Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This study provides a detailed analysis of the lignocellulolytic activity of a new isolate Stereum gausapatum F28, a Serbian autochthonous fungi, on beechwood sawdust supplemented with cheap waste, sugar beet molasses still age. Advanced multiple response optimization techniques were applied to improve ligninolytic and reduce hydrolytic activity as a requirement for potential biorefinery use. The applied techniques were supposed to select cultivation conditions that would give manganese peroxidase and laccase activities above 0.84 and 0.12 U g(-1) substrate, respectively, and cellulase and xylanase activities below 1.12 and 1.4 U g(-1) substrate. The optimal cultivation conditions that met the set requirements included molasses stillage concentration of 10 %, substrate moisture content of 53 %, incubation temperature of 23.5 degrees C, and pH 5.2. The research showed that the addition of molasses stillage had a positive effect on enzyme production and that the optimal stillage concentr...ation differed depending on the enzyme type (for laccase it was lt 5 %, manganese peroxidase approximate to 12 %, cellulase approximate to 21 % and xylanase approximate to 16 %), which should be taken into consideration when optimizing the desired process..
Кључне речи:
response surface method / genetic algorithm / fungi / biomass / enzymesИзвор:
Journal of the Serbian Chemical Society, 2022Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Jovic, Jelena AU - Hao, Jian AU - Mojović, Ljiljana PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4975 AB - This study provides a detailed analysis of the lignocellulolytic activity of a new isolate Stereum gausapatum F28, a Serbian autochthonous fungi, on beechwood sawdust supplemented with cheap waste, sugar beet molasses still age. Advanced multiple response optimization techniques were applied to improve ligninolytic and reduce hydrolytic activity as a requirement for potential biorefinery use. The applied techniques were supposed to select cultivation conditions that would give manganese peroxidase and laccase activities above 0.84 and 0.12 U g(-1) substrate, respectively, and cellulase and xylanase activities below 1.12 and 1.4 U g(-1) substrate. The optimal cultivation conditions that met the set requirements included molasses stillage concentration of 10 %, substrate moisture content of 53 %, incubation temperature of 23.5 degrees C, and pH 5.2. The research showed that the addition of molasses stillage had a positive effect on enzyme production and that the optimal stillage concentration differed depending on the enzyme type (for laccase it was lt 5 %, manganese peroxidase approximate to 12 %, cellulase approximate to 21 % and xylanase approximate to 16 %), which should be taken into consideration when optimizing the desired process.. T2 - Journal of the Serbian Chemical Society T1 - Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage DO - 10.2298/JSC200601075J ER -
@article{ author = "Jovic, Jelena and Hao, Jian and Mojović, Ljiljana", year = "2022", abstract = "This study provides a detailed analysis of the lignocellulolytic activity of a new isolate Stereum gausapatum F28, a Serbian autochthonous fungi, on beechwood sawdust supplemented with cheap waste, sugar beet molasses still age. Advanced multiple response optimization techniques were applied to improve ligninolytic and reduce hydrolytic activity as a requirement for potential biorefinery use. The applied techniques were supposed to select cultivation conditions that would give manganese peroxidase and laccase activities above 0.84 and 0.12 U g(-1) substrate, respectively, and cellulase and xylanase activities below 1.12 and 1.4 U g(-1) substrate. The optimal cultivation conditions that met the set requirements included molasses stillage concentration of 10 %, substrate moisture content of 53 %, incubation temperature of 23.5 degrees C, and pH 5.2. The research showed that the addition of molasses stillage had a positive effect on enzyme production and that the optimal stillage concentration differed depending on the enzyme type (for laccase it was lt 5 %, manganese peroxidase approximate to 12 %, cellulase approximate to 21 % and xylanase approximate to 16 %), which should be taken into consideration when optimizing the desired process..", journal = "Journal of the Serbian Chemical Society", title = "Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage", doi = "10.2298/JSC200601075J" }
Jovic, J., Hao, J.,& Mojović, L.. (2022). Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage. in Journal of the Serbian Chemical Society. https://doi.org/10.2298/JSC200601075J
Jovic J, Hao J, Mojović L. Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage. in Journal of the Serbian Chemical Society. 2022;. doi:10.2298/JSC200601075J .
Jovic, Jelena, Hao, Jian, Mojović, Ljiljana, "Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage" in Journal of the Serbian Chemical Society (2022), https://doi.org/10.2298/JSC200601075J . .