Screening for factors affecting cellulose adsorption from solutions by modified coffee residues

Abstract

In the present study, the modified coffee residues were used as low cost adsorbent for immobilization of cellulase enzyme. In the batch model for adsorption, the experimental parameters: the temperature, the initial cellulase concentration and the agitation rate of adsorption process were investigated. The maximal percentages of immobilization yield (approximately 28%) and immobilization efficiency (approximately 48%) were obtained with the temperature of 40 °C, the initial cellulase concentration of 1 mg/mL and the agitation rate of 120 rpm. The state of dynamic equilibrium was reached after 60 minutes and the pseudo-second-order model was predicted to follow the process kinetic.