Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment

Abstract

Immobilization of lipase B from Candida antarctica was carried out using three different immobilization methods, and two epoxy activated supports, comercial Eupergit C and synthesized macroporous copolymer of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA). The synthesized polymer was proven to be better for CALB immobilization at both ionic strenghts that wrere applied, 0.1M and 1.25M. Investigation of the effect of immobilization pH on the activity of immobilized enzymes, showed that the highest activities were obtained when immobilization process was conducted in acidic enviorments. The highest immobilized enzyme activity, 30.5 IU/ g of dry support, was achieved when periodate method was employed. Glutaraldehyde activation of support did not improve activity of obtained immobilized enzymes.