Removal of Synthetic Dyes from Wastewaters Using Horseradish Peroxidase
Само за регистроване кориснике
2019
Поглавље у монографији (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Horseradish peroxidase is a heme-containing plant enzyme (HRP; EC 1.11.1.7) with a wide range of biotechnological and medical applications. In combination with hydrogen peroxide (H2O2) as an oxidant HRP can be used in the oxidation of various substrates (e.g. considered as toxic, muthagenic, recalcitrant and carcinogenic compounds with harmful impact on human health and ecosystems and main textile industry problem when it comes to environment pollution with wastewaters. Traditional methods are inefficient, and in recent years, with a view to preserving the environment, the scientific community turns to the application of enzymes as the main catalysts in the removal of synthetic dyes. Based on the data available in the literature, HRP proved to be an enzyme with a high potential for the application of synthetic dyes removal by the mechanism of polymerization or ring opening. In this chapter well-studied enzyme is described with the focus on its promising use in colored wastewater treatm...ent. C. I. Acid Violet 109 (AV 109) was used as a model dye in order to examine the feasibility of HRP application in removal of anthraquinone class of synthetic dyes. The reaction conditions were optimized in terms of dye, enzyme and H2O2 concentration as well as temperature and pH influence. It was established that the dye removal shows good agreement with two substrate ping-pong mechanism with substrate inhibition. The industrial application of this enzyme requires the economic feasibility. Accordingly, an expensive commercial preparation HRP was replaced with partially purified enzyme isolated from horseradish root which showed high efficiency in model dye removal. The next step towards the industrial application of this enzyme was the immobilization of various techniques (adsorption, covalent immobilization, cross-linked aggregates) to various carriers such as kaolin and the synthetic carrier Purolite® A109. Improvement in storage and operational stability after immobilization was confirmed and immobilized preparations were applied in different bioreactor configurations, batch and packed bed. HRP catalyzed removal of synthetic dyes appeared to be promising technique dye to high efficiency in dye removal and phytotoxicity and acute toxicity decrease, measured by Mung bean seeds and A. salina assays.
Кључне речи:
horseradish peroxidase / wastewater / synthetic dyes / immobilization / toxicity / enzymatic catalysis / Ping-Pong Bi-BiИзвор:
Horseradish peroxidase: Structure, Functions and Applications, 2019, 112-168Издавач:
- New York: Nova Science Publishers, Inc.
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-46010)
- Проучавање синтезе, структуре и активности органских једињења природног и синтетског порекла (RS-172013)
- Електрохемијска синтеза и карактеризација наноструктурираних функционалних материјала за примену у новим технологијама (RS-172046)
Колекције
Институција/група
Inovacioni centarTY - CHAP AU - Šekuljica, Nataša AU - Prlainović, Nevena AU - Knežević-Jugović, Zorica AU - Mijin, Dušan PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6269 AB - Horseradish peroxidase is a heme-containing plant enzyme (HRP; EC 1.11.1.7) with a wide range of biotechnological and medical applications. In combination with hydrogen peroxide (H2O2) as an oxidant HRP can be used in the oxidation of various substrates (e.g. considered as toxic, muthagenic, recalcitrant and carcinogenic compounds with harmful impact on human health and ecosystems and main textile industry problem when it comes to environment pollution with wastewaters. Traditional methods are inefficient, and in recent years, with a view to preserving the environment, the scientific community turns to the application of enzymes as the main catalysts in the removal of synthetic dyes. Based on the data available in the literature, HRP proved to be an enzyme with a high potential for the application of synthetic dyes removal by the mechanism of polymerization or ring opening. In this chapter well-studied enzyme is described with the focus on its promising use in colored wastewater treatment. C. I. Acid Violet 109 (AV 109) was used as a model dye in order to examine the feasibility of HRP application in removal of anthraquinone class of synthetic dyes. The reaction conditions were optimized in terms of dye, enzyme and H2O2 concentration as well as temperature and pH influence. It was established that the dye removal shows good agreement with two substrate ping-pong mechanism with substrate inhibition. The industrial application of this enzyme requires the economic feasibility. Accordingly, an expensive commercial preparation HRP was replaced with partially purified enzyme isolated from horseradish root which showed high efficiency in model dye removal. The next step towards the industrial application of this enzyme was the immobilization of various techniques (adsorption, covalent immobilization, cross-linked aggregates) to various carriers such as kaolin and the synthetic carrier Purolite® A109. Improvement in storage and operational stability after immobilization was confirmed and immobilized preparations were applied in different bioreactor configurations, batch and packed bed. HRP catalyzed removal of synthetic dyes appeared to be promising technique dye to high efficiency in dye removal and phytotoxicity and acute toxicity decrease, measured by Mung bean seeds and A. salina assays. PB - New York: Nova Science Publishers, Inc. T2 - Horseradish peroxidase: Structure, Functions and Applications T1 - Removal of Synthetic Dyes from Wastewaters Using Horseradish Peroxidase EP - 168 SP - 112 UR - https://hdl.handle.net/21.15107/rcub_technorep_6269 ER -
@inbook{ author = "Šekuljica, Nataša and Prlainović, Nevena and Knežević-Jugović, Zorica and Mijin, Dušan", year = "2019", abstract = "Horseradish peroxidase is a heme-containing plant enzyme (HRP; EC 1.11.1.7) with a wide range of biotechnological and medical applications. In combination with hydrogen peroxide (H2O2) as an oxidant HRP can be used in the oxidation of various substrates (e.g. considered as toxic, muthagenic, recalcitrant and carcinogenic compounds with harmful impact on human health and ecosystems and main textile industry problem when it comes to environment pollution with wastewaters. Traditional methods are inefficient, and in recent years, with a view to preserving the environment, the scientific community turns to the application of enzymes as the main catalysts in the removal of synthetic dyes. Based on the data available in the literature, HRP proved to be an enzyme with a high potential for the application of synthetic dyes removal by the mechanism of polymerization or ring opening. In this chapter well-studied enzyme is described with the focus on its promising use in colored wastewater treatment. C. I. Acid Violet 109 (AV 109) was used as a model dye in order to examine the feasibility of HRP application in removal of anthraquinone class of synthetic dyes. The reaction conditions were optimized in terms of dye, enzyme and H2O2 concentration as well as temperature and pH influence. It was established that the dye removal shows good agreement with two substrate ping-pong mechanism with substrate inhibition. The industrial application of this enzyme requires the economic feasibility. Accordingly, an expensive commercial preparation HRP was replaced with partially purified enzyme isolated from horseradish root which showed high efficiency in model dye removal. The next step towards the industrial application of this enzyme was the immobilization of various techniques (adsorption, covalent immobilization, cross-linked aggregates) to various carriers such as kaolin and the synthetic carrier Purolite® A109. Improvement in storage and operational stability after immobilization was confirmed and immobilized preparations were applied in different bioreactor configurations, batch and packed bed. HRP catalyzed removal of synthetic dyes appeared to be promising technique dye to high efficiency in dye removal and phytotoxicity and acute toxicity decrease, measured by Mung bean seeds and A. salina assays.", publisher = "New York: Nova Science Publishers, Inc.", journal = "Horseradish peroxidase: Structure, Functions and Applications", booktitle = "Removal of Synthetic Dyes from Wastewaters Using Horseradish Peroxidase", pages = "168-112", url = "https://hdl.handle.net/21.15107/rcub_technorep_6269" }
Šekuljica, N., Prlainović, N., Knežević-Jugović, Z.,& Mijin, D.. (2019). Removal of Synthetic Dyes from Wastewaters Using Horseradish Peroxidase. in Horseradish peroxidase: Structure, Functions and Applications New York: Nova Science Publishers, Inc.., 112-168. https://hdl.handle.net/21.15107/rcub_technorep_6269
Šekuljica N, Prlainović N, Knežević-Jugović Z, Mijin D. Removal of Synthetic Dyes from Wastewaters Using Horseradish Peroxidase. in Horseradish peroxidase: Structure, Functions and Applications. 2019;:112-168. https://hdl.handle.net/21.15107/rcub_technorep_6269 .
Šekuljica, Nataša, Prlainović, Nevena, Knežević-Jugović, Zorica, Mijin, Dušan, "Removal of Synthetic Dyes from Wastewaters Using Horseradish Peroxidase" in Horseradish peroxidase: Structure, Functions and Applications (2019):112-168, https://hdl.handle.net/21.15107/rcub_technorep_6269 .