +/- A 0.53 %), whereas the proportion of peptides lt 10 kDa were higher in the second case (28.80 +/- A 0.07 vs. 20.46 +/- A 0.39 %). The fraction obtained by the ultrasound pretreatment containing peptides with a molecular weight between 1 and 10 kDa demonstrated the strongest ABTS radical scavenging efficacy among the fractions (97.54 +/- A 0.30) with IC50 value of 4.31 mg/mL. Compared with single-enzyme processes, the two-stage enzymatic processes did not significantly improve both antioxidant and functional hydrolysates' properties. PB - Springer, New York T2 - European Food Research and Technology T1 - Impact of ultrasound on egg white proteins as a pretreatment for functional hydrolysates production EP - 993 IS - 6 SP - 979 VL - 239 DO - 10.1007/s00217-014-2295-8 ER -
@article{ author = "Stefanović, Andrea and Jovanović, Jelena and Grbavčić, Sanja and Šekuljica, Nataša and Manojlović, Verica and Bugarski, Branko and Knežević-Jugović, Zorica", year = "2014", abstract = "The objective of this study was to investigate the effects of different ultrasound pretreatment on enzymatic hydrolysis of egg white proteins (EWPs) by Alcalase as well as evaluating some functional and antioxidant properties of hydrolysates obtained by various proteases treatment and ultrasound technology. The effects of chosen ultrasound pretreatment parameters including frequency of ultrasonic waves (35 and 40 kHz), temperature (25 and 55 A degrees C), time of pretreatment (15-60 min) and pH of egg white solution (7.00-10.00) were examined. It appeared that controlled ultrasound treatment can improved the hydrolysis process compared with untreated samples, but optimization of the power and length of sonication was important. The optimal ultrasound pretreatment at calorimetric power of 21.3 W and frequency of 40 kHz for 15 min at 25 A degrees C and with naturally basic egg white (pH 9.25) resulted in increased initial rate and equilibrium degree of Alcalase hydrolysis by about 139.8 and 13.86 % compared with the control, respectively. EWP hydrolysates with a parts per thousand 27.0 % degree of hydrolysis obtained with heat pretreatment and ultrasound pretreatments under optimal conditions were further separated by sequential ultrafiltration into 4 hydrolysate fractions ( lt 1, 1-10, 10-30 and gt 30 kDa) which were investigated for protein content, peptide yield and antioxidant activity. The hydrolysis after heat pretreatment generated more peptides lt 1 kDa (19.04 +/- A 1.02 %) than ultrasound pretreatment did (11.90 +/- A 0.53 %), whereas the proportion of peptides lt 10 kDa were higher in the second case (28.80 +/- A 0.07 vs. 20.46 +/- A 0.39 %). The fraction obtained by the ultrasound pretreatment containing peptides with a molecular weight between 1 and 10 kDa demonstrated the strongest ABTS radical scavenging efficacy among the fractions (97.54 +/- A 0.30) with IC50 value of 4.31 mg/mL. Compared with single-enzyme processes, the two-stage enzymatic processes did not significantly improve both antioxidant and functional hydrolysates' properties.", publisher = "Springer, New York", journal = "European Food Research and Technology", title = "Impact of ultrasound on egg white proteins as a pretreatment for functional hydrolysates production", pages = "993-979", number = "6", volume = "239", doi = "10.1007/s00217-014-2295-8" }
Stefanović, A., Jovanović, J., Grbavčić, S., Šekuljica, N., Manojlović, V., Bugarski, B.,& Knežević-Jugović, Z.. (2014). Impact of ultrasound on egg white proteins as a pretreatment for functional hydrolysates production. in European Food Research and Technology Springer, New York., 239(6), 979-993. https://doi.org/10.1007/s00217-014-2295-8
Stefanović A, Jovanović J, Grbavčić S, Šekuljica N, Manojlović V, Bugarski B, Knežević-Jugović Z. Impact of ultrasound on egg white proteins as a pretreatment for functional hydrolysates production. in European Food Research and Technology. 2014;239(6):979-993. doi:10.1007/s00217-014-2295-8 .
Stefanović, Andrea, Jovanović, Jelena, Grbavčić, Sanja, Šekuljica, Nataša, Manojlović, Verica, Bugarski, Branko, Knežević-Jugović, Zorica, "Impact of ultrasound on egg white proteins as a pretreatment for functional hydrolysates production" in European Food Research and Technology, 239, no. 6 (2014):979-993, https://doi.org/10.1007/s00217-014-2295-8 . .