Periodate oxidized horseradish peroxidase@ZIF-8 nanocomposite

Abstract

Metal-organic frameworks (MOFs) are a class of materials well-known for their high degree of crystallinity andultrahigh porosity. Modular synthesis from organic linkers and metal nodes allows for precise control of structure,pore size and chemical functionality of MOFs. Recently, MOFs have been explored for their potential to form novelbiocomposites with proteins by a process termed biomimetic mineralization. These novel MOF biocomposites showgreat promise for application to industrial biocatalysis where strategies for enhancing enzyme stability are ofsignificant interest. The protective capacity and applications of biomimetically mineralized biomacromolecule zeoliticimidazolate framework (ZIF-8) composites are likely dependent on the charge of the biomolecule and the topologyof the mineralized ZIF-8 coating. Herein, we identify conditions to reliably yield the porous periodate oxidizedhorseradish peroxidase@ZIF-8 sodalite topology biocomposite in preference to other more dense phases.