Primena džibre tritikalea za proizvodnju mlečne kiseline

Abstract

Triticale is a very applicable crop for bioethanol production, and also provides a stillage that can be used as a feed. The main aim of this research was to investigate the possibility of transformation of unused stillage carbohydrate after bioethanol prooduction into the lactis acid fermentation, in order to produce a natural feed preservative. In this study Lactobacillus fermentum Pl-1 was used. Different pre-treatment were done during the preparation of bioethanol fermentation (by triticale autoamylolytic enzymes, technical enzymes and ultrasound), and thus 4 different samples of stillage were prepared. CaCO3 was added in one part of the samples. Results indicated a good conversion factor of glucose into the lactic acid, up to 1.55 after 72 hours of the treatment. The sample pre-treated only by triticale autoamylolytic enzymes had a small part of carbohydrtes utilised during the stillage preparation. As a result, there was more glucose available for lactic acid fermentation. Added CaCo3 probably improved the protection of Lactobacillus fermentum PL-1 cells, but it was not possible to see a real glucose conversion factor due to Ca-lactate formation. Therefore, the stillage produced after the bioethanol production is a very good medium for lactic acid production