Development and optimization of the production procedure of biphasic scaffolds for osteochondral tissue engineering
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Scaffolds used for osteochondral tissue engineering should comprise two distinct regions: a
bottom region with characteristics corresponding to bone tissue, such as a porous structure
with mineral components (predominantly hydroxyapatite), and a top region with
characteristics of articular cartilage, which is gelatinous with high water content. In this
work, we have investigated possibilities to formulate and optimize a procedure for obtaining
such biphasic scaffolds based on gellan gum (GG). A porous base layer of the scaffold was
obtained by lyophilization of the 2 % GG hydrogel with dispersed bioactive glass
nanoparticles, as hydroxyapatite precursors. Next, different procedures were investigated to
produce the upper GG hydrogel such as partial immersion of the porous layer in the GG
solution and pouring the GG solution over the porous layer at different moisture conditions
and temperatures. A simple mathematical model was derived and subsequently
experimentally validated... to find optimal temperatures of the porous layer, GG solution and
the surrounding environment to provide adequate gelation rate to form the GG hydrogel on
top of the porous layer with a thin interfacial zone.
Извор:
Seventieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts, 2017, 4-Издавач:
- Institute of Technical Sciences of SASA
Институција/група
Inovacioni centarTY - CONF AU - Radonjić, Mia AU - Zvicer, Jovana AU - Obradović, Bojana PY - 2017 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6347 AB - Scaffolds used for osteochondral tissue engineering should comprise two distinct regions: a bottom region with characteristics corresponding to bone tissue, such as a porous structure with mineral components (predominantly hydroxyapatite), and a top region with characteristics of articular cartilage, which is gelatinous with high water content. In this work, we have investigated possibilities to formulate and optimize a procedure for obtaining such biphasic scaffolds based on gellan gum (GG). A porous base layer of the scaffold was obtained by lyophilization of the 2 % GG hydrogel with dispersed bioactive glass nanoparticles, as hydroxyapatite precursors. Next, different procedures were investigated to produce the upper GG hydrogel such as partial immersion of the porous layer in the GG solution and pouring the GG solution over the porous layer at different moisture conditions and temperatures. A simple mathematical model was derived and subsequently experimentally validated to find optimal temperatures of the porous layer, GG solution and the surrounding environment to provide adequate gelation rate to form the GG hydrogel on top of the porous layer with a thin interfacial zone. PB - Institute of Technical Sciences of SASA C3 - Seventieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts T1 - Development and optimization of the production procedure of biphasic scaffolds for osteochondral tissue engineering SP - 4 UR - https://hdl.handle.net/21.15107/rcub_technorep_6347 ER -
@conference{ author = "Radonjić, Mia and Zvicer, Jovana and Obradović, Bojana", year = "2017", abstract = "Scaffolds used for osteochondral tissue engineering should comprise two distinct regions: a bottom region with characteristics corresponding to bone tissue, such as a porous structure with mineral components (predominantly hydroxyapatite), and a top region with characteristics of articular cartilage, which is gelatinous with high water content. In this work, we have investigated possibilities to formulate and optimize a procedure for obtaining such biphasic scaffolds based on gellan gum (GG). A porous base layer of the scaffold was obtained by lyophilization of the 2 % GG hydrogel with dispersed bioactive glass nanoparticles, as hydroxyapatite precursors. Next, different procedures were investigated to produce the upper GG hydrogel such as partial immersion of the porous layer in the GG solution and pouring the GG solution over the porous layer at different moisture conditions and temperatures. A simple mathematical model was derived and subsequently experimentally validated to find optimal temperatures of the porous layer, GG solution and the surrounding environment to provide adequate gelation rate to form the GG hydrogel on top of the porous layer with a thin interfacial zone.", publisher = "Institute of Technical Sciences of SASA", journal = "Seventieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts", title = "Development and optimization of the production procedure of biphasic scaffolds for osteochondral tissue engineering", pages = "4", url = "https://hdl.handle.net/21.15107/rcub_technorep_6347" }
Radonjić, M., Zvicer, J.,& Obradović, B.. (2017). Development and optimization of the production procedure of biphasic scaffolds for osteochondral tissue engineering. in Seventieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts Institute of Technical Sciences of SASA., 4. https://hdl.handle.net/21.15107/rcub_technorep_6347
Radonjić M, Zvicer J, Obradović B. Development and optimization of the production procedure of biphasic scaffolds for osteochondral tissue engineering. in Seventieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts. 2017;:4. https://hdl.handle.net/21.15107/rcub_technorep_6347 .
Radonjić, Mia, Zvicer, Jovana, Obradović, Bojana, "Development and optimization of the production procedure of biphasic scaffolds for osteochondral tissue engineering" in Seventieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts (2017):4, https://hdl.handle.net/21.15107/rcub_technorep_6347 .