Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity
Аутори
Veljović, ĐorđeMatić, Tamara
Stamenić, Tanja
Kojić, Vesna
Dimitrijević-Branković, Suzana
Lukić, Miodrag J.
Jevtić, Sanja
Radovanović, Željko
Petrović, Rada
Janaćković, Đorđe
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to improve the mechanical properties and to optimize antimicrobial activity of hydroxyapatite (HAP) by simultaneous doping with Mg and Cu ions in order to obtain material that would be able to assist in the bone/tooth healing process, prevent post-implementation infections and provide satisfying values of hardness and fracture toughness for biomedical application. Ion doping was done during the hydrothermal synthesis of HAP powders, whereby the content of Mg ions in the starting solution was varied between 1-20 mol. % with regard to Ca ions, while the amount of Cu ions was kept constant at 0.4 mol. %. The green compacts were sintered for 2 h at temperatures ranging 750–1200 °C depending on the Mg content, chosen in agreement with dilatometry results. Presence of Mg ions was found to favour transition from HAP to β−tricalcium phosphate phase (β−TCP), which enabled formation of biphasic HAP/β−TCP and pure β−TCP phase at 160 °C during hydrothermal synthesis. In v...itro investigation of antimicrobial activity against Escherichia coli, Staphylococcus aureus and Enterococcus faecalis showed satisfactory antimicrobial activity. MTT assay performed on MRC-5 and L929 cell lines showed excellent cytocompatibility and cell proliferation. Maximum hardness by Vickers and fracture toughness values, 4.96 GPa and 1.75 MPa m1/2 respectively, were obtained upon addition of 5 mol. % Mg, as a consequence of the lowest grain size and porosity, as well as the highest densification rate. This is, to the best of our knowledge, the highest fracture toughness for HAP or β-TCP ceramics reported thus far.
Кључне речи:
Biomedical application / Hydroxyapatite / Mechanical properties / SinteringИзвор:
Ceramics International, 2019, 45, 17, 22029-22039Издавач:
- Elsevier Ltd
Финансирање / пројекти:
- Синтеза, развој технологија добијања и примена наноструктурних мултифункционалних материјала дефинисаних својстава (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45019)
Напомена:
- Published version: http://technorep.tmf.bg.ac.rs/handle/123456789/4126
- This is the peer-reviewed version of the following article: Veljović Đ, Matić T, Stamenić T, Kojić V, Dimitrijević-Branković S, Lukić MJ, Jevtić S, Radovanović Ž, Petrović R, Janaćković Đ. Mg/Cu co-substituted hydroxyapatite - Biocompatibility, mechanical properties and antimicrobial activity. in Ceramics International. 2019;45(17):22029-22039. https://doi.org/10.1016/j.ceramint.2019.07.219
Повезане информације:
- Верзија документа
http://technorep.tmf.bg.ac.rs/handle/123456789/4126 - Верзија документа
https://doi.org/10.1016/j.ceramint.2019.07.219
DOI: 10.1016/j.ceramint.2019.07.219
ISSN: 0272-8842
WoS: 000493212500122
Scopus: 2-s2.0-85069563982
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Veljović, Đorđe AU - Matić, Tamara AU - Stamenić, Tanja AU - Kojić, Vesna AU - Dimitrijević-Branković, Suzana AU - Lukić, Miodrag J. AU - Jevtić, Sanja AU - Radovanović, Željko AU - Petrović, Rada AU - Janaćković, Đorđe PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5045 AB - The aim of this study was to improve the mechanical properties and to optimize antimicrobial activity of hydroxyapatite (HAP) by simultaneous doping with Mg and Cu ions in order to obtain material that would be able to assist in the bone/tooth healing process, prevent post-implementation infections and provide satisfying values of hardness and fracture toughness for biomedical application. Ion doping was done during the hydrothermal synthesis of HAP powders, whereby the content of Mg ions in the starting solution was varied between 1-20 mol. % with regard to Ca ions, while the amount of Cu ions was kept constant at 0.4 mol. %. The green compacts were sintered for 2 h at temperatures ranging 750–1200 °C depending on the Mg content, chosen in agreement with dilatometry results. Presence of Mg ions was found to favour transition from HAP to β−tricalcium phosphate phase (β−TCP), which enabled formation of biphasic HAP/β−TCP and pure β−TCP phase at 160 °C during hydrothermal synthesis. In vitro investigation of antimicrobial activity against Escherichia coli, Staphylococcus aureus and Enterococcus faecalis showed satisfactory antimicrobial activity. MTT assay performed on MRC-5 and L929 cell lines showed excellent cytocompatibility and cell proliferation. Maximum hardness by Vickers and fracture toughness values, 4.96 GPa and 1.75 MPa m1/2 respectively, were obtained upon addition of 5 mol. % Mg, as a consequence of the lowest grain size and porosity, as well as the highest densification rate. This is, to the best of our knowledge, the highest fracture toughness for HAP or β-TCP ceramics reported thus far. PB - Elsevier Ltd T2 - Ceramics International T1 - Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity EP - 22039 IS - 17 SP - 22029 VL - 45 DO - 10.1016/j.ceramint.2019.07.219 ER -
@article{ author = "Veljović, Đorđe and Matić, Tamara and Stamenić, Tanja and Kojić, Vesna and Dimitrijević-Branković, Suzana and Lukić, Miodrag J. and Jevtić, Sanja and Radovanović, Željko and Petrović, Rada and Janaćković, Đorđe", year = "2019", abstract = "The aim of this study was to improve the mechanical properties and to optimize antimicrobial activity of hydroxyapatite (HAP) by simultaneous doping with Mg and Cu ions in order to obtain material that would be able to assist in the bone/tooth healing process, prevent post-implementation infections and provide satisfying values of hardness and fracture toughness for biomedical application. Ion doping was done during the hydrothermal synthesis of HAP powders, whereby the content of Mg ions in the starting solution was varied between 1-20 mol. % with regard to Ca ions, while the amount of Cu ions was kept constant at 0.4 mol. %. The green compacts were sintered for 2 h at temperatures ranging 750–1200 °C depending on the Mg content, chosen in agreement with dilatometry results. Presence of Mg ions was found to favour transition from HAP to β−tricalcium phosphate phase (β−TCP), which enabled formation of biphasic HAP/β−TCP and pure β−TCP phase at 160 °C during hydrothermal synthesis. In vitro investigation of antimicrobial activity against Escherichia coli, Staphylococcus aureus and Enterococcus faecalis showed satisfactory antimicrobial activity. MTT assay performed on MRC-5 and L929 cell lines showed excellent cytocompatibility and cell proliferation. Maximum hardness by Vickers and fracture toughness values, 4.96 GPa and 1.75 MPa m1/2 respectively, were obtained upon addition of 5 mol. % Mg, as a consequence of the lowest grain size and porosity, as well as the highest densification rate. This is, to the best of our knowledge, the highest fracture toughness for HAP or β-TCP ceramics reported thus far.", publisher = "Elsevier Ltd", journal = "Ceramics International", title = "Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity", pages = "22039-22029", number = "17", volume = "45", doi = "10.1016/j.ceramint.2019.07.219" }
Veljović, Đ., Matić, T., Stamenić, T., Kojić, V., Dimitrijević-Branković, S., Lukić, M. J., Jevtić, S., Radovanović, Ž., Petrović, R.,& Janaćković, Đ.. (2019). Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity. in Ceramics International Elsevier Ltd., 45(17), 22029-22039. https://doi.org/10.1016/j.ceramint.2019.07.219
Veljović Đ, Matić T, Stamenić T, Kojić V, Dimitrijević-Branković S, Lukić MJ, Jevtić S, Radovanović Ž, Petrović R, Janaćković Đ. Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity. in Ceramics International. 2019;45(17):22029-22039. doi:10.1016/j.ceramint.2019.07.219 .
Veljović, Đorđe, Matić, Tamara, Stamenić, Tanja, Kojić, Vesna, Dimitrijević-Branković, Suzana, Lukić, Miodrag J., Jevtić, Sanja, Radovanović, Željko, Petrović, Rada, Janaćković, Đorđe, "Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity" in Ceramics International, 45, no. 17 (2019):22029-22039, https://doi.org/10.1016/j.ceramint.2019.07.219 . .