TY  - CONF
AU  - Marković, Maja D.
AU  - Svetozarević, Milica M.
AU  - Meng, Huan
AU  - Li, Zhen
AU  - Grad, Sibylle
AU  - Panić, Vesna V.
AU  - Kalagasidis Krušić, Melina T.
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7391
AB  - INTRODUCTION: Inflammation  process  in  human  body  can  lead  to  many serious inflammation-related  diseases. Hence, there are urge to find better solution for the treatment of the inflammation processes. Solution can be found in application  ofpoly(methacrylicacid)  hydrogels  which  have  potential  for  targeted  delivery  and  controlled  release  of drugs. These pH-sensitive hydrogelscan swell at the pH values between 5 and 8,and release drug in the process. So,taking into account that pH value at the inflammation site is around 6, these hydrogels are materials of choice. It  is  very  important thatthe system  for  controlled  releasebe  prepared  through mild  and  non-toxic  conditions  in order to preserve bioactivity of the drug andkeep good impact on environment. Enzymes are good candidates for eco-friendly preparation of hydrogels, because these greensubstancescan initiate polymerisation of various monomers. 
EXPERIMENTAL (or Materials and Methods):In this study, hydrogels based on poly(methacrylic acid) were prepared through  eco-friendly  method  by  using enzyme/hydrogen  peroxide(HP)/ascorbic  acid(AA)as  initiator.  Two  groupsof the samples were prepared: in the first group peroxidase isolated from potato peel waste (with activity of 0.8 IU) was used in the initiation system, whereas in the second group peroxidase isolated from soya bean coats (with activity of 0.8  IU) was  employed  in  the  initiation  system.  The  amounts  of HPand AAin  both  series  were  40  μL  and  10  mg, respectively.Anti-inflammatory drugs,dexamethasone(5 mg/mL)and diclofenac(4.5 mg/mL)were encapsulated in the first and the second group of the PMAA samples, respectively. Anti-inflammatory  effect of the PMAA hydrogels  with encapsulated drugs were tested on the Bovine Chondrocytes cells.
RESULTS  AND  DISCUSSION:Results  showed  that  the  level  of  pro-inflammatory  mediators  NO  and  IL-8  decreased. The PCR analysis showed that the proinflammatory TNα, IL-6 genesexpression level decreased, the matrix catabolism MMP1and MMP3 genesexpression level decreased and the fibrotic COL2 and ACAN genes expression level increased.
CONCLUSIONS:The studyshowed that the PMAA hydrogels have anti-inflammatory effect and have potential for treatment of the inflammation processes.
PB  - Savez hemijskih inženjera Srbije
C3  - Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts
T1  - Biocharacterization of hydrogels based on poly(methacrylic acid) prepared by eco-friendly method
IS  - 1S
SP  - 28
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7391
ER  - 

@conference{
author = "Marković, Maja D. and Svetozarević, Milica M. and Meng, Huan and Li, Zhen and Grad, Sibylle and Panić, Vesna V. and Kalagasidis Krušić, Melina T.",
year = "2024",
abstract = "INTRODUCTION: Inflammation  process  in  human  body  can  lead  to  many serious inflammation-related  diseases. Hence, there are urge to find better solution for the treatment of the inflammation processes. Solution can be found in application  ofpoly(methacrylicacid)  hydrogels  which  have  potential  for  targeted  delivery  and  controlled  release  of drugs. These pH-sensitive hydrogelscan swell at the pH values between 5 and 8,and release drug in the process. So,taking into account that pH value at the inflammation site is around 6, these hydrogels are materials of choice. It  is  very  important thatthe system  for  controlled  releasebe  prepared  through mild  and  non-toxic  conditions  in order to preserve bioactivity of the drug andkeep good impact on environment. Enzymes are good candidates for eco-friendly preparation of hydrogels, because these greensubstancescan initiate polymerisation of various monomers. 
EXPERIMENTAL (or Materials and Methods):In this study, hydrogels based on poly(methacrylic acid) were prepared through  eco-friendly  method  by  using enzyme/hydrogen  peroxide(HP)/ascorbic  acid(AA)as  initiator.  Two  groupsof the samples were prepared: in the first group peroxidase isolated from potato peel waste (with activity of 0.8 IU) was used in the initiation system, whereas in the second group peroxidase isolated from soya bean coats (with activity of 0.8  IU) was  employed  in  the  initiation  system.  The  amounts  of HPand AAin  both  series  were  40  μL  and  10  mg, respectively.Anti-inflammatory drugs,dexamethasone(5 mg/mL)and diclofenac(4.5 mg/mL)were encapsulated in the first and the second group of the PMAA samples, respectively. Anti-inflammatory  effect of the PMAA hydrogels  with encapsulated drugs were tested on the Bovine Chondrocytes cells.
RESULTS  AND  DISCUSSION:Results  showed  that  the  level  of  pro-inflammatory  mediators  NO  and  IL-8  decreased. The PCR analysis showed that the proinflammatory TNα, IL-6 genesexpression level decreased, the matrix catabolism MMP1and MMP3 genesexpression level decreased and the fibrotic COL2 and ACAN genes expression level increased.
CONCLUSIONS:The studyshowed that the PMAA hydrogels have anti-inflammatory effect and have potential for treatment of the inflammation processes.",
publisher = "Savez hemijskih inženjera Srbije",
journal = "Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts",
title = "Biocharacterization of hydrogels based on poly(methacrylic acid) prepared by eco-friendly method",
number = "1S",
pages = "28",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7391"
}

Marković, M. D., Svetozarević, M. M., Meng, H., Li, Z., Grad, S., Panić, V. V.,& Kalagasidis Krušić, M. T.. (2024). Biocharacterization of hydrogels based on poly(methacrylic acid) prepared by eco-friendly method. in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts
Savez hemijskih inženjera Srbije., 78(1S), 28.
https://hdl.handle.net/21.15107/rcub_technorep_7391

Marković MD, Svetozarević MM, Meng H, Li Z, Grad S, Panić VV, Kalagasidis Krušić MT. Biocharacterization of hydrogels based on poly(methacrylic acid) prepared by eco-friendly method. in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts. 2024;78(1S):28.
https://hdl.handle.net/21.15107/rcub_technorep_7391 .

Marković, Maja D., Svetozarević, Milica M., Meng, Huan, Li, Zhen, Grad, Sibylle, Panić, Vesna V., Kalagasidis Krušić, Melina T., "Biocharacterization of hydrogels based on poly(methacrylic acid) prepared by eco-friendly method" in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts, 78, no. 1S (2024):28,
https://hdl.handle.net/21.15107/rcub_technorep_7391 .

TY  - CONF
AU  - Marković, Maja D.
AU  - Svetozarević, Milica M.
AU  - Ugrinović, Vukašin Đ.
AU  - Pjanović, Rada V.
AU  - Obradović, Bojana M.
AU  - Kalagasidis Krušić, Melina T.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6620
AB  - Everyday struggle of humanity with novel diseases and present once, urge researchers to find novel and improve existing therapies to enhance their efficiency and safety. One of the promising approaches to overcome these challenges is controlled release of drugs. Biomaterials based on poly(methacrylic acid) (PMAA) are excellent drug delivery systems because they can control release rate and released amount of drug. Also, due to their pH sensitivity the PMAA hydrogels can release drug at the site of action. Namely, these nontoxic and biocompatible hydrogels swell in the environment with pH value higher than pKa of PMAA (4.6) and release encapsulated drug during the process. In present study, PMAA hydrogels are synthetized under ambient conditions by simple, cost effective and eco-friendly synthesis. Novel initiation system based on hydrogen peroxide, potato peel peroxidase and vitamin C (VC) is used for the first time for free radical polymerization of PMAA hydrogel. In accordance with the principles of circular economy, peroxidase was isolated from potato peel waste by water extraction for 12 h at 4 ℃. Four PMAA hydrogels were prepared by using potato peel peroxidase with various enzyme activity (0.4; 0.8; 1.2 and 1.8 IU), whereas the amounts of H2O2 (30 mL) and VC (10 mg) have been kept constant. The composition of the PMAA hydrogels was confirmed by FTIR analysis, whereas their porous structure was revealed by SEM. The swelling of the PMAA hydrogels was monitored in two media: 0.1M HCl (as simulation of human stomach) and phosphate buffer with pH 7.4 (as simulation of human intestines). In order to encapsulate anti-inflammatory drug – dexamethasone into the PMAA hydrogels, the hydrogels were immersed into the dexamethasone aqueous solution (5 mg/ml) and left to swell to the equilibrium, after which they were dried at room temperature. Dexamethasone release from the PMAA hydrogels was monitored in the same environments as was the PMAA swelling. The results showed that around seven times higher amount of dexamethasone was released in the medium with pH 7.4 than in the medium with pH 1. Present study has showed that the PMAA hydrogels, prepared through green and eco-friendly method, have huge potential for encapsulation and controlled release of anti-inflammatory drugs and therefore, for the treatment of rheumatoid arthritis.
PB  - AO Foundation
C3  - ESB2023: 33rd Annual Conference of the European Society for Biomaterials, ARI Abstracts Periodical
T1  - Hydrogels based on poly(methacrylic acid) for controlled release of anti-inflammatory drugs
SP  - 572
VL  - 3
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6620
ER  - 

@conference{
author = "Marković, Maja D. and Svetozarević, Milica M. and Ugrinović, Vukašin Đ. and Pjanović, Rada V. and Obradović, Bojana M. and Kalagasidis Krušić, Melina T.",
year = "2023",
abstract = "Everyday struggle of humanity with novel diseases and present once, urge researchers to find novel and improve existing therapies to enhance their efficiency and safety. One of the promising approaches to overcome these challenges is controlled release of drugs. Biomaterials based on poly(methacrylic acid) (PMAA) are excellent drug delivery systems because they can control release rate and released amount of drug. Also, due to their pH sensitivity the PMAA hydrogels can release drug at the site of action. Namely, these nontoxic and biocompatible hydrogels swell in the environment with pH value higher than pKa of PMAA (4.6) and release encapsulated drug during the process. In present study, PMAA hydrogels are synthetized under ambient conditions by simple, cost effective and eco-friendly synthesis. Novel initiation system based on hydrogen peroxide, potato peel peroxidase and vitamin C (VC) is used for the first time for free radical polymerization of PMAA hydrogel. In accordance with the principles of circular economy, peroxidase was isolated from potato peel waste by water extraction for 12 h at 4 ℃. Four PMAA hydrogels were prepared by using potato peel peroxidase with various enzyme activity (0.4; 0.8; 1.2 and 1.8 IU), whereas the amounts of H2O2 (30 mL) and VC (10 mg) have been kept constant. The composition of the PMAA hydrogels was confirmed by FTIR analysis, whereas their porous structure was revealed by SEM. The swelling of the PMAA hydrogels was monitored in two media: 0.1M HCl (as simulation of human stomach) and phosphate buffer with pH 7.4 (as simulation of human intestines). In order to encapsulate anti-inflammatory drug – dexamethasone into the PMAA hydrogels, the hydrogels were immersed into the dexamethasone aqueous solution (5 mg/ml) and left to swell to the equilibrium, after which they were dried at room temperature. Dexamethasone release from the PMAA hydrogels was monitored in the same environments as was the PMAA swelling. The results showed that around seven times higher amount of dexamethasone was released in the medium with pH 7.4 than in the medium with pH 1. Present study has showed that the PMAA hydrogels, prepared through green and eco-friendly method, have huge potential for encapsulation and controlled release of anti-inflammatory drugs and therefore, for the treatment of rheumatoid arthritis.",
publisher = "AO Foundation",
journal = "ESB2023: 33rd Annual Conference of the European Society for Biomaterials, ARI Abstracts Periodical",
title = "Hydrogels based on poly(methacrylic acid) for controlled release of anti-inflammatory drugs",
pages = "572",
volume = "3",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6620"
}

Marković, M. D., Svetozarević, M. M., Ugrinović, V. Đ., Pjanović, R. V., Obradović, B. M.,& Kalagasidis Krušić, M. T.. (2023). Hydrogels based on poly(methacrylic acid) for controlled release of anti-inflammatory drugs. in ESB2023: 33rd Annual Conference of the European Society for Biomaterials, ARI Abstracts Periodical
AO Foundation., 3, 572.
https://hdl.handle.net/21.15107/rcub_technorep_6620

Marković MD, Svetozarević MM, Ugrinović VĐ, Pjanović RV, Obradović BM, Kalagasidis Krušić MT. Hydrogels based on poly(methacrylic acid) for controlled release of anti-inflammatory drugs. in ESB2023: 33rd Annual Conference of the European Society for Biomaterials, ARI Abstracts Periodical. 2023;3:572.
https://hdl.handle.net/21.15107/rcub_technorep_6620 .

Marković, Maja D., Svetozarević, Milica M., Ugrinović, Vukašin Đ., Pjanović, Rada V., Obradović, Bojana M., Kalagasidis Krušić, Melina T., "Hydrogels based on poly(methacrylic acid) for controlled release of anti-inflammatory drugs" in ESB2023: 33rd Annual Conference of the European Society for Biomaterials, ARI Abstracts Periodical, 3 (2023):572,
https://hdl.handle.net/21.15107/rcub_technorep_6620 .