Cellulolytic potencial of a strain Paenibacillus sp. isolated from soil
Аутори
Mihajlovski, KatarinaDavidović, Slađana
Miljković, Miona
Rajilić-Stojanović, Mirjana
Dimitrijević-Branković, Suzana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Cellulases are the enzymes which cause hydrolyzis of the polysaccharide cellulose into
smaller sugar units. Bacterial cellulases possess more advantages when compared to the
cellulases from other sources. The gram positive, spore forming bacteria was isolated from
soil. Strain was identified as Paenibacillus chitinolyticus after biochemical and 16S rRNA
sequencing studies. The isolated strain was screened for cellulase activity by using a
Gram’s iodine metod on carboxymethyl cellulose (CMC) agar plate [1]. It has been found
to produce cellulases which could liberate glucose from CMC (CMC-ase) and
microcristalline cellulose Avicel (avicelase). The enzyme activity was measured by
estimating the liberated reducing sugars using 3,5-dinitrosalicylic acid method [2].
A liquid medium containing 0.5% CMC and yeast extract proved to be a suitable carbon
and nitrogen sources for cellulase production by the bacterium. Maximal cellulase
production for CMC-ase 0.085 U/ml and for avicelas...e 0.17 U/ml was obtained after 48 h of
incubation at 30 °C under aerobic conditions with 5% overnight culture and with addition
of 0.5% CMC, 10 g/l yeast extract, MgSO4 0.5g/l, KCl 0.5g/l, CaCl2 0.02g/l and 1g/l Tween
80.
Paenibacillus sp. strain isolated from soil is available for further studies aiming the
optimization of its cellulase production, facilitating its potential use in industrial
applications.
Извор:
8th International Conference of the Chemical Societies of the South-East European Countries - ICOSECS 8, 2013, 244-Издавач:
- Serbian Chemical Society
Финансирање / пројекти:
- Примена биотехнолошких метода у одрживом искоришћењу нус-производа агроиндустрије (RS-MESTD-Technological Development (TD or TR)-31035)
Институција/група
Tehnološko-metalurški fakultetTY - CONF AU - Mihajlovski, Katarina AU - Davidović, Slađana AU - Miljković, Miona AU - Rajilić-Stojanović, Mirjana AU - Dimitrijević-Branković, Suzana PY - 2013 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6160 AB - Cellulases are the enzymes which cause hydrolyzis of the polysaccharide cellulose into smaller sugar units. Bacterial cellulases possess more advantages when compared to the cellulases from other sources. The gram positive, spore forming bacteria was isolated from soil. Strain was identified as Paenibacillus chitinolyticus after biochemical and 16S rRNA sequencing studies. The isolated strain was screened for cellulase activity by using a Gram’s iodine metod on carboxymethyl cellulose (CMC) agar plate [1]. It has been found to produce cellulases which could liberate glucose from CMC (CMC-ase) and microcristalline cellulose Avicel (avicelase). The enzyme activity was measured by estimating the liberated reducing sugars using 3,5-dinitrosalicylic acid method [2]. A liquid medium containing 0.5% CMC and yeast extract proved to be a suitable carbon and nitrogen sources for cellulase production by the bacterium. Maximal cellulase production for CMC-ase 0.085 U/ml and for avicelase 0.17 U/ml was obtained after 48 h of incubation at 30 °C under aerobic conditions with 5% overnight culture and with addition of 0.5% CMC, 10 g/l yeast extract, MgSO4 0.5g/l, KCl 0.5g/l, CaCl2 0.02g/l and 1g/l Tween 80. Paenibacillus sp. strain isolated from soil is available for further studies aiming the optimization of its cellulase production, facilitating its potential use in industrial applications. PB - Serbian Chemical Society C3 - 8th International Conference of the Chemical Societies of the South-East European Countries - ICOSECS 8 T1 - Cellulolytic potencial of a strain Paenibacillus sp. isolated from soil SP - 244 UR - https://hdl.handle.net/21.15107/rcub_technorep_6160 ER -
@conference{ author = "Mihajlovski, Katarina and Davidović, Slađana and Miljković, Miona and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana", year = "2013", abstract = "Cellulases are the enzymes which cause hydrolyzis of the polysaccharide cellulose into smaller sugar units. Bacterial cellulases possess more advantages when compared to the cellulases from other sources. The gram positive, spore forming bacteria was isolated from soil. Strain was identified as Paenibacillus chitinolyticus after biochemical and 16S rRNA sequencing studies. The isolated strain was screened for cellulase activity by using a Gram’s iodine metod on carboxymethyl cellulose (CMC) agar plate [1]. It has been found to produce cellulases which could liberate glucose from CMC (CMC-ase) and microcristalline cellulose Avicel (avicelase). The enzyme activity was measured by estimating the liberated reducing sugars using 3,5-dinitrosalicylic acid method [2]. A liquid medium containing 0.5% CMC and yeast extract proved to be a suitable carbon and nitrogen sources for cellulase production by the bacterium. Maximal cellulase production for CMC-ase 0.085 U/ml and for avicelase 0.17 U/ml was obtained after 48 h of incubation at 30 °C under aerobic conditions with 5% overnight culture and with addition of 0.5% CMC, 10 g/l yeast extract, MgSO4 0.5g/l, KCl 0.5g/l, CaCl2 0.02g/l and 1g/l Tween 80. Paenibacillus sp. strain isolated from soil is available for further studies aiming the optimization of its cellulase production, facilitating its potential use in industrial applications.", publisher = "Serbian Chemical Society", journal = "8th International Conference of the Chemical Societies of the South-East European Countries - ICOSECS 8", title = "Cellulolytic potencial of a strain Paenibacillus sp. isolated from soil", pages = "244", url = "https://hdl.handle.net/21.15107/rcub_technorep_6160" }
Mihajlovski, K., Davidović, S., Miljković, M., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2013). Cellulolytic potencial of a strain Paenibacillus sp. isolated from soil. in 8th International Conference of the Chemical Societies of the South-East European Countries - ICOSECS 8 Serbian Chemical Society., 244. https://hdl.handle.net/21.15107/rcub_technorep_6160
Mihajlovski K, Davidović S, Miljković M, Rajilić-Stojanović M, Dimitrijević-Branković S. Cellulolytic potencial of a strain Paenibacillus sp. isolated from soil. in 8th International Conference of the Chemical Societies of the South-East European Countries - ICOSECS 8. 2013;:244. https://hdl.handle.net/21.15107/rcub_technorep_6160 .
Mihajlovski, Katarina, Davidović, Slađana, Miljković, Miona, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Cellulolytic potencial of a strain Paenibacillus sp. isolated from soil" in 8th International Conference of the Chemical Societies of the South-East European Countries - ICOSECS 8 (2013):244, https://hdl.handle.net/21.15107/rcub_technorep_6160 .