TY  - JOUR
AU  - Tolić, Ljiljana
AU  - Grujić, Svetlana
AU  - Mojović, Miloš
AU  - Jovanović, Miloš
AU  - Lubec, Gert
AU  - Bacić, Goran
AU  - Laušević, Mila
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3367
AB  - A selective, sensitive and fast method for extraction, identification and quantification of multifunctional drug anisomycin in various tissues and serum, based on liquid chromatography-tandem mass spectrometry, was developed, optimized and validated. The method was validated according to the FDA guidelines and generally demonstrated good selectivity, accuracy, precision, and stability of the analyte. In the optimized method, high extraction efficiency was achieved for tested tissues (heart, brain, spleen, kidney, liver and femoral muscle) and serum. The obtained values of lower limits of quantification (LLOQ, 1.0-11.0 ng g(-1)) and limits of detection (0.3-3.3 ng g(-1)) indicated that the method was suitable for determination of trace levels of anisomycin in the complex matrices. The method was linear (R-2  gt = 0.990) in the tested concentration range (LLOQ-2500 ng g-1). The developed method was successfully applied in the first study on in vivo pharmacokinetics and distribution of anisomycin in tissues and serum of Wistar albino rats following subcutaneous injection (150 mg kg(-1)). The peak concentration in most tissues was achieved within 3 h after injection. The highest anisomycin concentration was found in the brain and the lowest concentration was found in the serum.
PB  - Royal Society of Chemistry
T2  - RSC Advances
T1  - Determination of anisomycin in tissues and serum by LC-MS/MS: application to pharmacokinetic and distribution studies in rats
EP  - 92489
IS  - 95
SP  - 92479
VL  - 6
DO  - 10.1039/c6ra16083b
ER  - 

@article{
author = "Tolić, Ljiljana and Grujić, Svetlana and Mojović, Miloš and Jovanović, Miloš and Lubec, Gert and Bacić, Goran and Laušević, Mila",
year = "2016",
abstract = "A selective, sensitive and fast method for extraction, identification and quantification of multifunctional drug anisomycin in various tissues and serum, based on liquid chromatography-tandem mass spectrometry, was developed, optimized and validated. The method was validated according to the FDA guidelines and generally demonstrated good selectivity, accuracy, precision, and stability of the analyte. In the optimized method, high extraction efficiency was achieved for tested tissues (heart, brain, spleen, kidney, liver and femoral muscle) and serum. The obtained values of lower limits of quantification (LLOQ, 1.0-11.0 ng g(-1)) and limits of detection (0.3-3.3 ng g(-1)) indicated that the method was suitable for determination of trace levels of anisomycin in the complex matrices. The method was linear (R-2  gt = 0.990) in the tested concentration range (LLOQ-2500 ng g-1). The developed method was successfully applied in the first study on in vivo pharmacokinetics and distribution of anisomycin in tissues and serum of Wistar albino rats following subcutaneous injection (150 mg kg(-1)). The peak concentration in most tissues was achieved within 3 h after injection. The highest anisomycin concentration was found in the brain and the lowest concentration was found in the serum.",
publisher = "Royal Society of Chemistry",
journal = "RSC Advances",
title = "Determination of anisomycin in tissues and serum by LC-MS/MS: application to pharmacokinetic and distribution studies in rats",
pages = "92489-92479",
number = "95",
volume = "6",
doi = "10.1039/c6ra16083b"
}

Tolić, L., Grujić, S., Mojović, M., Jovanović, M., Lubec, G., Bacić, G.,& Laušević, M.. (2016). Determination of anisomycin in tissues and serum by LC-MS/MS: application to pharmacokinetic and distribution studies in rats. in RSC Advances
Royal Society of Chemistry., 6(95), 92479-92489.
https://doi.org/10.1039/c6ra16083b

Tolić L, Grujić S, Mojović M, Jovanović M, Lubec G, Bacić G, Laušević M. Determination of anisomycin in tissues and serum by LC-MS/MS: application to pharmacokinetic and distribution studies in rats. in RSC Advances. 2016;6(95):92479-92489.
doi:10.1039/c6ra16083b .

Tolić, Ljiljana, Grujić, Svetlana, Mojović, Miloš, Jovanović, Miloš, Lubec, Gert, Bacić, Goran, Laušević, Mila, "Determination of anisomycin in tissues and serum by LC-MS/MS: application to pharmacokinetic and distribution studies in rats" in RSC Advances, 6, no. 95 (2016):92479-92489,
https://doi.org/10.1039/c6ra16083b . .

TY  - JOUR
AU  - Prokić, Bogomir Bolka
AU  - Bačić, G.
AU  - Prokić, B.
AU  - Kalijadis, Ana
AU  - Todorović, Vera
AU  - Puškaš, Nela
AU  - Vidojević, D.
AU  - Laušević, Mila
AU  - Laušević, Z.
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5692
AB  - In modern medicine implants are very important and so is their design and choice of materials. Almost equally important is the choice of imaging technique used to in vivo monitor their fate and biocompatibility. The aim of this study was to evaluate the ability of magnetic resonance imaging (MRI) in monitoring the biocompatibility of two newly designed carbon fibers. We have analyzed the interaction of surface functionalized carbon fibers (basic and acidic) with muscle and subcutaneous tissues of rabbits. MRI techniques showed to be useful in longitudinal monitoring of the surrounding tissues, assessment of biocompatibility of new implants, and in the distinction of in vivo surgical edema from inflammation. Histopathology confirmed MRI results, thus showing that MRI has a great potential for in vivo studies of such materials.
AB  - U modernoj medicine implanti su veoma važni, kao i njihovo dizajniranje i izbor materijala. Isto tako je značajan i izbor tehnike kojom se prati in vivo njihova sudbina i biokompatibilnost. Cilj ove studije je bila procena mogućnosti praćenja biokompatibilnosti dva novo dizajnirana karbonska vlakna pomoću magnetic resonance imaging (MRI). Analizirali smo interakciju karbonskih vlakana sa funkcionalizovanom površinom (kiselinom i bazom) sa mišićnim i potkožnim tkivom kunića. MRI tehnika se pokazala uspešnom u toku praćenja reakcije okolnog tkiva, i procene biokompatibilnosti nove vrste implanata, jer je uspela da in vivo odvoji hirurški edem od inflamacije. Patohistološka analiza je potvrdila rezultate MRI pokazujući time da MRI ima veliki potencijal za in vivo studije takvih materijala.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - In vivo MRI biocompatibility evaluation of functionalized carbon fibers in reaction with soft tissues
T1  - In vivo MRI procena biokompatibilnosti funkcionalizovanih karbonskih vlakana u reakciji sa mekim tkivom
EP  - 696
IS  - 5-6
SP  - 683
VL  - 62
DO  - 10.2298/AVB1206683P
ER  - 

@article{
author = "Prokić, Bogomir Bolka and Bačić, G. and Prokić, B. and Kalijadis, Ana and Todorović, Vera and Puškaš, Nela and Vidojević, D. and Laušević, Mila and Laušević, Z.",
year = "2012",
abstract = "In modern medicine implants are very important and so is their design and choice of materials. Almost equally important is the choice of imaging technique used to in vivo monitor their fate and biocompatibility. The aim of this study was to evaluate the ability of magnetic resonance imaging (MRI) in monitoring the biocompatibility of two newly designed carbon fibers. We have analyzed the interaction of surface functionalized carbon fibers (basic and acidic) with muscle and subcutaneous tissues of rabbits. MRI techniques showed to be useful in longitudinal monitoring of the surrounding tissues, assessment of biocompatibility of new implants, and in the distinction of in vivo surgical edema from inflammation. Histopathology confirmed MRI results, thus showing that MRI has a great potential for in vivo studies of such materials., U modernoj medicine implanti su veoma važni, kao i njihovo dizajniranje i izbor materijala. Isto tako je značajan i izbor tehnike kojom se prati in vivo njihova sudbina i biokompatibilnost. Cilj ove studije je bila procena mogućnosti praćenja biokompatibilnosti dva novo dizajnirana karbonska vlakna pomoću magnetic resonance imaging (MRI). Analizirali smo interakciju karbonskih vlakana sa funkcionalizovanom površinom (kiselinom i bazom) sa mišićnim i potkožnim tkivom kunića. MRI tehnika se pokazala uspešnom u toku praćenja reakcije okolnog tkiva, i procene biokompatibilnosti nove vrste implanata, jer je uspela da in vivo odvoji hirurški edem od inflamacije. Patohistološka analiza je potvrdila rezultate MRI pokazujući time da MRI ima veliki potencijal za in vivo studije takvih materijala.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "In vivo MRI biocompatibility evaluation of functionalized carbon fibers in reaction with soft tissues, In vivo MRI procena biokompatibilnosti funkcionalizovanih karbonskih vlakana u reakciji sa mekim tkivom",
pages = "696-683",
number = "5-6",
volume = "62",
doi = "10.2298/AVB1206683P"
}

Prokić, B. B., Bačić, G., Prokić, B., Kalijadis, A., Todorović, V., Puškaš, N., Vidojević, D., Laušević, M.,& Laušević, Z.. (2012). In vivo MRI biocompatibility evaluation of functionalized carbon fibers in reaction with soft tissues. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(5-6), 683-696.
https://doi.org/10.2298/AVB1206683P

Prokić BB, Bačić G, Prokić B, Kalijadis A, Todorović V, Puškaš N, Vidojević D, Laušević M, Laušević Z. In vivo MRI biocompatibility evaluation of functionalized carbon fibers in reaction with soft tissues. in Acta Veterinaria-Beograd. 2012;62(5-6):683-696.
doi:10.2298/AVB1206683P .

Prokić, Bogomir Bolka, Bačić, G., Prokić, B., Kalijadis, Ana, Todorović, Vera, Puškaš, Nela, Vidojević, D., Laušević, Mila, Laušević, Z., "In vivo MRI biocompatibility evaluation of functionalized carbon fibers in reaction with soft tissues" in Acta Veterinaria-Beograd, 62, no. 5-6 (2012):683-696,
https://doi.org/10.2298/AVB1206683P . .