Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity
Аутори
Lukić, IvanaMilovanović, Stoja
Pantić, Milica
Srbljak, Ivana
Djurić, Ana
Tadić, Vanja
Tyśkiewicz, Katarzyna
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Extracts from Silybum marianum seeds have high industrial potential due to their application as phytopharmaceuticals and food. In order to optimize its production, conventional extraction (in Soxhlet apparatus using solvents ethanol and n-hexane) and supercritical fluid extraction (using CO2 at the pressure of 30 MPa and temperature of 40 °C with and without co-solvent ethanol, from oil-rich and defatted seeds) were compared. In addition, the effect of plant cultivation (years 2011 and 2019) and the effect of seeds and extract storage on the yield and quality of extracts were assessed. It was shown that unsaturated fatty acids (linoleic and oleic acids being most dominant) constituted 64–87% of obtained extracts. Content of α-tocopherol varied from 0.01 to 13 g/100 g, while total phenolic content was in the range from 13.2 to 104.2 gGAE/100 gextract. In vitro cytotoxic activity analysis confirmed that extracts obtained from defatted seeds showed activity against cancer cells. This stud...y pointed out the important role of the cultivation year, selection of extraction technique and solvent, as well as storage on the extraction yield, chemical profile, and cytotoxic activity of extracts.
Кључне речи:
Cytotoxic activity / Linoleic acid / Milk thistle / Supercritical CO2 / α-tocopherolИзвор:
LWT, 2022, 160, 113319-Издавач:
- Academic Press
Финансирање / пројекти:
DOI: 10.1016/j.lwt.2022.113319
ISSN: 0023-6438
WoS: 00080221430000
Scopus: 2-s2.0-85125923575
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Lukić, Ivana AU - Milovanović, Stoja AU - Pantić, Milica AU - Srbljak, Ivana AU - Djurić, Ana AU - Tadić, Vanja AU - Tyśkiewicz, Katarzyna PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5093 AB - Extracts from Silybum marianum seeds have high industrial potential due to their application as phytopharmaceuticals and food. In order to optimize its production, conventional extraction (in Soxhlet apparatus using solvents ethanol and n-hexane) and supercritical fluid extraction (using CO2 at the pressure of 30 MPa and temperature of 40 °C with and without co-solvent ethanol, from oil-rich and defatted seeds) were compared. In addition, the effect of plant cultivation (years 2011 and 2019) and the effect of seeds and extract storage on the yield and quality of extracts were assessed. It was shown that unsaturated fatty acids (linoleic and oleic acids being most dominant) constituted 64–87% of obtained extracts. Content of α-tocopherol varied from 0.01 to 13 g/100 g, while total phenolic content was in the range from 13.2 to 104.2 gGAE/100 gextract. In vitro cytotoxic activity analysis confirmed that extracts obtained from defatted seeds showed activity against cancer cells. This study pointed out the important role of the cultivation year, selection of extraction technique and solvent, as well as storage on the extraction yield, chemical profile, and cytotoxic activity of extracts. PB - Academic Press T2 - LWT T1 - Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity SP - 113319 VL - 160 DO - 10.1016/j.lwt.2022.113319 ER -
@article{ author = "Lukić, Ivana and Milovanović, Stoja and Pantić, Milica and Srbljak, Ivana and Djurić, Ana and Tadić, Vanja and Tyśkiewicz, Katarzyna", year = "2022", abstract = "Extracts from Silybum marianum seeds have high industrial potential due to their application as phytopharmaceuticals and food. In order to optimize its production, conventional extraction (in Soxhlet apparatus using solvents ethanol and n-hexane) and supercritical fluid extraction (using CO2 at the pressure of 30 MPa and temperature of 40 °C with and without co-solvent ethanol, from oil-rich and defatted seeds) were compared. In addition, the effect of plant cultivation (years 2011 and 2019) and the effect of seeds and extract storage on the yield and quality of extracts were assessed. It was shown that unsaturated fatty acids (linoleic and oleic acids being most dominant) constituted 64–87% of obtained extracts. Content of α-tocopherol varied from 0.01 to 13 g/100 g, while total phenolic content was in the range from 13.2 to 104.2 gGAE/100 gextract. In vitro cytotoxic activity analysis confirmed that extracts obtained from defatted seeds showed activity against cancer cells. This study pointed out the important role of the cultivation year, selection of extraction technique and solvent, as well as storage on the extraction yield, chemical profile, and cytotoxic activity of extracts.", publisher = "Academic Press", journal = "LWT", title = "Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity", pages = "113319", volume = "160", doi = "10.1016/j.lwt.2022.113319" }
Lukić, I., Milovanović, S., Pantić, M., Srbljak, I., Djurić, A., Tadić, V.,& Tyśkiewicz, K.. (2022). Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity. in LWT Academic Press., 160, 113319. https://doi.org/10.1016/j.lwt.2022.113319
Lukić I, Milovanović S, Pantić M, Srbljak I, Djurić A, Tadić V, Tyśkiewicz K. Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity. in LWT. 2022;160:113319. doi:10.1016/j.lwt.2022.113319 .
Lukić, Ivana, Milovanović, Stoja, Pantić, Milica, Srbljak, Ivana, Djurić, Ana, Tadić, Vanja, Tyśkiewicz, Katarzyna, "Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity" in LWT, 160 (2022):113319, https://doi.org/10.1016/j.lwt.2022.113319 . .