Ag+-DOPED HYDROXYAPATITE: CELL PARAMETERS, MORPHOLOGY, THERMAL AND SPECTRAL PROPERTIES

Abstract

Synthetic hydroxyapatite (HAp) [Ca10(PO4)6(OH)2] is very similar to the inorganic part of bones and the dentine of teeth. Consequently, it has well known biocompatibility and bioactivity, and it is nontoxic in any quantity and osteoconductive. HAp is used as a powder and as a coating for metal implants, compact and scaffolds [1,2]. However, during incorporation of filler HAp or HAp-covered implants in the living body, infection by pathogenic microorganisms can appear. To prevent this situation and the need for a second operation, the implants are treated by biocidal metal ions or antibiotics. Use of antibiotics is not suitable because their effect is not long-lasting and microorganisms can develop resistance. HAp powders doped with Ag+ (0.2 and 0.4 mol % as compared to Ca) were synthesized by a hydrothermal method in order to obtain biomaterial with an antimicrobial effect [3]. Obtained powders were analysed by field emission scanning electron microscopy, X-ray diffraction, atomic absorption and energy-dispersive X-Ray spectroscopy. Also, the antimicrobial activity of Ag+ -doped HAp against different pathogens: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans was evaluated in vitro. The results showed that the obtained powders had good antimicrobial activity. As a supplement to characterization of Ag+ doped HAppowders, in the present study, the following data were presented: cell parameters, which are determined by Jade 5 software starting with data from PDF card 09-0482 for refinement, thermal analysis curves and vibrational spectra obtained by fourier transform infrared spectroscopy

Sintetički hidroksiapatit (HAp) [Ca10(PO4)6(OH)2] je veoma sličan neorganskom delu kosti i dentina zuba. HAp je poznat kao materijal dobre biokompatibilnosti i bioaktivnosti, netoksičan je u bilo kojoj količini i osteokonduktivan. HAp se koristi kao prah, za naparavanje metalnih implanata, kao kompakt i kao skafold [1,2]. Međutim, tokom hirurške intervencije i ugradnje HAp-a kao punioca ili HAp-om prekrivenih implanata u tela, može se razviti infekcija patogenim mikroorganizmima. Da bi se predupredila takva situacija i potreba za drugom operacijom, implantni materijal se tretira biocidnim jonima metala ili antibioticima. Korišćenje antibiotika nije pogodno jer se pokazalo da njihovo dejstvo nije dugoročno i mikroorganizmi mogu razviti otpornost. Prahovi HAp-a dopirani sa Ag+ (0,2 i 0,4 mol % u odnosu na Ca) su sintetisani hidrotermalnom metodom u cilju dobijanja biomaterijala sa antimikrobnim dejstvom [3]. Dobijeni prahovi su analizirani visoko rezolucionim skenirajućim elektronskim mikroskopom, rendgenskom difrakcionom analizom, atomskom apsorpcionom i energetskom disperzionom rendgenskom spektroskopijom. Takođe, antimikrobna aktivnost Ag+ -dopiranih HAp-a protiv različitih patogena: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa i Candida albicans ispitana je in vitro. Rezultati su pokazali da dobijeni prahovi imaju dobru antimikrobnu aktivnost. Kao dodatak karakterizaciji Ag+ -dopiranih HAp prahova, u ovoj studiji, sledeći podaci su prezentovani: parametri jedinične ćelije, koji su određeni upotrebom programa Jade 5 polazeći od podataka iz kartice PDF 09-0482 za utačnjavanje, krive dobijene termalnom analizom i infracrveni vibracioni spektri.