Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials
Аутори
Vanheuverzwijn, JéromeMaillard, Eloise-Eliane
Mahat, Amal
Fowler, Lee
Monteyne, Daniel
Bonnaud, Leïla
Landercy, Nicolas
Hemberg, Axel
Janković, Ana
Meyer, Franck
Mišković-Stanković, Vesna
Stevanović, Milena
Mirica, Codruta
Pérez-Morga, David
Luginbuehl, Reto
Combes, Christèle
Furtos, Gabriel
Fontaine, Véronique
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Medical implants have improved the quality of life of many patients. However, surgical intervention may eventually lead to implant microbial contamination. The aims of this research were to develop an easy, robust, quantitative assay to assess surface antimicrobial activities, especially the anti-nascent biofilm activity, and to identify control surfaces, allowing for international comparisons. Using new antimicrobial assays to assess the inhibition of nascent biofilm during persistent contact or after transient contact with bacteria, we show that the 5 cent Euro coin or other metal-based antibacterial coins can be used as positive controls, as more than 4 log reduction on bacterial survival was observed when using either S. aureus or P. aeruginosa as targets. The methods and controls described here could be useful to develop an easy, flexible and standardizable assay to assess relevant antimicrobial activities of new implant materials developed by industries and academics.
Кључне речи:
anti-biofilm / copper / infection / medical device / metal ion / surfaceИзвор:
Microorganisms, 2023, 11, 4, 1023-Издавач:
- MDPI
Финансирање / пројекти:
- This research project has been initiated under the iPROMEDAI COST action TD1305.
- EEuropean Union’s Horizon 2020 research and innovation program under grant agreement No. 952033.
- University Union—Nikola Tesla, Belgrade, Serbia
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Vanheuverzwijn, Jérome AU - Maillard, Eloise-Eliane AU - Mahat, Amal AU - Fowler, Lee AU - Monteyne, Daniel AU - Bonnaud, Leïla AU - Landercy, Nicolas AU - Hemberg, Axel AU - Janković, Ana AU - Meyer, Franck AU - Mišković-Stanković, Vesna AU - Stevanović, Milena AU - Mirica, Codruta AU - Pérez-Morga, David AU - Luginbuehl, Reto AU - Combes, Christèle AU - Furtos, Gabriel AU - Fontaine, Véronique PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6381 AB - Medical implants have improved the quality of life of many patients. However, surgical intervention may eventually lead to implant microbial contamination. The aims of this research were to develop an easy, robust, quantitative assay to assess surface antimicrobial activities, especially the anti-nascent biofilm activity, and to identify control surfaces, allowing for international comparisons. Using new antimicrobial assays to assess the inhibition of nascent biofilm during persistent contact or after transient contact with bacteria, we show that the 5 cent Euro coin or other metal-based antibacterial coins can be used as positive controls, as more than 4 log reduction on bacterial survival was observed when using either S. aureus or P. aeruginosa as targets. The methods and controls described here could be useful to develop an easy, flexible and standardizable assay to assess relevant antimicrobial activities of new implant materials developed by industries and academics. PB - MDPI T2 - Microorganisms T1 - Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials IS - 4 SP - 1023 VL - 11 DO - 10.3390/microorganisms11041023 ER -
@article{ author = "Vanheuverzwijn, Jérome and Maillard, Eloise-Eliane and Mahat, Amal and Fowler, Lee and Monteyne, Daniel and Bonnaud, Leïla and Landercy, Nicolas and Hemberg, Axel and Janković, Ana and Meyer, Franck and Mišković-Stanković, Vesna and Stevanović, Milena and Mirica, Codruta and Pérez-Morga, David and Luginbuehl, Reto and Combes, Christèle and Furtos, Gabriel and Fontaine, Véronique", year = "2023", abstract = "Medical implants have improved the quality of life of many patients. However, surgical intervention may eventually lead to implant microbial contamination. The aims of this research were to develop an easy, robust, quantitative assay to assess surface antimicrobial activities, especially the anti-nascent biofilm activity, and to identify control surfaces, allowing for international comparisons. Using new antimicrobial assays to assess the inhibition of nascent biofilm during persistent contact or after transient contact with bacteria, we show that the 5 cent Euro coin or other metal-based antibacterial coins can be used as positive controls, as more than 4 log reduction on bacterial survival was observed when using either S. aureus or P. aeruginosa as targets. The methods and controls described here could be useful to develop an easy, flexible and standardizable assay to assess relevant antimicrobial activities of new implant materials developed by industries and academics.", publisher = "MDPI", journal = "Microorganisms", title = "Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials", number = "4", pages = "1023", volume = "11", doi = "10.3390/microorganisms11041023" }
Vanheuverzwijn, J., Maillard, E., Mahat, A., Fowler, L., Monteyne, D., Bonnaud, L., Landercy, N., Hemberg, A., Janković, A., Meyer, F., Mišković-Stanković, V., Stevanović, M., Mirica, C., Pérez-Morga, D., Luginbuehl, R., Combes, C., Furtos, G.,& Fontaine, V.. (2023). Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials. in Microorganisms MDPI., 11(4), 1023. https://doi.org/10.3390/microorganisms11041023
Vanheuverzwijn J, Maillard E, Mahat A, Fowler L, Monteyne D, Bonnaud L, Landercy N, Hemberg A, Janković A, Meyer F, Mišković-Stanković V, Stevanović M, Mirica C, Pérez-Morga D, Luginbuehl R, Combes C, Furtos G, Fontaine V. Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials. in Microorganisms. 2023;11(4):1023. doi:10.3390/microorganisms11041023 .
Vanheuverzwijn, Jérome, Maillard, Eloise-Eliane, Mahat, Amal, Fowler, Lee, Monteyne, Daniel, Bonnaud, Leïla, Landercy, Nicolas, Hemberg, Axel, Janković, Ana, Meyer, Franck, Mišković-Stanković, Vesna, Stevanović, Milena, Mirica, Codruta, Pérez-Morga, David, Luginbuehl, Reto, Combes, Christèle, Furtos, Gabriel, Fontaine, Véronique, "Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials" in Microorganisms, 11, no. 4 (2023):1023, https://doi.org/10.3390/microorganisms11041023 . .