Приказ основних података о документу
Antioksidativna aktivnost etanolnih ekstrakata Thymus serpyllum
dc.creator | Jovanović, Aleksandra A. | |
dc.creator | Zdunić, Gordana | |
dc.creator | Ćujić, Nada | |
dc.creator | Šavikin, Katarina | |
dc.creator | Bukara, Katarina | |
dc.creator | Lević, Steva | |
dc.creator | Bugarski, Branko | |
dc.date.accessioned | 2023-06-02T10:22:10Z | |
dc.date.available | 2023-06-02T10:22:10Z | |
dc.date.issued | 2015 | |
dc.identifier.isbn | 978-99955-81-18-3 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6449 | |
dc.description.abstract | Wild Thyme (Thymus serpyllum, Lamiaceae) is a subshrub from the Lamiaceae family, with plants that are rich in essential oils and antioxidative polyphenolic substances. Reducing potential of phenolic compounds gives them a special role in the adsorption and neutralization of free radicals. In the present study, we used a method of maceration, lasting 15, 30, 60 and 90 minutes, degree of fragmentation 0.7, two types of solvent (50% and 96% ethanol) and ratio drugs:solvent 1:20. In Folin-Ciocalteu method, the higest total polyphenols content was obtained in 50% ethanolic extract after 90 minutes of extraction, while the lowest total polyphenols content was recorded in 96% ethanolic extract after 15 minutes (1173,21 mg GA/g DW and 102,56 mg GA/g DW, respectively). Ethanolic extracts were analized for antioxidant activity by using spectrophotometric methods, DPPH method and ABTS method. There is signifficant difference between antioxidant activity in 50% and in 96% ethanolic extracts. The worse result was obtained with 96% ethanol as extractant after 15 minutes of maceration (IC50 31,323 mg/ml and 0,116 mM Trolox/g DW). This study found that both total polyphenols and antioxidant activities determined were significantly affected by the type of solvent and time of extraction. Also, the levels of total soluble phenolic compounds were positively correlated with free radical scavenging activities against DPPH and ABTS radicals. | sr |
dc.description.abstract | Majčina dušica (Thymus serpyllum, Lamiaceae) je višegodišnji busen iz familije Lamiaceae, koju čine biljke bogate etarskim uljima i antioksidativnim polifenolnim supstancama. Antioksidativnu aktivnost fenolne komponente duguju svom redukujućem potencijalu, koji im daje posebnu ulogu u adsorpciji i neutralizaciji slobodnih radikala. U ovoj studiji ekstrakti T. serpyllum su dobijeni metodom maceracije, u trajanju od 15, 30, 60 i 90 minuta, korišćenjem droge stepena usitnjenosti 0,7, dva rastvarača (50% i 96% etanola) i odnosa droga:rastvarač 1:20. FolinCiocalteu metodom je određen sadržaj ukupnih polifenola, gde je 50% etanolni ekstrakt, nakon 90 minuta ekstrakcije sadržao najveću količinu ukupnih polifenola, dok su najniže vrednosti zabeležene kod 96% etanolnog ekstrakta, nakon 15 minuta (1173,21 mg GA/g suve droge, odnosno 102,56 mg GA/g suve droge). Primenom spektrofotometrijskih metoda, DPPH metode i ABTS metode, određena je antioksidativna aktivnost 50% i 96% etanolnih ekstrakata T. serpyllum. Statistika je pokazala da postoji značajna razlika između antioksidativnog kapaciteta 50% i 96% etanolnih ekstrakata. Najlošiju antioksidativnu aktivnost poseduje 96% etanolni ekstrakt, nakon 15 minuta ekstrakcije (IC50 31,323 mg/ml i 116 mM Troloxa/g suve droge). Sadržaj ukupnih fenola i antioksidativna aktivnost ekstrakata zavise od tipa rastvarača korišćenog za ekstrakciju i vremena ekstrakcije. Ovaj rad pokazuje da postoji pozitivna korelacija između sadržaja ukupnih polifenola i kapaciteta neutralizacije DPPH i ABTS radikala. | sr |
dc.language.iso | sr | sr |
dc.publisher | Faculty of Technology, Zvornik | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46013/RS// | sr |
dc.rights | restrictedAccess | sr |
dc.source | IV International Congress of Engineering, Environment and Materials in Processing Industry | sr |
dc.subject | Thymus serpyllum | sr |
dc.subject | total polyphenols | sr |
dc.subject | antioxidant activity | sr |
dc.subject | ethanolic extract | sr |
dc.subject | DPPH | sr |
dc.subject | ABTS | sr |
dc.subject | ukupni polifenoli | sr |
dc.subject | antioksidativna aktivnost | sr |
dc.subject | etanolni ekstrakt | sr |
dc.title | Antioksidativna aktivnost etanolnih ekstrakata Thymus serpyllum | sr |
dc.type | conferenceObject | sr |
dc.rights.license | ARR | sr |
dc.citation.epage | 452 | |
dc.citation.spage | 444 | |
dc.identifier.doi | 10.7251/EEMSR1501444J | |
dc.type.version | publishedVersion | sr |