Uticaj holesterola na fluidnost membrane lipozoma, veličinu lipozomnih čestica i njihov zeta potencijal
Само за регистроване кориснике
2017
Аутори
Jovanović, Aleksandra A.Balanč, Bojana
Pravilović, Radoslava
Ota, Ajda
Poklar-Ulrih, Nataša
Nedović, Viktor
Bugarski, Branko
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Cilj ove studije bilo je ispitivanje uticaja različitih sadržaja holesterola (0-
50 mol %) na fluidnost membrana, veličinu čestica, polidisperzni indeks
(PDI) i zeta potencijal lipozoma, pripremljenih metodom tankog filma i
upotrebom fosfatidilholina soje, Phospholipon 90 (Ph). Merenjem
polarizacije (p) i anizotropije (r), u temperaturnom intervalu od 20 do 60˚C,
upotrebom fluorofore 1,6-difenil-1,3,5-heksatrien (DPH), pokazano je da
povećanjem koncentracije holesterola u lipozomima dolazi do smanjenja
fluidnosti i povećanja rigidnosti membrane lipozoma. Foton korelaciona
spektroskopija je kod malih unilamelarnih lipozoma (SUV) pokazala
kontinuirano povećanje veličine čestica sa porastom koncentracije
holesterola, dok kod multilamelarnih lipozoma (MLV) trend kontinuiranog
rasta čestica nije primećen. U oba slučaja, statistički značajno veće čestice
su dobijene sa najvišom koncentracijom holesterola (50 mol %). Prema
dobijenim vrednostima PDI, samo SUV bez holesterola i sa ...5, 30 i 40 mol %
holesterola, poseduju usku raspodelu čestica po veličini (PDI manji od 0,2).
Vrednosti zeta potencijala, kao važnog parametra stabilnosti sistema, bile su
negativne u svim uzorcima, dok su najviše apsolutne vrednosti dostignute sa
50 mol % holesterola. Optimalna koncentracija holesterola za dobijanje
lipozoma odgovarajuće stabilnosti i fluidnosti membrana jeste 50 mol %.
The aim of this study was examination of the influence of various cholesterol
content (0-50 mol %) on membrane fluidity, vesicle size, polydispersity index
(PDI) and zeta potential of liposomes prepared using the thin film method
and phosphatidylcholine from soybean, Phospholipon 90 (Ph). Polarization
(p) and anisotropy (r) measurements, within the temperature range of 20 to
60˚C, using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH), have
shown that increasing the cholesterol content in the liposomes decreased the
fluidity and enhanced the rigidity of liposomal membranes. The photon
correlation spectroscopy data have shown that the size of small unilamellar
vesicles (SUV) increased continuously with the increase in cholesterol
concentration, while multilamellar vesicles (MLV) did not show similar
trend. In both cases, significantly larger vesicles were obtained using the
highest content of cholesterol (50 mol %). According to the PDI values, only
SUV without cho...lesterol and with 5, 30 and 40 mol % of cholesterol exhibit
narrow size distribution (PDI < 0,2). Furthermore, values of zeta potential,
as an important parameter of system stability, were negative in all samples,
while the highest absolute values were achieved with 50 mol % of cholesterol. The optimal concentration of cholesterol to obtain stabile
liposomes and an appropriate fluidity of the membrane has been 50 mol %.
Кључне речи:
lipozomi / holesterol / polarizacija / anizotropija / veličina čestica / zeta potencijal liposomes / cholesterol / polarization / anisotropy / vesicle size / zeta potentialИзвор:
V International Congress: „Engineering, Environment and Materials in Processing Industry“, 2017, 1502-1514Издавач:
- Faculty of Technology, Zvornik
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
Институција/група
Inovacioni centarTY - CONF AU - Jovanović, Aleksandra A. AU - Balanč, Bojana AU - Pravilović, Radoslava AU - Ota, Ajda AU - Poklar-Ulrih, Nataša AU - Nedović, Viktor AU - Bugarski, Branko PY - 2017 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6447 AB - Cilj ove studije bilo je ispitivanje uticaja različitih sadržaja holesterola (0- 50 mol %) na fluidnost membrana, veličinu čestica, polidisperzni indeks (PDI) i zeta potencijal lipozoma, pripremljenih metodom tankog filma i upotrebom fosfatidilholina soje, Phospholipon 90 (Ph). Merenjem polarizacije (p) i anizotropije (r), u temperaturnom intervalu od 20 do 60˚C, upotrebom fluorofore 1,6-difenil-1,3,5-heksatrien (DPH), pokazano je da povećanjem koncentracije holesterola u lipozomima dolazi do smanjenja fluidnosti i povećanja rigidnosti membrane lipozoma. Foton korelaciona spektroskopija je kod malih unilamelarnih lipozoma (SUV) pokazala kontinuirano povećanje veličine čestica sa porastom koncentracije holesterola, dok kod multilamelarnih lipozoma (MLV) trend kontinuiranog rasta čestica nije primećen. U oba slučaja, statistički značajno veće čestice su dobijene sa najvišom koncentracijom holesterola (50 mol %). Prema dobijenim vrednostima PDI, samo SUV bez holesterola i sa 5, 30 i 40 mol % holesterola, poseduju usku raspodelu čestica po veličini (PDI manji od 0,2). Vrednosti zeta potencijala, kao važnog parametra stabilnosti sistema, bile su negativne u svim uzorcima, dok su najviše apsolutne vrednosti dostignute sa 50 mol % holesterola. Optimalna koncentracija holesterola za dobijanje lipozoma odgovarajuće stabilnosti i fluidnosti membrana jeste 50 mol %. AB - The aim of this study was examination of the influence of various cholesterol content (0-50 mol %) on membrane fluidity, vesicle size, polydispersity index (PDI) and zeta potential of liposomes prepared using the thin film method and phosphatidylcholine from soybean, Phospholipon 90 (Ph). Polarization (p) and anisotropy (r) measurements, within the temperature range of 20 to 60˚C, using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH), have shown that increasing the cholesterol content in the liposomes decreased the fluidity and enhanced the rigidity of liposomal membranes. The photon correlation spectroscopy data have shown that the size of small unilamellar vesicles (SUV) increased continuously with the increase in cholesterol concentration, while multilamellar vesicles (MLV) did not show similar trend. In both cases, significantly larger vesicles were obtained using the highest content of cholesterol (50 mol %). According to the PDI values, only SUV without cholesterol and with 5, 30 and 40 mol % of cholesterol exhibit narrow size distribution (PDI < 0,2). Furthermore, values of zeta potential, as an important parameter of system stability, were negative in all samples, while the highest absolute values were achieved with 50 mol % of cholesterol. The optimal concentration of cholesterol to obtain stabile liposomes and an appropriate fluidity of the membrane has been 50 mol %. PB - Faculty of Technology, Zvornik C3 - V International Congress: „Engineering, Environment and Materials in Processing Industry“ T1 - Uticaj holesterola na fluidnost membrane lipozoma, veličinu lipozomnih čestica i njihov zeta potencijal EP - 1514 SP - 1502 DO - 10.7251/EEMSR15011502J ER -
@conference{ author = "Jovanović, Aleksandra A. and Balanč, Bojana and Pravilović, Radoslava and Ota, Ajda and Poklar-Ulrih, Nataša and Nedović, Viktor and Bugarski, Branko", year = "2017", abstract = "Cilj ove studije bilo je ispitivanje uticaja različitih sadržaja holesterola (0- 50 mol %) na fluidnost membrana, veličinu čestica, polidisperzni indeks (PDI) i zeta potencijal lipozoma, pripremljenih metodom tankog filma i upotrebom fosfatidilholina soje, Phospholipon 90 (Ph). Merenjem polarizacije (p) i anizotropije (r), u temperaturnom intervalu od 20 do 60˚C, upotrebom fluorofore 1,6-difenil-1,3,5-heksatrien (DPH), pokazano je da povećanjem koncentracije holesterola u lipozomima dolazi do smanjenja fluidnosti i povećanja rigidnosti membrane lipozoma. Foton korelaciona spektroskopija je kod malih unilamelarnih lipozoma (SUV) pokazala kontinuirano povećanje veličine čestica sa porastom koncentracije holesterola, dok kod multilamelarnih lipozoma (MLV) trend kontinuiranog rasta čestica nije primećen. U oba slučaja, statistički značajno veće čestice su dobijene sa najvišom koncentracijom holesterola (50 mol %). Prema dobijenim vrednostima PDI, samo SUV bez holesterola i sa 5, 30 i 40 mol % holesterola, poseduju usku raspodelu čestica po veličini (PDI manji od 0,2). Vrednosti zeta potencijala, kao važnog parametra stabilnosti sistema, bile su negativne u svim uzorcima, dok su najviše apsolutne vrednosti dostignute sa 50 mol % holesterola. Optimalna koncentracija holesterola za dobijanje lipozoma odgovarajuće stabilnosti i fluidnosti membrana jeste 50 mol %., The aim of this study was examination of the influence of various cholesterol content (0-50 mol %) on membrane fluidity, vesicle size, polydispersity index (PDI) and zeta potential of liposomes prepared using the thin film method and phosphatidylcholine from soybean, Phospholipon 90 (Ph). Polarization (p) and anisotropy (r) measurements, within the temperature range of 20 to 60˚C, using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH), have shown that increasing the cholesterol content in the liposomes decreased the fluidity and enhanced the rigidity of liposomal membranes. The photon correlation spectroscopy data have shown that the size of small unilamellar vesicles (SUV) increased continuously with the increase in cholesterol concentration, while multilamellar vesicles (MLV) did not show similar trend. In both cases, significantly larger vesicles were obtained using the highest content of cholesterol (50 mol %). According to the PDI values, only SUV without cholesterol and with 5, 30 and 40 mol % of cholesterol exhibit narrow size distribution (PDI < 0,2). Furthermore, values of zeta potential, as an important parameter of system stability, were negative in all samples, while the highest absolute values were achieved with 50 mol % of cholesterol. The optimal concentration of cholesterol to obtain stabile liposomes and an appropriate fluidity of the membrane has been 50 mol %.", publisher = "Faculty of Technology, Zvornik", journal = "V International Congress: „Engineering, Environment and Materials in Processing Industry“", title = "Uticaj holesterola na fluidnost membrane lipozoma, veličinu lipozomnih čestica i njihov zeta potencijal", pages = "1514-1502", doi = "10.7251/EEMSR15011502J" }
Jovanović, A. A., Balanč, B., Pravilović, R., Ota, A., Poklar-Ulrih, N., Nedović, V.,& Bugarski, B.. (2017). Uticaj holesterola na fluidnost membrane lipozoma, veličinu lipozomnih čestica i njihov zeta potencijal. in V International Congress: „Engineering, Environment and Materials in Processing Industry“ Faculty of Technology, Zvornik., 1502-1514. https://doi.org/10.7251/EEMSR15011502J
Jovanović AA, Balanč B, Pravilović R, Ota A, Poklar-Ulrih N, Nedović V, Bugarski B. Uticaj holesterola na fluidnost membrane lipozoma, veličinu lipozomnih čestica i njihov zeta potencijal. in V International Congress: „Engineering, Environment and Materials in Processing Industry“. 2017;:1502-1514. doi:10.7251/EEMSR15011502J .
Jovanović, Aleksandra A., Balanč, Bojana, Pravilović, Radoslava, Ota, Ajda, Poklar-Ulrih, Nataša, Nedović, Viktor, Bugarski, Branko, "Uticaj holesterola na fluidnost membrane lipozoma, veličinu lipozomnih čestica i njihov zeta potencijal" in V International Congress: „Engineering, Environment and Materials in Processing Industry“ (2017):1502-1514, https://doi.org/10.7251/EEMSR15011502J . .