Diversity of the human gastrointestinal tract microbiota revisited
Апстракт
Since the early days of microbiology, more than a century ago, representatives of over 400 different microbial species have been isolated and fully characterized from human gastrointestinal samples. However, during the past decade molecular ecological studies based on ribosomal RNA (rRNA) sequences have revealed that cultivation has been able only to access a small fraction of the microbial diversity within the gastrointestinal tract. The increasing number of deposited rRNA sequences calls for the setting up a curated database that allows handling of the excessive degree of redundancy that threatens the usability of public databases. The integration of data from cultivation-based studies and molecular inventories of small subunit (SSU) rRNA diversity, presented here for the first time, provides a systematic framework of the microbial diversity in the human gastrointestinal tract of more than 1000 different species-level phylogenetic types (phylotypes). Such knowledge is essential for t...he design of high-throughput approaches such as phylogenetic DNA microarrays for the comprehensive analysis of gastrointestinal tract microbiota at multiple levels of taxonomic resolution. Development of such approaches is likely to be pivotal to generating novel insights in microbiota functionality in health and disease.
Извор:
Environmental Microbiology, 2007, 9, 9, 2125-2136Издавач:
- Wiley, Hoboken
DOI: 10.1111/j.1462-2920.2007.01369.x
ISSN: 1462-2912
PubMed: 17686012
WoS: 000248598400001
Scopus: 2-s2.0-34547683600
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Rajilić-Stojanović, Mirjana AU - Smidt, Hauke AU - de Vos, Willem M. PY - 2007 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1163 AB - Since the early days of microbiology, more than a century ago, representatives of over 400 different microbial species have been isolated and fully characterized from human gastrointestinal samples. However, during the past decade molecular ecological studies based on ribosomal RNA (rRNA) sequences have revealed that cultivation has been able only to access a small fraction of the microbial diversity within the gastrointestinal tract. The increasing number of deposited rRNA sequences calls for the setting up a curated database that allows handling of the excessive degree of redundancy that threatens the usability of public databases. The integration of data from cultivation-based studies and molecular inventories of small subunit (SSU) rRNA diversity, presented here for the first time, provides a systematic framework of the microbial diversity in the human gastrointestinal tract of more than 1000 different species-level phylogenetic types (phylotypes). Such knowledge is essential for the design of high-throughput approaches such as phylogenetic DNA microarrays for the comprehensive analysis of gastrointestinal tract microbiota at multiple levels of taxonomic resolution. Development of such approaches is likely to be pivotal to generating novel insights in microbiota functionality in health and disease. PB - Wiley, Hoboken T2 - Environmental Microbiology T1 - Diversity of the human gastrointestinal tract microbiota revisited EP - 2136 IS - 9 SP - 2125 VL - 9 DO - 10.1111/j.1462-2920.2007.01369.x ER -
@article{ author = "Rajilić-Stojanović, Mirjana and Smidt, Hauke and de Vos, Willem M.", year = "2007", abstract = "Since the early days of microbiology, more than a century ago, representatives of over 400 different microbial species have been isolated and fully characterized from human gastrointestinal samples. However, during the past decade molecular ecological studies based on ribosomal RNA (rRNA) sequences have revealed that cultivation has been able only to access a small fraction of the microbial diversity within the gastrointestinal tract. The increasing number of deposited rRNA sequences calls for the setting up a curated database that allows handling of the excessive degree of redundancy that threatens the usability of public databases. The integration of data from cultivation-based studies and molecular inventories of small subunit (SSU) rRNA diversity, presented here for the first time, provides a systematic framework of the microbial diversity in the human gastrointestinal tract of more than 1000 different species-level phylogenetic types (phylotypes). Such knowledge is essential for the design of high-throughput approaches such as phylogenetic DNA microarrays for the comprehensive analysis of gastrointestinal tract microbiota at multiple levels of taxonomic resolution. Development of such approaches is likely to be pivotal to generating novel insights in microbiota functionality in health and disease.", publisher = "Wiley, Hoboken", journal = "Environmental Microbiology", title = "Diversity of the human gastrointestinal tract microbiota revisited", pages = "2136-2125", number = "9", volume = "9", doi = "10.1111/j.1462-2920.2007.01369.x" }
Rajilić-Stojanović, M., Smidt, H.,& de Vos, W. M.. (2007). Diversity of the human gastrointestinal tract microbiota revisited. in Environmental Microbiology Wiley, Hoboken., 9(9), 2125-2136. https://doi.org/10.1111/j.1462-2920.2007.01369.x
Rajilić-Stojanović M, Smidt H, de Vos WM. Diversity of the human gastrointestinal tract microbiota revisited. in Environmental Microbiology. 2007;9(9):2125-2136. doi:10.1111/j.1462-2920.2007.01369.x .
Rajilić-Stojanović, Mirjana, Smidt, Hauke, de Vos, Willem M., "Diversity of the human gastrointestinal tract microbiota revisited" in Environmental Microbiology, 9, no. 9 (2007):2125-2136, https://doi.org/10.1111/j.1462-2920.2007.01369.x . .