@conference{
author = "Banićević, Ivana and Radonjić, Mia and Pavlović, Marija and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2021",
abstract = "Osteosarcoma is the most common type of bone cancer, which affects both children and
adults. Treatment of osteosarcoma exhibits slow progress due to inadequacy of both in vivo
animal models and 2D in vitro models regularly used for antitumor drug testing. Our
approach is to create a physiologically relevant 3D in vitro model for osteosarcoma cell
cultivation, which has the potential to overcome inherent weaknesses of 2D in vitro and
animal models. In order to imitate native osteosarcoma microenvironment, macroporous
alginate scaffolds with incorporated hydroxyapatite/β-tricalcium phosphate (HAp/β-TCP)
powder were produced with two compositions: 1 wt% alginate, 1 wt% powder and 2 wt.%
alginate, 2 wt% powder. Bioactivity and stability of the scaffolds were investigated under
biomimetic conditions of continuous flow of the culture medium in perfusion bioreactor at
the superficial medium velocity of 400 µm/s, which was reported in literature to be
beneficial for osteogenesis. Scaffolds with the higher alginate concentration was shown to be
more stable in the culture medium, since the scaffolds with the lower alginate concentration
disintegrated after 5-7 days under flow conditions. Biocompatibility of the obtained scaffolds
was investigated in short-term cultivation studies of murine osteosarcoma cells K7M2-wt
seeded onto the scaffolds. The scaffolds were cultivated in perfusion bioreactors at the
superficial flow velocity of 15 µm/s, while static cultures served as a control. After
cultivation, osteosarcoma cells remained adhered to the scaffold surface, expressed
metabolic activity and retained their initial proliferation ability while the flow was shown to
positively affect the cultivated cells.",
publisher = "Institute of Technical Sciences of SASA",
journal = "Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts",
title = "Development of a physiologically relevant 3D in vitro model for osteosarcoma cell cultivation comprising alginate composite scaffolds and a perfusion bioreactor system",
pages = "6",
volume = "17",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5028"
}
