TY  - CONF
AU  - Knežević-Jugović, Zorica
AU  - Jovanović, Jelena
AU  - Stefanović, Andrea
AU  - Žuža, Milena
AU  - Šekuljica, Nataša
AU  - Manojlović, Verica
AU  - Bugarski, Branko
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6263
AB  - The objective of this research was a production of egg white protein hydrolysates with improved antioxidant properties. For this purpose, a thermal and ultrasound pretreated egg white proteins were intensively hydrolysed with a commercial food-grade bacterial protease Alcalase. Thus obtained hydrolysates were further separated by sequential ultrafiltration into four peptide fraction viz. Fraction I (> 30kDa), II (10 - 30 kDa), III (1 - 10 kDa) and IV (< 1kDa) which were investigated in terms of their antioxidant activity. The antioxidant activity of hydrolysates and peptide fractions were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging assays and measuring ferric reducing antioxidant power assay. Scavenging of 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) by Fraction III, prepared with ultrasound pretreatment (35 kHz - 30 min) was found to be significantly higher than other fractions (21.17 ± 2.01 % and 80.72 ± 1.32 %, respectively), while ferric reducing antioxidant power assay has proven to be the best for thermal pretreatment. The results show that the fractionated hydrolysates were superior to the original hydrolysate in the antioxidative activity tested and can be concluded that by combining ultrasound pretreatment hydrolysates with improved functional and antioxidant properties can be produced enhancing utilization of egg white in food products.
PB  - Zvornik : Tehnološki fakultet
C3  - Proceedings / IV International Congress "Engineering, Ecology and Materials in the Processing Industry"
T1  - Аntioxidant activity of peptide fractions obtained by membrane ultrafiltration of egg white protein enzymatic hydrolysates
EP  - 286
SP  - 278
DO  - 10.7251/EEMEN1501278K
ER  - 

@conference{
author = "Knežević-Jugović, Zorica and Jovanović, Jelena and Stefanović, Andrea and Žuža, Milena and Šekuljica, Nataša and Manojlović, Verica and Bugarski, Branko",
year = "2015",
abstract = "The objective of this research was a production of egg white protein hydrolysates with improved antioxidant properties. For this purpose, a thermal and ultrasound pretreated egg white proteins were intensively hydrolysed with a commercial food-grade bacterial protease Alcalase. Thus obtained hydrolysates were further separated by sequential ultrafiltration into four peptide fraction viz. Fraction I (> 30kDa), II (10 - 30 kDa), III (1 - 10 kDa) and IV (< 1kDa) which were investigated in terms of their antioxidant activity. The antioxidant activity of hydrolysates and peptide fractions were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging assays and measuring ferric reducing antioxidant power assay. Scavenging of 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) by Fraction III, prepared with ultrasound pretreatment (35 kHz - 30 min) was found to be significantly higher than other fractions (21.17 ± 2.01 % and 80.72 ± 1.32 %, respectively), while ferric reducing antioxidant power assay has proven to be the best for thermal pretreatment. The results show that the fractionated hydrolysates were superior to the original hydrolysate in the antioxidative activity tested and can be concluded that by combining ultrasound pretreatment hydrolysates with improved functional and antioxidant properties can be produced enhancing utilization of egg white in food products.",
publisher = "Zvornik : Tehnološki fakultet",
journal = "Proceedings / IV International Congress "Engineering, Ecology and Materials in the Processing Industry"",
title = "Аntioxidant activity of peptide fractions obtained by membrane ultrafiltration of egg white protein enzymatic hydrolysates",
pages = "286-278",
doi = "10.7251/EEMEN1501278K"
}

Knežević-Jugović, Z., Jovanović, J., Stefanović, A., Žuža, M., Šekuljica, N., Manojlović, V.,& Bugarski, B.. (2015). Аntioxidant activity of peptide fractions obtained by membrane ultrafiltration of egg white protein enzymatic hydrolysates. in Proceedings / IV International Congress "Engineering, Ecology and Materials in the Processing Industry"
Zvornik : Tehnološki fakultet., 278-286.
https://doi.org/10.7251/EEMEN1501278K

Knežević-Jugović Z, Jovanović J, Stefanović A, Žuža M, Šekuljica N, Manojlović V, Bugarski B. Аntioxidant activity of peptide fractions obtained by membrane ultrafiltration of egg white protein enzymatic hydrolysates. in Proceedings / IV International Congress "Engineering, Ecology and Materials in the Processing Industry". 2015;:278-286.
doi:10.7251/EEMEN1501278K .

Knežević-Jugović, Zorica, Jovanović, Jelena, Stefanović, Andrea, Žuža, Milena, Šekuljica, Nataša, Manojlović, Verica, Bugarski, Branko, "Аntioxidant activity of peptide fractions obtained by membrane ultrafiltration of egg white protein enzymatic hydrolysates" in Proceedings / IV International Congress "Engineering, Ecology and Materials in the Processing Industry" (2015):278-286,
https://doi.org/10.7251/EEMEN1501278K . .