TY  - JOUR
AU  - Žuža, Milena
AU  - Šiler-Marinković, Slavica
AU  - Knežević, Zorica
PY  - 2007
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1061
AB  - This paper reports the covalent immobilization of penicillin G acylase from E. coli on Sepabeads EC-EP, an epoxy-activated polymethacrylic carrier, and describes the properties of the immobilized enzyme. Due to its versatility to mediate hydrolysis of penicillins and semi-synthetic B-lactam antibiotics synthesis reactions, the selected enzyme belongs to a class of biocatalysts of great industrial interest. The immobilized enzyme was characterized in its pH and thermal stability and reaction kinetics. The immobilization of penicillin acylase resulted in a slightly different pH activity profile and temperature optima, indicating that the immobilization by this method imparted the structural and conformational stability to this enzyme. The immobilized enzyme also retained a high catalytic activity and showed the increased thermal stability compared with a free enzyme. By comparison of decimal reduction time values obtained at 50°C, it can be concluded that the immobilized enzyme was approximately 5-fold more stable than a free enzyme. The immobilization procedure developed is quite simple and easily reproduced, and provides a promising solution for the application of penicillin acylase for the purpose of 6-aminopenicillanic acid production.
PB  - Association of the Chemical Engineers of Serbia
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Preparation and characterization of penicillin acylase immobilized on sepabeads EC-EP carrier
EP  - 210
IS  - 4
SP  - 205
VL  - 13
DO  - 10.2298/CICEQ0704205Z
ER  - 

@article{
author = "Žuža, Milena and Šiler-Marinković, Slavica and Knežević, Zorica",
year = "2007",
abstract = "This paper reports the covalent immobilization of penicillin G acylase from E. coli on Sepabeads EC-EP, an epoxy-activated polymethacrylic carrier, and describes the properties of the immobilized enzyme. Due to its versatility to mediate hydrolysis of penicillins and semi-synthetic B-lactam antibiotics synthesis reactions, the selected enzyme belongs to a class of biocatalysts of great industrial interest. The immobilized enzyme was characterized in its pH and thermal stability and reaction kinetics. The immobilization of penicillin acylase resulted in a slightly different pH activity profile and temperature optima, indicating that the immobilization by this method imparted the structural and conformational stability to this enzyme. The immobilized enzyme also retained a high catalytic activity and showed the increased thermal stability compared with a free enzyme. By comparison of decimal reduction time values obtained at 50°C, it can be concluded that the immobilized enzyme was approximately 5-fold more stable than a free enzyme. The immobilization procedure developed is quite simple and easily reproduced, and provides a promising solution for the application of penicillin acylase for the purpose of 6-aminopenicillanic acid production.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Preparation and characterization of penicillin acylase immobilized on sepabeads EC-EP carrier",
pages = "210-205",
number = "4",
volume = "13",
doi = "10.2298/CICEQ0704205Z"
}

Žuža, M., Šiler-Marinković, S.,& Knežević, Z.. (2007). Preparation and characterization of penicillin acylase immobilized on sepabeads EC-EP carrier. in Chemical Industry & Chemical Engineering Quarterly
Association of the Chemical Engineers of Serbia., 13(4), 205-210.
https://doi.org/10.2298/CICEQ0704205Z

Žuža M, Šiler-Marinković S, Knežević Z. Preparation and characterization of penicillin acylase immobilized on sepabeads EC-EP carrier. in Chemical Industry & Chemical Engineering Quarterly. 2007;13(4):205-210.
doi:10.2298/CICEQ0704205Z .

Žuža, Milena, Šiler-Marinković, Slavica, Knežević, Zorica, "Preparation and characterization of penicillin acylase immobilized on sepabeads EC-EP carrier" in Chemical Industry & Chemical Engineering Quarterly, 13, no. 4 (2007):205-210,
https://doi.org/10.2298/CICEQ0704205Z . .