TY  - JOUR
AU  - Simović, Milica
AU  - Bezbradica, Dejan
AU  - Banjanac, Katarina
AU  - Milivojević, Ana
AU  - Fanuel, Mathieu
AU  - Rogniaux, Helene
AU  - Ropartz, David
AU  - Veličković, Dušan
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3258
AB  - Galacto-oligosaccharides (GOS) represent a diverse group of well-characterized prebiotic ingredients derived from lactose in a reaction catalyzed with beta-galactosidases. Enzymatic transgalactosylation results in a mixture of compounds of various degrees of polymerization and types of linkages. Because structure plays an important role in terms of prebiotic activity, it is of crucial importance to provide an insight into the mechanism of transgalactosylation reaction and occurrence of different types of beta-linkages during GOS synthesis. Our study proved that a novel one-step method, based on ion-mobility spectrometry tandem mass spectrometry (IMS-MS/MS), enables complete elucidation of GOS structure. It has been shown that) beta-galactosidase from Aspergillus oryzae has the highest affinity toward formation of beta-(1 - gt  3) or beta-(1 - gt  6) linkages. Additionally, it was observed that the occurrence of different linkages varies during the reaction course, indicating that tailoring favorable GOS structures with improved prebiotic activity can be achieved by adequate control of enzymatic synthesis.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Agricultural and Food Chemistry
T1  - Structural Elucidation of Enzymatically Synthesized Galacto-oligosaccharides Using Ion-Mobility Spectrometry Tandem Mass Spectrometry
EP  - 3615
IS  - 18
SP  - 3609
VL  - 64
DO  - 10.1021/acs.jafc.6b01293
ER  - 

@article{
author = "Simović, Milica and Bezbradica, Dejan and Banjanac, Katarina and Milivojević, Ana and Fanuel, Mathieu and Rogniaux, Helene and Ropartz, David and Veličković, Dušan",
year = "2016",
abstract = "Galacto-oligosaccharides (GOS) represent a diverse group of well-characterized prebiotic ingredients derived from lactose in a reaction catalyzed with beta-galactosidases. Enzymatic transgalactosylation results in a mixture of compounds of various degrees of polymerization and types of linkages. Because structure plays an important role in terms of prebiotic activity, it is of crucial importance to provide an insight into the mechanism of transgalactosylation reaction and occurrence of different types of beta-linkages during GOS synthesis. Our study proved that a novel one-step method, based on ion-mobility spectrometry tandem mass spectrometry (IMS-MS/MS), enables complete elucidation of GOS structure. It has been shown that) beta-galactosidase from Aspergillus oryzae has the highest affinity toward formation of beta-(1 - gt  3) or beta-(1 - gt  6) linkages. Additionally, it was observed that the occurrence of different linkages varies during the reaction course, indicating that tailoring favorable GOS structures with improved prebiotic activity can be achieved by adequate control of enzymatic synthesis.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural and Food Chemistry",
title = "Structural Elucidation of Enzymatically Synthesized Galacto-oligosaccharides Using Ion-Mobility Spectrometry Tandem Mass Spectrometry",
pages = "3615-3609",
number = "18",
volume = "64",
doi = "10.1021/acs.jafc.6b01293"
}

Simović, M., Bezbradica, D., Banjanac, K., Milivojević, A., Fanuel, M., Rogniaux, H., Ropartz, D.,& Veličković, D.. (2016). Structural Elucidation of Enzymatically Synthesized Galacto-oligosaccharides Using Ion-Mobility Spectrometry Tandem Mass Spectrometry. in Journal of Agricultural and Food Chemistry
Amer Chemical Soc, Washington., 64(18), 3609-3615.
https://doi.org/10.1021/acs.jafc.6b01293

Simović M, Bezbradica D, Banjanac K, Milivojević A, Fanuel M, Rogniaux H, Ropartz D, Veličković D. Structural Elucidation of Enzymatically Synthesized Galacto-oligosaccharides Using Ion-Mobility Spectrometry Tandem Mass Spectrometry. in Journal of Agricultural and Food Chemistry. 2016;64(18):3609-3615.
doi:10.1021/acs.jafc.6b01293 .

Simović, Milica, Bezbradica, Dejan, Banjanac, Katarina, Milivojević, Ana, Fanuel, Mathieu, Rogniaux, Helene, Ropartz, David, Veličković, Dušan, "Structural Elucidation of Enzymatically Synthesized Galacto-oligosaccharides Using Ion-Mobility Spectrometry Tandem Mass Spectrometry" in Journal of Agricultural and Food Chemistry, 64, no. 18 (2016):3609-3615,
https://doi.org/10.1021/acs.jafc.6b01293 . .