Promising Hydroxyapatite/Nifuroksazid Conjugate

Abstract

The synergistic effect of two materials is a desirable phenomenon when designing a conjugate material. Synthetic Hydroxyapatite (HAp, Ca10(PO4)6(OH)2) is very similar to the inorganic part of bones and teeth of mammals. It is a well-known biomaterial with good biocompatibility, osteoconductivity and bioactivity. Nifuroxazide (NFX, C12H9N3O5) is a broad-spectrum antibacterial drug and poorly soluble in water. Nano HAp and NFX were mixed and homogenized in rotovapor giving as a result HAp/NFX conjugate. All materials were analyzed by Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (FESEM), X-Ray diffraction (XRD), Thermogravimetry (TG) and Brunauer–Emmett–Teller (BET) method. The in vitro antibacterial activities of HAp, NFX and HAp/NFX were examined against Gram-positive bacteria Staphylococcus aureus, Gram-negative bacteria Escherichia coli and yeast Candida albicans. In vitro drug release in simulated stomach acid and intestinal fluid showed a much faster release of NFX from HAp surface than those of raw drug. Afore mentioned analyses showed the unique properties of the HAp/NFX conjugate and introduced it as particularly prominent drug carrier.