Screening for factors affecting dextransucrase production from Leuconostoc mesenteroides isolated from water kefir grains using statistical approach
Само за регистроване кориснике
2013
Аутори
Miljković, MionaDavidović, Slađana
Rajilić-Stojanović, Mirjana
Šiler-Marinković, Slavica S.
Dimitrijević-Branković, Suzana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A leuconostoc strain was isolated from water kefir grains obtained from a household in Belgrade, Serbia. Strain was identified as Leuconostoc mesenteroides after biochemical and 16S rRNA sequencing studies. The isolated strain was found to produce dextransucrase. Culture medium and growth conditions for enzyme production were screened and optimized using the statistical design technique of Plackett-Burman [1]. Plackett-Burman design with variables viz. sucrose, yeast extract, beef extract, K2HPO4, Tween 80, incubation time, temperature and inoculum, was performed to screen main factors affecting enzyme production. Based on factorial design seventeen experiments were performed. Each factor was examined at two levels: -1 for low level and +1 for high level, and five center points were run to evaluate the linear and curvature effects of the variables.
The enzyme activity was measured by estimating the liberated reducing sugars using 3,5-dinitrosalicylic acid method [2].
Among tested fac...tors positive effect gave K2HPO4, Tween 80 and sucrose. Dextransucrase activity varied from 0.52 to 4.81 U/ml. Maximum activity was acquired from medium contained 50 g/l sucrose, 10 g/l yeast extract, 15 g/l beef extract , 60 g/l K2HPO4, 2g/l Tween 80, inoculated with 2% overnight culture and cultivated at 23oC for 16h.
The resultats from statistical design technique of Plackett-Burman show that tested strain has high potential for dextransucrase production. Further optimization with positive factors should be done by the other statistical design technique, response surface methodology.
Извор:
ICOSECS 8 8th International Conference of the Chemical Societies of the South-East European Countries, 2013, 264-Издавач:
- Serbian Chemical Society
Финансирање / пројекти:
- Примена биотехнолошких метода у одрживом искоришћењу нус-производа агроиндустрије (RS-MESTD-Technological Development (TD or TR)-31035)
Институција/група
Tehnološko-metalurški fakultetTY - CONF AU - Miljković, Miona AU - Davidović, Slađana AU - Rajilić-Stojanović, Mirjana AU - Šiler-Marinković, Slavica S. AU - Dimitrijević-Branković, Suzana PY - 2013 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6207 AB - A leuconostoc strain was isolated from water kefir grains obtained from a household in Belgrade, Serbia. Strain was identified as Leuconostoc mesenteroides after biochemical and 16S rRNA sequencing studies. The isolated strain was found to produce dextransucrase. Culture medium and growth conditions for enzyme production were screened and optimized using the statistical design technique of Plackett-Burman [1]. Plackett-Burman design with variables viz. sucrose, yeast extract, beef extract, K2HPO4, Tween 80, incubation time, temperature and inoculum, was performed to screen main factors affecting enzyme production. Based on factorial design seventeen experiments were performed. Each factor was examined at two levels: -1 for low level and +1 for high level, and five center points were run to evaluate the linear and curvature effects of the variables. The enzyme activity was measured by estimating the liberated reducing sugars using 3,5-dinitrosalicylic acid method [2]. Among tested factors positive effect gave K2HPO4, Tween 80 and sucrose. Dextransucrase activity varied from 0.52 to 4.81 U/ml. Maximum activity was acquired from medium contained 50 g/l sucrose, 10 g/l yeast extract, 15 g/l beef extract , 60 g/l K2HPO4, 2g/l Tween 80, inoculated with 2% overnight culture and cultivated at 23oC for 16h. The resultats from statistical design technique of Plackett-Burman show that tested strain has high potential for dextransucrase production. Further optimization with positive factors should be done by the other statistical design technique, response surface methodology. PB - Serbian Chemical Society C3 - ICOSECS 8 8th International Conference of the Chemical Societies of the South-East European Countries T1 - Screening for factors affecting dextransucrase production from Leuconostoc mesenteroides isolated from water kefir grains using statistical approach SP - 264 UR - https://hdl.handle.net/21.15107/rcub_technorep_6207 ER -
@conference{ author = "Miljković, Miona and Davidović, Slađana and Rajilić-Stojanović, Mirjana and Šiler-Marinković, Slavica S. and Dimitrijević-Branković, Suzana", year = "2013", abstract = "A leuconostoc strain was isolated from water kefir grains obtained from a household in Belgrade, Serbia. Strain was identified as Leuconostoc mesenteroides after biochemical and 16S rRNA sequencing studies. The isolated strain was found to produce dextransucrase. Culture medium and growth conditions for enzyme production were screened and optimized using the statistical design technique of Plackett-Burman [1]. Plackett-Burman design with variables viz. sucrose, yeast extract, beef extract, K2HPO4, Tween 80, incubation time, temperature and inoculum, was performed to screen main factors affecting enzyme production. Based on factorial design seventeen experiments were performed. Each factor was examined at two levels: -1 for low level and +1 for high level, and five center points were run to evaluate the linear and curvature effects of the variables. The enzyme activity was measured by estimating the liberated reducing sugars using 3,5-dinitrosalicylic acid method [2]. Among tested factors positive effect gave K2HPO4, Tween 80 and sucrose. Dextransucrase activity varied from 0.52 to 4.81 U/ml. Maximum activity was acquired from medium contained 50 g/l sucrose, 10 g/l yeast extract, 15 g/l beef extract , 60 g/l K2HPO4, 2g/l Tween 80, inoculated with 2% overnight culture and cultivated at 23oC for 16h. The resultats from statistical design technique of Plackett-Burman show that tested strain has high potential for dextransucrase production. Further optimization with positive factors should be done by the other statistical design technique, response surface methodology.", publisher = "Serbian Chemical Society", journal = "ICOSECS 8 8th International Conference of the Chemical Societies of the South-East European Countries", title = "Screening for factors affecting dextransucrase production from Leuconostoc mesenteroides isolated from water kefir grains using statistical approach", pages = "264", url = "https://hdl.handle.net/21.15107/rcub_technorep_6207" }
Miljković, M., Davidović, S., Rajilić-Stojanović, M., Šiler-Marinković, S. S.,& Dimitrijević-Branković, S.. (2013). Screening for factors affecting dextransucrase production from Leuconostoc mesenteroides isolated from water kefir grains using statistical approach. in ICOSECS 8 8th International Conference of the Chemical Societies of the South-East European Countries Serbian Chemical Society., 264. https://hdl.handle.net/21.15107/rcub_technorep_6207
Miljković M, Davidović S, Rajilić-Stojanović M, Šiler-Marinković SS, Dimitrijević-Branković S. Screening for factors affecting dextransucrase production from Leuconostoc mesenteroides isolated from water kefir grains using statistical approach. in ICOSECS 8 8th International Conference of the Chemical Societies of the South-East European Countries. 2013;:264. https://hdl.handle.net/21.15107/rcub_technorep_6207 .
Miljković, Miona, Davidović, Slađana, Rajilić-Stojanović, Mirjana, Šiler-Marinković, Slavica S., Dimitrijević-Branković, Suzana, "Screening for factors affecting dextransucrase production from Leuconostoc mesenteroides isolated from water kefir grains using statistical approach" in ICOSECS 8 8th International Conference of the Chemical Societies of the South-East European Countries (2013):264, https://hdl.handle.net/21.15107/rcub_technorep_6207 .