TY  - JOUR
AU  - Rajilić-Stojanović, Mirjana
AU  - Heilig, Hans G. H. J.
AU  - Tims, Sebastian
AU  - Zoetendal, Erwin G.
AU  - de Vos, Willem M.
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2518
AB  - The microbiota that colonizes the human intestinal tract is complex and its structure is specific for each of us. In this study we expand the knowledge about the stability of the subject-specific microbiota and show that this ecosystem is stable in short-term intervals ( lt 1 year) but also during long periods of time ( gt 10 years). The faecal microbiota composition of five unrelated and healthy subjects was analysed using a comprehensive and highly reproducible phylogenetic microarray, the HITChip. The results show that the use of antibiotics, application of specific dietary regimes and distant travelling have limited impact on the microbiota composition. Several anaerobic genera, including Bifidobacterium and a number of genera within the Bacteroidetes and the Firmicutes phylum, exhibit significantly higher similarity than the total microbiota. Although the gut microbiota contains subject-specific species, the presence of which is preserved throughout the years, their relative abundance changes considerably. Consequently, the recently proposed enterotype status appears to be a varying characteristic of the microbiota. Our data show that the intestinal microbiota contains a core community of permanent colonizers, and that environmentally introduced changes of the microbiota throughout adulthood are primarily affecting the abundance but not the presence of specific microbial species.
PB  - Wiley, Hoboken
T2  - Environmental Microbiology
T1  - Long-term monitoring of the human intestinal microbiota composition
EP  - 1159
IS  - 4
SP  - 1146
VL  - 15
DO  - 10.1111/1462-2920.12023
ER  - 

@article{
author = "Rajilić-Stojanović, Mirjana and Heilig, Hans G. H. J. and Tims, Sebastian and Zoetendal, Erwin G. and de Vos, Willem M.",
year = "2013",
abstract = "The microbiota that colonizes the human intestinal tract is complex and its structure is specific for each of us. In this study we expand the knowledge about the stability of the subject-specific microbiota and show that this ecosystem is stable in short-term intervals ( lt 1 year) but also during long periods of time ( gt 10 years). The faecal microbiota composition of five unrelated and healthy subjects was analysed using a comprehensive and highly reproducible phylogenetic microarray, the HITChip. The results show that the use of antibiotics, application of specific dietary regimes and distant travelling have limited impact on the microbiota composition. Several anaerobic genera, including Bifidobacterium and a number of genera within the Bacteroidetes and the Firmicutes phylum, exhibit significantly higher similarity than the total microbiota. Although the gut microbiota contains subject-specific species, the presence of which is preserved throughout the years, their relative abundance changes considerably. Consequently, the recently proposed enterotype status appears to be a varying characteristic of the microbiota. Our data show that the intestinal microbiota contains a core community of permanent colonizers, and that environmentally introduced changes of the microbiota throughout adulthood are primarily affecting the abundance but not the presence of specific microbial species.",
publisher = "Wiley, Hoboken",
journal = "Environmental Microbiology",
title = "Long-term monitoring of the human intestinal microbiota composition",
pages = "1159-1146",
number = "4",
volume = "15",
doi = "10.1111/1462-2920.12023"
}

Rajilić-Stojanović, M., Heilig, H. G. H. J., Tims, S., Zoetendal, E. G.,& de Vos, W. M.. (2013). Long-term monitoring of the human intestinal microbiota composition. in Environmental Microbiology
Wiley, Hoboken., 15(4), 1146-1159.
https://doi.org/10.1111/1462-2920.12023

Rajilić-Stojanović M, Heilig HGHJ, Tims S, Zoetendal EG, de Vos WM. Long-term monitoring of the human intestinal microbiota composition. in Environmental Microbiology. 2013;15(4):1146-1159.
doi:10.1111/1462-2920.12023 .

Rajilić-Stojanović, Mirjana, Heilig, Hans G. H. J., Tims, Sebastian, Zoetendal, Erwin G., de Vos, Willem M., "Long-term monitoring of the human intestinal microbiota composition" in Environmental Microbiology, 15, no. 4 (2013):1146-1159,
https://doi.org/10.1111/1462-2920.12023 . .

TY  - JOUR
AU  - Booijink, Carien C. G. M.
AU  - El-Aidy, Sahar
AU  - Rajilić-Stojanović, Mirjana
AU  - Heilig, Hans G. H. J.
AU  - Troost, Freddy J.
AU  - Smidt, Hauke
AU  - Kleerebezem, Michiel
AU  - de Vos, Willem M.
AU  - Zoetendal, Erwin G.
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1735
AB  - P gt The diversity and temporal stability of the predominant bacteria in the human ileum was studied with the use of ileal effluent samples of seven individuals with Brooke ileostomies. The total number of bacteria within the ileal effluent was in the range of 107-108 bacteria per gram (wet weight). The diversity of the bacteria in the ileal effluent showed marked differences compared with that in faecal samples from age-matched healthy adults. The ileal effluent had a higher relative abundance of species within the orders Lactobacillales and Clostridiales, mainly Streptococcus bovis-related species, and the Veillonella group, and a lower proportion of species related to Ruminococcus gnavus, R. obeum and Bacteroides plebeius. In addition, inter-individual differences were found, indicative of a highly personal ileal microbiota profile. Furthermore, temporal profiles showed large fluctuations per individual over a period of 9-28 days (average similarity over a period of 9 days was as low as 44%), and differences between morning and afternoon profiles were observed. Parallel cloning and sequencing efforts revealed several phylotypes that were not identified in previous studies (12 out of 65 phylotypes showed less than 97% sequence similarity with previously reported sequences). Achaea were found to be below detection limit by quantitative PCR. Overall, the results indicate that the microbiota of the human ileum is relatively unstable, less complex and consisting of different dominating phylotypes when compared with the colonic microbiota.
PB  - Wiley, Hoboken
T2  - Environmental Microbiology
T1  - High temporal and inter-individual variation detected in the human ileal microbiota
EP  - 3227
IS  - 12
SP  - 3213
VL  - 12
DO  - 10.1111/j.1462-2920.2010.02294.x
ER  - 

@article{
author = "Booijink, Carien C. G. M. and El-Aidy, Sahar and Rajilić-Stojanović, Mirjana and Heilig, Hans G. H. J. and Troost, Freddy J. and Smidt, Hauke and Kleerebezem, Michiel and de Vos, Willem M. and Zoetendal, Erwin G.",
year = "2010",
abstract = "P gt The diversity and temporal stability of the predominant bacteria in the human ileum was studied with the use of ileal effluent samples of seven individuals with Brooke ileostomies. The total number of bacteria within the ileal effluent was in the range of 107-108 bacteria per gram (wet weight). The diversity of the bacteria in the ileal effluent showed marked differences compared with that in faecal samples from age-matched healthy adults. The ileal effluent had a higher relative abundance of species within the orders Lactobacillales and Clostridiales, mainly Streptococcus bovis-related species, and the Veillonella group, and a lower proportion of species related to Ruminococcus gnavus, R. obeum and Bacteroides plebeius. In addition, inter-individual differences were found, indicative of a highly personal ileal microbiota profile. Furthermore, temporal profiles showed large fluctuations per individual over a period of 9-28 days (average similarity over a period of 9 days was as low as 44%), and differences between morning and afternoon profiles were observed. Parallel cloning and sequencing efforts revealed several phylotypes that were not identified in previous studies (12 out of 65 phylotypes showed less than 97% sequence similarity with previously reported sequences). Achaea were found to be below detection limit by quantitative PCR. Overall, the results indicate that the microbiota of the human ileum is relatively unstable, less complex and consisting of different dominating phylotypes when compared with the colonic microbiota.",
publisher = "Wiley, Hoboken",
journal = "Environmental Microbiology",
title = "High temporal and inter-individual variation detected in the human ileal microbiota",
pages = "3227-3213",
number = "12",
volume = "12",
doi = "10.1111/j.1462-2920.2010.02294.x"
}

Booijink, C. C. G. M., El-Aidy, S., Rajilić-Stojanović, M., Heilig, H. G. H. J., Troost, F. J., Smidt, H., Kleerebezem, M., de Vos, W. M.,& Zoetendal, E. G.. (2010). High temporal and inter-individual variation detected in the human ileal microbiota. in Environmental Microbiology
Wiley, Hoboken., 12(12), 3213-3227.
https://doi.org/10.1111/j.1462-2920.2010.02294.x

Booijink CCGM, El-Aidy S, Rajilić-Stojanović M, Heilig HGHJ, Troost FJ, Smidt H, Kleerebezem M, de Vos WM, Zoetendal EG. High temporal and inter-individual variation detected in the human ileal microbiota. in Environmental Microbiology. 2010;12(12):3213-3227.
doi:10.1111/j.1462-2920.2010.02294.x .

Booijink, Carien C. G. M., El-Aidy, Sahar, Rajilić-Stojanović, Mirjana, Heilig, Hans G. H. J., Troost, Freddy J., Smidt, Hauke, Kleerebezem, Michiel, de Vos, Willem M., Zoetendal, Erwin G., "High temporal and inter-individual variation detected in the human ileal microbiota" in Environmental Microbiology, 12, no. 12 (2010):3213-3227,
https://doi.org/10.1111/j.1462-2920.2010.02294.x . .

TY  - CHAP
AU  - Rajilić-Stojanović, Mirjana
AU  - de Vos, Willem M.
AU  - Zoetendal, Erwin
PY  - 2008
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6384
PB  - ASM Press
T2  - Accessing uncultivated microorganisms: from the environment to organisms and genomes and back
T1  - The Human Intestinal Microbiota and Its Impact on Human Health
EP  - 32
SP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6384
ER  - 

@inbook{
author = "Rajilić-Stojanović, Mirjana and de Vos, Willem M. and Zoetendal, Erwin",
year = "2008",
publisher = "ASM Press",
journal = "Accessing uncultivated microorganisms: from the environment to organisms and genomes and back",
booktitle = "The Human Intestinal Microbiota and Its Impact on Human Health",
pages = "32-11",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6384"
}

Rajilić-Stojanović, M., de Vos, W. M.,& Zoetendal, E.. (2008). The Human Intestinal Microbiota and Its Impact on Human Health. in Accessing uncultivated microorganisms: from the environment to organisms and genomes and back
ASM Press., 11-32.
https://hdl.handle.net/21.15107/rcub_technorep_6384

Rajilić-Stojanović M, de Vos WM, Zoetendal E. The Human Intestinal Microbiota and Its Impact on Human Health. in Accessing uncultivated microorganisms: from the environment to organisms and genomes and back. 2008;:11-32.
https://hdl.handle.net/21.15107/rcub_technorep_6384 .

Rajilić-Stojanović, Mirjana, de Vos, Willem M., Zoetendal, Erwin, "The Human Intestinal Microbiota and Its Impact on Human Health" in Accessing uncultivated microorganisms: from the environment to organisms and genomes and back (2008):11-32,
https://hdl.handle.net/21.15107/rcub_technorep_6384 .

TY  - JOUR
AU  - Kajander, Kajsa
AU  - Myllyluoma, E.
AU  - Rajilić-Stojanović, Mirjana
AU  - Kyronpalo, S.
AU  - Rasmussen, M.
AU  - Jarvenpaa, S.
AU  - Zoetendal, Erwin G.
AU  - de Vos, Willem M.
AU  - Vapaatalo, H.
AU  - Korpela, R.
PY  - 2008
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1269
AB  - Background Irritable bowel syndrome is the most common diagnosis in gastroenterology. Trials suggest certain probiotics to be beneficial. Aim To investigate the effects of multispecies probiotic supplementation (Lactobacillus rhamnosus GG, L. rhamnosus Lc705, Propionibacterium freudenreichii ssp. shermanii JS and Bifidobacterium animalis ssp. lactis Bb 12) on abdominal symptoms, quality of life, intestinal microbiota and inflammatory markers in irritable bowel syndrome. Methods Eighty-six irritable bowel syndrome patients (Rome 11 criteria) participated in this randomized, placebo-controlled 5-month intervention. Patients were randomized to receive daily either multispecies probiotic supplementation or placebo. Irritable bowel syndrome symptoms, quality of life, microarray-based intestinal microbiota stability (n = 20), serum cytokines and sensitive C-reactive protein were monitored. Results The composite irritable bowel syndrome score had at 5 months decreased 14 points (95% CI: -19 to -9) from baseline with the multispecies probiotic vs. three points (95% CI: -8 to 1) with placebo (P = 0.0083). Especially, distension and abdominal pain were affected. A stabilization of the microbiota was observed, as the microbiota similarity index increased with the probiotic supplementation (1.9 +/- 3.1), while it decreased with placebo (-2.9 +/- 1.7). No differences were seen in C-reactive protein. Conclusions This multispecies probiotic seems to be an effective and safe option to alleviate symptoms of irritable bowel syndrome, and to stabilize the intestinal microbiota.
PB  - Wiley, Hoboken
T2  - Alimentary Pharmacology & Therapeutics
T1  - Clinical trial: multispecies probiotic supplementation alleviates the symptoms of irritable bowel syndrome and stabilizes intestinal microbiota
EP  - 57
IS  - 1
SP  - 48
VL  - 27
DO  - 10.1111/j.1365-2036.2007.03542.x
ER  - 

@article{
author = "Kajander, Kajsa and Myllyluoma, E. and Rajilić-Stojanović, Mirjana and Kyronpalo, S. and Rasmussen, M. and Jarvenpaa, S. and Zoetendal, Erwin G. and de Vos, Willem M. and Vapaatalo, H. and Korpela, R.",
year = "2008",
abstract = "Background Irritable bowel syndrome is the most common diagnosis in gastroenterology. Trials suggest certain probiotics to be beneficial. Aim To investigate the effects of multispecies probiotic supplementation (Lactobacillus rhamnosus GG, L. rhamnosus Lc705, Propionibacterium freudenreichii ssp. shermanii JS and Bifidobacterium animalis ssp. lactis Bb 12) on abdominal symptoms, quality of life, intestinal microbiota and inflammatory markers in irritable bowel syndrome. Methods Eighty-six irritable bowel syndrome patients (Rome 11 criteria) participated in this randomized, placebo-controlled 5-month intervention. Patients were randomized to receive daily either multispecies probiotic supplementation or placebo. Irritable bowel syndrome symptoms, quality of life, microarray-based intestinal microbiota stability (n = 20), serum cytokines and sensitive C-reactive protein were monitored. Results The composite irritable bowel syndrome score had at 5 months decreased 14 points (95% CI: -19 to -9) from baseline with the multispecies probiotic vs. three points (95% CI: -8 to 1) with placebo (P = 0.0083). Especially, distension and abdominal pain were affected. A stabilization of the microbiota was observed, as the microbiota similarity index increased with the probiotic supplementation (1.9 +/- 3.1), while it decreased with placebo (-2.9 +/- 1.7). No differences were seen in C-reactive protein. Conclusions This multispecies probiotic seems to be an effective and safe option to alleviate symptoms of irritable bowel syndrome, and to stabilize the intestinal microbiota.",
publisher = "Wiley, Hoboken",
journal = "Alimentary Pharmacology & Therapeutics",
title = "Clinical trial: multispecies probiotic supplementation alleviates the symptoms of irritable bowel syndrome and stabilizes intestinal microbiota",
pages = "57-48",
number = "1",
volume = "27",
doi = "10.1111/j.1365-2036.2007.03542.x"
}

Kajander, K., Myllyluoma, E., Rajilić-Stojanović, M., Kyronpalo, S., Rasmussen, M., Jarvenpaa, S., Zoetendal, E. G., de Vos, W. M., Vapaatalo, H.,& Korpela, R.. (2008). Clinical trial: multispecies probiotic supplementation alleviates the symptoms of irritable bowel syndrome and stabilizes intestinal microbiota. in Alimentary Pharmacology & Therapeutics
Wiley, Hoboken., 27(1), 48-57.
https://doi.org/10.1111/j.1365-2036.2007.03542.x

Kajander K, Myllyluoma E, Rajilić-Stojanović M, Kyronpalo S, Rasmussen M, Jarvenpaa S, Zoetendal EG, de Vos WM, Vapaatalo H, Korpela R. Clinical trial: multispecies probiotic supplementation alleviates the symptoms of irritable bowel syndrome and stabilizes intestinal microbiota. in Alimentary Pharmacology & Therapeutics. 2008;27(1):48-57.
doi:10.1111/j.1365-2036.2007.03542.x .

Kajander, Kajsa, Myllyluoma, E., Rajilić-Stojanović, Mirjana, Kyronpalo, S., Rasmussen, M., Jarvenpaa, S., Zoetendal, Erwin G., de Vos, Willem M., Vapaatalo, H., Korpela, R., "Clinical trial: multispecies probiotic supplementation alleviates the symptoms of irritable bowel syndrome and stabilizes intestinal microbiota" in Alimentary Pharmacology & Therapeutics, 27, no. 1 (2008):48-57,
https://doi.org/10.1111/j.1365-2036.2007.03542.x . .

TY  - JOUR
AU  - Zoetendal, Erwin G.
AU  - Rajilić-Stojanović, Mirjana
AU  - de Vos, Willem M.
PY  - 2008
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1226
AB  - The human gastrointestinal (GI) tract microbiota plays a pivotal role in our health. For more than a decade a major input for describing the diversity of the GI tract microbiota has been derived from the application of small subunit ribosomal RNA (SSU rRNA)-based technologies. These not only provided a phylogenetic framework of the GI tract microbiota, the majority of which has not yet been cultured, but also advanced insights into the impact of host and environmental factors on the microbiota community structure and dynamics. In addition, it emerged that GI tract microbial communities are host and GI tract location-specific. This complicates establishing relevant links between the host's health and the presence or abundance of specific microbial populations and argues for the implementation of novel high-throughput technologies in studying the diversity and functionality of the GI tract microbiota. Here, we focus on the recent developments and applications of phylogenetic microarrays based on SSU rRNA sequences and metagenomics approaches exploiting rapid sequencing technologies in unravelling the secrets of our GI tract microbiota.
PB  - Bmj Publishing Group, London
T2  - GUT
T1  - High-throughput diversity and functionality analysis of the gastrointestinal tract microbiota
EP  - 1615
IS  - 11
SP  - 1605
VL  - 57
DO  - 10.1136/gut.2007.133603
ER  - 

@article{
author = "Zoetendal, Erwin G. and Rajilić-Stojanović, Mirjana and de Vos, Willem M.",
year = "2008",
abstract = "The human gastrointestinal (GI) tract microbiota plays a pivotal role in our health. For more than a decade a major input for describing the diversity of the GI tract microbiota has been derived from the application of small subunit ribosomal RNA (SSU rRNA)-based technologies. These not only provided a phylogenetic framework of the GI tract microbiota, the majority of which has not yet been cultured, but also advanced insights into the impact of host and environmental factors on the microbiota community structure and dynamics. In addition, it emerged that GI tract microbial communities are host and GI tract location-specific. This complicates establishing relevant links between the host's health and the presence or abundance of specific microbial populations and argues for the implementation of novel high-throughput technologies in studying the diversity and functionality of the GI tract microbiota. Here, we focus on the recent developments and applications of phylogenetic microarrays based on SSU rRNA sequences and metagenomics approaches exploiting rapid sequencing technologies in unravelling the secrets of our GI tract microbiota.",
publisher = "Bmj Publishing Group, London",
journal = "GUT",
title = "High-throughput diversity and functionality analysis of the gastrointestinal tract microbiota",
pages = "1615-1605",
number = "11",
volume = "57",
doi = "10.1136/gut.2007.133603"
}

Zoetendal, E. G., Rajilić-Stojanović, M.,& de Vos, W. M.. (2008). High-throughput diversity and functionality analysis of the gastrointestinal tract microbiota. in GUT
Bmj Publishing Group, London., 57(11), 1605-1615.
https://doi.org/10.1136/gut.2007.133603

Zoetendal EG, Rajilić-Stojanović M, de Vos WM. High-throughput diversity and functionality analysis of the gastrointestinal tract microbiota. in GUT. 2008;57(11):1605-1615.
doi:10.1136/gut.2007.133603 .

Zoetendal, Erwin G., Rajilić-Stojanović, Mirjana, de Vos, Willem M., "High-throughput diversity and functionality analysis of the gastrointestinal tract microbiota" in GUT, 57, no. 11 (2008):1605-1615,
https://doi.org/10.1136/gut.2007.133603 . .