Surface functionalization of oxidized multi-walled carbon nanotubes: Candida rugosa lipase immobilization
2016
Аутори
Prlainović, NevenaBezbradica, Dejan
Rogan, Jelena
Uskoković, Petar
Mijin, Dušan
Marinković, Aleksandar
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This work examines two approaches for immobilization of lipase from Candida rugosa on oxidized multi-walled carbon nanotubes (o-MWCNTs). One method included the presence of activating agents to promote covalent bonding and the other the adsorption on o-MWCNTs to elucidate if non-specific bonding on the o-MWCNTs surface exists. The influence of the immobilization time and initial enzyme concentration on protein loading and the expressed lypolitic activity of the immobilized preparation were investigated. The results showed that the enzyme adsorbs on o-MWCNTs in a maximal amount of 37 mu g mg(-1) CNTs, while the attached amount was more than 2-times higher under covalent promoting conditions (80 mu g mg(-1) CNTs). Furthermore, similar trends were observed for the lypolitic activity, whereby preparations obtained under covalent promoting conditions had almost 3-times higher activity (560 IU g(-1) of immobilized enzyme). In addition, immobilization of the enzyme was confirmed by Fourier tr...ansformation infrared spectroscopy and thermogravimetric analysis.
Кључне речи:
Candida rugosa lipase / Enzyme immobilization / Carbon nanotubes / o-MWCNT / Carbodiimide chemistryИзвор:
Comptes Rendus Chimie, 2016, 19, 3, 363-370Издавач:
- Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
Финансирање / пројекти:
- Проучавање синтезе, структуре и активности органских једињења природног и синтетског порекла (RS-MESTD-Basic Research (BR or ON)-172013)
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Алергени, антитела, ензими и мали физиолошки значајни молекули: дизајн, структура, функција и значај (RS-MESTD-Basic Research (BR or ON)-172049)
DOI: 10.1016/j.crci.2015.10.008
ISSN: 1631-0748
WoS: 000385784900014
Scopus: 2-s2.0-84955495328
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Prlainović, Nevena AU - Bezbradica, Dejan AU - Rogan, Jelena AU - Uskoković, Petar AU - Mijin, Dušan AU - Marinković, Aleksandar PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3351 AB - This work examines two approaches for immobilization of lipase from Candida rugosa on oxidized multi-walled carbon nanotubes (o-MWCNTs). One method included the presence of activating agents to promote covalent bonding and the other the adsorption on o-MWCNTs to elucidate if non-specific bonding on the o-MWCNTs surface exists. The influence of the immobilization time and initial enzyme concentration on protein loading and the expressed lypolitic activity of the immobilized preparation were investigated. The results showed that the enzyme adsorbs on o-MWCNTs in a maximal amount of 37 mu g mg(-1) CNTs, while the attached amount was more than 2-times higher under covalent promoting conditions (80 mu g mg(-1) CNTs). Furthermore, similar trends were observed for the lypolitic activity, whereby preparations obtained under covalent promoting conditions had almost 3-times higher activity (560 IU g(-1) of immobilized enzyme). In addition, immobilization of the enzyme was confirmed by Fourier transformation infrared spectroscopy and thermogravimetric analysis. PB - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux T2 - Comptes Rendus Chimie T1 - Surface functionalization of oxidized multi-walled carbon nanotubes: Candida rugosa lipase immobilization EP - 370 IS - 3 SP - 363 VL - 19 DO - 10.1016/j.crci.2015.10.008 ER -
@article{ author = "Prlainović, Nevena and Bezbradica, Dejan and Rogan, Jelena and Uskoković, Petar and Mijin, Dušan and Marinković, Aleksandar", year = "2016", abstract = "This work examines two approaches for immobilization of lipase from Candida rugosa on oxidized multi-walled carbon nanotubes (o-MWCNTs). One method included the presence of activating agents to promote covalent bonding and the other the adsorption on o-MWCNTs to elucidate if non-specific bonding on the o-MWCNTs surface exists. The influence of the immobilization time and initial enzyme concentration on protein loading and the expressed lypolitic activity of the immobilized preparation were investigated. The results showed that the enzyme adsorbs on o-MWCNTs in a maximal amount of 37 mu g mg(-1) CNTs, while the attached amount was more than 2-times higher under covalent promoting conditions (80 mu g mg(-1) CNTs). Furthermore, similar trends were observed for the lypolitic activity, whereby preparations obtained under covalent promoting conditions had almost 3-times higher activity (560 IU g(-1) of immobilized enzyme). In addition, immobilization of the enzyme was confirmed by Fourier transformation infrared spectroscopy and thermogravimetric analysis.", publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux", journal = "Comptes Rendus Chimie", title = "Surface functionalization of oxidized multi-walled carbon nanotubes: Candida rugosa lipase immobilization", pages = "370-363", number = "3", volume = "19", doi = "10.1016/j.crci.2015.10.008" }
Prlainović, N., Bezbradica, D., Rogan, J., Uskoković, P., Mijin, D.,& Marinković, A.. (2016). Surface functionalization of oxidized multi-walled carbon nanotubes: Candida rugosa lipase immobilization. in Comptes Rendus Chimie Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 19(3), 363-370. https://doi.org/10.1016/j.crci.2015.10.008
Prlainović N, Bezbradica D, Rogan J, Uskoković P, Mijin D, Marinković A. Surface functionalization of oxidized multi-walled carbon nanotubes: Candida rugosa lipase immobilization. in Comptes Rendus Chimie. 2016;19(3):363-370. doi:10.1016/j.crci.2015.10.008 .
Prlainović, Nevena, Bezbradica, Dejan, Rogan, Jelena, Uskoković, Petar, Mijin, Dušan, Marinković, Aleksandar, "Surface functionalization of oxidized multi-walled carbon nanotubes: Candida rugosa lipase immobilization" in Comptes Rendus Chimie, 19, no. 3 (2016):363-370, https://doi.org/10.1016/j.crci.2015.10.008 . .