TY  - JOUR
AU  - Tadic, Vanja M.
AU  - Nesic, Ivana
AU  - Martinovic, Milica
AU  - Roj, Edward
AU  - Brasanac-Vukanovic, Snezana
AU  - Maksimović, Svetolik
AU  - Zugic, Ana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4907
AB  - Bilberry represents a valuable source of antioxidant substances responsible for its application for the treatment of different conditions (such as inflammation, cardiovascular disease, cancer, diabetes, and different age-related diseases) associated with increased oxidative stress. As oxidative stress might cause skin impairments, we aim to evaluate a topical preparation containing bilberry leaves extract and bilberry seeds oil, obtained as a byproduct of the food industry. To obtain the extracts, the conventional maceration technique for leaves, and supercritical carbon dioxide extraction for seeds were employed. The chemical profile of both actives was achieved by HPLC and GC methods, revealing the presence of phenolic acids (chlorogenic being the most abundant), flavonoids (isoquercetin in the highest amount), and resveratrol in leaves extract, while in seeds oil the essential omega-3 and omega-6 fatty acids were determined in favorable ratio, almost being 1. Antioxidant potential of the wild bilberry extract and seed oil was evaluated using in vitro DPPH and FRAP assays. Finally, effects of the oil-in-water creams with mentioned wild bilberry isolates on the skin were investigated in an in vivo study conducted on healthy human volunteers, revealing the significant beneficial effects when topically applied.
T2  - Antioxidants
T1  - Old Plant, New Possibilities: Wild Bilberry (Vaccinium myrtillus L., Ericaceae) in Topical Skin Preparation
IS  - 3
VL  - 10
DO  - 10.3390/antiox10030465
ER  - 

@article{
author = "Tadic, Vanja M. and Nesic, Ivana and Martinovic, Milica and Roj, Edward and Brasanac-Vukanovic, Snezana and Maksimović, Svetolik and Zugic, Ana",
year = "2021",
abstract = "Bilberry represents a valuable source of antioxidant substances responsible for its application for the treatment of different conditions (such as inflammation, cardiovascular disease, cancer, diabetes, and different age-related diseases) associated with increased oxidative stress. As oxidative stress might cause skin impairments, we aim to evaluate a topical preparation containing bilberry leaves extract and bilberry seeds oil, obtained as a byproduct of the food industry. To obtain the extracts, the conventional maceration technique for leaves, and supercritical carbon dioxide extraction for seeds were employed. The chemical profile of both actives was achieved by HPLC and GC methods, revealing the presence of phenolic acids (chlorogenic being the most abundant), flavonoids (isoquercetin in the highest amount), and resveratrol in leaves extract, while in seeds oil the essential omega-3 and omega-6 fatty acids were determined in favorable ratio, almost being 1. Antioxidant potential of the wild bilberry extract and seed oil was evaluated using in vitro DPPH and FRAP assays. Finally, effects of the oil-in-water creams with mentioned wild bilberry isolates on the skin were investigated in an in vivo study conducted on healthy human volunteers, revealing the significant beneficial effects when topically applied.",
journal = "Antioxidants",
title = "Old Plant, New Possibilities: Wild Bilberry (Vaccinium myrtillus L., Ericaceae) in Topical Skin Preparation",
number = "3",
volume = "10",
doi = "10.3390/antiox10030465"
}

Tadic, V. M., Nesic, I., Martinovic, M., Roj, E., Brasanac-Vukanovic, S., Maksimović, S.,& Zugic, A.. (2021). Old Plant, New Possibilities: Wild Bilberry (Vaccinium myrtillus L., Ericaceae) in Topical Skin Preparation. in Antioxidants, 10(3).
https://doi.org/10.3390/antiox10030465

Tadic VM, Nesic I, Martinovic M, Roj E, Brasanac-Vukanovic S, Maksimović S, Zugic A. Old Plant, New Possibilities: Wild Bilberry (Vaccinium myrtillus L., Ericaceae) in Topical Skin Preparation. in Antioxidants. 2021;10(3).
doi:10.3390/antiox10030465 .

Tadic, Vanja M., Nesic, Ivana, Martinovic, Milica, Roj, Edward, Brasanac-Vukanovic, Snezana, Maksimović, Svetolik, Zugic, Ana, "Old Plant, New Possibilities: Wild Bilberry (Vaccinium myrtillus L., Ericaceae) in Topical Skin Preparation" in Antioxidants, 10, no. 3 (2021),
https://doi.org/10.3390/antiox10030465 . .