Effect of hydrogels based on 2-hydroxyethyl methacrilate to chemiluminescent activity of rat peritoneal macrophages

Abstract

The aim of this study was to examine the effect of hydrogels different chemical construction and synthesized under different conditions, to oxidative activity of rat peritoneal macrophages. The hydrogels are synthesized by radiation and chemical polymerization of 2-hydroxyethylmethacrylate (HEMA), copolymerization of HEMA with itaconic acid (IA), and copolymerization of HEMA, IA with poly(alkylene glycol) (met)acrylates-Bisomers. After incubation materials with macrophages, the effect of hydrogels was examine. All of tested materials reduct chemiluminescent activity of zimozan stimulated macrophages. Radiation polimerized PHEMA shows high inhibitory effect than chemical polimerized PHEMA. Hydrogels with more itaconic acid show high reducity effect, too. Different conditions of polimerization show differences in reducity effects to chemiluminescent activity of macrophages only in definite (order) reference (relation) between components of copolymers.

U radu je ispitivan efekat hidrogelova različitog hemijskog sastava i sintetisanih pod različitim uslovima na oksidativnu aktivnost peritonelanih makrofaga pacova. Hidrogelovi su dobijeni termički (hemijski) i radijaciono indukovanom polimerizacijom 2-hidroksietil metakrilata (HEMA) kopolimerizacijom HEMA i itakonske kiseline (IK), kao i kopolimerizacijom HEMA, IK i poli(alkilen glikol) (met)akrilata-Bisomeri (BIS). Efekat hidrogelova je ispitivan posle inkubacije materijala sa makrofagima dobijenim peritonealnom lavažom. Svi ispitivani materijali redukuju hemiluminescentnu aktivnost makrofaga stimulisanih zimozanom. Radijaciono umreženi PHEMA pokazuje veći inhibitorni efekat od hemijski umreženog. Hidrogelovi sa više itakonske kiseline pokazuju veći redukujući efekat. Različit način umrežavanja pokazuje razlike u redukujućim efektima na hemiluminescentnu aktivnost makrofaga samo pri određenim molarnim odnosima komponenti kopolimera.