Characterization of Intestinal Isolates of Lactic Acid Bacteria with Potential Probiotic Ability from Premature Born Nursling
Нема приказа
Аутори
Dimitrijević, SuzanaNedović, Viktor
Bogicević, Biljana
Trsić, Nada
Petrović, Tanja
Nikšić, Miomir
Bugarski, Branko
Lacroix, Christophe
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Five samples of feces from premature born nursling were taken from Institute of Neonatology (Belgrade). Three different media based on MRS was used for isolation of intestinal isolates of lactic acid bacteria. From 25 obtained cultures nine were selected for further investigation on potential probiotic ability and compared with Lactobacillus rhamnosus GG as reference strain. Preliminary identification has been performed according morphological traits (Gram staining, cells shape and colony characteristics on agar plate). Isolates suspected to lactobacilli and bifidobacteria were tested for selected physiological and biochemical characteristics. Tests for investigation of potential probiotic ability comprised antimicrobial activity against wide range Of pathogens, sensitivity to simulated gastrointestinal tract conditions, and antibiotic resistance, The morphological and physiological profiles showed that all strains are Lactobacillus rhamnosus. There were some differences in their sugar... fermentation pattern as well as in their anti I microbial activities. All strain grown well in simulated gastrointestinal condition but their sensitivity was greater on bile acids and pancreatin compared with low pH (1, 2 and 3) and pepsin. Most isolates showed good probiotic potential with similar properties with referent strain Lactobacillus rhamnosus GG determined in vitro.
Кључне речи:
probiotic / intestinal isolates / premature born nurslingИзвор:
Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20, 2008, 1, 343-349Издавач:
- 2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists
Институција/група
Tehnološko-metalurški fakultetTY - CONF AU - Dimitrijević, Suzana AU - Nedović, Viktor AU - Bogicević, Biljana AU - Trsić, Nada AU - Petrović, Tanja AU - Nikšić, Miomir AU - Bugarski, Branko AU - Lacroix, Christophe PY - 2008 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1213 AB - Five samples of feces from premature born nursling were taken from Institute of Neonatology (Belgrade). Three different media based on MRS was used for isolation of intestinal isolates of lactic acid bacteria. From 25 obtained cultures nine were selected for further investigation on potential probiotic ability and compared with Lactobacillus rhamnosus GG as reference strain. Preliminary identification has been performed according morphological traits (Gram staining, cells shape and colony characteristics on agar plate). Isolates suspected to lactobacilli and bifidobacteria were tested for selected physiological and biochemical characteristics. Tests for investigation of potential probiotic ability comprised antimicrobial activity against wide range Of pathogens, sensitivity to simulated gastrointestinal tract conditions, and antibiotic resistance, The morphological and physiological profiles showed that all strains are Lactobacillus rhamnosus. There were some differences in their sugar fermentation pattern as well as in their anti I microbial activities. All strain grown well in simulated gastrointestinal condition but their sensitivity was greater on bile acids and pancreatin compared with low pH (1, 2 and 3) and pepsin. Most isolates showed good probiotic potential with similar properties with referent strain Lactobacillus rhamnosus GG determined in vitro. PB - 2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists C3 - Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20 T1 - Characterization of Intestinal Isolates of Lactic Acid Bacteria with Potential Probiotic Ability from Premature Born Nursling EP - 349 SP - 343 VL - 1 UR - https://hdl.handle.net/21.15107/rcub_technorep_1213 ER -
@conference{ author = "Dimitrijević, Suzana and Nedović, Viktor and Bogicević, Biljana and Trsić, Nada and Petrović, Tanja and Nikšić, Miomir and Bugarski, Branko and Lacroix, Christophe", year = "2008", abstract = "Five samples of feces from premature born nursling were taken from Institute of Neonatology (Belgrade). Three different media based on MRS was used for isolation of intestinal isolates of lactic acid bacteria. From 25 obtained cultures nine were selected for further investigation on potential probiotic ability and compared with Lactobacillus rhamnosus GG as reference strain. Preliminary identification has been performed according morphological traits (Gram staining, cells shape and colony characteristics on agar plate). Isolates suspected to lactobacilli and bifidobacteria were tested for selected physiological and biochemical characteristics. Tests for investigation of potential probiotic ability comprised antimicrobial activity against wide range Of pathogens, sensitivity to simulated gastrointestinal tract conditions, and antibiotic resistance, The morphological and physiological profiles showed that all strains are Lactobacillus rhamnosus. There were some differences in their sugar fermentation pattern as well as in their anti I microbial activities. All strain grown well in simulated gastrointestinal condition but their sensitivity was greater on bile acids and pancreatin compared with low pH (1, 2 and 3) and pepsin. Most isolates showed good probiotic potential with similar properties with referent strain Lactobacillus rhamnosus GG determined in vitro.", publisher = "2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists", journal = "Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20", title = "Characterization of Intestinal Isolates of Lactic Acid Bacteria with Potential Probiotic Ability from Premature Born Nursling", pages = "349-343", volume = "1", url = "https://hdl.handle.net/21.15107/rcub_technorep_1213" }
Dimitrijević, S., Nedović, V., Bogicević, B., Trsić, N., Petrović, T., Nikšić, M., Bugarski, B.,& Lacroix, C.. (2008). Characterization of Intestinal Isolates of Lactic Acid Bacteria with Potential Probiotic Ability from Premature Born Nursling. in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20 2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists., 1, 343-349. https://hdl.handle.net/21.15107/rcub_technorep_1213
Dimitrijević S, Nedović V, Bogicević B, Trsić N, Petrović T, Nikšić M, Bugarski B, Lacroix C. Characterization of Intestinal Isolates of Lactic Acid Bacteria with Potential Probiotic Ability from Premature Born Nursling. in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20. 2008;1:343-349. https://hdl.handle.net/21.15107/rcub_technorep_1213 .
Dimitrijević, Suzana, Nedović, Viktor, Bogicević, Biljana, Trsić, Nada, Petrović, Tanja, Nikšić, Miomir, Bugarski, Branko, Lacroix, Christophe, "Characterization of Intestinal Isolates of Lactic Acid Bacteria with Potential Probiotic Ability from Premature Born Nursling" in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20, 1 (2008):343-349, https://hdl.handle.net/21.15107/rcub_technorep_1213 .