TY  - JOUR
AU  - Smiljanić, Katarina
AU  - Prodić, Ivana
AU  - Apostolović, Danijela
AU  - Cvetković, Anka
AU  - Veljović, Đorđe
AU  - Mutić, Jelena
AU  - van Hage, Marianne
AU  - Burazer, Lidija M.
AU  - Ćirković-Veličković, Tanja
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4169
AB  - An association between pollution (e.g., from traffic emissions) and the increased prevalence of respiratory allergies has been observed. Field-realistic exposure studies provide the most relevant assessment of the effects of the intensity and diversity of urban and industrial contamination on pollen structure and allergenicity. The significance of in-depth post-translational modification (PTM) studies of pollen proteomes, when compared with studies on other aspects of pollution and altered pollen allergenicity, has not yet been determined; hence, little progress has been made within this field. We undertook a comprehensive comparative analysis of multiple polluted and environmentally preserved Phleum pratense (Timothy grass) pollen samples using scanning electron microscopy, in-depth PTM profiling, determination of organic and inorganic pollutants, analysis of the release of sub-pollen particles and phenols/proteins, and analysis of proteome expression using high resolution tandem mass spectrometry. In addition, we used quantitative enzyme-linked immunosorbent assays (ELISA) and immunoglobulin E (IgE) immunoblotting. An increased phenolic content and release of sub-pollen particles was found in pollen samples from the polluted area, including a significantly higher content of mercury, cadmium, and manganese, with irregular long spines on pollen grain surface structures. Antioxidative defense-related enzymes were significantly upregulated and seven oxidative PTMs were significantly increased (methionine, histidine, lysine, and proline oxidation; tyrosine glycosylation, lysine 4-hydroxy-2-nonenal adduct, and lysine carbamylation) in pollen exposed to the chemical plant and road traffic pollution sources. Oxidative modifications affected several Timothy pollen allergens; Phl p 6, in particular, exhibited several different oxidative modifications. The expression of Phl p 6, 12, and 13 allergens were downregulated in polluted pollen, and IgE binding to pollen extract was substantially lower in the 18 patients studied, as measured by quantitative ELISA. Quantitative, unrestricted, and detailed PTM searches using an enrichment-free approach pointed to modification of Timothy pollen allergens and suggested that heavy metals are primarily responsible for oxidative stress effects observed in pollen proteins.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Environment International
T1  - In-depth quantitative profiling of post-translational modifications of Timothy grass pollen allergome in relation to environmental oxidative stress
EP  - 658
SP  - 644
VL  - 126
DO  - 10.1016/j.envint.2019.03.001
ER  - 

@article{
author = "Smiljanić, Katarina and Prodić, Ivana and Apostolović, Danijela and Cvetković, Anka and Veljović, Đorđe and Mutić, Jelena and van Hage, Marianne and Burazer, Lidija M. and Ćirković-Veličković, Tanja",
year = "2019",
abstract = "An association between pollution (e.g., from traffic emissions) and the increased prevalence of respiratory allergies has been observed. Field-realistic exposure studies provide the most relevant assessment of the effects of the intensity and diversity of urban and industrial contamination on pollen structure and allergenicity. The significance of in-depth post-translational modification (PTM) studies of pollen proteomes, when compared with studies on other aspects of pollution and altered pollen allergenicity, has not yet been determined; hence, little progress has been made within this field. We undertook a comprehensive comparative analysis of multiple polluted and environmentally preserved Phleum pratense (Timothy grass) pollen samples using scanning electron microscopy, in-depth PTM profiling, determination of organic and inorganic pollutants, analysis of the release of sub-pollen particles and phenols/proteins, and analysis of proteome expression using high resolution tandem mass spectrometry. In addition, we used quantitative enzyme-linked immunosorbent assays (ELISA) and immunoglobulin E (IgE) immunoblotting. An increased phenolic content and release of sub-pollen particles was found in pollen samples from the polluted area, including a significantly higher content of mercury, cadmium, and manganese, with irregular long spines on pollen grain surface structures. Antioxidative defense-related enzymes were significantly upregulated and seven oxidative PTMs were significantly increased (methionine, histidine, lysine, and proline oxidation; tyrosine glycosylation, lysine 4-hydroxy-2-nonenal adduct, and lysine carbamylation) in pollen exposed to the chemical plant and road traffic pollution sources. Oxidative modifications affected several Timothy pollen allergens; Phl p 6, in particular, exhibited several different oxidative modifications. The expression of Phl p 6, 12, and 13 allergens were downregulated in polluted pollen, and IgE binding to pollen extract was substantially lower in the 18 patients studied, as measured by quantitative ELISA. Quantitative, unrestricted, and detailed PTM searches using an enrichment-free approach pointed to modification of Timothy pollen allergens and suggested that heavy metals are primarily responsible for oxidative stress effects observed in pollen proteins.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Environment International",
title = "In-depth quantitative profiling of post-translational modifications of Timothy grass pollen allergome in relation to environmental oxidative stress",
pages = "658-644",
volume = "126",
doi = "10.1016/j.envint.2019.03.001"
}

Smiljanić, K., Prodić, I., Apostolović, D., Cvetković, A., Veljović, Đ., Mutić, J., van Hage, M., Burazer, L. M.,& Ćirković-Veličković, T.. (2019). In-depth quantitative profiling of post-translational modifications of Timothy grass pollen allergome in relation to environmental oxidative stress. in Environment International
Pergamon-Elsevier Science Ltd, Oxford., 126, 644-658.
https://doi.org/10.1016/j.envint.2019.03.001

Smiljanić K, Prodić I, Apostolović D, Cvetković A, Veljović Đ, Mutić J, van Hage M, Burazer LM, Ćirković-Veličković T. In-depth quantitative profiling of post-translational modifications of Timothy grass pollen allergome in relation to environmental oxidative stress. in Environment International. 2019;126:644-658.
doi:10.1016/j.envint.2019.03.001 .

Smiljanić, Katarina, Prodić, Ivana, Apostolović, Danijela, Cvetković, Anka, Veljović, Đorđe, Mutić, Jelena, van Hage, Marianne, Burazer, Lidija M., Ćirković-Veličković, Tanja, "In-depth quantitative profiling of post-translational modifications of Timothy grass pollen allergome in relation to environmental oxidative stress" in Environment International, 126 (2019):644-658,
https://doi.org/10.1016/j.envint.2019.03.001 . .

TY  - JOUR
AU  - Amanullah, R.
AU  - Johansson, J.
AU  - Goobar, A.
AU  - Ferretti, R.
AU  - Papadogiannakis, S.
AU  - Petrushevska, T.
AU  - Brown, P. J.
AU  - Cao, Y.
AU  - Contreras, C.
AU  - Dahle, H.
AU  - Elias-Rosa, N.
AU  - Fynbo, J. P. U.
AU  - Gorosabel, J.
AU  - Guaita, L.
AU  - Hangard, L.
AU  - Howell, D. A.
AU  - Hsiao, E. Y.
AU  - Kankare, E.
AU  - Kasliwal, M.
AU  - Leloudas, G.
AU  - Lundqvist, P.
AU  - Mattila, S.
AU  - Nugent, P.
AU  - Phillips, M. M.
AU  - Sandberg, A.
AU  - Stanishev, V.
AU  - Sullivan, M.
AU  - Taddia, F.
AU  - Ostlin, G.
AU  - Asadi, S.
AU  - Herrero-Illana, R.
AU  - Jensen, J. J.
AU  - Karhunen, K.
AU  - Lazarević, Slavica
AU  - Varenius, E.
AU  - Santos, P.
AU  - Sridhar, S. Seethapuram
AU  - Wallstrom, S. H. J.
AU  - Wiegert, J.
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3073
AB  - We present ultraviolet (UV) observations of six nearby Type Ia supernovae (SNe Ia) obtained with the Hubble Space Telescope, three of which were also observed in the near-IR (NIR) with Wide-Field Camera 3. UV observations with the Swift satellite, as well as ground-based optical and NIR data provide complementary information. The combined data set covers the wavelength range 0.2-2 mu m. By also including archival data of SN 2014J, we analyse a sample spanning observed colour excesses up to E(B - V) = 1.4 mag. We study the wavelength-dependent extinction of each individual SN and find a diversity of reddening laws when characterized by the total-to-selective extinction R-V. In particular, we note that for the two SNe with E(B - V) greater than or similar to 1 mag, for which the colour excess is dominated by dust extinction, we find R-V = 1.4 +/- 0.1 and R-V = 2.8 +/- 0.1. Adding UV photometry reduces the uncertainty of fitted R-V by similar to 50 per cent allowing us to also measure R-V of individual low-extinction objects which point to a similar diversity, currently not accounted for in the analyses when SNe Ia are used for studying the expansion history of the Universe.
PB  - Oxford Univ Press, Oxford
T2  - Monthly Notices of the Royal Astronomical Society
T1  - Diversity in extinction laws of Type Ia supernovae measured between 0.2 and 2 mu m
EP  - 3328
IS  - 3
SP  - 3300
VL  - 453
DO  - 10.1093/mnras/stv1505
ER  - 

@article{
author = "Amanullah, R. and Johansson, J. and Goobar, A. and Ferretti, R. and Papadogiannakis, S. and Petrushevska, T. and Brown, P. J. and Cao, Y. and Contreras, C. and Dahle, H. and Elias-Rosa, N. and Fynbo, J. P. U. and Gorosabel, J. and Guaita, L. and Hangard, L. and Howell, D. A. and Hsiao, E. Y. and Kankare, E. and Kasliwal, M. and Leloudas, G. and Lundqvist, P. and Mattila, S. and Nugent, P. and Phillips, M. M. and Sandberg, A. and Stanishev, V. and Sullivan, M. and Taddia, F. and Ostlin, G. and Asadi, S. and Herrero-Illana, R. and Jensen, J. J. and Karhunen, K. and Lazarević, Slavica and Varenius, E. and Santos, P. and Sridhar, S. Seethapuram and Wallstrom, S. H. J. and Wiegert, J.",
year = "2015",
abstract = "We present ultraviolet (UV) observations of six nearby Type Ia supernovae (SNe Ia) obtained with the Hubble Space Telescope, three of which were also observed in the near-IR (NIR) with Wide-Field Camera 3. UV observations with the Swift satellite, as well as ground-based optical and NIR data provide complementary information. The combined data set covers the wavelength range 0.2-2 mu m. By also including archival data of SN 2014J, we analyse a sample spanning observed colour excesses up to E(B - V) = 1.4 mag. We study the wavelength-dependent extinction of each individual SN and find a diversity of reddening laws when characterized by the total-to-selective extinction R-V. In particular, we note that for the two SNe with E(B - V) greater than or similar to 1 mag, for which the colour excess is dominated by dust extinction, we find R-V = 1.4 +/- 0.1 and R-V = 2.8 +/- 0.1. Adding UV photometry reduces the uncertainty of fitted R-V by similar to 50 per cent allowing us to also measure R-V of individual low-extinction objects which point to a similar diversity, currently not accounted for in the analyses when SNe Ia are used for studying the expansion history of the Universe.",
publisher = "Oxford Univ Press, Oxford",
journal = "Monthly Notices of the Royal Astronomical Society",
title = "Diversity in extinction laws of Type Ia supernovae measured between 0.2 and 2 mu m",
pages = "3328-3300",
number = "3",
volume = "453",
doi = "10.1093/mnras/stv1505"
}

Amanullah, R., Johansson, J., Goobar, A., Ferretti, R., Papadogiannakis, S., Petrushevska, T., Brown, P. J., Cao, Y., Contreras, C., Dahle, H., Elias-Rosa, N., Fynbo, J. P. U., Gorosabel, J., Guaita, L., Hangard, L., Howell, D. A., Hsiao, E. Y., Kankare, E., Kasliwal, M., Leloudas, G., Lundqvist, P., Mattila, S., Nugent, P., Phillips, M. M., Sandberg, A., Stanishev, V., Sullivan, M., Taddia, F., Ostlin, G., Asadi, S., Herrero-Illana, R., Jensen, J. J., Karhunen, K., Lazarević, S., Varenius, E., Santos, P., Sridhar, S. S., Wallstrom, S. H. J.,& Wiegert, J.. (2015). Diversity in extinction laws of Type Ia supernovae measured between 0.2 and 2 mu m. in Monthly Notices of the Royal Astronomical Society
Oxford Univ Press, Oxford., 453(3), 3300-3328.
https://doi.org/10.1093/mnras/stv1505

Amanullah R, Johansson J, Goobar A, Ferretti R, Papadogiannakis S, Petrushevska T, Brown PJ, Cao Y, Contreras C, Dahle H, Elias-Rosa N, Fynbo JPU, Gorosabel J, Guaita L, Hangard L, Howell DA, Hsiao EY, Kankare E, Kasliwal M, Leloudas G, Lundqvist P, Mattila S, Nugent P, Phillips MM, Sandberg A, Stanishev V, Sullivan M, Taddia F, Ostlin G, Asadi S, Herrero-Illana R, Jensen JJ, Karhunen K, Lazarević S, Varenius E, Santos P, Sridhar SS, Wallstrom SHJ, Wiegert J. Diversity in extinction laws of Type Ia supernovae measured between 0.2 and 2 mu m. in Monthly Notices of the Royal Astronomical Society. 2015;453(3):3300-3328.
doi:10.1093/mnras/stv1505 .

Amanullah, R., Johansson, J., Goobar, A., Ferretti, R., Papadogiannakis, S., Petrushevska, T., Brown, P. J., Cao, Y., Contreras, C., Dahle, H., Elias-Rosa, N., Fynbo, J. P. U., Gorosabel, J., Guaita, L., Hangard, L., Howell, D. A., Hsiao, E. Y., Kankare, E., Kasliwal, M., Leloudas, G., Lundqvist, P., Mattila, S., Nugent, P., Phillips, M. M., Sandberg, A., Stanishev, V., Sullivan, M., Taddia, F., Ostlin, G., Asadi, S., Herrero-Illana, R., Jensen, J. J., Karhunen, K., Lazarević, Slavica, Varenius, E., Santos, P., Sridhar, S. Seethapuram, Wallstrom, S. H. J., Wiegert, J., "Diversity in extinction laws of Type Ia supernovae measured between 0.2 and 2 mu m" in Monthly Notices of the Royal Astronomical Society, 453, no. 3 (2015):3300-3328,
https://doi.org/10.1093/mnras/stv1505 . .