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Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production
dc.creator | Wang, Qinghui | |
dc.creator | Gu, Jinjie | |
dc.creator | Shu, Lin | |
dc.creator | Jiang, Weiyan | |
dc.creator | Mojović, Ljiljana | |
dc.creator | Knežević-Jugović, Zorica | |
dc.creator | Shi, Jiping | |
dc.creator | Baganz, Frank | |
dc.creator | Lye, Gary J. | |
dc.creator | Xiang, Wensheng | |
dc.creator | Hao, Jian | |
dc.date.accessioned | 2022-05-18T10:18:32Z | |
dc.date.available | 2022-05-18T10:18:32Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 0959-3993 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5109 | |
dc.description.abstract | Klebsiella pneumoniae is a 2,3-butanediol producing bacterium. Nevertheless, a design and construction of l-valine production strain was studied in this paper. The first step of 2,3-butanediol synthesis and branched-chain amino acid synthesis pathways share the same step of α-acetolactate synthesis from pyruvate. However, the two pathways are existing in parallel and do not interfere with each other in the wild-type strain. A knockout of budA blocked the 2,3-butanediol synthesis pathway and resulted in the l-valine production. The budA coded an α-acetolactate decarboxylase and catalyzed the acetoin formation from α-acetolactate. Furthermore, blocking the lactic acid synthesis by knocking out of ldhA, which is encoding a lactate dehydrogenase, improved the l-valine synthesis. 2-Ketoisovalerate is the precursor of l-valine, it is also an intermediate of the isobutanol synthesis pathway, while indole-3-pyruvate decarboxylase (ipdC) is responsible for isobutyraldehyde formation from 2-ketoisovalerate. Production of l-valine has been improved by knocking out of ipdC. On the other side, the ilvE, encoding a transaminase B, reversibly transfers one amino group from glutamate to α-ketoisovalerate. Overexpression of ilvE exhibited a distinct improvement of l-valine production. The brnQ encodes a branched-chain amino acid transporter, and l-valine production was further improved by disrupting brnQ. It is also revealed that weak acidic and aerobic conditions favor l-valine production. Based on these findings, l-valine production by metabolically engineered K. pneumonia was examined. In fed-batch fermentation, 22.4 g/L of l-valine was produced by the engineered K. pneumoniae ΔbudA-ΔldhA-ΔipdC-ΔbrnQ-ilvE after 55 h of cultivation, with a substrate conversion ratio of 0.27 mol/mol glucose. | sr |
dc.language.iso | en | sr |
dc.publisher | Springer Science and Business Media B.V. | sr |
dc.rights | restrictedAccess | sr |
dc.source | World Journal of Microbiology and Biotechnology | sr |
dc.subject | 2,3-Butanediol | sr |
dc.subject | brnQ | sr |
dc.subject | ilvE | sr |
dc.subject | ipdC | sr |
dc.subject | Klebsiella pneumoniae | sr |
dc.subject | l-valine | sr |
dc.title | Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production | sr |
dc.type | article | sr |
dc.rights.license | ARR | sr |
dc.citation.issue | 5 | |
dc.citation.rank | M22~ | |
dc.citation.spage | 81 | |
dc.citation.volume | 38 | |
dc.identifier.doi | 10.1007/s11274-022-03266-9 | |
dc.identifier.scopus | 2-s2.0-85127284900 | |
dc.identifier.wos | 000775117100001 | |
dc.type.version | publishedVersion | sr |