TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Đorđević, Verica
AU  - Petrović, Predrag
AU  - Pljevljakusic, Dejan S.
AU  - Zdunic, Gordana M.
AU  - Savikin, Katarina P.
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4794
AB  - The aims of the presented study were the investigation of the effect of different ultrasound-assisted extraction conditions (particle size, solid-to-solvent ratio, ethanol concentration and time) on polyphenol and flavonoid contents and antioxidant activity of wild thyme (Thymus serpyllum L.) extracts, HPLC qualitative and quantitative analysis, as well as the examination of antimicrobial properties against seven bacterial strains and one fungal strain. According to the results, the highest total polyphenol and flavonoid contents were reached with particle size of 0.3 mm, 1:30 solid-to-solvent ratio, 30 % ethanol and 30 min of extraction (31.9 +/- 0.1 mg gallic acid equivalents (GAE)/L and 16.8 +/- 0.2 mg catechin equivalents (CE)/L, respectively). The most abundant polyphenolic compounds were rosmarinic acid, luteolin 7-O-glucumnide and salvianolic acid I (185.2 +/- 7.3, 141.6 +/- 3.4 and 76.4 +/- 2.1 mu g/mL, respectively). The best antioxidant recovery was achieved with particles of 0.3 mm and 1:30 solid-to-solvent ratio. Furthermore, the addition of ethanol in aqueous extraction medium has caused significantly better antioxidant activity than pure water. On the other hand, there was no statistically significant difference in antioxidant capacity between various ethanol percentage, as well as between time of 15 and 30 min. Selected extracts have shown significant antibacterial activity, particularly against Enterococcus faecalis, Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus and Yersinia enterocolitica.
T2  - Journal of Applied Research on Medicinal and Aromatic Plants
T1  - The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme
VL  - 25
DO  - 10.1016/j.jarmap.2021.100328
ER  - 

@article{
author = "Jovanović, Aleksandra and Đorđević, Verica and Petrović, Predrag and Pljevljakusic, Dejan S. and Zdunic, Gordana M. and Savikin, Katarina P. and Bugarski, Branko",
year = "2021",
abstract = "The aims of the presented study were the investigation of the effect of different ultrasound-assisted extraction conditions (particle size, solid-to-solvent ratio, ethanol concentration and time) on polyphenol and flavonoid contents and antioxidant activity of wild thyme (Thymus serpyllum L.) extracts, HPLC qualitative and quantitative analysis, as well as the examination of antimicrobial properties against seven bacterial strains and one fungal strain. According to the results, the highest total polyphenol and flavonoid contents were reached with particle size of 0.3 mm, 1:30 solid-to-solvent ratio, 30 % ethanol and 30 min of extraction (31.9 +/- 0.1 mg gallic acid equivalents (GAE)/L and 16.8 +/- 0.2 mg catechin equivalents (CE)/L, respectively). The most abundant polyphenolic compounds were rosmarinic acid, luteolin 7-O-glucumnide and salvianolic acid I (185.2 +/- 7.3, 141.6 +/- 3.4 and 76.4 +/- 2.1 mu g/mL, respectively). The best antioxidant recovery was achieved with particles of 0.3 mm and 1:30 solid-to-solvent ratio. Furthermore, the addition of ethanol in aqueous extraction medium has caused significantly better antioxidant activity than pure water. On the other hand, there was no statistically significant difference in antioxidant capacity between various ethanol percentage, as well as between time of 15 and 30 min. Selected extracts have shown significant antibacterial activity, particularly against Enterococcus faecalis, Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus and Yersinia enterocolitica.",
journal = "Journal of Applied Research on Medicinal and Aromatic Plants",
title = "The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme",
volume = "25",
doi = "10.1016/j.jarmap.2021.100328"
}

Jovanović, A., Đorđević, V., Petrović, P., Pljevljakusic, D. S., Zdunic, G. M., Savikin, K. P.,& Bugarski, B.. (2021). The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme. in Journal of Applied Research on Medicinal and Aromatic Plants, 25.
https://doi.org/10.1016/j.jarmap.2021.100328

Jovanović A, Đorđević V, Petrović P, Pljevljakusic DS, Zdunic GM, Savikin KP, Bugarski B. The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme. in Journal of Applied Research on Medicinal and Aromatic Plants. 2021;25.
doi:10.1016/j.jarmap.2021.100328 .

Jovanović, Aleksandra, Đorđević, Verica, Petrović, Predrag, Pljevljakusic, Dejan S., Zdunic, Gordana M., Savikin, Katarina P., Bugarski, Branko, "The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme" in Journal of Applied Research on Medicinal and Aromatic Plants, 25 (2021),
https://doi.org/10.1016/j.jarmap.2021.100328 . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Petrović, Predrag
AU  - Zdunic, Gordana M.
AU  - Savikin, Katarina P.
AU  - Kitic, Dusanka
AU  - Đorđević, Verica
AU  - Bugarski, Branko
AU  - Brankovic, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4798
AB  - In the presented study, polyphenol profile, spasmolytic, antimicrobial and antioxidant properties of lyophilized Thymus serpyllum extracts were examined. The main phenolic acid in the extracts was rosmarinic acid, followed by salvianolic acid I, salvianolic acid K isomer and caffeic acid, whereas chlorogenic acid was detected only in traces; the dominant flavonoids were luteolin 7-O-glucuronide and 6-hydroxyluteolin 7-O-glucoside, while the contents of apigenin glucuronide and 6,8-di-C-glucosylapigenin were significantly lower. All tested extracts have shown spasmolytic effect in a dose-dependent manner on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions; lyophilized extract obtained in maceration has shown significantly better spasmolytic activity. The mentioned extract has shown dosedependent spasmolytic effect in the model of contractions induced by barium chloride and calcium chloride, as well. Furthermore, lyophilized extracts were effective against Gram-positive bacteria and showed only moderate antifungal activity. Ultrasound-assisted extraction provided extracts with better ability of neutralization DPPH radicals, whereas maceration and ultrasound-assisted extraction approved to be better regarding beta-carotene bleaching assay. Due to spasmolytic, antimicrobial and antioxidant properties shown in the present study, polyphenols-rich T. serpyllum extract may be used as adjuvant in therapy of gastrointestinal disorders and infections caused by some of the susceptible pathogens.
T2  - South African Journal of Botany
T1  - Influence of lyophilized Thymus serpyllum L. extracts on the gastrointestinal system: Spasmolytic, antimicrobial and antioxidant properties
EP  - 283
SP  - 274
VL  - 142
DO  - 10.1016/j.sajb.2021.06.028
ER  - 

@article{
author = "Jovanović, Aleksandra and Petrović, Predrag and Zdunic, Gordana M. and Savikin, Katarina P. and Kitic, Dusanka and Đorđević, Verica and Bugarski, Branko and Brankovic, Suzana",
year = "2021",
abstract = "In the presented study, polyphenol profile, spasmolytic, antimicrobial and antioxidant properties of lyophilized Thymus serpyllum extracts were examined. The main phenolic acid in the extracts was rosmarinic acid, followed by salvianolic acid I, salvianolic acid K isomer and caffeic acid, whereas chlorogenic acid was detected only in traces; the dominant flavonoids were luteolin 7-O-glucuronide and 6-hydroxyluteolin 7-O-glucoside, while the contents of apigenin glucuronide and 6,8-di-C-glucosylapigenin were significantly lower. All tested extracts have shown spasmolytic effect in a dose-dependent manner on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions; lyophilized extract obtained in maceration has shown significantly better spasmolytic activity. The mentioned extract has shown dosedependent spasmolytic effect in the model of contractions induced by barium chloride and calcium chloride, as well. Furthermore, lyophilized extracts were effective against Gram-positive bacteria and showed only moderate antifungal activity. Ultrasound-assisted extraction provided extracts with better ability of neutralization DPPH radicals, whereas maceration and ultrasound-assisted extraction approved to be better regarding beta-carotene bleaching assay. Due to spasmolytic, antimicrobial and antioxidant properties shown in the present study, polyphenols-rich T. serpyllum extract may be used as adjuvant in therapy of gastrointestinal disorders and infections caused by some of the susceptible pathogens.",
journal = "South African Journal of Botany",
title = "Influence of lyophilized Thymus serpyllum L. extracts on the gastrointestinal system: Spasmolytic, antimicrobial and antioxidant properties",
pages = "283-274",
volume = "142",
doi = "10.1016/j.sajb.2021.06.028"
}

Jovanović, A., Petrović, P., Zdunic, G. M., Savikin, K. P., Kitic, D., Đorđević, V., Bugarski, B.,& Brankovic, S.. (2021). Influence of lyophilized Thymus serpyllum L. extracts on the gastrointestinal system: Spasmolytic, antimicrobial and antioxidant properties. in South African Journal of Botany, 142, 274-283.
https://doi.org/10.1016/j.sajb.2021.06.028

Jovanović A, Petrović P, Zdunic GM, Savikin KP, Kitic D, Đorđević V, Bugarski B, Brankovic S. Influence of lyophilized Thymus serpyllum L. extracts on the gastrointestinal system: Spasmolytic, antimicrobial and antioxidant properties. in South African Journal of Botany. 2021;142:274-283.
doi:10.1016/j.sajb.2021.06.028 .

Jovanović, Aleksandra, Petrović, Predrag, Zdunic, Gordana M., Savikin, Katarina P., Kitic, Dusanka, Đorđević, Verica, Bugarski, Branko, Brankovic, Suzana, "Influence of lyophilized Thymus serpyllum L. extracts on the gastrointestinal system: Spasmolytic, antimicrobial and antioxidant properties" in South African Journal of Botany, 142 (2021):274-283,
https://doi.org/10.1016/j.sajb.2021.06.028 . .